ViewGene
Locus Name:
GeneID:
Location:

GeneID:2551
TrivialName: PA14_70730
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_70730
PAO1 Ortholog Locus:PA5358
Sequence Length:891
Protein Length:296
Start: 6298224
Stop: 6299114
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08743.1|AE004947_13 (AE004947) 4-hydroxybenzoate-octaprenyl transferase [Pseudomonas aeruginosa PAO1]
Identities = 293/296 (98%), Positives = 294/296 (99%)
Sequence:
ATGTTCGTCACCCTGATCAAGCCGCTGGCCCGCCTGCACCCCCGCGCCTGGGACTTCGTCCAACTGGTGCGG
CTGGACCGGCCGATCGGCATCTACCTGCTGCTCTGGCCGACCCTCTGGTCGCTGTGGATCGCCGCGGACGGC
GTGCCGGAGCTGAAGAACCTGCTGATCTTCGTCCTCgGCGTCATCCTGATGCGCGCCGCCGGCTGCGTGATC
AACGATTTCGCCGACCGCAATTTCGACGGCCACGTCGCACGCACCAAGGCGCGCCCGCTGGCCACCGGCAAG
ATCAGCGTGCGCGAGGCCTGGATCACCTTCGCCGTCCTGGTCGCGCTGAGCTTCGGCCTGGTCCTGCTGACC
AACGCAACGACCGTCTGGCTATCGTTCGGCGCGGTAGCCGTGGCCTCGCTCTACCCCTTCATGAAGCGCTAC
ACCTACTATCCGCAGGTGGTGCTGGGCGCCGCCTATTCCTGGGGCATCCTGATGGCCTTCACCGCCGAGCGC
GGTGAACTGCCGGCCAGCGCCTGGCTGCTGTTCCTGGCCAACGTGCTATGGACGGTCGCCTACGACTCCTAC
TACGCGATGACCGACCGCGAGGACGACCTGAAGATCGGCATCAAGTCCACCGCGATCCTCTTCGGCGATGCC
GACCGCCTGATCATCGGCAGCCTGCAGGGCCTGACCCTGCTCCTGCTGGCGCTGGCCGGCAGCCGCTTCGAA
CTGGGCCTGTACTTCTACCTCGGCCTGGCGGTGGCGGCGGCCTGCTTCGTCTGGGAAGCCTGGTCGACCCGC
GACCGCGACCCGCAGGCCTGCTTCCGCGCCTTCCTGCACAACCACTGGGCTGGCCTGGCGATCTTCCTCGGC
ACGGTCGCCGACTACGCGCTGCGTTGA
Translation:
MFVTLIKPLARLHPRAWDFVQLVRLDRPIGIYLLLWPTLWSLWIAADGVPELKNLLIFVLGVILMRAAGCVI
NDFADRNFDGHVARTKARPLATGKISVREAWITFAVLVALSFGLVLLTNATTVWLSFGAVAVASLYPFMKRY
TYYPQVVLGAAYSWGILMAFTAERGELPASAWLLFLANVLWTVAYDSYYAMTDREDDLKIGIKSTAILFGDA
DRLIIGSLQGLTLLLLALAGSRFELGLYFYLGLAVAAACFVWEAWSTRDRDPQACFRAFLHNHWAGLAIFLG
TVADYALR*
AnnotationID:2559GeneID:2551
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 19:07:07
Gene Name:ubiA
Confidence Code:2
GeneProduct:4-hydroxybenzoate polyprenyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0382,pfam01040,COG0109
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:2.5.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58895.1| (AE016875) 4-hydroxybenzoate polyprenyl transferase [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 296

 Score =  466 bits (1199), Expect = e-131
 Identities = 221/296 (74%), Positives = 255/296 (86%)

>gb|AAN70883.1|AE016732_8 (AE016794) 4-hydroxybenzoate polyprenyl transferase [Pseudomonas
           putida KT2440]
          Length = 296

 Score =  439 bits (1128), Expect = e-122
 Identities = 213/296 (71%), Positives = 246/296 (83%)

>gb|AAM84157.1|AE013659_1 (AE013659) 4-hydroxybenzoate-octaprenyltransferase [Yersinia pestis
           KIM]
          Length = 288

 Score =  319 bits (818), Expect = 1e-86
 Identities = 156/282 (55%), Positives = 200/282 (70%)






 
Structural Features:
COG0382, UbiA, 4-hydroxybenzoate polyprenyltransferase and related prenyltransferases 
[Coenzyme metabolism]
CD-Length = 289 residues,  99.7% aligned
Score =  202 bits (514), Expect = 6e-53
Identities = 125/288 (43%), Positives = 160/288 (55%), Gaps = 1/288 (0%)

pfam01040, UbiA, UbiA prenyltransferase family.
CD-Length = 269 residues, 100.0% aligned
Score =  136 bits (343), Expect = 4e-33
Identities = 97/269 (36%), Positives = 130/269 (48%), Gaps = 6/269 (2%)

COG0109, CyoE, Polyprenyltransferase (cytochrome oxidase assembly factor) 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 304 residues,  95.1% aligned
Score = 94.9 bits (236), Expect = 1e-20
Identities = 78/292 (26%), Positives = 118/292 (40%), Gaps = 8/292 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5105




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22250




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34755




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2551_cdd.html


    		




 
URL:

















    ReferenceID:35678




  
Author/Investigator(s):
        Wu G, Williams HD, Gibson F, Poole RK.





  
Title:
        Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase.




  
PubMed:
        8409922


MedLine:
        




    
Source:
J Gen Microbiol. 1993 Aug;139 ( Pt 8):1795-805.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8409922









           
Homologs By Global Alignment 

           

       



     
Gene ID:2551
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4245
                           PA5358_tr
                           translation of PA5358
                           296
                           98.98
                           99.32
                           0
                           1524.0
     

     
     
124985
                           gb|AAG08743.1|AE004947_13
                           (AE004947) 4-hydroxybenzoate-octaprenyl transferase [Pseudomonas aeruginosa PAO1]
                           296
                           98.98
                           99.32
                           0
                           1524.0
     

     
     
124986
                           gb|AAO58895.1|
                           (AE016875) 4-hydroxybenzoate polyprenyl transferase [Pseudomonas syringae pv. tomato str. DC3000]
                           296
                           74.66
                           86.14
                           0
                           1199.0
     

     
     
124987
                           gb|AAN70883.1|AE016732_8
                           (AE016794) 4-hydroxybenzoate polyprenyl transferase [Pseudomonas putida KT2440]
                           296
                           71.95
                           83.10
                           0
                           1128.0
     

     
     
124988
                           gb|AAM84157.1|AE013659_1
                           (AE013659) 4-hydroxybenzoate-octaprenyltransferase [Yersinia pestis KIM]
                           296
                           52.70
                           67.90
                           8
                           809.0
     

     






      

          
GC ORFID: 45553How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5358Glimmer Score: 

          
Start: 5561913Stop: 5562803

          
Length: 891


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5358 translation of PA5358Homolog Bit Score 591.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene2896 (ORF: BLASTX 458.0), t_PA5358 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTCGTCACCCTGATCAAGCCGCTGGCCCGCCTGCACCCCCGCGCCTGGGACTTCGTCCAACTGGTGCGG 
CTGGACCGGCCGATCGGCATCTACCTGCTGCTCTGGCCGACCCTCTGGTCGCTGTGGATCGCCGCGGACGGC 
GTGCCGGAGCTGAAGAACCTGCTGATCTTCGTCCTCgGCGTCATCCTGATGCGCGCCGCCGGCTGCGTGATC 
AACGATTTCGCCGACCGCAATTTCGACGGCCACGTCGCACGCACCAAGGCGCGCCCGCTGGCCACCGGCAAG 
ATCAGCGTGCGCGAGGCCTGGATCACCTTCGCCGTCCTGGTCGCGCTGAGCTTCGGCCTGGTCCTGCTGACC 
AACGCAACGACCGTCTGGCTATCGTTCGGCGCGGTAGCCGTGGCCTCGCTCTACCCCTTCATGAAGCGCTAC 
ACCTACTATCCGCAGGTGGTGCTGGGCGCCGCCTATTCCTGGGGCATCCTGATGGCCTTCACCGCCGAGCGC 
GGTGAACTGCCGGCCAGCGCCTGGCTGCTGTTCCTGGCCAACGTGCTATGGACGGTCGCCTACGACTCCTAC 
TACGCGATGACCGACCGCGAGGACGACCTGAAGATCGGCATCAAGTCCACCGCGATCCTCTTCGGCGATGCC 
GACCGCCTGATCATCGGCAGCCTGCAGGGCCTGACCCTGCTCCTGCTGGCGCTGGCCGGCAGCCGCTTCGAA 
CTGGGCCTGTACTTCTACCTCGGCCTGGCGGTGGCGGCGGCCTGCTTCGTCTGGGAAGCCTGGTCGACCCGC 
GACCGCGACCCGCAGGCCTGCTTCCGCGCCTTCCTGCACAACCACTGGGCTGGCCTGGCGATCTTCCTCGGC 
ACGGTCGCCGACTACGCGCTGCGTTGA