ViewGene
Locus Name:
GeneID:
Location:

GeneID:2560
TrivialName: PA14_57260
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57260
PAO1 Ortholog Locus:PA4406
Sequence Length:912
Protein Length:303
Start: 5102745
Stop: 5101834
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07794.1|AE004856_5 (AE004856) UDP-3-O-acyl-N-acetylglucosamine deacetylase [Pseudomonas aeruginosa PAO1]
Identities = 302/303 (99%), Positives = 303/303 (100%)
Sequence:
ATGATCAAACAACGCACCTTGAAGAACATCATCCGGGCTACTGGCGTCGGTCTGCACTCGGGGGAAAAGGTT
TACCTGACCCTGAAACCGGCGCCGATGGACACCGGTATCGTGTTCTGCCGCACCGACCTGGATCCGGTAGTG
GAAATCCCGGCCCGGGCCGAGAACGTCGGCGAAACCACCATGTCGACCACCCTGGTCAAGGGTGACGTCAAA
GTGGATACGGTGGAGCACCTGCTCTCGGCCATGGCCGGCCTGGGTATCGACAACGCCTACGTCGAGCTGTCC
GCTTCGGAAGTGCCGATCATGGATGGCAGCGCTGGTCCGTTCGTATTCCTGATCCAGTCCGCCGGATTGCAA
GAGCAGGAAGCTGCCAAGAAGTTCATCCGCATCAAGCGCGAAGTCAGCGTGGAAGAGGGCGACAAGCGCGCC
GTCTTCGTTCCGTTCGACGGCTTCAAGGTCAGCTTCGAGATCGATTTCGATCATCCGGTCTTCCGTGGTCGC
ACCCAGCAGGCCTCGGTCGATTTCTCCAGTACTTCCTTCGTCAAGGAGGTCAGCCGCGCCCGTACCTTCGGG
TTCATGCGCGACATCGAGTACCTGCGTTCGCAGAACCTGGCACTCGGCGGTAGCGTGGAGAACGCGATCGTG
GTCGACGAGAACCGCGTGCTCAACGAAGACGGCCTGCGTTACGAGGACGAGTTCGTCAAGCACAAGATCCTG
GATGCCATCGGCGACCTGTATCTGCTCGGCAACAGTCTTATCGGCGAGTTCCGTGGCTTCAAGTCCGGCCAT
GCCCTGAACAACCAACTGCTGCGTACGTTGATCGCAGACAAGGATGCTTGGGAAGTGGTGACCTTCGAAGAC
GCGCGTACCGCGCCTATTTCCTATATGCGCCCGGCGGCGGCAGTGTAG
Translation:
MIKQRTLKNIIRATGVGLHSGEKVYLTLKPAPMDTGIVFCRTDLDPVVEIPARAENVGETTMSTTLVKGDVK
VDTVEHLLSAMAGLGIDNAYVELSASEVPIMDGSAGPFVFLIQSAGLQEQEAAKKFIRIKREVSVEEGDKRA
VFVPFDGFKVSFEIDFDHPVFRGRTQQASVDFSSTSFVKEVSRARTFGFMRDIEYLRSQNLALGGSVENAIV
VDENRVLNEDGLRYEDEFVKHKILDAIGDLYLLGNSLIGEFRGFKSGHALNNQLLRTLIADKDAWEVVTFED
ARTAPISYMRPAAAV*
AnnotationID:2568GeneID:2560
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 14:02:13
Gene Name:lpxC
Confidence Code:1
GeneProduct:UDP-3-O-acyl-N-acetylglucosamine deacetylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0774,pfam03331
Secondary Functional Category(ies):
EC Number:3.5.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66966.1|AE016325_2 (AE016779) UDP-3-0-acyl N-acetylglucosamine deacetylase
           [Pseudomonas putida KT2440]
          Length = 303

 Score =  541 bits (1394), Expect = e-153
 Identities = 277/303 (91%), Positives = 286/303 (94%)


>gb|AAO57851.1| (AE016871) UDP-3-0-acyl N-acetylglucosamine deacetylase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 303

 Score =  536 bits (1382), Expect = e-152
 Identities = 273/303 (90%), Positives = 285/303 (94%)








 
Structural Features:
COG0774, LpxC, UDP-3-O-acyl-N-acetylglucosamine deacetylase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 300 residues, 100.0% aligned
Score =  458 bits (1180), Expect = 7e-130
Identities = 193/302 (63%), Positives = 242/302 (80%), Gaps = 3/302 (0%)

pfam03331, LpxC, UDP-3-O-acyl N-acetylglycosamine deacetylase. 
CD-Length = 277 residues, 100.0% aligned
Score =  418 bits (1076), Expect = 1e-117
Identities = 166/278 (59%), Positives = 208/278 (74%), Gaps = 1/278 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5123




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22268




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:28237




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2560_cdd.html


    		




 
URL:

















    ReferenceID:32787




  
Author/Investigator(s):
        Kline T, Andersen NH, Harwood EA, Bowman J, Malanda A, Endsley S, Erwin AL, Doyle M, Fong S, Harris AL, Mendelsohn B, Mdluli K, Raetz CR, Stover CK, Witte PR, Yabannavar A, Zhu S.





  
Title:
        Potent, novel in vitro inhibitors of the Pseudomonas aeruginosa deacetylase LpxC.




  
PubMed:
        12086497


MedLine:
        




    
Source:
J Med Chem. 2002 Jul 4;45(14):3112-29.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12086497

















    ReferenceID:32788




  
Author/Investigator(s):
        Azzolina BA, Yuan X, Anderson MS, El-Sherbeini M.





  
Title:
        The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: cloning, production, and purification of active enzymes.




  
PubMed:
        11281713


MedLine:
        




    
Source:
Protein Expr Purif. 2001 Apr;21(3):393-400.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11281713

















    ReferenceID:32789




  
Author/Investigator(s):
        Hyland SA, Eveland SS, Anderson MS.





  
Title:
        Cloning, expression, and purification of UDP-3-O-acyl-GlcNAc deacetylase from Pseudomonas aeruginosa: a metalloamidase of the lipid A biosynthesis pathway.




  
PubMed:
        9068651


MedLine:
        




    
Source:
J Bacteriol. 1997 Mar;179(6):2029-37.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9068651

















    ReferenceID:32790




  
Author/Investigator(s):
        Pirrung MC, Tumey LN, McClerren AL, Raetz CR.





  
Title:
        High-throughput catch-and-release synthesis of oxazoline hydroxamates. Structure-activity relationships in novel inhibitors of Escherichia coli LpxC: in vitro enzyme inhibition and antibacterial properties.




  
PubMed:
        12568618


MedLine:
        




    
Source:
J Am Chem Soc. 2003 Feb 12;125(6):1575-86.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12568618









           
Homologs By Global Alignment 

           

       



     
Gene ID:2560
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4258
                           PA4406_tr
                           translation of PA4406
                           303
                           99.66
                           99.99
                           0
                           1521.0
     

     
     
125355
                           gb|AAG07794.1|AE004856_5
                           (AE004856) UDP-3-O-acyl-N-acetylglucosamine deacetylase [Pseudomonas aeruginosa PAO1]
                           303
                           99.66
                           99.99
                           0
                           1521.0
     

     
     
125356
                           gb|AAA95994.2|
                           (U19797) UDP-3-0-acyl N-acetylglucosamine deacetylase [Pseudomonas aeruginosa]
                           303
                           99.66
                           99.99
                           0
                           1521.0
     

     
     
125357
                           gb|AAC44974.1|
                           (U67855) UDP-3-O-acyl-GlcNAc deacetylase [Pseudomonas aeruginosa]
                           303
                           99.66
                           99.99
                           0
                           1521.0
     

     
     
125358
                           gb|AAN66966.1|AE016325_2
                           (AE016779) UDP-3-0-acyl N-acetylglucosamine deacetylase [Pseudomonas putida KT2440]
                           303
                           91.41
                           94.38
                           0
                           1394.0
     

     






      

          
GC ORFID: 45562How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4406Glimmer Score: 

          
Start: 4366421Stop: 4365510

          
Length: 912


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4406 translation of PA4406Homolog Bit Score 590.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1134 (ORF: BLASTX 541.0), t_PA4406 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCAAACAACGCACCTTGAAGAACATCATCCGGGCTACTGGCGTCGGTCTGCACTCGGGGGAAAAGGTT 
TACCTGACCCTGAAACCGGCGCCGATGGACACCGGTATCGTGTTCTGCCGCACCGACCTGGATCCGGTAGTG 
GAAATCCCGGCCCGGGCCGAGAACGTCGGCGAAACCACCATGTCGACCACCCTGGTCAAGGGTGACGTCAAA 
GTGGATACGGTGGAGCACCTGCTCTCGGCCATGGCCGGCCTGGGTATCGACAACGCCTACGTCGAGCTGTCC 
GCTTCGGAAGTGCCGATCATGGATGGCAGCGCTGGTCCGTTCGTATTCCTGATCCAGTCCGCCGGATTGCAA 
GAGCAGGAAGCTGCCAAGAAGTTCATCCGCATCAAGCGCGAAGTCAGCGTGGAAGAGGGCGACAAGCGCGCC 
GTCTTCGTTCCGTTCGACGGCTTCAAGGTCAGCTTCGAGATCGATTTCGATCATCCGGTCTTCCGTGGTCGC 
ACCCAGCAGGCCTCGGTCGATTTCTCCAGTACTTCCTTCGTCAAGGAGGTCAGCCGCGCCCGTACCTTCGGG 
TTCATGCGCGACATCGAGTACCTGCGTTCGCAGAACCTGGCACTCGGCGGTAGCGTGGAGAACGCGATCGTG 
GTCGACGAGAACCGCGTGCTCAACGAAGACGGCCTGCGTTACGAGGACGAGTTCGTCAAGCACAAGATCCTG 
GATGCCATCGGCGACCTGTATCTGCTCGGCAACAGTCTTATCGGCGAGTTCCGTGGCTTCAAGTCCGGCCAT 
GCCCTGAACAACCAACTGCTGCGTACGTTGATCGCAGACAAGGATGCTTGGGAAGTGGTGACCTTCGAAGAC 
GCGCGTACCGCGCCTATTTCCTATATGCGCCCGGCGGCGGCAGTGTAG