ViewGene
Locus Name:
GeneID:
Location:

GeneID:2448
TrivialName: PA14_48000
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_48000
PAO1 Ortholog Locus:PA1254
Sequence Length:918
Protein Length:305
Start: 4271307
Stop: 4272224
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04643.1|AE004555_3 (AE004555) probable dihydrodipicolinate synthetase [Pseudomonas aeruginosa PAO1]
Identities = 303/305 (99%), Positives = 304/305 (99%)
Sequence:
ATGAGCAAGCACGTCAACTGGAGCGGCGTATTCCCCGCGGTCACCACCCAATTCAACGCCGATTTCTCGGTG
AACCTGGAGGAAACCCACACGGTCATTTCCAACCTGGTGCGCGACGGCGTGTCCGGGCTGGTGGTATGCGGC
AGCGTCGGCGAGAACACCTCGCTGACCATCGAGGAGAAGATGGCCGTCACCGAGGTCGCCAAGGATGCCTCC
GGCGGCCGCGTGCCGGTGATCTGCGGGATCGCCGAGTTCACCAGCGCCGGCGCGGCGAAGGTGGCCAAGGCG
GTGGAGCGGGTCGGCGTCGACGGCATCATGGTGATGCCGGCGCTGGTCTATTCCTCCAAGCCGCACGAGACC
GCCGAGCATTTCCGCAGCGTGGCTAGCGGCACCGACCTGCCGGTGATGGTCTACAACAACCCGCCGATCTAC
CGGAACGATGTCACCCCGGACATCCTGGTTTCCCTGGCCGACTGCGAGAACATCGTCTGCTTCAAGGACAGC
TCCGGCGACACCCGCCGCTTCATCGACGTACGCAACCAGGTGGGCGAGCGCTTCATCCTCTTCGCCGGCCTC
GACGACGTGGTCCTGGAAAGCGTGGCGGTGGGCGCTCAGGGCTGGATCTCGGGAATGTCCAACGTATTCCCG
AAGGAAGGCGAGACCATCTTCCGCCTGGCCAGGGCCGGGCGCTTCGCCGAGGCGATGCCGATCTACGAGTGG
CTGATGCCGATCCTCCATCTCGACGCCCGTCCCGACCTGGTGCAGTGCATCAAGCTCTGCGAGGAACTCGCC
GGTCGCGGCAGCGCCCTCACCCGTCCACCGCGCCTGGCCCTGCGCGGCGCCGATCGCGAGCACGTCGAGCGG
ATCATGGCCAAGGCCCTGGCCAGCCGGCCGGTGCTGCCGGACGTCGGCTTGTAG
Translation:
MSKHVNWSGVFPAVTTQFNADFSVNLEETHTVISNLVRDGVSGLVVCGSVGENTSLTIEEKMAVTEVAKDAS
GGRVPVICGIAEFTSAGAAKVAKAVERVGVDGIMVMPALVYSSKPHETAEHFRSVASGTDLPVMVYNNPPIY
RNDVTPDILVSLADCENIVCFKDSSGDTRRFIDVRNQVGERFILFAGLDDVVLESVAVGAQGWISGMSNVFP
KEGETIFRLARAGRFAEAMPIYEWLMPILHLDARPDLVQCIKLCEELAGRGSALTRPPRLALRGADREHVER
IMAKALASRPVLPDVGL*
AnnotationID:2456GeneID:2448
AnnotatorUID: diggins
Modification Date/Time: 2005-02-22 11:11:53
Gene Name:
Confidence Code:3
GeneProduct:putative dihydrodipicolinate synthetase
Cell Localization:()
Synonyms:PA1254
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0329,cd00408,pfam00701,cd00950
Secondary Functional Category(ies):central intermediary metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAC59598.1| (AP005077) probable dihydrodipicolinate synthetase [Vibrio
           parahaemolyticus]
          Length = 304

 Score =  293 bits (751), Expect = 7e-79
 Identities = 139/294 (47%), Positives = 205/294 (69%), Gaps = 1/294 (0%)

>gb|AAM41689.1| (AE012352) dihydrodipicolinate synthetase [Xanthomonas campestris
           pv. campestris str. ATCC 33913]
          Length = 302

 Score =  238 bits (606), Expect = 4e-62
 Identities = 121/301 (40%), Positives = 183/301 (60%), Gaps = 1/301 (0%)






 
Structural Features:
COG0329, DapA, Dihydrodipicolinate synthase/
N-acetylneuraminate lyase 
[Amino acid transport and metabolism / 
Cell envelope biogenesis, outer membrane]
CD-Length = 299 residues,  99.3% aligned
Score =  265 bits (678), Expect = 6e-72
Identities = 107/297 (36%), Positives = 162/297 (54%), Gaps = 2/297 (0%)

cd00408, DHDPS-like, Dihydrodipicolinate synthase family.
CD-Length = 281 residues, 100.0% aligned
Score =  262 bits (670), Expect = 5e-71

pfam00701, DHDPS, Dihydrodipicolinate synthetase family. 
CD-Length = 289 residues,  99.7% aligned
Score =  196 bits (499), Expect = 4e-51
Identities = 87/288 (30%), Positives = 148/288 (51%)

cd00950, DHDPS, Dihydrodipicolinate synthase (DHDPS) is a key enzyme 
in lysine biosynthesis.
CD-Length = 284 residues, 100.0% aligned
Score =  194 bits (494), Expect = 1e-50







   
Genomic Context:






   
Comment:


















    ReferenceID:4899




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22045




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37526




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2448_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2448
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4106
                           PA1254_tr
                           translation of PA1254
                           305
                           99.34
                           99.67
                           0
                           1554.0
     

     
     
122715
                           gb|AAG04643.1|AE004555_3
                           (AE004555) probable dihydrodipicolinate synthetase [Pseudomonas aeruginosa PAO1]
                           305
                           99.34
                           99.67
                           0
                           1554.0
     

     
     
122716
                           dbj|BAC49688.1|
                           (AP005951) bll4423 [Bradyrhizobium japonicum USDA 110]
                           308
                           71.10
                           84.41
                           3
                           1189.0
     

     
     
122717
                           emb|CAE14538.1|
                           (BX571866) unnamed protein product [Photorhabdus luminescens subsp. laumondii TTO1]
                           306
                           64.37
                           79.73
                           1
                           1057.0
     

     
     
122718
                           dbj|BAC59598.1|
                           (AP005077) probable dihydrodipicolinate synthetase [Vibrio parahaemolyticus]
                           306
                           46.07
                           67.64
                           3
                           750.0
     

     






      

          
GC ORFID: 45450How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1254Glimmer Score: 

          
Start: 682602Stop: 681685

          
Length: 918


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA1254 translation of PA1254Homolog Bit Score 603.0

          
Other Homologs:  t_PA1254 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCAAGCACGTCAACTGGAGCGGCGTATTCCCCGCGGTCACCACCCAATTCAACGCCGATTTCTCGGTG 
AACCTGGAGGAAACCCACACGGTCATTTCCAACCTGGTGCGCGACGGCGTGTCCGGGCTGGTGGTATGCGGC 
AGCGTCGGCGAGAACACCTCGCTGACCATCGAGGAGAAGATGGCCGTCACCGAGGTCGCCAAGGATGCCTCC 
GGCGGCCGCGTGCCGGTGATCTGCGGGATCGCCGAGTTCACCAGCGCCGGCGCGGCGAAGGTGGCCAAGGCG 
GTGGAGCGGGTCGGCGTCGACGGCATCATGGTGATGCCGGCGCTGGTCTATTCCTCCAAGCCGCACGAGACC 
GCCGAGCATTTCCGCAGCGTGGCTAGCGGCACCGACCTGCCGGTGATGGTCTACAACAACCCGCCGATCTAC 
CGGAACGATGTCACCCCGGACATCCTGGTTTCCCTGGCCGACTGCGAGAACATCGTCTGCTTCAAGGACAGC 
TCCGGCGACACCCGCCGCTTCATCGACGTACGCAACCAGGTGGGCGAGCGCTTCATCCTCTTCGCCGGCCTC 
GACGACGTGGTCCTGGAAAGCGTGGCGGTGGGCGCTCAGGGCTGGATCTCGGGAATGTCCAACGTATTCCCG 
AAGGAAGGCGAGACCATCTTCCGCCTGGCCAGGGCCGGGCGCTTCGCCGAGGCGATGCCGATCTACGAGTGG 
CTGATGCCGATCCTCCATCTCGACGCCCGTCCCGACCTGGTGCAGTGCATCAAGCTCTGCGAGGAACTCGCC 
GGTCGCGGCAGCGCCCTCACCCGTCCACCGCGCCTGGCCCTGCGCGGCGCCGATCGCGAGCACGTCGAGCGG 
ATCATGGCCAAGGCCCTGGCCAGCCGGCCGGTGCTGCCGGACGTCGGCTTGTAG