ViewGene
Locus Name:
GeneID:
Location:

GeneID:2531
TrivialName: PA14_67130
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_67130
PAO1 Ortholog Locus:PA5082
Sequence Length:900
Protein Length:299
Start: 5993233
Stop: 5992334
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08467.1|AE004921_4 (AE004921) probable binding protein component of ABC transporter [Pseudomonas aeruginosa PAO1]
Identities = 299/299 (100%), Positives = 299/299 (100%)
Sequence:
ATGAAAACCCTTGTATCCAGCACTTTCGCACTCTCCCTGTCGCTCGCCATCGGCGCCGCGCAGGCCGAGGAA
CTCAGCGGCACCCTGAAGAAGGTTGCCGACAACGGCACGATAACCCTGGGTTATCGCGATGCCTCGGTGCCT
TTCTCCTACCTTGGCGATAACAGCGGCCAGCCCATGGGCTATTCGGTGGAACTGGCCGGCAAGGTGGTGGAG
CGGCTCAAAAGCACCCTCGGCAAGCCGGAACTCAAGGTGAAGTACAACCTGGTCACTTCGCAGACTCGCATC
CCGCTGGTGCAGAACGGCACCGTCGACCTGGAGTGCGGCTCCACCGGGGTCACCGCCGAGCGGCAGAAACAG
GTGGCGTTCTCCTACGGCTTCATCTACGTCAAGGGCCAGTTGCTGACCCGCAAGGACAGCGGCATCGCCCAC
TTCGACGACCTCAAGGGCAAGAACGTGGTGACCACCGCCGGTACCACCAACGAGCGCTACCTGAAGAACTAC
AACGCCGAACACAAGCTGGGGATGAACGTGGTCAGCGCCAAGGACCACGGCGAAGCCTTCCAGATGCTGCAA
TCGGGTCGCGCGGTGGCCTTCTACATGGACGATGCGCTGCTCTACGGCGAACGCGCCAAGGCGCGCCAGCCG
CACGACTGGGTGGTGGTCGGCGAGCCGCAATCGCGGGAGATCTACAGCTGCATGGTGCGCAAGGACGACCCG
CAGTTCCTTGCCCTGGTCAACGAGACCCTGGCCGGGCTGTATCGCTCGGGCGAGATACAGGGCATCTACCAG
CGCTGGTTCCAGCAGCCGATCCCGCCCAAGGGGTTGAACCTGGAGTTCCCCATGACCGCGGAGCTGAAGGCG
ATCCTCGAGAACCCCACCAGCGATCCGGTGCAGTGA
Translation:
MKTLVSSTFALSLSLAIGAAQAEELSGTLKKVADNGTITLGYRDASVPFSYLGDNSGQPMGYSVELAGKVVE
RLKSTLGKPELKVKYNLVTSQTRIPLVQNGTVDLECGSTGVTAERQKQVAFSYGFIYVKGQLLTRKDSGIAH
FDDLKGKNVVTTAGTTNERYLKNYNAEHKLGMNVVSAKDHGEAFQMLQSGRAVAFYMDDALLYGERAKARQP
HDWVVVGEPQSREIYSCMVRKDDPQFLALVNETLAGLYRSGEIQGIYQRWFQQPIPPKGLNLEFPMTAELKA
ILENPTSDPVQ*
AnnotationID:2539GeneID:2531
AnnotatorUID: diggins
Modification Date/Time: 2005-03-15 06:06:08
Gene Name:
Confidence Code:3
GeneProduct:putative binding protein component of ABC transporter
Cell Localization:()
Synonyms:ybeJ
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00497,smart00062,cd00134,COG0834
Secondary Functional Category(ies):membrane protein
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57627.1| (AE016871) amino acid ABC transporter, periplasmic amino
           acid-binding protein [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 308

 Score =  365 bits (937), Expect = e-100
 Identities = 179/285 (62%), Positives = 219/285 (76%)

>gb|AAN66696.1|AE016297_10 (AE016778) amino acid ABC transporter, periplasmic amino
           acid-binding protein [Pseudomonas putida KT2440]
          Length = 306

 Score =  333 bits (853), Expect = 1e-90
 Identities = 173/288 (60%), Positives = 215/288 (74%), Gaps = 5/288 (1%)

>emb|CAD14009.1| (AL646059) PROBABLE AMINO-ACID-BINDING PERIPLASMIC (PBP) ABC
           TRANSPORTER PROTEIN [Ralstonia solanacearum]
          Length = 313

 Score =  329 bits (844), Expect = 1e-89
 Identities = 163/289 (56%), Positives = 217/289 (75%), Gaps = 5/289 (1%)






 
Structural Features:
pfam00497, SBP_bac_3, Bacterial extracellular solute-binding proteins, family 3.
CD-Length = 224 residues, 100.0% aligned
Score =  177 bits (450), Expect = 2e-45
Identities = 74/234 (31%), Positives = 110/234 (47%), Gaps = 12/234 (5%)

smart00062, PBPb, Bacterial periplasmic substrate-binding protein
CD-Length = 222 residues, 100.0% aligned
Score =  167 bits (423), Expect = 2e-42
Identities = 78/235 (33%), Positives = 113/235 (48%), Gaps = 14/235 (5%)

COG0834, HisJ, ABC-type amino acid transport/signal transduction systems, 
periplasmic component/domain 
[Amino acid transport and metabolism / Signal transduction mechanisms]
CD-Length = 275 residues,  96.0% aligned
Score =  166 bits (420), Expect = 3e-42
Identities = 84/271 (30%), Positives = 127/271 (46%), Gaps = 12/271 (4%)

cd00134, PBPb, Bacterial periplasmic transport system
CD-Length = 218 residues, 100.0% aligned
Score =  150 bits (380), Expect = 2e-37
Identities = 78/232 (33%), Positives = 112/232 (48%), Gaps = 16/232 (6%)







   
Genomic Context:






   
Comment:


















    ReferenceID:5065




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22211




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34990




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2531_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2531
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4219
                           PA5082_tr
                           translation of PA5082
                           299
                           99.99
                           99.99
                           0
                           1532.0
     

     
     
124419
                           gb|AAG08467.1|AE004921_4
                           (AE004921) probable binding protein component of ABC transporter [Pseudomonas aeruginosa PAO1]
                           299
                           99.99
                           99.99
                           0
                           1532.0
     

     
     
4220
                           PA1342_tr
                           translation of PA1342
                           304
                           60.52
                           74.01
                           7
                           937.0
     

     
     
124420
                           gb|AAG04731.1|AE004563_8
                           (AE004563) probable binding protein component of ABC transporter [Pseudomonas aeruginosa PAO1]
                           304
                           60.52
                           74.01
                           7
                           937.0
     

     
     
124422
                           gb|AAQ57799.1|
                           (AE016910) probable amino acid ABC transporter, periplasmic amino acid-binding protein [Chromobacterium violaceum ATCC 12472]
                           303
                           59.73
                           72.27
                           5
                           922.0
     

     






      

          
GC ORFID: 45533How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5082Glimmer Score: 

          
Start: 5256918Stop: 5256019

          
Length: 900


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5082 translation of PA5082Homolog Bit Score 594.0

          
Other Homologs:  t_PA1342 (ORF: BLASTX 366.0), psyr_15may02_Scaffold1_revised_gene299 (ORF: BLASTX 356.0), t_PA5082 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAACCCTTGTATCCAGCACTTTCGCACTCTCCCTGTCGCTCGCCATCGGCGCCGCGCAGGCCGAGGAA 
CTCAGCGGCACCCTGAAGAAGGTTGCCGACAACGGCACGATAACCCTGGGTTATCGCGATGCCTCGGTGCCT 
TTCTCCTACCTTGGCGATAACAGCGGCCAGCCCATGGGCTATTCGGTGGAACTGGCCGGCAAGGTGGTGGAG 
CGGCTCAAAAGCACCCTCGGCAAGCCGGAACTCAAGGTGAAGTACAACCTGGTCACTTCGCAGACTCGCATC 
CCGCTGGTGCAGAACGGCACCGTCGACCTGGAGTGCGGCTCCACCGGGGTCACCGCCGAGCGGCAGAAACAG 
GTGGCGTTCTCCTACGGCTTCATCTACGTCAAGGGCCAGTTGCTGACCCGCAAGGACAGCGGCATCGCCCAC 
TTCGACGACCTCAAGGGCAAGAACGTGGTGACCACCGCCGGTACCACCAACGAGCGCTACCTGAAGAACTAC 
AACGCCGAACACAAGCTGGGGATGAACGTGGTCAGCGCCAAGGACCACGGCGAAGCCTTCCAGATGCTGCAA 
TCGGGTCGCGCGGTGGCCTTCTACATGGACGATGCGCTGCTCTACGGCGAACGCGCCAAGGCGCGCCAGCCG 
CACGACTGGGTGGTGGTCGGCGAGCCGCAATCGCGGGAGATCTACAGCTGCATGGTGCGCAAGGACGACCCG 
CAGTTCCTTGCCCTGGTCAACGAGACCCTGGCCGGGCTGTATCGCTCGGGCGAGATACAGGGCATCTACCAG 
CGCTGGTTCCAGCAGCCGATCCCGCCCAAGGGGTTGAACCTGGAGTTCCCCATGACCGCGGAGCTGAAGGCG 
ATCCTCGAGAACCCCACCAGCGATCCGGTGCAGTGA