ViewGene
Locus Name:
GeneID:
Location:

GeneID:2504
TrivialName: PA14_03830
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03830
PAO1 Ortholog Locus:PA0293
Sequence Length:879
Protein Length:292
Start: 344996
Stop: 344118
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03682.1|AE004467_4 (AE004467) N-carbamoylputrescine amidohydrolase [Pseudomonas aeruginosa PAO1]
Identities = 292/292 (100%), Positives = 292/292 (100%)
Sequence:
ATGACCCGCAACGTCACCGTCGCCGCCACCCAGATGGCCTGCTCCTGGGATCGTCCGGCGAACATCGCCCGC
GCCGAGAAACTGGTGCGCCAGGCCGCCGCCAGGGGTGCGCAGATCATCCTGATCCAGGAACTGTTCGAGACC
CCCTACTTCTGCCAGAAGCCGAACCCGGACTACCTGCAGCTGGCCACCACGGTCGAGGAGAACGCCGCCATC
GCCCACTTCCAGGCCCTCGCCCGGGAGTTGCAGGTGGTATTGCCGATCAGCTTCTTCGAACGCGCCGGGCGC
GCCCGCTTCAACAGCATCGCGGTCATCGACGCCGATGGCGGCAACCTCGGGGTCTACCGCAAGAGCCACATC
CCGGACGGCCCGGGCTACCACGAGAAGTACTACTTCAATCCGGGCGACACCGGCTTCAAGGTCTGGCAGACC
CGCTACGCACGGATCGGCGTCGGTATCTGCTGGGACCAGTGGTTCCCCGAATCGGCGCGCAGCATGGCGTTG
CTCGGCGCGGAACTCCTGTTCTACCCGACCGCCATCGGTAGCGAACCGCACGACGCCAGCATCAGCTCGCGC
GATCACTGGCAGCGCGTGCAGCAAGGCCATGCCGGGGCCAACCTGATGCCACTGGTGGCTTCCAACCGCATC
GGCCGCGAGGAGCAGGATGGCTACGACATCACCTTCTATGGTTCCTCGTTCATCGCCGACCCGTTCGGCGAG
AAAGTCGAGGAGCTGAACCGCACCGAGGAAGGTATCCTTGTCCACACCTTCGACCTCGATGCGCTGGAGCGC
ACCCGCAGCGCCTGGGGCGTGTTCCGCGACCGCCGGCCGAACCTCTACGGTCCGCTGAAGACCCTCGACGGT
TCGCTGGAGTCCTGA
Translation:
MTRNVTVAATQMACSWDRPANIARAEKLVRQAAARGAQIILIQELFETPYFCQKPNPDYLQLATTVEENAAI
AHFQALARELQVVLPISFFERAGRARFNSIAVIDADGGNLGVYRKSHIPDGPGYHEKYYFNPGDTGFKVWQT
RYARIGVGICWDQWFPESARSMALLGAELLFYPTAIGSEPHDASISSRDHWQRVQQGHAGANLMPLVASNRI
GREEQDGYDITFYGSSFIADPFGEKVEELNRTEEGILVHTFDLDALERTRSAWGVFRDRRPNLYGPLKTLDG
SLES*
AnnotationID:2512GeneID:2504
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 06:06:10
Gene Name:aguB
Confidence Code:1
GeneProduct:N-carbamoylputrescine amidohydrolase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0388,pfam00795
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58816.1| (AE016875) carbon-nitrogen hydrolase family protein [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 292

 Score =  524 bits (1349), Expect = e-148
 Identities = 250/292 (85%), Positives = 268/292 (91%)

>emb|CAC89781.1| (AJ414145) putative carbon-nitrogen hydrolase [Yersinia pestis
           CO92]
          Length = 294

 Score =  386 bits (991), Expect = e-107
 Identities = 184/287 (64%), Positives = 223/287 (77%), Gaps = 3/287 (1%)

>dbj|BAC60037.1| (AP005079) putative carbon-nitrogen hydrolase [Vibrio
           parahaemolyticus]
          Length = 288

 Score =  348 bits (892), Expect = 3e-95
 Identities = 163/280 (58%), Positives = 201/280 (71%)








 
Structural Features:
COG0388, Predicted amidohydrolase
CD-Length = 274 residues, 100.0% aligned
Score =  215 bits (548), Expect = 6e-57
Identities = 103/287 (35%), Positives = 145/287 (50%), Gaps = 15/287 (5%)

pfam00795, CN_hydrolase, Carbon-nitrogen hydrolase. 
CD-Length = 174 residues, 100.0% aligned
Score =  147 bits (372), Expect = 1e-36
Identities = 63/177 (35%), Positives = 89/177 (50%), Gaps = 6/177 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:5011




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22157




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33644




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2504_cdd.html


    		




 
URL:

















    ReferenceID:35435




  
Author/Investigator(s):
        Nakada Y, Jiang Y, Nishijyo T, Itoh Y, Lu CD.





  
Title:
        Molecular characterization and regulation of the aguBA operon, responsible for agmatine utilization in Pseudomonas aeruginosa PAO1.




  
PubMed:
        11673419


MedLine:
        




    
Source:
J Bacteriol. 2001 Nov;183(22):6517-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11673419









           
Homologs By Global Alignment 

           

       



     
Gene ID:2504
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4186
                           PA0293_tr
                           translation of PA0293
                           292
                           99.99
                           99.99
                           0
                           1543.0
     

     
     
123823
                           gb|AAG03682.1|AE004467_4
                           (AE004467) N-carbamoylputrescine amidohydrolase [Pseudomonas aeruginosa PAO1]
                           292
                           99.99
                           99.99
                           0
                           1543.0
     

     
     
123824
                           gb|AAO58816.1|
                           (AE016875) carbon-nitrogen hydrolase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           292
                           85.61
                           91.78
                           0
                           1349.0
     

     
     
123825
                           gb|AAM86874.1|AE013933_11
                           (AE013933) hypothetical [Yersinia pestis KIM]
                           295
                           63.05
                           76.61
                           4
                           994.0
     

     
     
123826
                           emb|CAC89781.1|
                           (AJ414145) putative carbon-nitrogen hydrolase [Yersinia pestis CO92]
                           295
                           63.05
                           76.61
                           4
                           994.0
     

     






      

          
GC ORFID: 45506How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0293Glimmer Score: 

          
Start: 6146337Stop: 6145459

          
Length: 879


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0293 translation of PA0293Homolog Bit Score 598.0

          
Other Homologs:  psyr_15may02_Scaffold14_revised_gene2438 (ORF: BLASTX 529.0), t_PA0293 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCCGCAACGTCACCGTCGCCGCCACCCAGATGGCCTGCTCCTGGGATCGTCCGGCGAACATCGCCCGC 
GCCGAGAAACTGGTGCGCCAGGCCGCCGCCAGGGGTGCGCAGATCATCCTGATCCAGGAACTGTTCGAGACC 
CCCTACTTCTGCCAGAAGCCGAACCCGGACTACCTGCAGCTGGCCACCACGGTCGAGGAGAACGCCGCCATC 
GCCCACTTCCAGGCCCTCGCCCGGGAGTTGCAGGTGGTATTGCCGATCAGCTTCTTCGAACGCGCCGGGCGC 
GCCCGCTTCAACAGCATCGCGGTCATCGACGCCGATGGCGGCAACCTCGGGGTCTACCGCAAGAGCCACATC 
CCGGACGGCCCGGGCTACCACGAGAAGTACTACTTCAATCCGGGCGACACCGGCTTCAAGGTCTGGCAGACC 
CGCTACGCACGGATCGGCGTCGGTATCTGCTGGGACCAGTGGTTCCCCGAATCGGCGCGCAGCATGGCGTTG 
CTCGGCGCGGAACTCCTGTTCTACCCGACCGCCATCGGTAGCGAACCGCACGACGCCAGCATCAGCTCGCGC 
GATCACTGGCAGCGCGTGCAGCAAGGCCATGCCGGGGCCAACCTGATGCCACTGGTGGCTTCCAACCGCATC 
GGCCGCGAGGAGCAGGATGGCTACGACATCACCTTCTATGGTTCCTCGTTCATCGCCGACCCGTTCGGCGAG 
AAAGTCGAGGAGCTGAACCGCACCGAGGAAGGTATCCTTGTCCACACCTTCGACCTCGATGCGCTGGAGCGC 
ACCCGCAGCGCCTGGGGCGTGTTCCGCGACCGCCGGCCGAACCTCTACGGTCCGCTGAAGACCCTCGACGGT 
TCGCTGGAGTCCTGA