ViewGene
Locus Name:
GeneID:
Location:

GeneID:247
TrivialName: PA14_30230
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_30230
PAO1 Ortholog Locus:PA2620
Sequence Length:2277
Protein Length:758
Start: 2617332
Stop: 2619608
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06008.1|AE004690_8 (AE004690) ATP-binding protease component ClpA [Pseudomonas aeruginosa PAO1]
Identities = 758/758 (100%), Positives = 758/758 (100%)
Sequence:
ATGTTGAATCGAGAGCTCGAAGTCACCCTCAATCTCGCCTTCAAGGAGGCGAGGGCCAAACGCCATGAATTC
ATGACGGTTGAGCACCTGCTGCTGGCCTTGCTGGACAATGAGGCGGCGGCAACGGTATTGCGTGCGTGCGGT
GCCAACCTGGACAAGCTGCGGCGCGACCTGCAGGAATTCATCGATTCCACCACGCCGCTGATCCCGCAGCAC
GACGACGAGCGCGAAACCCAGCCGACGCTGGGCTTCCAGCGCGTCCTGCAGCGTGCGGTGTTCCACGTGCAG
AGTTCCGGCAAGCGCGAAGTGACCGGGGCCAATGTCCTGGTGGCGATCTTCAGCGAACAGGAAAGCCAGGCG
GTGTTCCTGCTCAAGCAGCAGAGCATCGCGCGTATCGATGTGGTGAACTACATCGCCCACGGCATCTCCAAG
GTGCCCGGGCATGCCGAACATCCGCAGGATGGGGAGCAGGATATGCAGGATGAGGAAGGTGGCGAGTCGGCC
ACGTCCAACCATCCGCTGGACGCCTATGCCAGCAACCTCAACGAACTGGCTCGCCAGGGGCGGATCGACCCG
CTGGTGGGGCGTGAGCATGAAGTCGAGCGGGTGGCGCAGATCCTTGCCCGCCGGCGCAAGAACAACCCGCTG
CTGGTAGGCGAGGCGGGGGTCGGCAAGACGGCCATCGCCGAAGGCCTGGCCAAGCGCATCGTCGATGGCCAG
GTGCCGGACCTGCTGGCCGACAGCGTGGTCTACTCCCTCGACCTGGGTGCCTTGCTCGCGGGTACCAAGTAC
CGCGGCGACTTCGAGAAGCGCTTCAAGGCCTTGCTCAACGAGTTGCGCAAGCGCCCGCACGCGGTGCTGTTC
ATCGACGAGATCCATACCATCATCGGCGCCGGTGCAGCATCCGGCGGGGTAATGGACGCCTCCAACCTGCTC
AAGCCGGTTCTGTCCTCGGGCGAGATCCGCTGCATCGGCTCGACCACCTTCCAGGAGTTCCGCGGCATCTTC
GAGAAGGACCGGGCCTTGGCGCGGCGCTTCCAGAAGGTCGACGTGACCGAGCCGTCGGTGGAAGACACCTAT
GGCATCCTCAAGGGCCTCAAGGGGCGCTTCGAGCAGCATCACCACATCGAGTACAGCGACGAGGCGCTGCGC
GCCGCGGCCGAGCTGGCGGCGCGCTACATCAACGACCGGCACATGCCGGACAAGGCCATCGACGTCATCGAC
GAGGCGGGCGCCTACCAGCGCCTGCAGCCGGAAGAGAAGCGCGTGAAGCGCATCGAGGTGGCGCAGGTCGAG
GATATCGTGGCGAAGATCGCGCGGATCCCGCCGAAACACGTCACCACCTCGGACAAGGAGTTGCTGCGCAAC
CTCGAACGCGACCTCAAGCTGACCGTGTTCGGCCAGGACGACGCCATCGAGTCGCTGTCCACCGCGATCAAG
CTGTCCCGGGCCGGGCTCAAGGCGCCGGACAAGCCGGTCGGCTCGTTCCTCTTCGCCGGTCCCACCGGCGTG
GGCAAGACCGAAGTGGCGCGGCAGTTGGCGAAGGCCTTGGGCGTGGAACTGGTGCGCTTCGACATGTCCGAG
TACATGGAGCGGCATACCGTGTCGCGGCTGATCGGTGCGCCTCCGGGCTACGTCGGCTTCGACCAGGGCGGC
CTGCTCACCGAGGCGATCACCAAGACCCCGCACTGCGTGTTGCTGCTCGACGAGATCGAGAAGGCTCACCCG
GAGGTCTTCAACCTGCTGCTGCAGGTGATGGACCACGGCACCCTGACCGACAACAACGGGCGCAAGGCGGAC
TTCCGCAACATCATCCTGATCATGACCACCAACGCCGGCGCGGAAGTGGCGGCGCGCGCGTCGATCGGCTTC
AACCAGCAGGATCACACCACCGATGCGATGGAAGTGATCAAGAAGAGCTTCACCCCGGAGTTCCGCAACCGC
CTGGATACCATCATCCAGTTCGGCCGCCTGAGCACCGAGACGATCAAGAGCGTGGTCGACAAGTTCCTCACC
GAGCTGCAGGCGCAGCTGGAGGACAAGCGCGTCCAGCTCGAGGTCAGCGATGCGGCGCGCGGCTGGCTGGCG
GAGAAGGGCTACGACGTGCAGATGGGTGCGCGACCGATGGCGCGGCTTATCCAGGACAAGATCAAGCGGCCG
TTGGCCGAGGAGATCCTGTTCGGCGAGCTGGCCGAGCATGGCGGCCTGGTGCATGTCGACCTGAAGGGCGAC
GAGCTGGCCTTCGAGTTCGAGATCACGGCGGCGGAGCCCGCCTGA
Translation:
MLNRELEVTLNLAFKEARAKRHEFMTVEHLLLALLDNEAAATVLRACGANLDKLRRDLQEFIDSTTPLIPQH
DDERETQPTLGFQRVLQRAVFHVQSSGKREVTGANVLVAIFSEQESQAVFLLKQQSIARIDVVNYIAHGISK
VPGHAEHPQDGEQDMQDEEGGESATSNHPLDAYASNLNELARQGRIDPLVGREHEVERVAQILARRRKNNPL
LVGEAGVGKTAIAEGLAKRIVDGQVPDLLADSVVYSLDLGALLAGTKYRGDFEKRFKALLNELRKRPHAVLF
IDEIHTIIGAGAASGGVMDASNLLKPVLSSGEIRCIGSTTFQEFRGIFEKDRALARRFQKVDVTEPSVEDTY
GILKGLKGRFEQHHHIEYSDEALRAAAELAARYINDRHMPDKAIDVIDEAGAYQRLQPEEKRVKRIEVAQVE
DIVAKIARIPPKHVTTSDKELLRNLERDLKLTVFGQDDAIESLSTAIKLSRAGLKAPDKPVGSFLFAGPTGV
GKTEVARQLAKALGVELVRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAITKTPHCVLLLDEIEKAHP
EVFNLLLQVMDHGTLTDNNGRKADFRNIILIMTTNAGAEVAARASIGFNQQDHTTDAMEVIKKSFTPEFRNR
LDTIIQFGRLSTETIKSVVDKFLTELQAQLEDKRVQLEVSDAARGWLAEKGYDVQMGARPMARLIQDKIKRP
LAEEILFGELAEHGGLVHVDLKGDELAFEFEITAAEPA*
AnnotationID:257GeneID:247
AnnotatorUID: diggins
Modification Date/Time: 2005-03-07 13:01:38
Gene Name:clpA
Confidence Code:2
GeneProduct:ATP-dependent clp protease, ATP-binding subunit ClpA
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0542
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO56831.1| (AE016868) ATP-dependent clp protease, ATP-binding subunit ClpA
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 757

 Score = 1346 bits (3483), Expect = 0.0
 Identities = 685/754 (90%), Positives = 721/754 (95%)

>gb|AAN69602.1|AE016593_10 (AE016788) ATP-dependent Clp protease, ATP-binding subunit ClpA
           [Pseudomonas putida KT2440]
          Length = 756

 Score = 1345 bits (3480), Expect = 0.0
 Identities = 689/754 (91%), Positives = 720/754 (95%), Gaps = 1/754 (0%)

>gb|AAN55655.1|AE015704_6 (AE015704) ATP-dependent Clp protease, ATP-binding subunit ClpA
           [Shewanella oneidensis MR-1]
          Length = 755

 Score = 1021 bits (2641), Expect = 0.0






 
Structural Features:
COG0542, ClpA, ATPases with chaperone activity, ATP-binding subunit 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 786 residues,  99.7% aligned
Score =  872 bits (2254), Expect = 0.0
Identities = 390/809 (48%), Positives = 519/809 (64%), Gaps = 83/809 (10%)






   
Genomic Context:






   
Comment:


















    ReferenceID:497




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17639




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33186




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:247_cdd.html


    		




 
URL:

















    ReferenceID:34556




  
Author/Investigator(s):
        Gottesman S, Clark WP, Maurizi MR.





  
Title:
        The ATP-dependent Clp protease of Escherichia coli. Sequence of clpA and identification of a Clp-specific substrate.




  
PubMed:
        2186030


MedLine:
        




    
Source:
J Biol Chem. 1990 May 15;265(14):7886-93.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2186030

















    ReferenceID:34557




  
Author/Investigator(s):
        Katayama Y, Gottesman S, Pumphrey J, Rudikoff S, Clark WP, Maurizi MR.





  
Title:
        The two-component, ATP-dependent Clp protease of Escherichia coli. Purification, cloning, and mutational analysis of the ATP-binding component.




  
PubMed:
        3049606


MedLine:
        




    
Source:
J Biol Chem. 1988 Oct 15;263(29):15226-36.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3049606

















    ReferenceID:34558




  
Author/Investigator(s):
        Shrader TE, Tobias JW, Varshavsky A.





  
Title:
        The N-end rule in Escherichia coli: cloning and analysis of the leucyl, phenylalanyl-tRNA-protein transferase gene aat.




  
PubMed:
        8331068


MedLine:
        




    
Source:
J Bacteriol. 1993 Jul;175(14):4364-74.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8331068









           
Homologs By Global Alignment 

           

       



     
Gene ID:247
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
419
                           PA2620_tr
                           translation of PA2620
                           758
                           99.99
                           99.99
                           0
                           3817.0
     

     
     
23052
                           gb|AAG06008.1|AE004690_8
                           (AE004690) ATP-binding protease component ClpA [Pseudomonas aeruginosa PAO1]
                           758
                           99.99
                           99.99
                           0
                           3817.0
     

     
     
23054
                           gb|AAN69602.1|AE016593_10
                           (AE016788) ATP-dependent Clp protease, ATP-binding subunit ClpA [Pseudomonas putida KT2440]
                           758
                           91.16
                           95.25
                           2
                           3480.0
     

     
     
23053
                           gb|AAO56831.1|
                           (AE016868) ATP-dependent clp protease, ATP-binding subunit ClpA [Pseudomonas syringae pv. tomato str. DC3000]
                           758
                           90.63
                           95.38
                           1
                           3481.0
     

     
     
23055
                           gb|AAN55655.1|AE015704_6
                           (AE015704) ATP-dependent Clp protease, ATP-binding subunit ClpA [Shewanella oneidensis MR-1]
                           761
                           68.33
                           80.68
                           9
                           2643.0
     

     






      

          
GC ORFID: 43249How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2620Glimmer Score: 

          
Start: 2336594Stop: 2334318

          
Length: 2277


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2620 translation of PA2620Homolog Bit Score 1474.0

          
Other Homologs:  psyr_15may02_Scaffold17_revised_gene2808 (ORF: BLASTX 1347.0), t_PA2620 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTGAATCGAGAGCTCGAAGTCACCCTCAATCTCGCCTTCAAGGAGGCGAGGGCCAAACGCCATGAATTC 
ATGACGGTTGAGCACCTGCTGCTGGCCTTGCTGGACAATGAGGCGGCGGCAACGGTATTGCGTGCGTGCGGT 
GCCAACCTGGACAAGCTGCGGCGCGACCTGCAGGAATTCATCGATTCCACCACGCCGCTGATCCCGCAGCAC 
GACGACGAGCGCGAAACCCAGCCGACGCTGGGCTTCCAGCGCGTCCTGCAGCGTGCGGTGTTCCACGTGCAG 
AGTTCCGGCAAGCGCGAAGTGACCGGGGCCAATGTCCTGGTGGCGATCTTCAGCGAACAGGAAAGCCAGGCG 
GTGTTCCTGCTCAAGCAGCAGAGCATCGCGCGTATCGATGTGGTGAACTACATCGCCCACGGCATCTCCAAG 
GTGCCCGGGCATGCCGAACATCCGCAGGATGGGGAGCAGGATATGCAGGATGAGGAAGGTGGCGAGTCGGCC 
ACGTCCAACCATCCGCTGGACGCCTATGCCAGCAACCTCAACGAACTGGCTCGCCAGGGGCGGATCGACCCG 
CTGGTGGGGCGTGAGCATGAAGTCGAGCGGGTGGCGCAGATCCTTGCCCGCCGGCGCAAGAACAACCCGCTG 
CTGGTAGGCGAGGCGGGGGTCGGCAAGACGGCCATCGCCGAAGGCCTGGCCAAGCGCATCGTCGATGGCCAG 
GTGCCGGACCTGCTGGCCGACAGCGTGGTCTACTCCCTCGACCTGGGTGCCTTGCTCGCGGGTACCAAGTAC 
CGCGGCGACTTCGAGAAGCGCTTCAAGGCCTTGCTCAACGAGTTGCGCAAGCGCCCGCACGCGGTGCTGTTC 
ATCGACGAGATCCATACCATCATCGGCGCCGGTGCAGCATCCGGCGGGGTAATGGACGCCTCCAACCTGCTC 
AAGCCGGTTCTGTCCTCGGGCGAGATCCGCTGCATCGGCTCGACCACCTTCCAGGAGTTCCGCGGCATCTTC 
GAGAAGGACCGGGCCTTGGCGCGGCGCTTCCAGAAGGTCGACGTGACCGAGCCGTCGGTGGAAGACACCTAT 
GGCATCCTCAAGGGCCTCAAGGGGCGCTTCGAGCAGCATCACCACATCGAGTACAGCGACGAGGCGCTGCGC 
GCCGCGGCCGAGCTGGCGGCGCGCTACATCAACGACCGGCACATGCCGGACAAGGCCATCGACGTCATCGAC 
GAGGCGGGCGCCTACCAGCGCCTGCAGCCGGAAGAGAAGCGCGTGAAGCGCATCGAGGTGGCGCAGGTCGAG 
GATATCGTGGCGAAGATCGCGCGGATCCCGCCGAAACACGTCACCACCTCGGACAAGGAGTTGCTGCGCAAC 
CTCGAACGCGACCTCAAGCTGACCGTGTTCGGCCAGGACGACGCCATCGAGTCGCTGTCCACCGCGATCAAG 
CTGTCCCGGGCCGGGCTCAAGGCGCCGGACAAGCCGGTCGGCTCGTTCCTCTTCGCCGGTCCCACCGGCGTG 
GGCAAGACCGAAGTGGCGCGGCAGTTGGCGAAGGCCTTGGGCGTGGAACTGGTGCGCTTCGACATGTCCGAG 
TACATGGAGCGGCATACCGTGTCGCGGCTGATCGGTGCGCCTCCGGGCTACGTCGGCTTCGACCAGGGCGGC 
CTGCTCACCGAGGCGATCACCAAGACCCCGCACTGCGTGTTGCTGCTCGACGAGATCGAGAAGGCTCACCCG 
GAGGTCTTCAACCTGCTGCTGCAGGTGATGGACCACGGCACCCTGACCGACAACAACGGGCGCAAGGCGGAC 
TTCCGCAACATCATCCTGATCATGACCACCAACGCCGGCGCGGAAGTGGCGGCGCGCGCGTCGATCGGCTTC 
AACCAGCAGGATCACACCACCGATGCGATGGAAGTGATCAAGAAGAGCTTCACCCCGGAGTTCCGCAACCGC 
CTGGATACCATCATCCAGTTCGGCCGCCTGAGCACCGAGACGATCAAGAGCGTGGTCGACAAGTTCCTCACC 
GAGCTGCAGGCGCAGCTGGAGGACAAGCGCGTCCAGCTCGAGGTCAGCGATGCGGCGCGCGGCTGGCTGGCG 
GAGAAGGGCTACGACGTGCAGATGGGTGCGCGACCGATGGCGCGGCTTATCCAGGACAAGATCAAGCGGCCG 
TTGGCCGAGGAGATCCTGTTCGGCGAGCTGGCCGAGCATGGCGGCCTGGTGCATGTCGACCTGAAGGGCGAC 
GAGCTGGCCTTCGAGTTCGAGATCACGGCGGCGGAGCCCGCCTGA