ViewGene
Locus Name:
GeneID:
Location:

GeneID:245
TrivialName: PA14_04410
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_04410
PAO1 Ortholog Locus:PA0337
Sequence Length:2280
Protein Length:759
Start: 392770
Stop: 395049
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03726.1|AE004471_9 (AE004471) phosphoenolpyruvate-protein phosphotransferase PtsP [Pseudomonas aeruginosa PAO1]
Identities = 759/759 (100%), Positives = 759/759 (100%)
Sequence:
ATGCTCAACACGCTGCGCAAGATCGTCCAGGAAGTGAACTCCGCCAAGGATCTGAAGGCGGCGCTGGGCATC
ATCGTGCAGCGGGTCAAGGAAGCCATGGGTACCCAGGTCTGCTCGGTGTACCTGCTCGACACCGAGACCCAG
CGTTTCGTCCTGATGGCCACCGAAGGCCTCAACAAGCGTTCCATCGGCAAGGTCAGCATGGCCCCCAGCGAA
GGCCTGGTCGGCCTGGTCGGCACCCGCGAGGAGCCGCTCAACCTGGAGAACGCCGCCGCCCACCCGCGCTAC
CGCTATTTCGCCGAGACCGGCGAGGAGCGCTACGCGTCGTTCCTCGGCGCGCCGATCATCCACCATAGGCGG
GTGATGGGGGTGCTGGTGGTGCAGCAGAAGGAGCGCCGCCAGTTCGACGAAGGCGAGGAGGCCTTCCTCGTC
ACCATGAGCGCCCAGCTCGCCGGGGTCATCGCGCATGCCGAGGCGACCGGTTCGATCCGCGGCCTGGGCAAG
CTCGGCAAGGGCATCCAGGAAGCCAAGTTCGTCGGCGTGCCCGGCGCCCCCGGGGTCGGGGTGGGCAAGGCG
GTGGTGGTGTTGCCTCCGGCCGACCTGGAAGTGGTGCCGGACAAGCAGGTCGACGACATCGACGCCGAGATC
GCCCTGTTCAAGCAGGCCCTGGAGGGCGTTCGCGCCGACATGCGCGCGCTGTCGAGCAAGCTCGCCAGCCAG
TTGCGCAAGGAAGAACGCGCGCTGTTCGACGTCTACCTGATGATGCTCGACGATGCCTCCATCGGCAACGAG
GTCAAGCGCATCATCCGTACCGGCCAGTGGGCCCAGGGCGCCCTGCGCCAGGTGGTGATGGAGCACGTGCAG
CGCTTCGAGCTGATGGACGACGCCTATCTCCGCGAGCGCGCCTCCGACGTCAAGGACATCGGTCGCCGCCTG
CTCGCCTACCTGCAGGAAGAACGCAAGCAGAACCTGACCTACCCGGAGCAGACCATCATCGTCAGCGAGGAG
CTGTCGCCGGCGATGCTCGGCGAGGTGCCGGAAGGGCGCCTGGTCGGCCTGGTCTCGGTGCTCGGCTCGGGC
AACTCGCACGTGGCGATCCTCGCCCGTGCCATGGGCATCCCCACGGTGATGGGGGCGGTCGACCTGCCGTAC
TCCAAGGTCGACGGTATCGACCTGATCGTCGATGGCTACCACGGCGAGGTCTACACCAACCCCTCCGCCGAG
CTGGTGCGCCAGTACAGCGACGTGGTCGCCGAGGAGCGCGAGCTGAGCAAGGGCCTGGCGGCCCTGCGCGAG
CTGCCCTGCGAGACCCTCGACGGCCACCGCATGCCGCTCTGGGTCAACACCGGCCTGCTCGCCGATGTCGCC
CGCGCCCAGGAGCGTGGCGCCGAGGGCGTGGGCCTGTACCGCACCGAAGTGCCGTTCATGATCAACGACCGC
TTCCCCAGCGAGAAGGAACAGCTGGCGATCTACCGCGAGCAGCTCAGTGCCTTCCACCCGCTGCCGGTGACC
ATGCGCACCCTGGATATCGGCGGCGACAAGGCGCTGTCCTACTTCCCGATCAAGGAAGACAACCCGTTCCTC
GGCTGGCGCGGCATCCGCGTCACCCTCGACCACCCGGAGATCTTCCTGGTCCAGACCCGCGCCATGCTCAAG
GCCAGCGAAGGACTGGACAACCTGCGCATCCTGCTGCCGATGATCTCCGGCACCCACGAGCTGGAAGAGGCC
CTGCACCTGATCCACCGCGCCTGGGGCGAGGTGCGCGACGAGGGCGTGGACATCGCCATGCCGCCTATCGGC
ATGATGGTCGAGATTCCCGCCGCCGTGTACCAGACCCGCGAGCTGGCCCGTCAGGTCGACTTCCTTTCGGTC
GGTTCGAACGACCTGACCCAGTACCTGCTGGCGGTCGACCGCAACAATCCGCGGGTCGCCGACCTCTACGAC
TACCTGCATCCGGCCGTGCTGCATGCGTTGAAGAAGGTGGTCGACGATGCCCACCTGGAAGGCAAGCCGGTG
AGCATCTGCGGCGAGATGGCCGGCGATCCCGCGGCTGCCGTGCTGCTGATGGCGATGGGCTTCGACAGCCTG
TCGATGAACGCCACCAACCTGCCCAAGGTGAAGTGGCTGCTGCGCCAGATCACCCTGGACAAGGCCCGGGAC
CTGCTCGGCCAGTTGCTCACCTTCGACAACCCGCAGGTCATCCACAGCTCGCTGCACCTGGCGTTGCGCAAC
CTCGGCCTGGGTCGCGTGATCAACCCGGCGGCTACCGTCCAGCCCTGA
Translation:
MLNTLRKIVQEVNSAKDLKAALGIIVQRVKEAMGTQVCSVYLLDTETQRFVLMATEGLNKRSIGKVSMAPSE
GLVGLVGTREEPLNLENAAAHPRYRYFAETGEERYASFLGAPIIHHRRVMGVLVVQQKERRQFDEGEEAFLV
TMSAQLAGVIAHAEATGSIRGLGKLGKGIQEAKFVGVPGAPGVGVGKAVVVLPPADLEVVPDKQVDDIDAEI
ALFKQALEGVRADMRALSSKLASQLRKEERALFDVYLMMLDDASIGNEVKRIIRTGQWAQGALRQVVMEHVQ
RFELMDDAYLRERASDVKDIGRRLLAYLQEERKQNLTYPEQTIIVSEELSPAMLGEVPEGRLVGLVSVLGSG
NSHVAILARAMGIPTVMGAVDLPYSKVDGIDLIVDGYHGEVYTNPSAELVRQYSDVVAEERELSKGLAALRE
LPCETLDGHRMPLWVNTGLLADVARAQERGAEGVGLYRTEVPFMINDRFPSEKEQLAIYREQLSAFHPLPVT
MRTLDIGGDKALSYFPIKEDNPFLGWRGIRVTLDHPEIFLVQTRAMLKASEGLDNLRILLPMISGTHELEEA
LHLIHRAWGEVRDEGVDIAMPPIGMMVEIPAAVYQTRELARQVDFLSVGSNDLTQYLLAVDRNNPRVADLYD
YLHPAVLHALKKVVDDAHLEGKPVSICGEMAGDPAAAVLLMAMGFDSLSMNATNLPKVKWLLRQITLDKARD
LLGQLLTFDNPQVIHSSLHLALRNLGLGRVINPAATVQP*
AnnotationID:255GeneID:245
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 15:03:09
Gene Name:ptsP
Confidence Code:2
GeneProduct:phosphoenolpyruvate-protein phosphotransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3605,COG1080
Secondary Functional Category(ies):
EC Number:2.7.3.9
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58710.1| (AE016875) phosphoenolpyruvate-protein phosphotransferase PtsP
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 759

 Score = 1296 bits (3353), Expect = 0.0
 Identities = 652/755 (86%), Positives = 703/755 (93%)

>gb|AAN70710.1|AE016714_8 (AE016793) phosphoenolpyruvate-protein phosphotransferase PtsP
           [Pseudomonas putida KT2440]
          Length = 759

 Score = 1295 bits (3352), Expect = 0.0
 Identities = 654/755 (86%), Positives = 704/755 (93%)

>gb|AAO91047.1| (AE016964) phosphoenolpyruvate-protein phosphotransferase [Coxiella
           burnetii RSA 493]
          Length = 759

 Score =  808 bits (2086), Expect = 0.0
 Identities = 407/753 (54%), Positives = 543/753 (72%), Gaps = 2/753 (0%)








 
Structural Features:
COG3605, PtsP, Signal transduction protein containing GAF and PtsI domains 
[Signal transduction mechanisms]
CD-Length = 756 residues, 100.0% aligned
Score = 1044 bits (2701), Expect = 0.0
Identities = 383/756 (50%), Positives = 497/756 (65%), Gaps = 1/756 (0%)

COG1080, PtsA, Phosphoenolpyruvate-protein kinase 
(PTS system EI component in bacteria) 
[Carbohydrate transport and metabolism]
CD-Length = 574 residues,  97.9% aligned
Score =  529 bits (1365), Expect = 3e-151
Identities = 238/564 (42%), Positives = 352/564 (62%), Gaps = 7/564 (1%)







   
Genomic Context:






   
Comment:


















    ReferenceID:493




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17635




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33680




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:245_cdd.html


    		




 
URL:

















    ReferenceID:35419




  
Author/Investigator(s):
        Reizer J, Reizer A, Merrick MJ, Plunkett G 3rd, Rose DJ, Saier MH Jr.





  
Title:
        Novel phosphotransferase-encoding genes revealed by analysis of the Escherichia coli genome: a chimeric gene encoding an Enzyme I homologue that possesses a putative sensory transduction domain.




  
PubMed:
        8973315


MedLine:
        




    
Source:
Gene. 1996 Nov 28;181(1-2):103-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8973315

















    ReferenceID:35420




  
Author/Investigator(s):
        Rabus R, Reizer J, Paulsen I, Saier MH Jr.





  
Title:
        Enzyme I(Ntr) from Escherichia coli. A novel enzyme of the phosphoenolpyruvate-dependent phosphotransferase system exhibiting strict specificity for its phosphoryl acceptor, NPr.




  
PubMed:
        10473571


MedLine:
        




    
Source:
J Biol Chem. 1999 Sep 10;274(37):26185-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10473571

















    ReferenceID:35421




  
Author/Investigator(s):
        Higa F, Edelstein PH.





  
Title:
        Potential virulence role of the Legionella pneumophila ptsP ortholog.




  
PubMed:
        11447151


MedLine:
        




    
Source:
Infect Immun. 2001 Aug;69(8):4782-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11447151









           
Homologs By Global Alignment 

           

       



     
Gene ID:245
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
415
                           PA0337_tr
                           translation of PA0337
                           759
                           99.99
                           99.99
                           0
                           3832.0
     

     
     
23019
                           gb|AAG03726.1|AE004471_9
                           (AE004471) phosphoenolpyruvate-protein phosphotransferase PtsP [Pseudomonas aeruginosa PAO1]
                           759
                           99.99
                           99.99
                           0
                           3832.0
     

     
     
23020
                           gb|AAD22459.1|AF116285_2
                           (AF116285) phosphoenolpyruvate-protein phosphotransferase PtsP [Pseudomonas aeruginosa]
                           759
                           99.99
                           99.99
                           0
                           3832.0
     

     
     
23021
                           emb|CAA74995.1|
                           (Y14681) enzyme I [Azotobacter vinelandii]
                           759
                           86.69
                           93.54
                           0
                           3373.0
     

     
     
23023
                           gb|AAN70710.1|AE016714_8
                           (AE016793) phosphoenolpyruvate-protein phosphotransferase PtsP [Pseudomonas putida KT2440]
                           761
                           86.07
                           92.77
                           4
                           3347.0
     

     






      

          
GC ORFID: 43247How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0337Glimmer Score: 

          
Start: 6194112Stop: 6196391

          
Length: 2280


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0337 translation of PA0337Homolog Bit Score 1480.0

          
Other Homologs:  psyr_15may02_Scaffold6_revised_gene6532 (ORF: BLASTX 1296.0), t_PA0337 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCTCAACACGCTGCGCAAGATCGTCCAGGAAGTGAACTCCGCCAAGGATCTGAAGGCGGCGCTGGGCATC 
ATCGTGCAGCGGGTCAAGGAAGCCATGGGTACCCAGGTCTGCTCGGTGTACCTGCTCGACACCGAGACCCAG 
CGTTTCGTCCTGATGGCCACCGAAGGCCTCAACAAGCGTTCCATCGGCAAGGTCAGCATGGCCCCCAGCGAA 
GGCCTGGTCGGCCTGGTCGGCACCCGCGAGGAGCCGCTCAACCTGGAGAACGCCGCCGCCCACCCGCGCTAC 
CGCTATTTCGCCGAGACCGGCGAGGAGCGCTACGCGTCGTTCCTCGGCGCGCCGATCATCCACCATAGGCGG 
GTGATGGGGGTGCTGGTGGTGCAGCAGAAGGAGCGCCGCCAGTTCGACGAAGGCGAGGAGGCCTTCCTCGTC 
ACCATGAGCGCCCAGCTCGCCGGGGTCATCGCGCATGCCGAGGCGACCGGTTCGATCCGCGGCCTGGGCAAG 
CTCGGCAAGGGCATCCAGGAAGCCAAGTTCGTCGGCGTGCCCGGCGCCCCCGGGGTCGGGGTGGGCAAGGCG 
GTGGTGGTGTTGCCTCCGGCCGACCTGGAAGTGGTGCCGGACAAGCAGGTCGACGACATCGACGCCGAGATC 
GCCCTGTTCAAGCAGGCCCTGGAGGGCGTTCGCGCCGACATGCGCGCGCTGTCGAGCAAGCTCGCCAGCCAG 
TTGCGCAAGGAAGAACGCGCGCTGTTCGACGTCTACCTGATGATGCTCGACGATGCCTCCATCGGCAACGAG 
GTCAAGCGCATCATCCGTACCGGCCAGTGGGCCCAGGGCGCCCTGCGCCAGGTGGTGATGGAGCACGTGCAG 
CGCTTCGAGCTGATGGACGACGCCTATCTCCGCGAGCGCGCCTCCGACGTCAAGGACATCGGTCGCCGCCTG 
CTCGCCTACCTGCAGGAAGAACGCAAGCAGAACCTGACCTACCCGGAGCAGACCATCATCGTCAGCGAGGAG 
CTGTCGCCGGCGATGCTCGGCGAGGTGCCGGAAGGGCGCCTGGTCGGCCTGGTCTCGGTGCTCGGCTCGGGC 
AACTCGCACGTGGCGATCCTCGCCCGTGCCATGGGCATCCCCACGGTGATGGGGGCGGTCGACCTGCCGTAC 
TCCAAGGTCGACGGTATCGACCTGATCGTCGATGGCTACCACGGCGAGGTCTACACCAACCCCTCCGCCGAG 
CTGGTGCGCCAGTACAGCGACGTGGTCGCCGAGGAGCGCGAGCTGAGCAAGGGCCTGGCGGCCCTGCGCGAG 
CTGCCCTGCGAGACCCTCGACGGCCACCGCATGCCGCTCTGGGTCAACACCGGCCTGCTCGCCGATGTCGCC 
CGCGCCCAGGAGCGTGGCGCCGAGGGCGTGGGCCTGTACCGCACCGAAGTGCCGTTCATGATCAACGACCGC 
TTCCCCAGCGAGAAGGAACAGCTGGCGATCTACCGCGAGCAGCTCAGTGCCTTCCACCCGCTGCCGGTGACC 
ATGCGCACCCTGGATATCGGCGGCGACAAGGCGCTGTCCTACTTCCCGATCAAGGAAGACAACCCGTTCCTC 
GGCTGGCGCGGCATCCGCGTCACCCTCGACCACCCGGAGATCTTCCTGGTCCAGACCCGCGCCATGCTCAAG 
GCCAGCGAAGGACTGGACAACCTGCGCATCCTGCTGCCGATGATCTCCGGCACCCACGAGCTGGAAGAGGCC 
CTGCACCTGATCCACCGCGCCTGGGGCGAGGTGCGCGACGAGGGCGTGGACATCGCCATGCCGCCTATCGGC 
ATGATGGTCGAGATTCCCGCCGCCGTGTACCAGACCCGCGAGCTGGCCCGTCAGGTCGACTTCCTTTCGGTC 
GGTTCGAACGACCTGACCCAGTACCTGCTGGCGGTCGACCGCAACAATCCGCGGGTCGCCGACCTCTACGAC 
TACCTGCATCCGGCCGTGCTGCATGCGTTGAAGAAGGTGGTCGACGATGCCCACCTGGAAGGCAAGCCGGTG 
AGCATCTGCGGCGAGATGGCCGGCGATCCCGCGGCTGCCGTGCTGCTGATGGCGATGGGCTTCGACAGCCTG 
TCGATGAACGCCACCAACCTGCCCAAGGTGAAGTGGCTGCTGCGCCAGATCACCCTGGACAAGGCCCGGGAC 
CTGCTCGGCCAGTTGCTCACCTTCGACAACCCGCAGGTCATCCACAGCTCGCTGCACCTGGCGTTGCGCAAC 
CTCGGCCTGGGTCGCGTGATCAACCCGGCGGCTACCGTCCAGCCCTGA