ViewGene
Locus Name:
GeneID:
Location:

GeneID:2494
TrivialName: PA14_20390
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20390
PAO1 Ortholog Locus:PA3377
Sequence Length:885
Protein Length:294
Start: 1753981
Stop: 1754865
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06765.1|AE004759_12 (AE004759) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 293/294 (99%), Positives = 294/294 (100%)
Sequence:
ATGAGCCTTTCCCCGCAAGCCAGCGCCGCGCCGGAGCAGGGCTACAACTTCGCCTACCTCGACGAGCAGACC
AAGCGCATGATCCGCCGCGGCCTGCTCAAGGCGGTGGCGATCCCCGGCTACCAGGTGCCCTTCGGCGGCCGC
GAGATGCCGCTGCCCTACGGCTGGGGCACCGGCGGCATGCAGGTGACCGCGGCCATCCTCGGTGCCGACGAC
GTGCTCAAGGTGATCGACCAGGGCGCCGACGACACCACCAACGCGGTATCCATCCGGCGTTTCTTCGCCCGC
ACCGCCGGGGTCGCCACCACCGAACGGACCCCGGAGGCGACGGTGATCCAGACCCGCCACCGGATACCGGAA
ACCGCCCTGAGCGGCGAGCAGATCATGGTCTACCAGGTGCCGATCCCCGAGCCGCTGCGCTTCATCGAGCCT
TCCGAGGCGGAGACCCGGACCATGCATGCGCTCAACGACTACGGGGTGATGCACGTGAAGCTCTACGAGGAC
ATCGCCACCTTCGGCCACATCGCCACCAGCTATGCCTACCCGGTGACGGTGGACGAGCGCTACGTGATGGAT
CCCTCGCCGATTCCCAAGTTCGACAACCCCAAGCTGGACATGAACCCGGCGCTGATGCTGTTCGGCGCCGGT
CGCGAGAAGCGCCTGTACGCGGTGCCGCCGTTCACCCGGGTGGCGAGCCTGGACTTCGAGGACCACCCCTTC
GAGGTCCAGCGCTGGGAGGAAAGCTGCGCCTTCTGCGGCAGCCACGAGTCCTACCTGGACGAACTGATCGTC
GACGACCGTGGCGGCAAGCGCTTCGTCTGTTCCGACACCGATTACTGCACGCAGCGGCGTGCCCGCCAGGAA
GAAGGGGAGGACGCGCAATGA
Translation:
MSLSPQASAAPEQGYNFAYLDEQTKRMIRRGLLKAVAIPGYQVPFGGREMPLPYGWGTGGMQVTAAILGADD
VLKVIDQGADDTTNAVSIRRFFARTAGVATTERTPEATVIQTRHRIPETALSGEQIMVYQVPIPEPLRFIEP
SEAETRTMHALNDYGVMHVKLYEDIATFGHIATSYAYPVTVDERYVMDPSPIPKFDNPKLDMNPALMLFGAG
REKRLYAVPPFTRVASLDFEDHPFEVQRWEESCAFCGSHESYLDELIVDDRGGKRFVCSDTDYCTQRRARQE
EGEDAQ*
AnnotationID:2502GeneID:2494
AnnotatorUID: danlee
Modification Date/Time: 2005-07-29 14:02:27
Gene Name:phnJ
Confidence Code:3
GeneProduct:putative phosphonate metabolism protein
Cell Localization:()
Synonyms:PA3377
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam06007,COG3627
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56067.1| (AE016865) phosphonate metabolism protein PhnJ [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 295

 Score =  508 bits (1307), Expect = e-143
 Identities = 245/283 (86%), Positives = 260/283 (91%)

>gb|AAN83528.1|AE016771_39 (AE016771) PhnJ protein [Escherichia coli CFT073]
          Length = 309

 Score =  456 bits (1172), Expect = e-128
 Identities = 212/276 (76%), Positives = 241/276 (87%)

>emb|CAC49854.1| (AL603647) putative C-P (carbon-phosphorus) lyase component protein
           [Sinorhizobium meliloti]
          Length = 297

 Score =  436 bits (1120), Expect = e-121
 Identities = 205/276 (74%), Positives = 233/276 (84%)








 
Structural Features:
pfam06007, PhnJ, Phosphonate metabolism protein PhnJ.
CD-Length = 279 residues, 100.0% aligned
Score =  523 bits (1349), Expect = 7e-150
Identities = 221/279 (79%), Positives = 248/279 (88%)

COG3627, PhnJ, Uncharacterized enzyme of phosphonate metabolism 
[Inorganic ion transport and metabolism]
CD-Length = 291 residues, 100.0% aligned
Score =  534 bits (1378), Expect = 3e-153
Identities = 226/291 (77%), Positives = 252/291 (86%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4991




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22137




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32185




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2494_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2494
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4175
                           PA3377_tr
                           translation of PA3377
                           294
                           99.65
                           99.99
                           0
                           1545.0
     

     
     
123713
                           gb|AAG06765.1|AE004759_12
                           (AE004759) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           294
                           99.65
                           99.99
                           0
                           1545.0
     

     
     
123714
                           gb|AAO56067.1|
                           (AE016865) phosphonate metabolism protein PhnJ [Pseudomonas syringae pv. tomato str. DC3000]
                           298
                           82.21
                           87.91
                           7
                           1306.0
     

     
     
123718
                           dbj|BAB38504.1|
                           (AP002568) phosphonate metabolism [Escherichia coli O157:H7]
                           294
                           73.12
                           82.65
                           13
                           1177.0
     

     
     
123717
                           dbj|BAA14270.1|
                           (D90227) phnJ [Escherichia coli]
                           294
                           73.12
                           82.65
                           13
                           1177.0
     

     






      

          
GC ORFID: 45496How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3377Glimmer Score: 

          
Start: 3199951Stop: 3199067

          
Length: 885


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3377 translation of PA3377Homolog Bit Score 599.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1223 (ORF: BLASTX 514.0), t_PA3377 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCTTTCCCCGCAAGCCAGCGCCGCGCCGGAGCAGGGCTACAACTTCGCCTACCTCGACGAGCAGACC 
AAGCGCATGATCCGCCGCGGCCTGCTCAAGGCGGTGGCGATCCCCGGCTACCAGGTGCCCTTCGGCGGCCGC 
GAGATGCCGCTGCCCTACGGCTGGGGCACCGGCGGCATGCAGGTGACCGCGGCCATCCTCGGTGCCGACGAC 
GTGCTCAAGGTGATCGACCAGGGCGCCGACGACACCACCAACGCGGTATCCATCCGGCGTTTCTTCGCCCGC 
ACCGCCGGGGTCGCCACCACCGAACGGACCCCGGAGGCGACGGTGATCCAGACCCGCCACCGGATACCGGAA 
ACCGCCCTGAGCGGCGAGCAGATCATGGTCTACCAGGTGCCGATCCCCGAGCCGCTGCGCTTCATCGAGCCT 
TCCGAGGCGGAGACCCGGACCATGCATGCGCTCAACGACTACGGGGTGATGCACGTGAAGCTCTACGAGGAC 
ATCGCCACCTTCGGCCACATCGCCACCAGCTATGCCTACCCGGTGACGGTGGACGAGCGCTACGTGATGGAT 
CCCTCGCCGATTCCCAAGTTCGACAACCCCAAGCTGGACATGAACCCGGCGCTGATGCTGTTCGGCGCCGGT 
CGCGAGAAGCGCCTGTACGCGGTGCCGCCGTTCACCCGGGTGGCGAGCCTGGACTTCGAGGACCACCCCTTC 
GAGGTCCAGCGCTGGGAGGAAAGCTGCGCCTTCTGCGGCAGCCACGAGTCCTACCTGGACGAACTGATCGTC 
GACGACCGTGGCGGCAAGCGCTTCGTCTGTTCCGACACCGATTACTGCACGCAGCGGCGTGCCCGCCAGGAA 
GAAGGGGAGGACGCGCAATGA