ViewGene
Locus Name:
GeneID:
Location:

GeneID:2512
TrivialName: PA14_16510
AnnotatorUID: danlee
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_16510
PAO1 Ortholog Locus:
Sequence Length:999
Protein Length:332
Start: 1414421
Stop: 1415419
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Proteomics suggests protein starts with "LVEVNRELE" (rather than "MVEVNRELE"). Homology: emb|CAB89696.1| (AJ276418) putative peptide release factor 2 [Xanthomonas campestris pv. campestris]
Identities = 231/330 (70%), Positives = 274/330 (83%)
Sequence:
CTGGTCGAAGTAAACCGCGAGCTCGAAGACCCCAACGTCTGGAACAACCCCGAATACGCCCAGAACCTCGGC
CGCGAGCGCGCCCAGCTGGCGCAGGTGGTGGAAACCCTGGACGACCTGACCAGCGGCCTGGCCGATTCCCGC
GACCTGCTGGACATGGCGGTCGAGGAAGACGACGAGGGCGCCGTCGACGACGTTGCCAACGAAGTCGAGCGT
CTGCGCGAGATCCTCGAGAAGCTGGAGTTCCGCCGCATGTTCAGCGGCGAGATGGACCCGAACAACGCCTAC
CTGGACATCCAGGCCGGCTCCGGCGGCACCGAGGCCCAGGACTGGGCCAACATGCTGCTGCGCATGTACCTG
CGCTGGGCCGACAAGCACGGCTTCGACGCCACTATCATCGAGCTGTCCGAGGGCGAGGTCGCCGGCATCAAG
GGCGCCACCGTGCACATCAAGGGCGAGTACGCGTTCGGCTGGCTGCGCACCGAGATCGGCGTGCACCGCCTG
GTGCGCAAGAGTCCGTTCGACTCCGGCAACCGTCGCCACACCTCGTTCACCGCGGTGTTCGTATCGCCGGAA
ATCGACGACAACATCGAGATCGAGATCAACCCGGCCGACCTGCGCATCGATACCTACCGCTCCTCCGGCGCG
GGCGGTCAGCACGTGAACACCACCGATTCGGCGGTGCGGATCACCCACGTGCCGACCAATACCGTGGTGGCG
TGCCAGAACGAACGCTCCCAGCACGCCAACAAGGACACCGCGATGAAGATGCTGCGGGCCAAGTTGTACGAG
CTGGAAATGCAGAAGCGTACCGCTGCGTCCCAGGCCCTGGAGGACTCCAAGTCCGACATCGGCTGGGGCCAC
CAGATCCGCTCCTACGTGCTCGACCAGTCGCGGATCAAGGACCTGCGTACCGGTATCGAACGCAGCGACTGC
GACAAGGTGCTCGACGGCGATCTAGACGAGTACCTCGAAGCCAGCCTCAAACAGGGGCTGTAA
Translation:
MVEVNRELEDPNVWNNPEYAQNLGRERAQLAQVVETLDDLTSGLADSRDLLDMAVEEDDEGAVDDVANEVER
LREILEKLEFRRMFSGEMDPNNAYLDIQAGSGGTEAQDWANMLLRMYLRWADKHGFDATIIELSEGEVAGIK
GATVHIKGEYAFGWLRTEIGVHRLVRKSPFDSGNRRHTSFTAVFVSPEIDDNIEIEINPADLRIDTYRSSGA
GGQHVNTTDSAVRITHVPTNTVVACQNERSQHANKDTAMKMLRAKLYELEMQKRTAASQALEDSKSDIGWGH
QIRSYVLDQSRIKDLRTGIERSDCDKVLDGDLDEYLEASLKQGL*
AnnotationID:2520GeneID:2512
AnnotatorUID: danlee
Modification Date/Time: 2005-09-26 17:05:43
Gene Name:
Confidence Code:3
GeneProduct:putative peptide chain release factor 2
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1186,COG0216,KOG2726
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAB89696.1| (AJ276418) putative peptide release factor 2 [Xanthomonas
           campestris pv. campestris]
          Length = 374

 Score =  470 bits (1209), Expect = e-132
 Identities = 231/330 (70%), Positives = 274/330 (83%)

>gb|AAC75929.1| (AE000372) peptide chain release factor RF-2 [Escherichia coli K12]
          Length = 365

 Score =  464 bits (1195), Expect = e-130
 Identities = 227/330 (68%), Positives = 264/330 (80%)










 
Structural Features:
COG1186, PrfB, Protein chain release factor B 
[Translation, ribosomal structure and biogenesis]
CD-Length = 239 residues, 100.0% aligned
Score =  346 bits (888), Expect = 5e-96
Identities = 152/239 (63%), Positives = 180/239 (75%), Gaps = 1/239 (0%)

COG0216, PrfA, Protein chain release factor A 
[Translation, ribosomal structure and biogenesis]
CD-Length = 363 residues,  90.9% aligned
Score =  292 bits (749), Expect = 9e-80
Identities = 143/332 (43%), Positives = 196/332 (59%), Gaps = 10/332 (3%)

KOG2726, Mitochondrial polypeptide chain release factor 
[Translation, ribosomal structure and biogenesis]
CD-Length = 386 residues,  80.3% aligned
Score =  265 bits (678), Expect = 1e-71
Identities = 119/310 (38%), Positives = 184/310 (59%), Gaps = 6/310 (1%)






   
Genomic Context:






   
Comment:
Proteomics suggests protein starts with "LVEVNRELE" (rather than "MVEVNRELE").


















    ReferenceID:14611




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22173




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31849




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2512_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2512
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
123918
                           gb|AAM36737.1|
                           (AE011821) peptide chain release factor 2 [Xanthomonas axonopodis pv. citri str. 306]
                           341
                           66.56
                           78.00
                           21
                           1180.0
     

     
     
123919
                           gb|AAF83921.1|AE003947_1
                           (AE003947) peptide chain release factor 2 [Xylella fastidiosa 9a5c]
                           341
                           65.10
                           79.17
                           12
                           1176.0
     

     
     
123926
                           gb|AAM86823.1|AE013928_8
                           (AE013928) peptide chain release factor RF-2 [Yersinia pestis KIM]
                           332
                           64.75
                           75.30
                           22
                           1122.0
     

     
     
123924
                           gb|AAK02274.1|
                           (AE006053) PrfB [Pasteurella multocida]
                           332
                           63.55
                           76.20
                           12
                           1135.0
     

     
     
123925
                           emb|CAE15922.1|
                           (BX571871) Peptide chain release factor 2 (RF-2) [Photorhabdus luminescens subsp. laumondii TTO1]
                           332
                           63.55
                           76.80
                           22
                           1132.0
     

     






      

          
GC ORFID: 45514How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: psyr_15may02_Scaffold3_revised_gene4589Glimmer Score: 

          
Start: 3539516Stop: 3538518

          
Length: 999


          
Start Codon: CTGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: psyr_15may02_Scaffold3_revised_gene4589 related to: gi|7688054|emb|CAB89696.1| (AJ276418) putative peptide release factor 2 [Xanthomonas campestris pv. campestris]Homolog Bit Score 597.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4589 (ORF: GLIMMER)

          
GC ORF Sequence

           
CTGGTCGAAGTAAACCGCGAGCTCGAAGACCCCAACGTCTGGAACAACCCCGAATACGCCCAGAACCTCGGC 
CGCGAGCGCGCCCAGCTGGCGCAGGTGGTGGAAACCCTGGACGACCTGACCAGCGGCCTGGCCGATTCCCGC 
GACCTGCTGGACATGGCGGTCGAGGAAGACGACGAGGGCGCCGTCGACGACGTTGCCAACGAAGTCGAGCGT 
CTGCGCGAGATCCTCGAGAAGCTGGAGTTCCGCCGCATGTTCAGCGGCGAGATGGACCCGAACAACGCCTAC 
CTGGACATCCAGGCCGGCTCCGGCGGCACCGAGGCCCAGGACTGGGCCAACATGCTGCTGCGCATGTACCTG 
CGCTGGGCCGACAAGCACGGCTTCGACGCCACTATCATCGAGCTGTCCGAGGGCGAGGTCGCCGGCATCAAG 
GGCGCCACCGTGCACATCAAGGGCGAGTACGCGTTCGGCTGGCTGCGCACCGAGATCGGCGTGCACCGCCTG 
GTGCGCAAGAGTCCGTTCGACTCCGGCAACCGTCGCCACACCTCGTTCACCGCGGTGTTCGTATCGCCGGAA 
ATCGACGACAACATCGAGATCGAGATCAACCCGGCCGACCTGCGCATCGATACCTACCGCTCCTCCGGCGCG 
GGCGGTCAGCACGTGAACACCACCGATTCGGCGGTGCGGATCACCCACGTGCCGACCAATACCGTGGTGGCG 
TGCCAGAACGAACGCTCCCAGCACGCCAACAAGGACACCGCGATGAAGATGCTGCGGGCCAAGTTGTACGAG 
CTGGAAATGCAGAAGCGTACCGCTGCGTCCCAGGCCCTGGAGGACTCCAAGTCCGACATCGGCTGGGGCCAC 
CAGATCCGCTCCTACGTGCTCGACCAGTCGCGGATCAAGGACCTGCGTACCGGTATCGAACGCAGCGACTGC 
GACAAGGTGCTCGACGGCGATCTAGACGAGTACCTCGAAGCCAGCCTCAAACAGGGGCTGTAA