ViewGene

Locus Name:
GeneID:
Location:

GeneID: 2574

TrivialName: PA14_46060

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_46060

PAO1 Ortholog Locus: PA1422

Sequence Length: 894

Protein Length: 297

Start: 4096789

Stop: 4095896

Strand: -

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAG04811.1|AE004571_10 (AE004571) GbuR [Pseudomonas aeruginosa PAO1] Identities = 296/297 (99%), Positives = 296/297 (99%)

Sequence:

GTGTCCAACGCCTTGCCCGACCTGCGCCTGTTGCGCATCTTCGCCGCGGTGGTGCGCCACCAGGGCTTCGCC
GCCGCCCAGCAGGAGTTGAACCTGTCGACCTCGGCGATCAGCACCTACATGAGCCAACTGGAGAACCAGCTC
GGCATCGTGCTCTGCCACCGTGGCCGCGGCGGCTTCAGCCTGACCAGCAAGGGCGAGCTGTTCCACCAGGAG
ACCCTGCGCATCCTCGGCGAACTGGAAGGCTTCGGACGCTACGCGCTGGCGTTGAAGGGCGAGCTGCGCGGC
ACCCTGAACCTCGGTGTGCTCGATTCCACGGTGAGCGACCCGGCGCTGCCGCTGGCCGAGGTGATCGGCGCC
TTCAGCCGCGAACACCCGGCGGTGCACCTGCACCTGCTGGTGCAGAGCCCCTACGAACTGCAACTGAGCGTG
CTGGAGAACCGCCAGGACCTGGCCATCGGCGCCTTTTCCAGTCGCATGAACGGACTGGTCTACCAGCCGCTG
TACCGCGAACAGCACTGGCTGTACTGCAGCGACCGGCATCCGCTGTTCGACGGGCGGAGGATTCCCGCCGAC
CTGATCACCCAGCAACGCATGGTCGGCCGCGGCTACTGGAGCCAGGCCGAGCTGGCCCGGCACGGCTTCAAG
CACAGCGCGGGGACGGTGGAGAGCATGGAGGCGCAACTGATCCTGATCCTGTCCGGCGGCTACATCGGCTAC
CTGCCGGAGCACTACGCCCACCCGTGGGTCGAGCAGGGCCGTCTGCGCGCCCTGCTGCCGGCGACCTTCGGC
TACCAGGCGCCATTCTCCCTGATCCTCCGCCGCGGCCGCTCGCGCGAGCCGCTGATCCAGGCCTTCCGCGAC
CAGTTGCGGACGCAGATGAGCCTGGCCTGA

Translation:

MSNALPDLRLLRIFAAVVRHQGFAAAQQELNLSTSAISTYMSQLENQLGIVLCHRGRGGFSLTSKGELFHQE
TLRILGELEGFGRYALALKGELRGTLNLGVLDSTVSDPALPLAEVIGAFSREHPAVHLHLLVQSPYELQLSV
LENRQDLAIGAFSSRMNGLVYQPLYREQHWLYCSDRHPLFDGRRIPADLITQQRMVGRGYWSQAELARHGFK
HSAGTVESMEAQLILILSGGYIGYLPEHYAHPWVEQGRLRALLPATFGYQAPFSLILRRGRSREPLIQAFRD
QLRTQMSLA*

AnnotationID:2582 GeneID:2574

AnnotatorUID: diggins

Modification Date/Time: 2005-02-24 07:07:14

Gene Name: gbuR

Confidence Code: 1

GeneProduct: transcriptional regulator

Cell Localization: ()

Synonyms:

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (25) Transcriptional regulators

Alternate Gene Product Name:

Functional Category Confidence Code: 5

COGs: COG0583,pfam03466,pfam00126

Secondary Functional Category(ies):

EC Number:

Status: ACTIVE

Pathway:

Homology:

>gb|AAN70096.1|AE016649_4 (AE016791) transcriptional regulator, LysR family [Pseudomonas
           putida KT2440]
          Length = 297

 Score =  489 bits (1260), Expect = e-138
 Identities = 240/296 (81%), Positives = 266/296 (89%)

Structural Features:

COG0583, LysR, Transcriptional regulator [Transcription]
CD-Length = 297 residues,  98.3% aligned
Score =  124 bits (311), Expect = 2e-29
Identities = 91/296 (30%), Positives = 123/296 (41%), Gaps = 10/296 (3%)

pfam03466, LysR_substrate, LysR substrate binding domain. 
CD-Length = 209 residues, 100.0% aligned
Score =  107 bits (269), Expect = 1e-24
Identities = 60/212 (28%), Positives = 87/212 (41%), Gaps = 10/212 (4%)

pfam00126, HTH_1, Bacterial regulatory helix-turn-helix protein, lysR family.
CD-Length = 60 residues, 100.0% aligned
Score = 60.5 bits (147), Expect = 2e-10
Identities = 26/60 (43%), Positives = 32/60 (53%)

Genomic Context:

Comment:

ReferenceID:14629

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:22296

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:37422

Author/Investigator(s):

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: Misc (text/plain)

Data: 2574_cdd.html

URL:

ReferenceID:37996

Author/Investigator(s): Nakada Y, Itoh Y.

Title: Characterization and regulation of the gbuA gene, encoding guanidinobutyrase in the arginine dehydrogenase pathway of Pseudomonas aeruginosa PAO1.

PubMed: 12029055

MedLine:

Source:

J Bacteriol. 2002 Jun;184(12):3377-84.

Reference Type: Journal

Data:

URL:	`http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12029055`

Homologs By Global Alignment

Gene ID:2574

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

4275 PA1422_tr translation of PA1422 297 99.66 99.66 0 1517.0

125676 gb|AAG04811.1|AE004571_10 (AE004571) GbuR [Pseudomonas aeruginosa PAO1] 297 99.32 99.66 0 1514.0

125677 gb|AAN70096.1|AE016649_4 (AE016791) transcriptional regulator, LysR family [Pseudomonas putida KT2440] 297 80.47 89.56 0 1256.0

4276 PA1145_tr translation of PA1145 313 39.61 52.71 29 517.0

125678 gb|AAG04534.1|AE004544_6 (AE004544) probable transcriptional regulator [Pseudomonas aeruginosa PAO1] 313 39.61 52.71 29 517.0

GC ORFID: `45576`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA1422`	Glimmer Score:
Start: `857120`	Stop: `858013`
Length: `894`
Start Codon: `GTG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_PA1422 translation of PA1422`	Homolog Bit Score `588.0`
Other Homologs: `t_PA1145 (ORF: BLASTX 204.0), t_PA1422 (ORF: GLIMMER)`
GC ORF Sequence
GTGTCCAACGCCTTGCCCGACCTGCGCCTGTTGCGCATCTTCGCCGCGGTGGTGCGCCACCAGGGCTTCGCC GCCGCCCAGCAGGAGTTGAACCTGTCGACCTCGGCGATCAGCACCTACATGAGCCAACTGGAGAACCAGCTC GGCATCGTGCTCTGCCACCGTGGCCGCGGCGGCTTCAGCCTGACCAGCAAGGGCGAGCTGTTCCACCAGGAG ACCCTGCGCATCCTCGGCGAACTGGAAGGCTTCGGACGCTACGCGCTGGCGTTGAAGGGCGAGCTGCGCGGC ACCCTGAACCTCGGTGTGCTCGATTCCACGGTGAGCGACCCGGCGCTGCCGCTGGCCGAGGTGATCGGCGCC TTCAGCCGCGAACACCCGGCGGTGCACCTGCACCTGCTGGTGCAGAGCCCCTACGAACTGCAACTGAGCGTG CTGGAGAACCGCCAGGACCTGGCCATCGGCGCCTTTTCCAGTCGCATGAACGGACTGGTCTACCAGCCGCTG TACCGCGAACAGCACTGGCTGTACTGCAGCGACCGGCATCCGCTGTTCGACGGGCGGAGGATTCCCGCCGAC CTGATCACCCAGCAACGCATGGTCGGCCGCGGCTACTGGAGCCAGGCCGAGCTGGCCCGGCACGGCTTCAAG CACAGCGCGGGGACGGTGGAGAGCATGGAGGCGCAACTGATCCTGATCCTGTCCGGCGGCTACATCGGCTAC CTGCCGGAGCACTACGCCCACCCGTGGGTCGAGCAGGGCCGTCTGCGCGCCCTGCTGCCGGCGACCTTCGGC TACCAGGCGCCATTCTCCCTGATCCTCCGCCGCGGCCGCTCGCGCGAGCCGCTGATCCAGGCCTTCCGCGAC CAGTTGCGGACGCAGATGAGCCTGGCCTGA