ViewGene
Locus Name:
GeneID:
Location:

GeneID:2543
TrivialName: PA14_05380
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05380
PAO1 Ortholog Locus:PA0412
Sequence Length:876
Protein Length:291
Start: 467977
Stop: 468852
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03801.1|AE004478_6 (AE004478) methyltransferase PilK [Pseudomonas aeruginosa PAO1]
Identities = 290/291 (99%), Positives = 290/291 (99%)
Sequence:
ATGCAGGCGAACGGCGTCTGGTCACTGCAGCCGCTGGCCGATATGTCGGCAGCGGAGTTCCGCGACTGGCAG
GTCCTGTTGGAGAACCGCACCGGCGTGGTGCTCAACGAGCAGCGCCGGACGTTCCTGCAGGCCAGCCTGACC
GCGCGCATGCGTGAACTGGGCATCGGCGACTACCACAGCTACTACCGCCAGGTGACCGACGGTCCGCGTGGC
GCAGTGGAGTGGGCGACCCTGCTGGACCGCCTGACCGTCCAGGAAACCCGTTTTTTCCGTCATCCCCCGTCT
TTCGAGCTGCTCGAGCGCTACCTGGGCGAGCGCCTGCGCCGCGAAGGCATGCCGCGGCCCTGGGCCCTGTGG
AGCGTCGGCTGCTCCAGCGGCGAGGAACCCTACTCGCTGGCGATGTGCGCCGCGCAGGCGTTGCGCGGGCAG
GAACGGGAAGATTTTTTCGGCGTCACCGGGACGGATATCAGCCTTCACGCCCTGCAGCGGGCGCGGCAGGCG
AACTATCCGGCCCGCAAGCTGGAGCAACTGGAGGCCGGACTGGTCGAACGCTATTGCGAACGCCAGGCCGAC
GGCAGCTTCAGCGTGAAAACGATACTGACCGAGCGCGTCTGCTGCGCCCGGCTGAATGTGCTGGACCTGGCG
AAGGCGCCCTGGTCCGGCATGGACGTGATTTTTTGTCAGAACCTGCTGATCTACTTCCGTCGCTGGCGACGG
CGCGAGATCCTCAACCGGTTGGCCGAACGGCTGGCGCCGGGAGGCTTGCTGGTGATCGGGGTCGGCGAAGTG
GTCGACTGGAGCCATCCGGAGCTCGAGCCGGTCGCCGACGAGCGGGTCCTGGCCTTTACCCGTAAGGGGTAC
TCAGGCACATGA
Translation:
MQANGVWSLQPLADMSAAEFRDWQVLLENRTGVVLNEQRRTFLQASLTARMRELGIGDYHSYYRQVTDGPRG
AVEWATLLDRLTVQETRFFRHPPSFELLERYLGERLRREGMPRPWALWSVGCSSGEEPYSLAMCAAQALRGQ
EREDFFGVTGTDISLHALQRARQANYPARKLEQLEAGLVERYCERQADGSFSVKTILTERVCCARLNVLDLA
KAPWSGMDVIFCQNLLIYFRRWRRREILNRLAERLAPGGLLVIGVGEVVDWSHPELEPVADERVLAFTRKGY
SGT*
AnnotationID:2551GeneID:2543
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 08:08:31
Gene Name:pilK
Confidence Code:1
GeneProduct:methyltransferase PilK
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(10) Chemotaxis
Alternate Gene Product Name:methylase of chemotaxis methyl-accepting proteins
Functional Category Confidence Code:5
COGs:smart00138,COG1352,pfam01739
Secondary Functional Category(ies):motility and attachment
EC Number:2.1.1.80
Status:ACTIVE
Pathway:






 
Homology:






 
Structural Features:
smart00138, MeTrc, Methyltransferase, chemotaxis proteins
CD-Length = 264 residues, 100.0% aligned
Score =  290 bits (745), Expect = 8e-80
Identities = 113/268 (42%), Positives = 150/268 (55%), Gaps = 5/268 (1%)

COG1352, CheR, Methylase of chemotaxis methyl-accepting proteins 
[Cell motility and secretion / Signal transduction mechanisms]
CD-Length = 268 residues,  99.6% aligned
Score =  259 bits (664), Expect = 2e-70
Identities = 95/272 (34%), Positives = 135/272 (49%), Gaps = 6/272 (2%)

pfam01739, CheR, CheR methyltransferase, SAM binding domain. 
CD-Length = 195 residues, 100.0% aligned
Score =  213 bits (545), Expect = 1e-56
Identities = 79/198 (39%), Positives = 103/198 (52%), Gaps = 3/198 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:5089




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22234




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33739




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2543_cdd.html


    		




 
URL:

















    ReferenceID:35370




  
Author/Investigator(s):
        Darzins A.





  
Title:
        The Pseudomonas aeruginosa pilK gene encodes a chemotactic methyltransferase (CheR) homologue that is translationally regulated.




  
PubMed:
        7783642


MedLine:
        




    
Source:
Mol Microbiol. 1995 Feb;15(4):703-17.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7783642

















    ReferenceID:35371




  
Author/Investigator(s):
        Sakai D, Komano T.





  
Title:
        Genes required for plasmid R64 thin-pilus biogenesis: identification and localization of products of the pilK, pilM, pilO, pilP, pilR, and pilT genes.




  
PubMed:
        11751821


MedLine:
        




    
Source:
J Bacteriol. 2002 Jan;184(2):444-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=11751821









           
Homologs By Global Alignment 

           

       



     
Gene ID:2543
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4236
                           PA0412_tr
                           translation of PA0412
                           291
                           99.65
                           99.65
                           0
                           1525.0
     

     
     
124839
                           gb|AAG03801.1|AE004478_6
                           (AE004478) methyltransferase PilK [Pseudomonas aeruginosa PAO1]
                           291
                           99.65
                           99.65
                           0
                           1525.0
     

     
     
124840
                           gb|AAA85790.1|
                           (U11382) similar to product encoded by chemotactic methyltransferase gene: GenBank Accession Number X73681 [Pseudomonas aeruginosa]
                           291
                           99.65
                           99.65
                           0
                           1525.0
     

     






      

          
GC ORFID: 45545How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0412Glimmer Score: 

          
Start: 6269319Stop: 6270194

          
Length: 876


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0412 translation of PA0412Homolog Bit Score 592.0

          
Other Homologs:  t_PA0412 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAGGCGAACGGCGTCTGGTCACTGCAGCCGCTGGCCGATATGTCGGCAGCGGAGTTCCGCGACTGGCAG 
GTCCTGTTGGAGAACCGCACCGGCGTGGTGCTCAACGAGCAGCGCCGGACGTTCCTGCAGGCCAGCCTGACC 
GCGCGCATGCGTGAACTGGGCATCGGCGACTACCACAGCTACTACCGCCAGGTGACCGACGGTCCGCGTGGC 
GCAGTGGAGTGGGCGACCCTGCTGGACCGCCTGACCGTCCAGGAAACCCGTTTTTTCCGTCATCCCCCGTCT 
TTCGAGCTGCTCGAGCGCTACCTGGGCGAGCGCCTGCGCCGCGAAGGCATGCCGCGGCCCTGGGCCCTGTGG 
AGCGTCGGCTGCTCCAGCGGCGAGGAACCCTACTCGCTGGCGATGTGCGCCGCGCAGGCGTTGCGCGGGCAG 
GAACGGGAAGATTTTTTCGGCGTCACCGGGACGGATATCAGCCTTCACGCCCTGCAGCGGGCGCGGCAGGCG 
AACTATCCGGCCCGCAAGCTGGAGCAACTGGAGGCCGGACTGGTCGAACGCTATTGCGAACGCCAGGCCGAC 
GGCAGCTTCAGCGTGAAAACGATACTGACCGAGCGCGTCTGCTGCGCCCGGCTGAATGTGCTGGACCTGGCG 
AAGGCGCCCTGGTCCGGCATGGACGTGATTTTTTGTCAGAACCTGCTGATCTACTTCCGTCGCTGGCGACGG 
CGCGAGATCCTCAACCGGTTGGCCGAACGGCTGGCGCCGGGAGGCTTGCTGGTGATCGGGGTCGGCGAAGTG 
GTCGACTGGAGCCATCCGGAGCTCGAGCCGGTCGCCGACGAGCGGGTCCTGGCCTTTACCCGTAAGGGGTAC 
TCAGGCACATGA