ViewGene
Locus Name:
GeneID:
Location:

GeneID:2503
TrivialName: PA14_00120
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_00120
PAO1 Ortholog Locus:PA0011
Sequence Length:888
Protein Length:295
Start: 14236
Stop: 15123
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03401.1|AE004441_2 (AE004441) probable 2-OH-lauroyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 295/295 (100%), Positives = 295/295 (100%)
Sequence:
GTGGAGAAATTCAAAGGTGCCCTGGTGGTGGGGGCCCTACGCCTGTTCGCGCTATTGCCCTGGCGTGCCGTG
CAAGGCGTCGGGGCCGGCATAGGCTGGCTGATGTGGAAATTGCCGAATCGATCCCGCGAGGTAGTGCGGATC
AACCTGTCCAAGTGCTTCCCCGAGTTGTCCGAAACCGAACTGGAAAAGCTGGTCGGACAAAGCCTGATGGAT
ATTGGCCGTACCCTCACCGAAAGTGCCTGTGCCTGGATCTGGCCGCCTGAAAAGTCGCTGAGGTACATCCGC
GAGGTCGAAGGCATGGAAGTGCTGGAGGAAGCGCTGGCTTCCGGCGATGGCCTGGTCGGCATTACCAGTCAC
CTGGGAAACTGGGAAGTACTCAACCACTTCTATTGCTCTTACGCCAAGCCGATCATCTTCTATCGTCCGCCC
AAGCTGAAGGCAGTGGACGAGTTGCTGAAGAAGCAACGCGTGCAATTGGGCAATCGCGTCGCACCTTCCACT
CCGGAGGGTATCCTCAGTGTCATCAAGGAAGTGAAGAAAGGCGGTTGCGTAGGGATTCCCGCCGACCCCGAG
CCCGCGCGTACCGCTGGGCTCTTCGTGCCCTACCTGGGCACCACTGCATTGATCAGCAAGTTCGTCCCGCAG
TTGCTTTCACGCGGCAAGGCGCGTGGAGTGTTCTTCCATGCGGTGCGCCTGCCCGATGGTAGCGGTTACAAG
GTGATCCTCGAAGCGGCTCCGGCGGACATGTACGACAAGGACCTGGAAGTGTCTGTAGCAGCCATGAGCCGC
GAGTTGGCGAAGTATGTACGAGCCTATCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAACCGCCCG
GATGGCGAGAAAAAATGGTACTGA
Translation:
MEKFKGALVVGALRLFALLPWRAVQGVGAGIGWLMWKLPNRSREVVRINLSKCFPELSETELEKLVGQSLMD
IGRTLTESACAWIWPPEKSLRYIREVEGMEVLEEALASGDGLVGITSHLGNWEVLNHFYCSYAKPIIFYRPP
KLKAVDELLKKQRVQLGNRVAPSTPEGILSVIKEVKKGGCVGIPADPEPARTAGLFVPYLGTTALISKFVPQ
LLSRGKARGVFFHAVRLPDGSGYKVILEAAPADMYDKDLEVSVAAMSRELAKYVRAYPSQYMWSMKRFKNRP
DGEKKWY*
AnnotationID:2511GeneID:2503
AnnotatorUID: diggins
Modification Date/Time: 2005-03-22 11:11:38
Gene Name:
Confidence Code:3
GeneProduct:putative 2-OH-lauroyltransferase
Cell Localization:()
Synonyms:PA0011
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:putative lipid A biosynthesis lauroyl acyltransferase
Functional Category Confidence Code:5
COGs:COG1560,pfam03279
Secondary Functional Category(ies):fatty acid and phospholipid metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53736.1| (AE016856) lipid A biosynthesis lauroyl acyltransferase, putative
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 295

 Score =  488 bits (1256), Expect = e-137
 Identities = 234/295 (79%), Positives = 261/295 (88%)

>gb|AAN65697.1|AE016196_8 (AE016774) lipid A biosynthesis lauroyl acyltransferase, putative
           [Pseudomonas putida KT2440]
          Length = 295

 Score =  462 bits (1190), Expect = e-130
 Identities = 218/295 (73%), Positives = 253/295 (85%)

>emb|CAA74897.1| (Y14568) htrB [Pseudomonas fluorescens]
          Length = 253

 Score =  409 bits (1050), Expect = e-113
 Identities = 197/251 (78%), Positives = 222/251 (88%)






 
Structural Features:
COG1560, HtrB, Lauroyl/myristoyl acyltransferase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 308 residues,  95.8% aligned
Score =  290 bits (745), Expect = 1e-79
Identities = 117/296 (39%), Positives = 163/296 (55%), Gaps = 2/296 (0%)

pfam03279, Lip_A_acyltrans, Bacterial lipid A biosynthesis acyltransferase.
CD-Length = 295 residues,  93.9% aligned
Score =  159 bits (404), Expect = 4e-40
Identities = 89/279 (31%), Positives = 142/279 (50%), Gaps = 3/279 (1%)









   
Genomic Context:






   
Comment:


















    ReferenceID:14609




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22155




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33380




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2503_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2503
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4185
                           PA0011_tr
                           translation of PA0011
                           295
                           99.99
                           99.99
                           0
                           1543.0
     

     
     
123819
                           gb|AAG03401.1|AE004441_2
                           (AE004441) probable 2-OH-lauroyltransferase [Pseudomonas aeruginosa PAO1]
                           295
                           99.66
                           99.99
                           0
                           1540.0
     

     
     
123820
                           gb|AAO53736.1|
                           (AE016856) lipid A biosynthesis lauroyl acyltransferase, putative [Pseudomonas syringae pv. tomato str. DC3000]
                           295
                           78.98
                           88.47
                           0
                           1252.0
     

     
     
123821
                           gb|AAN65697.1|AE016196_8
                           (AE016774) lipid A biosynthesis lauroyl acyltransferase, putative [Pseudomonas putida KT2440]
                           295
                           73.55
                           85.76
                           0
                           1186.0
     

     
     
123822
                           emb|CAA74897.1|
                           (Y14568) htrB [Pseudomonas fluorescens]
                           295
                           66.44
                           75.25
                           42
                           1045.0
     

     






      

          
GC ORFID: 45505How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0011Glimmer Score: 

          
Start: 5815573Stop: 5816460

          
Length: 888


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0011 translation of PA0011Homolog Bit Score 598.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene2973 (ORF: BLASTX 485.0), t_PA0011 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGGAGAAATTCAAAGGTGCCCTGGTGGTGGGGGCCCTACGCCTGTTCGCGCTATTGCCCTGGCGTGCCGTG 
CAAGGCGTCGGGGCCGGCATAGGCTGGCTGATGTGGAAATTGCCGAATCGATCCCGCGAGGTAGTGCGGATC 
AACCTGTCCAAGTGCTTCCCCGAGTTGTCCGAAACCGAACTGGAAAAGCTGGTCGGACAAAGCCTGATGGAT 
ATTGGCCGTACCCTCACCGAAAGTGCCTGTGCCTGGATCTGGCCGCCTGAAAAGTCGCTGAGGTACATCCGC 
GAGGTCGAAGGCATGGAAGTGCTGGAGGAAGCGCTGGCTTCCGGCGATGGCCTGGTCGGCATTACCAGTCAC 
CTGGGAAACTGGGAAGTACTCAACCACTTCTATTGCTCTTACGCCAAGCCGATCATCTTCTATCGTCCGCCC 
AAGCTGAAGGCAGTGGACGAGTTGCTGAAGAAGCAACGCGTGCAATTGGGCAATCGCGTCGCACCTTCCACT 
CCGGAGGGTATCCTCAGTGTCATCAAGGAAGTGAAGAAAGGCGGTTGCGTAGGGATTCCCGCCGACCCCGAG 
CCCGCGCGTACCGCTGGGCTCTTCGTGCCCTACCTGGGCACCACTGCATTGATCAGCAAGTTCGTCCCGCAG 
TTGCTTTCACGCGGCAAGGCGCGTGGAGTGTTCTTCCATGCGGTGCGCCTGCCCGATGGTAGCGGTTACAAG 
GTGATCCTCGAAGCGGCTCCGGCGGACATGTACGACAAGGACCTGGAAGTGTCTGTAGCAGCCATGAGCCGC 
GAGTTGGCGAAGTATGTACGAGCCTATCCCAGCCAGTACATGTGGAGCATGAAGCGCTTCAAGAACCGCCCG 
GATGGCGAGAAAAAATGGTACTGA