ViewGene
Locus Name:
GeneID:
Location:

GeneID:252
TrivialName: PA14_30180
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_30180
PAO1 Ortholog Locus:PA2624
Sequence Length:2226
Protein Length:741
Start: 2614749
Stop: 2612524
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06012.1|AE004691_3 (AE004691) isocitrate dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 738/741 (99%), Positives = 741/741 (100%)
Sequence:
ATGTCCATCCGCTCGAAGATCACCTACACCTTCACCGACGAAGCACCCGCGCTCGCCACCTACTCGCTCCTC
CCCATCGTCAAGGCCTTTGCCGCTTCCGCCGGCATCGACGTCGAGACCAGCGACATCTCTCTTGCAGGACGC
ATCCTGGCCAACTTCGCCGACCGCCTCGACGCCGACCAGCGTATCGAAGACGACCTCGCCCGCCTGGCGGTC
CTGGCCACCTCGCCCGACGCCAATATCATCAAGCTGCCCAACATCAGCGCCTCGGTGCCCCAGCTGAAAGGC
GCAATCGCCGAACTGCAGGGCCTGGGCTACAAGGTTCCCGACTTCCCGGAAGACCCGCAGACCGACGAAGAG
AAAGAGGTTCGCGCACGCTACGCGAAGATCCTCGGCAGCGCCGTGAACCCGGTCCTGCGCGAAGGCAACTCC
GACCGTCGCGCGCCTGCCGCGGTGAAGGCCTACGCCCGCAAGCACCCGCACTCCATGGGCAAGTGGAGCATG
GCCTCCCGCTCCCACGCCGACTACATGCGCGGCGGCGACTTCTTCTCCAGCGAACAGTCCATCACCATGGCC
AAGGCCGGTGACGTGCGCATCGAGTTCGTCGGCAAGGACGGCAAGGTCGAGGTCAAGAAGCAACTGAGCCTG
CAGGAAGGCGAAGTGCTCGACAGCATGTTCATGAGCTGCGGCAAGCTGCGCGACTTCTTCGAGAAGACCCTG
CAGGACTGCAAGGAAACCGGCGTCATGTGGTCCCTGCACGTCAAGGCGACCATGATGAAGATTTCCCACCCG
ATCGTCTTCGGGCATGCGGTGAGCGTCTACTACAAGGACGTCTTCGACAAGTGGGGCCAACTCTTCGAAGAG
CTCGGCGTGAACCCGAACAACGGTATCTCCAGCGTCTACGACAAGATCAAGTCGCTGCCCGCCTCCCAGCAG
GAAGAGATCCTCCACGACATCCACGAGGTGTACAGCCACCGCCCGGAAATGGCGATGGTCGACTCCGTGAAA
GGCATCACCAACCTGCACATTCCGAGCGACGTCATCGTCGACGCCTCGATGCCGGCGATGATCCGCAACTCC
GGCCAGATGTGGGGCAAGGACGGCAAGCAGAAAGATACCAAGGCGGTAATGCCGGAAAGCACCTACGCCCGC
ATCTACCAGGAGATGATCAACTTCTGCAAGACCAACGGCGCCTTCGACCCGACCACCATGGGCAGCGTGCCG
AACGTCGGCCTGATGGCGCAGAAGGCCGAGGAATACGGCTCCCACGACAAGACCTTCGAAATGACCGCCGAC
GGCACCATGCGCGTGGTCCTGGCCGACGGCAGCGTGCTGATGCAGCACGAGGTGGAGACAGGCGACATCTGG
CGCGCCTGCCAGACCAAGGACGCGCCGATCCGCGACTGGGTCAAGCTGGCCGTCACCCGCGCGCGCCAGTCC
GACACCCCGGCGATCTTCTGGCTGGACCCGGAGCGCGCCCACGACCGCGAACTGCGCAAGAAGGTCGAGCTG
TACCTCAAGGACCACGACCTGACCGGCCTCGACATCAGCATCATGGGCTACAACGAGGCCATTCGCGTCAGC
ATGGAACGCCTGATCCGCGGCAAGGACACCATCTCGGTGACCGGCAACGTCCTGCGCGACTACCTGACCGAC
CTGTTCCCGATCATGGAACTGGGCACCTCGGCCAAGATGCTCTCCATCGTTCCGCTGATGGCGGGCGGCGGC
ATGTACGAAACCGGCGCCGGCGGCTCGGCACCCAAGCACGTCCAGCAACTGGTGGAAGAGAACTACCTGCGC
TGGGACTCCCTGGGCGAGTTCCTGGCCCTGGCCGTGTCCCTGGAGGAAACCGGGATCAAGACCGGCAACGCC
AAGGCCAAGCTGCTCGGCAAGGCGCTGGACGAAGCCACCGGCAAGCTGCTGGACAACAACAAGTCGCCGTCG
CGCAAGGTCGGCGACATCGACAACCGCGGCAGCCACTTCTACCTGGCGATGTACTGGGCCCAGGCCCTGGCC
GCCCAGGACGAAGACGCCGAGCTGAAAGCGCACTTCGCGCCGCTGGCCAAGGCCCTGACCGAGCAGGAAGCG
ACCATCGTCGCCGAACTCAATGCGGTCCAGGGCAAGCCGGCCGAGATCGGCGGCTACTATCGCTCCAACCCG
GAGCTGACCAGCAAGGTGATGCGCCCCAGCGCCACCTTCAACGCGGCGATCGACAGCCTGGCCTGA
Translation:
MSIRSKITYTFTDEAPALATYSLLPIVKAFAASAGIDVETSDISLAGRILANFADRLDADQRIEDDLARLAV
LATSPDANIIKLPNISASVPQLKGAIAELQGLGYKVPDFPEDPQTDEEKEVRARYAKILGSAVNPVLREGNS
DRRAPAAVKAYARKHPHSMGKWSMASRSHADYMRGGDFFSSEQSITMAKAGDVRIEFVGKDGKVEVKKQLSL
QEGEVLDSMFMSCGKLRDFFEKTLQDCKETGVMWSLHVKATMMKISHPIVFGHAVSVYYKDVFDKWGQLFEE
LGVNPNNGISSVYDKIKSLPASQQEEILHDIHEVYSHRPEMAMVDSVKGITNLHIPSDVIVDASMPAMIRNS
GQMWGKDGKQKDTKAVMPESTYARIYQEMINFCKTNGAFDPTTMGSVPNVGLMAQKAEEYGSHDKTFEMTAD
GTMRVVLADGSVLMQHEVETGDIWRACQTKDAPIRDWVKLAVTRARQSDTPAIFWLDPERAHDRELRKKVEL
YLKDHDLTGLDISIMGYNEAIRVSMERLIRGKDTISVTGNVLRDYLTDLFPIMELGTSAKMLSIVPLMAGGG
MYETGAGGSAPKHVQQLVEENYLRWDSLGEFLALAVSLEETGIKTGNAKAKLLGKALDEATGKLLDNNKSPS
RKVGDIDNRGSHFYLAMYWAQALAAQDEDAELKAHFAPLAKALTEQEATIVAELNAVQGKPAEIGGYYRSNP
ELTSKVMRPSATFNAAIDSLA*
AnnotationID:262GeneID:252
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 11:11:42
Gene Name:idh
Confidence Code:2
GeneProduct:monomeric isocitrate dehydrogenase
Cell Localization:()
Synonyms:icd2
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:isocitrate dehydrogenase [NADP]
Functional Category Confidence Code:5
COGs:COG2838,pfam03971
Secondary Functional Category(ies):central intermediary metabolism
EC Number:1.1.1.42
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD32551.1| (AJ488289) isocitrate dehydrogenase isozyme 2 [Ralstonia eutropha]
          Length = 745

 Score = 1014 bits (2622), Expect = 0.0
 Identities = 505/736 (68%), Positives = 596/736 (80%), Gaps = 2/736 (0%)


>gb|AAK44296.1| (AE006919) isocitrate dehydrogenase, NADP-dependent, monomeric type
           [Mycobacterium tuberculosis CDC1551]
          Length = 745

 Score = 1011 bits (2615), Expect = 0.0
 Identities = 498/736 (67%), Positives = 594/736 (80%), Gaps = 2/736 (0%)

>emb|CAC32204.1| (AL583926) isocitrate dehydrogenase [NADP] [Mycobacterium leprae]
          Length = 746

 Score =  984 bits (2545), Expect = 0.0
 Identities = 488/733 (66%), Positives = 584/733 (79%), Gaps = 2/733 (0%)








 
Structural Features:
COG2838, Icd, Monomeric isocitrate dehydrogenase [Energy production and conversion]
CD-Length = 744 residues,  99.7% aligned
Score = 1314 bits (3403), Expect = 0.0
Identities = 557/742 (75%), Positives = 634/742 (85%), Gaps = 1/742 (0%)


pfam03971, IDH, Monomeric isocitrate dehydrogenase. 
CD-Length = 733 residues, 100.0% aligned
Score = 1315 bits (3405), Expect = 0.0
Identities = 574/733 (78%), Positives = 641/733 (87%), Gaps = 1/733 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:507




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17649




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33191




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:252_cdd.html


    		




 
URL:

















    ReferenceID:34565




  
Author/Investigator(s):
        Fujita Y, Shindo K, Miwa Y, Yoshida K.





  
Title:
        Bacillus subtilis inositol dehydrogenase-encoding gene (idh): sequence and expression in Escherichia coli.




  
PubMed:
        1761221


MedLine:
        




    
Source:
Gene. 1991 Dec 1;108(1):121-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1761221

















    ReferenceID:34566




  
Author/Investigator(s):
        Laporte DC, Stueland CS, Ikeda TP.





  
Title:
        Isocitrate dehydrogenase kinase/phosphatase.




  
PubMed:
        2557093


MedLine:
        




    
Source:
Biochimie. 1989 Sep-Oct;71(9-10):1051-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2557093

















    ReferenceID:34567




  
Author/Investigator(s):
        Wang Y, Lau PC.





  
Title:
        Sequence and expression of an isocitrate dehydrogenase-encoding gene from a polycyclic aromatic hydrocarbon oxidizer, Sphingomonas yanoikuyae B1.




  
PubMed:
        8626059


MedLine:
        




    
Source:
Gene. 1996 Feb 2;168(1):15-21.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8626059

















    ReferenceID:34568




  
Author/Investigator(s):
        Swindell SR, Griffin HG, Gasson MJ.





  
Title:
        Cloning, sequencing and comparison of three lactococcal L-lactate dehydrogenase genes.




  
PubMed:
        8081494


MedLine:
        




    
Source:
Microbiology. 1994 Jun;140 ( Pt 6):1301-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8081494

















    ReferenceID:34569




  
Author/Investigator(s):
        Florio W, Bottai D, Batoni G, Esin S, Pardini M, Maisetta G, Campa M.





  
Title:
        Identification, molecular cloning, and evaluation of potential use of isocitrate dehydrogenase II of Mycobacterium bovis BCG in serodiagnosis of tuberculosis.




  
PubMed:
        12093684


MedLine:
        




    
Source:
Clin Diagn Lab Immunol. 2002 Jul;9(4):846-51.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12093684









           
Homologs By Global Alignment 

           

       



     
Gene ID:252
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
427
                           PA2624_tr
                           translation of PA2624
                           741
                           99.59
                           99.99
                           0
                           3787.0
     

     
     
23349
                           gb|AAG06012.1|AE004691_3
                           (AE004691) isocitrate dehydrogenase [Pseudomonas aeruginosa PAO1]
                           741
                           99.59
                           99.99
                           0
                           3787.0
     

     
     
23351
                           emb|CAD32551.1|
                           (AJ488289) isocitrate dehydrogenase isozyme 2 [Ralstonia eutropha]
                           745
                           68.05
                           80.40
                           4
                           2624.0
     

     
     
23350
                           gb|AAQ61326.1|
                           (AE016923) probable isocitrate dehydrogenase (NADP) [Chromobacterium violaceum ATCC 12472]
                           745
                           67.91
                           80.93
                           4
                           2651.0
     

     
     
23353
                           emb|CAD92929.1|
                           (BX248334) PROBABLE ISOCITRATE DEHYDROGENASE [NADP] ICD2 (OXALOSUCCINATE DECARBOXYLASE) (IDH) (NADP+-SPECIFIC ICDH) (IDP) [Mycobacterium bovis subsp. bovis AF2122/97]
                           745
                           67.11
                           80.00
                           4
                           2614.0
     

     






      

          
GC ORFID: 43254How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2624Glimmer Score: 

          
Start: 2339177Stop: 2341402

          
Length: 2226


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2624 translation of PA2624Homolog Bit Score 1463.0

          
Other Homologs:  t_PA2624 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCATCCGCTCGAAGATCACCTACACCTTCACCGACGAAGCACCCGCGCTCGCCACCTACTCGCTCCTC 
CCCATCGTCAAGGCCTTTGCCGCTTCCGCCGGCATCGACGTCGAGACCAGCGACATCTCTCTTGCAGGACGC 
ATCCTGGCCAACTTCGCCGACCGCCTCGACGCCGACCAGCGTATCGAAGACGACCTCGCCCGCCTGGCGGTC 
CTGGCCACCTCGCCCGACGCCAATATCATCAAGCTGCCCAACATCAGCGCCTCGGTGCCCCAGCTGAAAGGC 
GCAATCGCCGAACTGCAGGGCCTGGGCTACAAGGTTCCCGACTTCCCGGAAGACCCGCAGACCGACGAAGAG 
AAAGAGGTTCGCGCACGCTACGCGAAGATCCTCGGCAGCGCCGTGAACCCGGTCCTGCGCGAAGGCAACTCC 
GACCGTCGCGCGCCTGCCGCGGTGAAGGCCTACGCCCGCAAGCACCCGCACTCCATGGGCAAGTGGAGCATG 
GCCTCCCGCTCCCACGCCGACTACATGCGCGGCGGCGACTTCTTCTCCAGCGAACAGTCCATCACCATGGCC 
AAGGCCGGTGACGTGCGCATCGAGTTCGTCGGCAAGGACGGCAAGGTCGAGGTCAAGAAGCAACTGAGCCTG 
CAGGAAGGCGAAGTGCTCGACAGCATGTTCATGAGCTGCGGCAAGCTGCGCGACTTCTTCGAGAAGACCCTG 
CAGGACTGCAAGGAAACCGGCGTCATGTGGTCCCTGCACGTCAAGGCGACCATGATGAAGATTTCCCACCCG 
ATCGTCTTCGGGCATGCGGTGAGCGTCTACTACAAGGACGTCTTCGACAAGTGGGGCCAACTCTTCGAAGAG 
CTCGGCGTGAACCCGAACAACGGTATCTCCAGCGTCTACGACAAGATCAAGTCGCTGCCCGCCTCCCAGCAG 
GAAGAGATCCTCCACGACATCCACGAGGTGTACAGCCACCGCCCGGAAATGGCGATGGTCGACTCCGTGAAA 
GGCATCACCAACCTGCACATTCCGAGCGACGTCATCGTCGACGCCTCGATGCCGGCGATGATCCGCAACTCC 
GGCCAGATGTGGGGCAAGGACGGCAAGCAGAAAGATACCAAGGCGGTAATGCCGGAAAGCACCTACGCCCGC 
ATCTACCAGGAGATGATCAACTTCTGCAAGACCAACGGCGCCTTCGACCCGACCACCATGGGCAGCGTGCCG 
AACGTCGGCCTGATGGCGCAGAAGGCCGAGGAATACGGCTCCCACGACAAGACCTTCGAAATGACCGCCGAC 
GGCACCATGCGCGTGGTCCTGGCCGACGGCAGCGTGCTGATGCAGCACGAGGTGGAGACAGGCGACATCTGG 
CGCGCCTGCCAGACCAAGGACGCGCCGATCCGCGACTGGGTCAAGCTGGCCGTCACCCGCGCGCGCCAGTCC 
GACACCCCGGCGATCTTCTGGCTGGACCCGGAGCGCGCCCACGACCGCGAACTGCGCAAGAAGGTCGAGCTG 
TACCTCAAGGACCACGACCTGACCGGCCTCGACATCAGCATCATGGGCTACAACGAGGCCATTCGCGTCAGC 
ATGGAACGCCTGATCCGCGGCAAGGACACCATCTCGGTGACCGGCAACGTCCTGCGCGACTACCTGACCGAC 
CTGTTCCCGATCATGGAACTGGGCACCTCGGCCAAGATGCTCTCCATCGTTCCGCTGATGGCGGGCGGCGGC 
ATGTACGAAACCGGCGCCGGCGGCTCGGCACCCAAGCACGTCCAGCAACTGGTGGAAGAGAACTACCTGCGC 
TGGGACTCCCTGGGCGAGTTCCTGGCCCTGGCCGTGTCCCTGGAGGAAACCGGGATCAAGACCGGCAACGCC 
AAGGCCAAGCTGCTCGGCAAGGCGCTGGACGAAGCCACCGGCAAGCTGCTGGACAACAACAAGTCGCCGTCG 
CGCAAGGTCGGCGACATCGACAACCGCGGCAGCCACTTCTACCTGGCGATGTACTGGGCCCAGGCCCTGGCC 
GCCCAGGACGAAGACGCCGAGCTGAAAGCGCACTTCGCGCCGCTGGCCAAGGCCCTGACCGAGCAGGAAGCG 
ACCATCGTCGCCGAACTCAATGCGGTCCAGGGCAAGCCGGCCGAGATCGGCGGCTACTATCGCTCCAACCCG 
GAGCTGACCAGCAAGGTGATGCGCCCCAGCGCCACCTTCAACGCGGCGATCGACAGCCTGGCCTGA