ViewGene
Locus Name:
GeneID:
Location:

GeneID:2493
TrivialName: PA14_25650
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25650
PAO1 Ortholog Locus:PA2968
Sequence Length:939
Protein Length:312
Start: 2242261
Stop: 2243199
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06356.1|AE004722_12 (AE004722) malonyl-CoA-[acyl-carrier-protein] transacylase [Pseudomonas aeruginosa PAO1]
Identities = 311/312 (99%), Positives = 312/312 (100%)
Sequence:
ATGTCTGCATCCCTCGCATTCGTCTTCCCCGGCCAGGGTTCGCAATCCCTCGGCATGCTGGCCGAGCTGGGC
GCCCAGCAGGCGCTGGTGCGCGATACCTTCGCCGAGGCCTCCGAGGCGCTCGGTTACGACCTTTGGGCGCTG
GTCCAGAATGGACCTGAAGAGCGCCTGAACCAGACCGACAAGACCCAGCCGGCCATCCTCACGGTTTCGATC
GCGCTCTGGCGCCTCTGGCTGGCCGAGGGCGGCGCGCGCCCGGCGTTCGTCGCCGGGCACAGCTTGGGCGAA
TATTCCGCGCTGGTCGCGGCCGAAAGCCTGGCGTTCGCCGATGCGGTCAAGCTGGTCGAGCGTAGGGGCCAA
CTGATGCAGCAGGCGGTTCCGGCGGGGCAGGGCGGCATGGCCGCGATCCTAGGCCTGGAAGACGCCGATGTA
TTGGCGGCCTGTGCCGAGGCGGCCCAGGGCGAGGTGGTCAGCGCGGTCAACTTCAACGCGCCGGGGCAGGTA
GTGATCGCCGGTGCCGCGGCTGCCGTGGAGCGTGCCATCGAGGCGTGCAAGGCGCGCGGCGCCAAGCGCGCG
GTGGCGCTGCCGGTCAGCGTGCCGTCGCATTGCGAACTGATGCGTCCGGCCGCCGAGCAGTTCGCCGCCTCG
GTCGAAAGCTTGCAGTGGCAGGCGCCGAAGATTGCGCTGGTGCAGAACGTCAGCGCCGCCGTGCCGGCTGAT
CTCGATACGCTGCGCCGCGACCTGCTGGCACAGCTGTACAGCCCGGTTCGCTGGGTGGAGAGCATCCAGCTG
CTGGCGGAAAAGGGCGTCACCGAGCTGGTCGAGTGCGGGCCGGGCAAGGTCCTGGCAGGCCTCAACAGGCGC
TGTGCGAAGGGCATCAATACCCATGGCCTGGATGGTGTCGAGGCGTTCGCCGCCACGCGCGCCGCCCTGGCC
TGA
Translation:
MSASLAFVFPGQGSQSLGMLAELGAQQALVRDTFAEASEALGYDLWALVQNGPEERLNQTDKTQPAILTVSI
ALWRLWLAEGGARPAFVAGHSLGEYSALVAAESLAFADAVKLVERRGQLMQQAVPAGQGGMAAILGLEDADV
LAACAEAAQGEVVSAVNFNAPGQVVIAGAAAAVERAIEACKARGAKRAVALPVSVPSHCELMRPAAEQFAAS
VESLQWQAPKIALVQNVSAAVPADLDTLRRDLLAQLYSPVRWVESIQLLAEKGVTELVECGPGKVLAGLNRR
CAKGINTHGLDGVEAFAATRAALA*
AnnotationID:2501GeneID:2493
AnnotatorUID: diggins
Modification Date/Time: 2005-04-11 07:07:25
Gene Name:fabD
Confidence Code:1
GeneProduct:malonyl-CoA-[acyl-carrier-protein] transacylase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:(acyl-carrier-protein) S-malonyltransferase
Functional Category Confidence Code:5
COGs:COG0331
Secondary Functional Category(ies):
EC Number:2.3.1.39
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57301.1| (AE016869) malonyl CoA-acyl carrier protein transacylase
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 312

 Score =  497 bits (1279), Expect = e-140
 Identities = 252/312 (80%), Positives = 279/312 (89%)

>gb|AAN67531.1|AE016381_8 (AE016781) malonyl CoA-acyl carrier protein transacylase
           [Pseudomonas putida KT2440]
          Length = 312

 Score =  466 bits (1199), Expect = e-131
 Identities = 234/312 (75%), Positives = 271/312 (86%)

>gb|AAN55802.1|AE015717_3 (AE015717) malonyl CoA-acyl carrier protein transacylase
           [Shewanella oneidensis MR-1]
          Length = 308

 Score =  381 bits (979), Expect = e-105
 Identities = 191/303 (63%), Positives = 235/303 (77%)








 
Structural Features:
COG0331, FabD, (acyl-carrier-protein) S-malonyltransferase [Lipid metabolism]
CD-Length = 310 residues, 100.0% aligned
Score =  336 bits (862), Expect = 3e-93
Identities = 163/310 (52%), Positives = 209/310 (67%), Gaps = 1/310 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4989




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22135




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36082




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2493_cdd.html


    		




 
URL:

















    ReferenceID:36442




  
Author/Investigator(s):
        Kutchma AJ, Hoang TT, Schweizer HP.





  
Title:
        Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD).




  
PubMed:
        10464226


MedLine:
        




    
Source:
J Bacteriol. 1999 Sep;181(17):5498-504.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10464226









           
Homologs By Global Alignment 

           

       



     
Gene ID:2493
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4174
                           PA2968_tr
                           translation of PA2968
                           312
                           99.67
                           99.99
                           0
                           1545.0
     

     
     
123620
                           gb|AAG06356.1|AE004722_12
                           (AE004722) malonyl-CoA-[acyl-carrier-protein] transacylase [Pseudomonas aeruginosa PAO1]
                           312
                           99.67
                           99.99
                           0
                           1545.0
     

     
     
123621
                           gb|AAB94394.1|
                           (U91631) malonyl-CoA:acyl carrier protein transacylase [Pseudomonas aeruginosa]
                           312
                           99.67
                           99.99
                           0
                           1545.0
     

     
     
123622
                           gb|AAO57301.1|
                           (AE016869) malonyl CoA-acyl carrier protein transacylase [Pseudomonas syringae pv. tomato str. DC3000]
                           312
                           80.76
                           89.42
                           0
                           1279.0
     

     
     
123623
                           gb|AAN67531.1|AE016381_8
                           (AE016781) malonyl CoA-acyl carrier protein transacylase [Pseudomonas putida KT2440]
                           312
                           75.00
                           86.85
                           0
                           1199.0
     

     






      

          
GC ORFID: 45495How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2968Glimmer Score: 

          
Start: 2711664Stop: 2710726

          
Length: 939


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2968 translation of PA2968Homolog Bit Score 599.0

          
Other Homologs:  psyr_15may02_Scaffold13_revised_gene2161 (ORF: BLASTX 500.0), t_PA2968 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCTGCATCCCTCGCATTCGTCTTCCCCGGCCAGGGTTCGCAATCCCTCGGCATGCTGGCCGAGCTGGGC 
GCCCAGCAGGCGCTGGTGCGCGATACCTTCGCCGAGGCCTCCGAGGCGCTCGGTTACGACCTTTGGGCGCTG 
GTCCAGAATGGACCTGAAGAGCGCCTGAACCAGACCGACAAGACCCAGCCGGCCATCCTCACGGTTTCGATC 
GCGCTCTGGCGCCTCTGGCTGGCCGAGGGCGGCGCGCGCCCGGCGTTCGTCGCCGGGCACAGCTTGGGCGAA 
TATTCCGCGCTGGTCGCGGCCGAAAGCCTGGCGTTCGCCGATGCGGTCAAGCTGGTCGAGCGTAGGGGCCAA 
CTGATGCAGCAGGCGGTTCCGGCGGGGCAGGGCGGCATGGCCGCGATCCTAGGCCTGGAAGACGCCGATGTA 
TTGGCGGCCTGTGCCGAGGCGGCCCAGGGCGAGGTGGTCAGCGCGGTCAACTTCAACGCGCCGGGGCAGGTA 
GTGATCGCCGGTGCCGCGGCTGCCGTGGAGCGTGCCATCGAGGCGTGCAAGGCGCGCGGCGCCAAGCGCGCG 
GTGGCGCTGCCGGTCAGCGTGCCGTCGCATTGCGAACTGATGCGTCCGGCCGCCGAGCAGTTCGCCGCCTCG 
GTCGAAAGCTTGCAGTGGCAGGCGCCGAAGATTGCGCTGGTGCAGAACGTCAGCGCCGCCGTGCCGGCTGAT 
CTCGATACGCTGCGCCGCGACCTGCTGGCACAGCTGTACAGCCCGGTTCGCTGGGTGGAGAGCATCCAGCTG 
CTGGCGGAAAAGGGCGTCACCGAGCTGGTCGAGTGCGGGCCGGGCAAGGTCCTGGCAGGCCTCAACAGGCGC 
TGTGCGAAGGGCATCAATACCCATGGCCTGGATGGTGTCGAGGCGTTCGCCGCCACGCGCGCCGCCCTGGCC 
TGA