ViewGene
Locus Name:
GeneID:
Location:

GeneID:2443
TrivialName: PA14_11260
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_11260
PAO1 Ortholog Locus:PA4068
Sequence Length:930
Protein Length:309
Start: 979801
Stop: 980730
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07455.1|AE004823_1 (AE004823) probable epimerase [Pseudomonas aeruginosa PAO1]
Identities = 308/309 (99%), Positives = 308/309 (99%)
Sequence:
ATGTTTGAACGCCCCATCCTGATCACTGGCGGCGCCGGTTTCATCGGCTCCCACCTGACCGACGCGCTGCTG
GCGCGCGGCTACCGCGTGCGTGTGCTGGACAACCTGTCCACCGGCAAGCGCGACAACCTGCCCCTGGACGAC
GAACGGGTAGAGCTGCTCGAAGGCGACGCCGCCGACGCGGCGACCCTGGCGGCCGCCCTGAAGGGCTGCGCG
GCGGTCGCCCACCTGGCCGCGGTCGCCTCGGTACAGGCCTCGGTGGACGACCCGGTCGCCACCCACCAGAGC
AATTTCATCGCCACCCTGAACCTCTGCGAAGCGATGCGCGAGCAGGGCGTGAAGCGCGTGGTGTTCGCTTCC
AGCGCAGCGGTCTACGGGCAGAACGGCGAAGGCACGGCGATCGACGAAGACACCCCGAAGTCGCCGCTGACG
CCCTACGCGTCGGACAAGCTGGCCAGCGAGTACTACCTCGATTTCTATCGCCGCCAGCATGGGCTGGAACCG
GCGATCTTCCGCTTCTTCAACGTCTACGGGCCGCGCCAGGACCCGTCCTCGCCCTATTCCGGGGTGATCAGC
ATATTCACCGAGCGCGCCCTGAAGGGGACGCCGATCACCGTGTTCGGCGACGGCGAGCAGACCCGCGACTTC
ATCTACGTCGCCGACCTGGTCGATTTCCTGGTCCAGGCGCTGGAGGCGACGGCGGTGGAGCCCGGGGCGGTG
AACGTCGGCCTGAATCGCAGCATCAGCCTGAAGCAGTTGCTGGCGGAGATCGGCCAGGTGCTCGGTGGCCTG
CCGCCGGTGACCCACGCCGACGCGCGAGCCGGTGACATCAGGCATTCGCGGGCGAACAACGAGCGCCTGCTG
CGGCGTTTCTGCTTCGACCAGCCGACGCCGATGGGCGTGGGCCTGGCGCGCCTGCTCGGCCGCTGA
Translation:
MFERPILITGGAGFIGSHLTDALLARGYRVRVLDNLSTGKRDNLPLDDERVELLEGDAADAATLAAALKGCA
AVAHLAAVASVQASVDDPVATHQSNFIATLNLCEAMREQGVKRVVFASSAAVYGQNGEGTAIDEDTPKSPLT
PYASDKLASEYYLDFYRRQHGLEPAIFRFFNVYGPRQDPSSPYSGVISIFTERALKGTPITVFGDGEQTRDF
IYVADLVDFLVQALEATAVEPGAVNVGLNRSISLKQLLAEIGQVLGGLPPVTHADARAGDIRHSRANNERLL
RRFCFDQPTPMGVGLARLLGR*
AnnotationID:2451GeneID:2443
AnnotatorUID: diggins
Modification Date/Time: 2005-03-30 06:06:29
Gene Name:
Confidence Code:2
GeneProduct:putative NAD-dependent epimerase
Cell Localization:()
Synonyms:PA4068
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam01370,COG0451,COG1087,COG1088
Secondary Functional Category(ies):cell envelope biogenesis, outer membrane
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66129.1|AE016241_6 (AE016775) NAD-dependent epimerase/dehydratase family protein
           [Pseudomonas putida KT2440]
          Length = 310

 Score =  446 bits (1147), Expect = e-125
 Identities = 221/309 (71%), Positives = 257/309 (83%)

>gb|AAO54220.1| (AE016858) NAD-dependent epimerase/dehydratase family protein
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 309

 Score =  434 bits (1116), Expect = e-121
 Identities = 215/309 (69%), Positives = 247/309 (79%)






 
Structural Features:
pfam01370, Epimerase, NAD dependent epimerase/dehydratase family.
CD-Length = 310 residues,  91.9% aligned
Score =  242 bits (619), Expect = 4e-65
Identities = 108/290 (37%), Positives = 146/290 (50%), Gaps = 11/290 (3%)

COG0451, WcaG, Nucleoside-diphosphate-sugar epimerases 
[Cell envelope biogenesis, outer membrane / 
Carbohydrate transport and metabolism]
CD-Length = 314 residues,  96.5% aligned
Score =  209 bits (532), Expect = 3e-55
Identities = 124/310 (40%), Positives = 155/310 (50%), Gaps = 15/310 (4%)

COG1087, GalE, UDP-glucose 4-epimerase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 329 residues,  89.4% aligned
Score =  204 bits (521), Expect = 9e-54
Identities = 106/299 (35%), Positives = 150/299 (50%), Gaps = 23/299 (7%)

COG1088, RfbB, dTDP-D-glucose 4,6-dehydratase 
[Cell envelope biogenesis, outer membrane]
CD-Length = 340 residues,  91.5% aligned
Score =  162 bits (412), Expect = 4e-41
Identities = 101/318 (31%), Positives = 147/318 (46%), Gaps = 23/318 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4889




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22035




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36524




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2443_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2443
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4100
                           PA4068_tr
                           translation of PA4068
                           309
                           99.67
                           99.67
                           0
                           1558.0
     

     
     
122644
                           gb|AAG07455.1|AE004823_1
                           (AE004823) probable epimerase [Pseudomonas aeruginosa PAO1]
                           309
                           99.67
                           99.67
                           0
                           1558.0
     

     
     
122645
                           gb|AAN66129.1|AE016241_6
                           (AE016775) NAD-dependent epimerase/dehydratase family protein [Pseudomonas putida KT2440]
                           310
                           71.29
                           82.90
                           1
                           1147.0
     

     
     
122646
                           gb|AAO54220.1|
                           (AE016858) NAD-dependent epimerase/dehydratase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           309
                           69.57
                           79.93
                           0
                           1116.0
     

     
     
122647
                           gb|AAM07805.1|
                           (AE011167) dTDP-glucose 4,6-dehydratase [Methanosarcina acetivorans str. C2A] [Methanosarcina acetivorans C2A]
                           310
                           40.96
                           58.06
                           13
                           569.0
     

     






      

          
GC ORFID: 45445How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4068Glimmer Score: 

          
Start: 3974136Stop: 3973207

          
Length: 930


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4068 translation of PA4068Homolog Bit Score 604.0

          
Other Homologs:  t_PA4068 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTTTGAACGCCCCATCCTGATCACTGGCGGCGCCGGTTTCATCGGCTCCCACCTGACCGACGCGCTGCTG 
GCGCGCGGCTACCGCGTGCGTGTGCTGGACAACCTGTCCACCGGCAAGCGCGACAACCTGCCCCTGGACGAC 
GAACGGGTAGAGCTGCTCGAAGGCGACGCCGCCGACGCGGCGACCCTGGCGGCCGCCCTGAAGGGCTGCGCG 
GCGGTCGCCCACCTGGCCGCGGTCGCCTCGGTACAGGCCTCGGTGGACGACCCGGTCGCCACCCACCAGAGC 
AATTTCATCGCCACCCTGAACCTCTGCGAAGCGATGCGCGAGCAGGGCGTGAAGCGCGTGGTGTTCGCTTCC 
AGCGCAGCGGTCTACGGGCAGAACGGCGAAGGCACGGCGATCGACGAAGACACCCCGAAGTCGCCGCTGACG 
CCCTACGCGTCGGACAAGCTGGCCAGCGAGTACTACCTCGATTTCTATCGCCGCCAGCATGGGCTGGAACCG 
GCGATCTTCCGCTTCTTCAACGTCTACGGGCCGCGCCAGGACCCGTCCTCGCCCTATTCCGGGGTGATCAGC 
ATATTCACCGAGCGCGCCCTGAAGGGGACGCCGATCACCGTGTTCGGCGACGGCGAGCAGACCCGCGACTTC 
ATCTACGTCGCCGACCTGGTCGATTTCCTGGTCCAGGCGCTGGAGGCGACGGCGGTGGAGCCCGGGGCGGTG 
AACGTCGGCCTGAATCGCAGCATCAGCCTGAAGCAGTTGCTGGCGGAGATCGGCCAGGTGCTCGGTGGCCTG 
CCGCCGGTGACCCACGCCGACGCGCGAGCCGGTGACATCAGGCATTCGCGGGCGAACAACGAGCGCCTGCTG 
CGGCGTTTCTGCTTCGACCAGCCGACGCCGATGGGCGTGGGCCTGGCGCGCCTGCTCGGCCGCTGA