ViewGene
Locus Name:
GeneID:
Location:

GeneID:2559
TrivialName: PA14_56060
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56060
PAO1 Ortholog Locus:PA4314
Sequence Length:852
Protein Length:283
Start: 5006988
Stop: 5006137
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07702.1|AE004848_1 (AE004848) formyltetrahydrofolate deformylase [Pseudomonas aeruginosa PAO1]
Identities = 283/283 (100%), Positives = 283/283 (100%)
Sequence:
ATGCGCACTTTTCGGCTGGTGATCGCCTGTCCCGACGGGGTCGGGATCGTTGCCAAGGTGAGTAACTTCCTG
GCCACCTACAATGGCTGGATCACCGAAGCCAGCCATCATTCCGACAACGATAACGGCTGGTTCTTCATGCGC
CACGAGATCCGTGCCGACTCTCTGCCGTTCGACCTGGATGGCTTCCGCCAGGCTTTCGCCCCGATCGCCCGC
GAATTCTCCATGGAATGGCGGATCACCGATTCCGAGGTGAAGAAGCGCGTGGTGCTGATGGCCAGCAAGGAG
TCCCATTGCCTGGCCGATCTGCTGCACCGCTGGCACAGCGGCGAACTGGACTGCGAGATTCCCTGCGTGATC
GCCAACCACGACGACCTGCGCAGCATGGTCGAGTGGCACGGTATCCCGTATTTCCACGTTCCCGTCGATCCG
CAGGACAAGCAGCCGGCTTTCGATGAAGTTTCCCGGCTGATCGACGAACATGGCGCCGACTGCATCGTCCTG
GCTCGCTACATGCAGATCCTGCCGCCGGACCTGTGCCGCAAGTACGCCCACCAGGTGATCAACATCCACCAC
AGCTTCCTGCCGTCCTTCATCGGCGCCAAGCCGTATCACCAGGCGTCGAAGCGCGGGGTCAAGCTGATCGGC
GCGACCAGCCACTACGTGACCGAGGAACTGGATGCCGGTCCGATCATCGAGCAGGACGTGGTGCGGGTCACT
CACCGCGACAATGTCGAAGACATGGTGCGGCTGGGCAAGGACGTGGAAAAGCTGGTGCTCGCGCGCGGACTG
CGCTATCACCTGGAAGACCGCGTACTGGTACACGGCAACAAGACGGTGGTGTTCGACTGA
Translation:
MRTFRLVIACPDGVGIVAKVSNFLATYNGWITEASHHSDNDNGWFFMRHEIRADSLPFDLDGFRQAFAPIAR
EFSMEWRITDSEVKKRVVLMASKESHCLADLLHRWHSGELDCEIPCVIANHDDLRSMVEWHGIPYFHVPVDP
QDKQPAFDEVSRLIDEHGADCIVLARYMQILPPDLCRKYAHQVINIHHSFLPSFIGAKPYHQASKRGVKLIG
ATSHYVTEELDAGPIIEQDVVRVTHRDNVEDMVRLGKDVEKLVLARGLRYHLEDRVLVHGNKTVVFD*
AnnotationID:2567GeneID:2559
AnnotatorUID: diggins
Modification Date/Time: 2005-04-04 09:09:31
Gene Name:purU1
Confidence Code:2
GeneProduct:formyltetrahydrofolate deformylase
Cell Localization:()
Synonyms:purU
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0788,pfam00551
Secondary Functional Category(ies):
EC Number:3.5.1.10
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57765.1| (AE016871) formyltetrahydrofolate deformylase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 283

 Score =  508 bits (1308), Expect = e-143
 Identities = 240/283 (84%), Positives = 262/283 (92%)

>gb|AAN66990.1|AE016327_5 (AE016779) formyltetrahydrofolate deformylase [Pseudomonas putida
           KT2440]
          Length = 283

 Score =  505 bits (1300), Expect = e-142
 Identities = 237/283 (83%), Positives = 259/283 (91%)








 
Structural Features:
COG0788, PurU, Formyltetrahydrofolate hydrolase [Nucleotide transport and metabolism]
CD-Length = 287 residues,  98.6% aligned
Score =  448 bits (1155), Expect = 3e-127
Identities = 160/283 (56%), Positives = 204/283 (72%)

pfam00551, Formyl_trans_N, Formyl transferase. 
D-Length = 181 residues, 100.0% aligned
Score =  134 bits (338), Expect = 1e-32
Identities = 56/181 (30%), Positives = 79/181 (43%), Gaps = 3/181 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5121




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22266




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36677




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2559_cdd.html


    		




 
URL:

















    ReferenceID:36801




  
Author/Investigator(s):
        Nagy PL, McCorkle GM, Zalkin H.





  
Title:
        purU, a source of formate for purT-dependent phosphoribosyl-N-formylglycinamide synthesis.




  
PubMed:
        8226647


MedLine:
        




    
Source:
J Bacteriol. 1993 Nov;175(21):7066-73.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8226647









           
Homologs By Global Alignment 

           

       



     
Gene ID:2559
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4256
                           PA4314_tr
                           translation of PA4314
                           283
                           99.99
                           99.99
                           0
                           1520.0
     

     
     
125263
                           gb|AAG07702.1|AE004848_1
                           (AE004848) formyltetrahydrofolate deformylase [Pseudomonas aeruginosa PAO1]
                           283
                           99.99
                           99.99
                           0
                           1520.0
     

     
     
125264
                           gb|AAO57765.1|
                           (AE016871) formyltetrahydrofolate deformylase [Pseudomonas syringae pv. tomato str. DC3000]
                           283
                           84.80
                           92.57
                           0
                           1308.0
     

     
     
125265
                           gb|AAN66990.1|AE016327_5
                           (AE016779) formyltetrahydrofolate deformylase [Pseudomonas putida KT2440]
                           283
                           83.74
                           91.51
                           0
                           1300.0
     

     
     
125266
                           dbj|BAB97775.1|
                           (AP005275) Formyltetrahydrofolate hydrolase [Corynebacterium glutamicum ATCC 13032]
                           305
                           53.77
                           68.52
                           23
                           863.0
     

     






      

          
GC ORFID: 45561How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4314Glimmer Score: 

          
Start: 4270664Stop: 4269813

          
Length: 852


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4314 translation of PA4314Homolog Bit Score 590.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6186 (ORF: BLASTX 506.0), psyr_15may02_Scaffold1_revised_gene261 (ORF: BLASTX 260.0), t_PA5420 (ORF: BLASTX 243.0), t_PA4314 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCACTTTTCGGCTGGTGATCGCCTGTCCCGACGGGGTCGGGATCGTTGCCAAGGTGAGTAACTTCCTG 
GCCACCTACAATGGCTGGATCACCGAAGCCAGCCATCATTCCGACAACGATAACGGCTGGTTCTTCATGCGC 
CACGAGATCCGTGCCGACTCTCTGCCGTTCGACCTGGATGGCTTCCGCCAGGCTTTCGCCCCGATCGCCCGC 
GAATTCTCCATGGAATGGCGGATCACCGATTCCGAGGTGAAGAAGCGCGTGGTGCTGATGGCCAGCAAGGAG 
TCCCATTGCCTGGCCGATCTGCTGCACCGCTGGCACAGCGGCGAACTGGACTGCGAGATTCCCTGCGTGATC 
GCCAACCACGACGACCTGCGCAGCATGGTCGAGTGGCACGGTATCCCGTATTTCCACGTTCCCGTCGATCCG 
CAGGACAAGCAGCCGGCTTTCGATGAAGTTTCCCGGCTGATCGACGAACATGGCGCCGACTGCATCGTCCTG 
GCTCGCTACATGCAGATCCTGCCGCCGGACCTGTGCCGCAAGTACGCCCACCAGGTGATCAACATCCACCAC 
AGCTTCCTGCCGTCCTTCATCGGCGCCAAGCCGTATCACCAGGCGTCGAAGCGCGGGGTCAAGCTGATCGGC 
GCGACCAGCCACTACGTGACCGAGGAACTGGATGCCGGTCCGATCATCGAGCAGGACGTGGTGCGGGTCACT 
CACCGCGACAATGTCGAAGACATGGTGCGGCTGGGCAAGGACGTGGAAAAGCTGGTGCTCGCGCGCGGACTG 
CGCTATCACCTGGAAGACCGCGTACTGGTACACGGCAACAAGACGGTGGTGTTCGACTGA