ViewGene
Locus Name:
GeneID:
Location:

GeneID:244
TrivialName: PA14_66720
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66720
PAO1 Ortholog Locus:PA5050
Sequence Length:2220
Protein Length:739
Start: 5958880
Stop: 5961099
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08435.1|AE004918_5 (AE004918) primosomal protein N' [Pseudomonas aeruginosa PAO1]
Identities = 737/739 (99%), Positives = 738/739 (99%)
Sequence:
GTGTCAGACCTGATCCTACGCCTAGCCCTGCCTTCGCCACTCCGCCGCCTGTTCGACTACCGCGCGCCCCGC
GGCATTCCGCGCAGCGCCCTACAGCCGGGGATTCGCCTGCTGCTGCCGTTCGGGCGCCGGGAGCTGGTTGGC
GTTCTGATAGAGGTGACGGACCGTTCCGAAGTACCCGAAGACAAGCTCAAGCCTGCCCTCAGGGTACTCGAC
GCCAAGCCGCCAATGCCGGCGCACCTGCTCGAACTCTGTCGCTGGACCGCCCAGTACTACCAGCACAGCCTC
GGCGACACCCTGTCCTGGGCGCTGCCCAACCTGTTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGCAGCGC
TTCTGGCATGCCGCCGCGCAATCCAGCCTCGACGATCCGCGCCTGGCCCGGGCGCCGCGCCAGCGCCAGGCC
CTGGCGATTCTCAAGCAGCATCCGCACGGGGTTTCCCATGAGTTGCTGAACCAATTGGAAATAAACAAGGAC
AGCCTCGACCTGCTCAAGGAAAAAGGCCTGGTCGAACTGGAGGTGCGGCGCCACAGCACCCCGCCGCGCGAA
GGCGGCTGGCTGGCCCAGGCGGAGTTGCCGCTGAACCCCGAGCAGCGCGCCGCCTTCGAAGCGGTTCGCGCC
AGCCACGGCGGTTTCCATTGCTTCCTGCTGGCCGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA
CTGATCCGCGAAACCCTGGCCGCAggCCGCCAGGCCCTGGTGCTGATTCCCGAGATCAACCTGGgCCCGCAG
ACCCTGGCGCGTTTCGAACGCCGCTTCAACGCGCGCATCGCCCTGCTGCATTCGGCCCTGACCGACCGCGAG
CGCCTCGACGCCTGGCTCGCCGCCCGGGATGGCGAGGCCGACATCGTCATCGGCACCCGTTCGGCACTGTTC
ACGCCGCTGAAGCGGCCGGGACTGATCATCGTCGACGAGGAACACGACGCCTCCTATAAACAGCAGGACGGC
CTGCGCTACCACGCCCGCGACCTGGCACTGGTACGCGCGCGCCTGGAGAACGTGCCGATCCTGCTCGGTTCG
GCCACGCCCGCCCTGGAAAGCCTGCACAACGCCCAGGCCGGCCGCTACGGCCTGCTGCGCCTGACCCAGCGC
GCCGGTGGCGCGCACCCGCCGAAGTTCATCCGCCTCGACGTGAAGAGCATGCCGCTCGACGCCGGCCTTTCC
CGCCCGCTGCAACAGGCCATCGGCGATACCCTGGCGGCCGGCCAGCAGGTCCTGGTGTTCCTCAACCGCCGC
GGCTTCGCTCCGACCCTGCTTTGCCACGACTGCGGCTGGATCAGCCAATGCCCGCGCTGCGACGCGCGGATG
ACCGTGCACCAGGGCAGCGGCGAGTTGCGCTGCCACCACTGCGACCACCGCCAGCGGCCGCCGATGAACTGC
CCGCAATGCGGCAAGCTCGACCTGCGTCCGGTAGGCGCCGGCACCGAGCGCGCCGAGGAACGCCTGCGGATT
CTCTTCCCGAACCATCCGGTGCTGCGCATCGACCGCGACAGCACCTCGCGCAAGCACGCCATGCGCGACCTC
TTCGCCACCATCAACAGCGGTGAGCCATGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGGCACCACTTC
CCACGGGTCACCCTGGTGGCGATCCTCGATGCCGATGGCGGCCTGTTTTCCGCCGACTTCCGCGCCAGCGAA
CGCATGGCCCAGCAGATCGTCCAGGTCGCCGGCCGCGCAGGACGCGCCGAGGAGCCCGGGCGGGTACTGATC
CAGACCCACCTGGCCGATCACCCGCTGCTGGTCCAGCTCACCGAGGACGGCTACTTCGCCTTTGCCGAACAG
GCCCTCAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACCTCGCCCTGCTGCGCGCCGAAGCGCAC
AAGCCGGGCCAGGCGGAAGCCTTCCTCGACAGCGCCTGCAGTGCCGCCGAGCAATTGCTCGAGCAGATGGGC
GGACCGGAGGTGGAACTGCTCGGGCCGGTGCCCGCGCCCATGGAGCGGCGTGCCGGCAAGCACCGCGCGCAA
CTGCTGCTGCAGTGCATGAGCCGGGCGCCGCTGCATCGCCTGCTGACGCCCTGGCTGCAGAGCCTCGAACAG
TTGCCCGGCGGGCGCCAGGTGCGCTGGTCGCTCGATATCGATCCGATCGACCTGTTCTGA
Translation:
MSDLILRLALPSPLRRLFDYRAPRGIPRSALQPGIRLLLPFGRRELVGVLIEVTDRSEVPEDKLKPALRVLD
AKPPMPAHLLELCRWTAQYYQHSLGDTLSWALPNLLRQGEPAEARQQRFWHAAAQSSLDDPRLARAPRQRQA
LAILKQHPHGVSHELLNQLEINKDSLDLLKEKGLVELEVRRHSTPPREGGWLAQAELPLNPEQRAAFEAVRA
SHGGFHCFLLAGVTGSGKTEVYLQLIRETLAAGRQALVLIPEINLGPQTLARFERRFNARIALLHSALTDRE
RLDAWLAARDGEADIVIGTRSALFTPLKRPGLIIVDEEHDASYKQQDGLRYHARDLALVRARLENVPILLGS
ATPALESLHNAQAGRYGLLRLTQRAGGAHPPKFIRLDVKSMPLDAGLSRPLQQAIGDTLAAGQQVLVFLNRR
GFAPTLLCHDCGWISQCPRCDARMTVHQGSGELRCHHCDHRQRPPMNCPQCGKLDLRPVGAGTERAEERLRI
LFPNHPVLRIDRDSTSRKHAMRDLFATINSGEPCILVGTQMLAKGHHFPRVTLVAILDADGGLFSADFRASE
RMAQQIVQVAGRAGRAEEPGRVLIQTHLADHPLLVQLTEDGYFAFAEQALSERRAAGLPPFAHLALLRAEAH
KPGQAEAFLDSACSAAEQLLEQMGGPEVELLGPVPAPMERRAGKHRAQLLLQCMSRAPLHRLLTPWLQSLEQ
LPGGRQVRWSLDIDPIDLF*
AnnotationID:254GeneID:244
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 11:11:07
Gene Name:priA
Confidence Code:2
GeneProduct:primosomal protein N'
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:replication factor Y
Functional Category Confidence Code:5
COGs:COG1198
Secondary Functional Category(ies):
EC Number:5.3.1.16, 5.3.1.24
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO58564.1| (AE016874) primosomal protein N` [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 739

 Score = 1191 bits (3082), Expect = 0.0
 Identities = 581/739 (78%), Positives = 653/739 (88%)

>gb|AAN70653.1|AE016709_5 (AE016793) primosomal protein N` [Pseudomonas putida KT2440]
          Length = 739

 Score = 1160 bits (3002), Expect = 0.0
 Identities = 569/739 (76%), Positives = 639/739 (86%)

>gb|AAM83890.1|AE013629_1 (AE013629) primosomal protein N' [Yersinia pestis KIM]
          Length = 732

 Score =  691 bits (1784), Expect = 0.0
 Identities = 362/735 (49%), Positives = 479/735 (65%), Gaps = 8/735 (1%)








 
Structural Features:
COG1198, PriA, Primosomal protein N' (replication factor Y) - superfamily II helicase 
[DNA replication, recombination, and repair]
CD-Length = 730 residues, 100.0% aligned
Score =  865 bits (2237), Expect = 0.0
Identities = 364/738 (49%), Positives = 473/738 (64%), Gaps = 10/738 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:14093




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17633




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35017




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:244_cdd.html


    		




 
URL:

















    ReferenceID:35838




  
Author/Investigator(s):
        Nurse P, DiGate RJ, Zavitz KH, Marians KJ.





  
Title:
        Molecular cloning and DNA sequence analysis of Escherichia coli priA, the gene encoding the primosomal protein replication factor Y.




  
PubMed:
        2162049


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1990 Jun;87(12):4615-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2162049

















    ReferenceID:35839




  
Author/Investigator(s):
        Lee EH, Masai H, Allen GC Jr, Kornberg A.





  
Title:
        The priA gene encoding the primosomal replicative n' protein of Escherichia coli.




  
PubMed:
        2162050


MedLine:
        




    
Source:
Proc Natl Acad Sci U S A. 1990 Jun;87(12):4620-4.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2162050

















    ReferenceID:35840




  
Author/Investigator(s):
        Kajiwara S, Yamaoka K, Hori K, Miyazawa H, Saito T, Kanno T, Shishido K.





  
Title:
        Isolation and sequence of a developmentally regulated putative novel gene, priA, from the basidiomycete Lentinus edodes.




  
PubMed:
        1601301


MedLine:
        




    
Source:
Gene. 1992 May 15;114(2):173-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1601301









           
Homologs By Global Alignment 

           

       



     
Gene ID:244
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
414
                           PA5050_tr
                           translation of PA5050
                           739
                           99.72
                           99.86
                           0
                           3831.0
     

     
     
22974
                           gb|AAG08435.1|AE004918_5
                           (AE004918) primosomal protein N' [Pseudomonas aeruginosa PAO1]
                           739
                           99.59
                           99.86
                           0
                           3828.0
     

     
     
22975
                           gb|AAO58564.1|
                           (AE016874) primosomal protein N` [Pseudomonas syringae pv. tomato str. DC3000]
                           741
                           78.54
                           88.39
                           4
                           3079.0
     

     
     
22976
                           gb|AAN70653.1|AE016709_5
                           (AE016793) primosomal protein N` [Pseudomonas putida KT2440]
                           739
                           76.86
                           86.46
                           0
                           2998.0
     

     
     
22991
                           gb|AAF95819.1|
                           (AE004333) primosomal protein N` [Vibrio cholerae O1 biovar eltor str. N16961]
                           759
                           50.72
                           64.03
                           45
                           1785.0
     

     






      

          
GC ORFID: 43246How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5050Glimmer Score: 

          
Start: 5222563Stop: 5224782

          
Length: 2220


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5050 translation of PA5050Homolog Bit Score 1480.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3786 (ORF: BLASTX 1186.0), t_PA5050 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGTCAGACCTGATCCTACGCCTAGCCCTGCCTTCGCCACTCCGCCGCCTGTTCGACTACCGCGCGCCCCGC 
GGCATTCCGCGCAGCGCCCTACAGCCGGGGATTCGCCTGCTGCTGCCGTTCGGGCGCCGGGAGCTGGTTGGC 
GTTCTGATAGAGGTGACGGACCGTTCCGAAGTACCCGAAGACAAGCTCAAGCCTGCCCTCAGGGTACTCGAC 
GCCAAGCCGCCAATGCCGGCGCACCTGCTCGAACTCTGTCGCTGGACCGCCCAGTACTACCAGCACAGCCTC 
GGCGACACCCTGTCCTGGGCGCTGCCCAACCTGTTGCGCCAGGGCGAGCCGGCCGAGGCGCGCCAGCAGCGC 
TTCTGGCATGCCGCCGCGCAATCCAGCCTCGACGATCCGCGCCTGGCCCGGGCGCCGCGCCAGCGCCAGGCC 
CTGGCGATTCTCAAGCAGCATCCGCACGGGGTTTCCCATGAGTTGCTGAACCAATTGGAAATAAACAAGGAC 
AGCCTCGACCTGCTCAAGGAAAAAGGCCTGGTCGAACTGGAGGTGCGGCGCCACAGCACCCCGCCGCGCGAA 
GGCGGCTGGCTGGCCCAGGCGGAGTTGCCGCTGAACCCCGAGCAGCGCGCCGCCTTCGAAGCGGTTCGCGCC 
AGCCACGGCGGTTTCCATTGCTTCCTGCTGGCCGGGGTCACCGGCAGCGGCAAGACCGAGGTCTACCTGCAA 
CTGATCCGCGAAACCCTGGCCGCAggCCGCCAGGCCCTGGTGCTGATTCCCGAGATCAACCTGGgCCCGCAG 
ACCCTGGCGCGTTTCGAACGCCGCTTCAACGCGCGCATCGCCCTGCTGCATTCGGCCCTGACCGACCGCGAG 
CGCCTCGACGCCTGGCTCGCCGCCCGGGATGGCGAGGCCGACATCGTCATCGGCACCCGTTCGGCACTGTTC 
ACGCCGCTGAAGCGGCCGGGACTGATCATCGTCGACGAGGAACACGACGCCTCCTATAAACAGCAGGACGGC 
CTGCGCTACCACGCCCGCGACCTGGCACTGGTACGCGCGCGCCTGGAGAACGTGCCGATCCTGCTCGGTTCG 
GCCACGCCCGCCCTGGAAAGCCTGCACAACGCCCAGGCCGGCCGCTACGGCCTGCTGCGCCTGACCCAGCGC 
GCCGGTGGCGCGCACCCGCCGAAGTTCATCCGCCTCGACGTGAAGAGCATGCCGCTCGACGCCGGCCTTTCC 
CGCCCGCTGCAACAGGCCATCGGCGATACCCTGGCGGCCGGCCAGCAGGTCCTGGTGTTCCTCAACCGCCGC 
GGCTTCGCTCCGACCCTGCTTTGCCACGACTGCGGCTGGATCAGCCAATGCCCGCGCTGCGACGCGCGGATG 
ACCGTGCACCAGGGCAGCGGCGAGTTGCGCTGCCACCACTGCGACCACCGCCAGCGGCCGCCGATGAACTGC 
CCGCAATGCGGCAAGCTCGACCTGCGTCCGGTAGGCGCCGGCACCGAGCGCGCCGAGGAACGCCTGCGGATT 
CTCTTCCCGAACCATCCGGTGCTGCGCATCGACCGCGACAGCACCTCGCGCAAGCACGCCATGCGCGACCTC 
TTCGCCACCATCAACAGCGGTGAGCCATGCATCCTGGTCGGCACCCAGATGCTCGCCAAGGGGCACCACTTC 
CCACGGGTCACCCTGGTGGCGATCCTCGATGCCGATGGCGGCCTGTTTTCCGCCGACTTCCGCGCCAGCGAA 
CGCATGGCCCAGCAGATCGTCCAGGTCGCCGGCCGCGCAGGACGCGCCGAGGAGCCCGGGCGGGTACTGATC 
CAGACCCACCTGGCCGATCACCCGCTGCTGGTCCAGCTCACCGAGGACGGCTACTTCGCCTTTGCCGAACAG 
GCCCTCAGCGAACGCCGCGCCGCCGGCCTGCCGCCCTTCGCCCACCTCGCCCTGCTGCGCGCCGAAGCGCAC 
AAGCCGGGCCAGGCGGAAGCCTTCCTCGACAGCGCCTGCAGTGCCGCCGAGCAATTGCTCGAGCAGATGGGC 
GGACCGGAGGTGGAACTGCTCGGGCCGGTGCCCGCGCCCATGGAGCGGCGTGCCGGCAAGCACCGCGCGCAA 
CTGCTGCTGCAGTGCATGAGCCGGGCGCCGCTGCATCGCCTGCTGACGCCCTGGCTGCAGAGCCTCGAACAG 
TTGCCCGGCGGGCGCCAGGTGCGCTGGTCGCTCGATATCGATCCGATCGACCTGTTCTGA