ViewGene
Locus Name:
GeneID:
Location:

GeneID:2497
TrivialName: PA14_56740
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_56740
PAO1 Ortholog Locus:PA4363
Sequence Length:903
Protein Length:300
Start: 5055799
Stop: 5054897
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07751.1|AE004852_4 (AE004852) inhibitor of chromosome initiation IciA [Pseudomonas aeruginosa PAO1]
Identities = 300/300 (100%), Positives = 300/300 (100%)
Sequence:
CTGATTTTGTTCGACTACAAGTTGCTCGCCGCGCTGGCCGCGGTGGTGGAGCAGGGCGGTTTCGAGCGCGCC
GCACAGGCGCTGGGGCTGTCGCAATCGGCGGTATCGCAGCGGATCAAGCTGCTCGAGGCGCGGGTCGGCCAG
CCGGTCCTGGTGCGCGAGACGCCGCCGCATCCCACCGATCTCGGGCGCCGCCTGCTCAACCATGTGCAGCAG
GTGCGGCTGCTGGAGGGCGACCTGCAGCGCTGGGTGCCGAACCTGGATGAAGGCGGTGCCCCGGAGCGCCTG
CGCATCGCTCTCAACGCCGATAGCCTGGCGACCTGGTGGGCCGCCGCGGTGGGCGATTTCTGCGCCGAGCGG
CGGGTGCTGCTGGACCTTGTGGTGGAGGACCAGGAGGTCGGGCTCAAGCGCATGCGCGCGGGCGAAGTGGCG
GGCTGCGTGTGCGGTAGCGCGCGGCCGGTGGCCGGCGCGCGTTCGCTGCTGCTCGGAGCCATGCGTTATCGC
GGCCTGGCCAGCCCGGATTTCATTGCCCGGCACTTTCCCCGCGGGGTCGAGGCGGCCGCCCTGGCCGGCGTG
CCGGCGATCGTGTTCGGCCCGGACGACCTGCTGCAACACCGCTTTCTCAAGGACCTCGGCGTCGAGGGCGGT
TTCATCCACCACCTCTGCCCATCCTCGGAAGGTTTCGTCCGCCTCACCGCCGGCGGTCTCGGCTGGGGCCTG
GTGCCGGAGCGCCAGGTGCAAGGCGAGCTGGCGCGCGGCGAACTGGTCGAGCTGCTGCCCGGCCAGGTCATC
GACGTACCGTTGTACTGGCACTACTGGCGCAACGGCGGCGAACTGCTCGCGTCGCTCACCGAGCACCTGCTC
GCCCGCGCCGGCGACGGCTTGGTGCGGGTGTCAGGTTGA
Translation:
MILFDYKLLAALAAVVEQGGFERAAQALGLSQSAVSQRIKLLEARVGQPVLVRETPPHPTDLGRRLLNHVQQ
VRLLEGDLQRWVPNLDEGGAPERLRIALNADSLATWWAAAVGDFCAERRVLLDLVVEDQEVGLKRMRAGEVA
GCVCGSARPVAGARSLLLGAMRYRGLASPDFIARHFPRGVEAAALAGVPAIVFGPDDLLQHRFLKDLGVEGG
FIHHLCPSSEGFVRLTAGGLGWGLVPERQVQGELARGELVELLPGQVIDVPLYWHYWRNGGELLASLTEHLL
ARAGDGLVRVSG*
AnnotationID:2505GeneID:2497
AnnotatorUID: diggins
Modification Date/Time: 2005-05-20 13:01:12
Gene Name:iciA
Confidence Code:2
GeneProduct:inhibitor of chromosome initiation
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:transcriptional regulator
Functional Category Confidence Code:5
COGs:COG0583,pfam00126
Secondary Functional Category(ies):transcriptional regulator
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66542.1|AE016283_1 (AE016777) transcriptional regulator IciA, putative [Pseudomonas
           putida KT2440]
          Length = 296

 Score =  452 bits (1162), Expect = e-126
 Identities = 224/294 (76%), Positives = 251/294 (85%)

>gb|AAO57811.1| (AE016871) chromosome initiation inhibitor [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 299

 Score =  439 bits (1128), Expect = e-122
 Identities = 217/297 (73%), Positives = 242/297 (81%)








 
Structural Features:
COG0583, LysR, Transcriptional regulator [Transcription]
CD-Length = 297 residues,  94.3% aligned
Score = 88.5 bits (218), Expect = 2e-18

pfam00126, HTH_1, Bacterial regulatory helix-turn-helix protein, 
lysR family.
CD-Length = 60 residues, 100.0% aligned
Score = 59.4 bits (144), Expect = 1e-09







   
Genomic Context:






   
Comment:


















    ReferenceID:14607




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17114




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2497_cdd.html


    		




 
URL:

















    ReferenceID:22143




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38153




  
Author/Investigator(s):
        Thony B, Hwang DS, Fradkin L, Kornberg A.





  
Title:
        iciA, an Escherichia coli gene encoding a specific inhibitor of chromosomal initiation of replication in vitro.




  
PubMed:
        2034653


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1991 May 15;88(10):4066-70.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2034653

















    ReferenceID:38154




  
Author/Investigator(s):
        Hwang DS, Thony B, Kornberg A.





  
Title:
        IciA protein, a specific inhibitor of initiation of Escherichia coli chromosomal replication.




  
PubMed:
        1733927


MedLine:
        




    
Source:
J Biol Chem. 1992 Feb 5;267(4):2209-13.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1733927&query_hl=12









           
Homologs By Global Alignment 

           

       



     
Gene ID:2497
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4178
                           PA4363_tr
                           translation of PA4363
                           300
                           99.66
                           99.99
                           0
                           1544.0
     

     
     
123759
                           gb|AAG07751.1|AE004852_4
                           (AE004852) inhibitor of chromosome initiation IciA [Pseudomonas aeruginosa PAO1]
                           300
                           99.66
                           99.99
                           0
                           1544.0
     

     
     
123760
                           gb|AAN66542.1|AE016283_1
                           (AE016777) transcriptional regulator IciA, putative [Pseudomonas putida KT2440]
                           300
                           74.66
                           84.00
                           4
                           1161.0
     

     
     
123761
                           gb|AAO57811.1|
                           (AE016871) chromosome initiation inhibitor [Pseudomonas syringae pv. tomato str. DC3000]
                           301
                           72.09
                           80.39
                           3
                           1126.0
     

     
     
123762
                           gb|AAQ60239.1|
                           (AE016919) inhibitor of chromosome initiation IciA [Chromobacterium violaceum ATCC 12472]
                           323
                           44.89
                           59.44
                           34
                           636.0
     

     






      

          
GC ORFID: 45499How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4363Glimmer Score: 

          
Start: 4319475Stop: 4318573

          
Length: 903


          
Start Codon: CTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4363 translation of PA4363Homolog Bit Score 599.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene2066 (ORF: BLASTX 442.0), t_PA4363 (ORF: GLIMMER)

          
GC ORF Sequence

           
CTGATTTTGTTCGACTACAAGTTGCTCGCCGCGCTGGCCGCGGTGGTGGAGCAGGGCGGTTTCGAGCGCGCC 
GCACAGGCGCTGGGGCTGTCGCAATCGGCGGTATCGCAGCGGATCAAGCTGCTCGAGGCGCGGGTCGGCCAG 
CCGGTCCTGGTGCGCGAGACGCCGCCGCATCCCACCGATCTCGGGCGCCGCCTGCTCAACCATGTGCAGCAG 
GTGCGGCTGCTGGAGGGCGACCTGCAGCGCTGGGTGCCGAACCTGGATGAAGGCGGTGCCCCGGAGCGCCTG 
CGCATCGCTCTCAACGCCGATAGCCTGGCGACCTGGTGGGCCGCCGCGGTGGGCGATTTCTGCGCCGAGCGG 
CGGGTGCTGCTGGACCTTGTGGTGGAGGACCAGGAGGTCGGGCTCAAGCGCATGCGCGCGGGCGAAGTGGCG 
GGCTGCGTGTGCGGTAGCGCGCGGCCGGTGGCCGGCGCGCGTTCGCTGCTGCTCGGAGCCATGCGTTATCGC 
GGCCTGGCCAGCCCGGATTTCATTGCCCGGCACTTTCCCCGCGGGGTCGAGGCGGCCGCCCTGGCCGGCGTG 
CCGGCGATCGTGTTCGGCCCGGACGACCTGCTGCAACACCGCTTTCTCAAGGACCTCGGCGTCGAGGGCGGT 
TTCATCCACCACCTCTGCCCATCCTCGGAAGGTTTCGTCCGCCTCACCGCCGGCGGTCTCGGCTGGGGCCTG 
GTGCCGGAGCGCCAGGTGCAAGGCGAGCTGGCGCGCGGCGAACTGGTCGAGCTGCTGCCCGGCCAGGTCATC 
GACGTACCGTTGTACTGGCACTACTGGCGCAACGGCGGCGAACTGCTCGCGTCGCTCACCGAGCACCTGCTC 
GCCCGCGCCGGCGACGGCTTGGTGCGGGTGTCAGGTTGA