ViewGene
Locus Name:
GeneID:
Location:

GeneID:2544
TrivialName: PA14_38490
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_38490
PAO1 Ortholog Locus:PA2011
Sequence Length:903
Protein Length:300
Start: 3433535
Stop: 3434437
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05399.1|AE004627_7 (AE004627) hydroxymethylglutaryl-CoA lyase [Pseudomonas aeruginosa PAO1]
Identities = 300/300 (100%), Positives = 300/300 (100%)
Sequence:
ATGAACCTGCCGAAGAAGGTCCGCCTGGTGGAGGTCGGTCCGCGCGACGGACTGCAGAACGAGAAACAGCCG
ATCGAGGTGGCCGACAAGATCCGTCTGGTCGATGACCTGAGCGCCGCCGGCCTGGACTACATCGAAGTGGGC
AGCTTCGTCTCGCCCAAGTGGGTGCCGCAGATGGCCGGCTCGGCCGAGGTATTCGCCGGCATCCGCCAGCGT
CCCGGCGTGACCTACGCGGCGCTGGCGCCGAACCTGAAAGGCTTCGAGGCGGCGCTGGAGTCGGGAGTGAAG
GAAGTGGCGGTGTtcGCcgCCGCGTCGGAAGCCTTCTCCCAGCGCAACATCAACTGCTCGATCAAGGACAGC
CTGGAGCGCTTCGTACCGGTGCTGGAGGCGGCGCGCCAGCACCAGGTGCGGGTGCGCGGCTATATTTCCTGC
GTGCTCGGCTGTCCCTACGACGGCGATGTCGATCCGCGCCAGGTCGCCTGGGTCGCCCGCGAACTCCAGCAG
ATGGGCTGCTACGAAGTGTCCCTGGGCGACACCATCGGCGTCGGCACCGCCGGCGCCACCCGCCGGCTGATC
GAGGCGGTGGCCAGCGAGGTTCCCCGCGAGCGCCTCGCCGGGCACTTCCACGACACCTACGGGCAGGCCCTG
GCGAACATCTACGCCAGCCTGCTGGAAGGCATCGCGGTATTCGACAGCTCGGTGGCCGGCCTCGGCGGTTGT
CCCTACGCCAAGGGCGCCACCGGCAACGTCGCCAGCGAAGACGTCCTCTACCTGCTGAACGGTCTGGAGATC
CATACCGGGGTCGACATGCACGCCCTGGTCGACGCCGGCCAGCGCATCTGCGCGGTGCTCGGCAAGAGCAAC
GGATCGCGAGCGGCGAAGGCGTTGCTGGCCAAGGCCTGA
Translation:
MNLPKKVRLVEVGPRDGLQNEKQPIEVADKIRLVDDLSAAGLDYIEVGSFVSPKWVPQMAGSAEVFAGIRQR
PGVTYAALAPNLKGFEAALESGVKEVAVFAAASEAFSQRNINCSIKDSLERFVPVLEAARQHQVRVRGYISC
VLGCPYDGDVDPRQVAWVARELQQMGCYEVSLGDTIGVGTAGATRRLIEAVASEVPRERLAGHFHDTYGQAL
ANIYASLLEGIAVFDSSVAGLGGCPYAKGATGNVASEDVLYLLNGLEIHTGVDMHALVDAGQRICAVLGKSN
GSRAAKALLAKA*
AnnotationID:2552GeneID:2544
AnnotatorUID: danlee
Modification Date/Time: 2005-09-26 12:12:34
Gene Name:gnyL
Confidence Code:1
GeneProduct:3-hydroxy-gamma-carboxygeranoyl-CoA lyase, GnyL
Cell Localization:(1) Cytoplasmic
Synonyms:mvaB, hmgcL
Cell Localization Confidence Code:3
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00682
Secondary Functional Category(ies):
EC Number:4.3.1.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69141.1|AE016547_9 (AE016787) hydroxymethylglutaryl-CoA lyase [Pseudomonas putida
           KT2440]
          Length = 299

 Score =  474 bits (1220), Expect = e-133
 Identities = 235/299 (78%), Positives = 264/299 (88%)

>dbj|BAC62472.1| (AP005087) putative hydroxymethylglutaryl-CoA lyase [Vibrio
           parahaemolyticus]
          Length = 309

 Score =  362 bits (928), Expect = 2e-99
 Identities = 179/294 (60%), Positives = 223/294 (75%), Gaps = 1/294 (0%)

>gb|AAA25895.1| (M31807) HMG-CoA-lyase (mvaB) [Pseudomonas mevalonii]
          Length = 301

 Score =  361 bits (927), Expect = 3e-99
 Identities = 176/294 (59%), Positives = 222/294 (75%)








 
Structural Features:
pfam00682, HMGL-like, HMGL-like. 
CD-Length = 260 residues, 100.0% aligned
Score =  163 bits (413), Expect = 3e-41
Identities = 77/273 (28%), Positives = 120/273 (43%), Gaps = 14/273 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5091




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22236




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32719




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2544_cdd.html


    		




 
URL:

















    ReferenceID:34385




  
Author/Investigator(s):
        Anderson DH, Rodwell VW.





  
Title:
        Nucleotide sequence and expression in Escherichia coli of the 3-hydroxy-3-methylglutaryl coenzyme A lyase gene of Pseudomonas mevalonii.




  
PubMed:
        2687236


MedLine:
        




    
Source:
J Bacteriol. 1989 Dec;171(12):6468-72.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2687236









           
Homologs By Global Alignment 

           

       



     
Gene ID:2544
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4237
                           PA2011_tr
                           translation of PA2011
                           300
                           99.99
                           99.99
                           0
                           1523.0
     

     
     
124841
                           gb|AAG05399.1|AE004627_7
                           (AE004627) hydroxymethylglutaryl-CoA lyase [Pseudomonas aeruginosa PAO1]
                           300
                           99.99
                           99.99
                           0
                           1523.0
     

     
     
124842
                           gb|AAN69141.1|AE016547_9
                           (AE016787) hydroxymethylglutaryl-CoA lyase [Pseudomonas putida KT2440]
                           300
                           78.33
                           88.00
                           1
                           1220.0
     

     
     
124843
                           gb|AAO56243.1|
                           (AE016865) hydroxymethylglutaryl-CoA lyase [Pseudomonas syringae pv. tomato str. DC3000]
                           300
                           73.00
                           84.66
                           1
                           1137.0
     

     
     
124850
                           gb|AAM36713.1|
                           (AE011818) hydroxymethylglutaryl-CoA lyase [Xanthomonas axonopodis pv. citri str. 306]
                           300
                           58.66
                           72.33
                           2
                           900.0
     

     






      

          
GC ORFID: 45546How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2011Glimmer Score: 

          
Start: 1520376Stop: 1519474

          
Length: 903


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2011 translation of PA2011Homolog Bit Score 591.0

          
Other Homologs:  psyr_15may02_Scaffold9_revised_gene8067 (ORF: BLASTX 316.0), t_PA2011 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAACCTGCCGAAGAAGGTCCGCCTGGTGGAGGTCGGTCCGCGCGACGGACTGCAGAACGAGAAACAGCCG 
ATCGAGGTGGCCGACAAGATCCGTCTGGTCGATGACCTGAGCGCCGCCGGCCTGGACTACATCGAAGTGGGC 
AGCTTCGTCTCGCCCAAGTGGGTGCCGCAGATGGCCGGCTCGGCCGAGGTATTCGCCGGCATCCGCCAGCGT 
CCCGGCGTGACCTACGCGGCGCTGGCGCCGAACCTGAAAGGCTTCGAGGCGGCGCTGGAGTCGGGAGTGAAG 
GAAGTGGCGGTGTtcGCcgCCGCGTCGGAAGCCTTCTCCCAGCGCAACATCAACTGCTCGATCAAGGACAGC 
CTGGAGCGCTTCGTACCGGTGCTGGAGGCGGCGCGCCAGCACCAGGTGCGGGTGCGCGGCTATATTTCCTGC 
GTGCTCGGCTGTCCCTACGACGGCGATGTCGATCCGCGCCAGGTCGCCTGGGTCGCCCGCGAACTCCAGCAG 
ATGGGCTGCTACGAAGTGTCCCTGGGCGACACCATCGGCGTCGGCACCGCCGGCGCCACCCGCCGGCTGATC 
GAGGCGGTGGCCAGCGAGGTTCCCCGCGAGCGCCTCGCCGGGCACTTCCACGACACCTACGGGCAGGCCCTG 
GCGAACATCTACGCCAGCCTGCTGGAAGGCATCGCGGTATTCGACAGCTCGGTGGCCGGCCTCGGCGGTTGT 
CCCTACGCCAAGGGCGCCACCGGCAACGTCGCCAGCGAAGACGTCCTCTACCTGCTGAACGGTCTGGAGATC 
CATACCGGGGTCGACATGCACGCCCTGGTCGACGCCGGCCAGCGCATCTGCGCGGTGCTCGGCAAGAGCAAC 
GGATCGCGAGCGGCGAAGGCGTTGCTGGCCAAGGCCTGA