ViewGene
Locus Name:
GeneID:
Location:

GeneID:2434
TrivialName: PA14_57160
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57160
PAO1 Ortholog Locus:PA4397
Sequence Length:912
Protein Length:303
Start: 5090592
Stop: 5091503
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07785.1|AE004855_7 (AE004855) ketopantoate reductase [Pseudomonas aeruginosa PAO1]
Identities = 299/303 (98%), Positives = 301/303 (99%)
Sequence:
ATGACCTGGCATATCCTCGGCGCCGGTAGCCTCGGCAGCCTCTGGGCCGCGCGCCTCGGGCGCGCCGGCCTG
CCCGTCCGCCTGATCCTGCGCGACCGCCAGCGCCTGCGCCGCTACCAACAGGCCGGCGGACTGAACCTGGTG
GAAGACGGCCAGGCCAGCCTCTACCCGATCGCCGCGGAAACCCCGGACGGCGGGCAACCCATCCAGCGCCTG
CTGCTGGCCTGCAAGGCCTACGACGCGGAGGAAGCCGCCTCCAGCGTGGCCCATCGCCTGGCCGGCAACGCC
GAACTGCTGTTGCTGCAGAACGGCCTGGGCAGCCAGCAGGCGGTCGCCGCCCGCCTGCCGCGCAGCCGCTGC
CTGTTCGCCTCCAGCACCGAAGGCGCGTTCCGCGATGGCGATTTCCGCGTGGTCTTCGCCGGCCGCGGGCAC
ACCTGGCTCGGCGATCCGCGCGACACAAGCGCCCCCGCCTGGCTGGCGCAGCTGTCCCAGGCCGGGATCCCG
CACAGCTGGAGCGACGACATCCTCGAACGCCTGTGGCGCAAGCTGGCCCTGAACTGCGCGATCAACCCGCTG
ACCGTCCTCCACGATTGCCACAACGGCGGCCTGCGGCAGCACCCGGAAGAGATCGCCGCGCTTTGCGACGAA
CTCGGCCAGTTGCTCCACGCCAGCGGCTACGACGCCGCCGCCAGGAGCCTGCTGGAGGATGTCCGGGCGGTG
ATCGACGCCACCGCGGCCAACTATTCCTCGATGCATCAGGACGTCACCCGCGGCCGGCGCACGGAAATCGGC
TACCTGCTCGGCTACGCCTGCCAGCACGGGCAGCGCCTGGGACTACCGTTGCCGCGCCTAGGAACCTTGCTC
GCCCGCCTCCAGGCCCATCTGCGCCAGCGCGGTTTGCCCGACCGCTAA
Translation:
MTWHILGAGSLGSLWAARLGRAGLPVRLILRDRQRLRRYQQAGGLNLVEDGQASLYPIAAETPDGGQPIQRL
LLACKAYDAEEAASSVAHRLAGNAELLLLQNGLGSQQAVAARLPRSRCLFASSTEGAFRDGDFRVVFAGRGH
TWLGDPRDTSAPAWLAQLSQAGIPHSWSDDILERLWRKLALNCAINPLTVLHDCHNGGLRQHPEEIAALCDE
LGQLLHASGYDAAARSLLEDVRAVIDATAANYSSMHQDVTRGRRTEIGYLLGYACQHGQRLGLPLPRLGTLL
ARLQAHLRQRGLPDR*
AnnotationID:2442GeneID:2434
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 13:01:37
Gene Name:apbA
Confidence Code:2
GeneProduct:Ketopantoate reductase
Cell Localization:()
Synonyms:panE
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:2-dehydropantoate 2-reductase
Functional Category Confidence Code:5
COGs:COG1893,pfam02558
Secondary Functional Category(ies):nucleotide biosynthesis and metabolism
EC Number:1.1.1.169
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN66974.1|AE016325_10 (AE016779) 2-dehydropantoate 2-reductase, putative [Pseudomonas
           putida KT2440]
          Length = 305

 Score =  376 bits (965), Expect = e-103
 Identities = 197/300 (65%), Positives = 221/300 (73%)

>gb|AAO57843.1| (AE016871) 2-dehydropantoate 2-reductase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 306

 Score =  365 bits (938), Expect = e-100
 Identities = 190/299 (63%), Positives = 217/299 (72%), Gaps = 1/299 (0%)








 
Structural Features:
COG1893, ApbA, Ketopantoate reductase [Coenzyme metabolism]
CD-Length = 307 residues, 100.0% aligned
Score =  248 bits (634), Expect = 1e-66
Identities = 116/308 (37%), Positives = 163/308 (52%), Gaps = 7/308 (2%)

pfam02558, ApbA, Ketopantoate reductase PanE/ApbA
CD-Length = 257 residues,  95.3% aligned
Score =  167 bits (425), Expect = 3e-42
Identities = 84/245 (34%), Positives = 114/245 (46%), Gaps = 7/245 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4871




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22017




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:28130




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2434_cdd.html


    		




 
URL:

















    ReferenceID:38115




  
Author/Investigator(s):
        Downs DM, Petersen L.





  
Title:
        apbA, a new genetic locus involved in thiamine biosynthesis in Salmonella typhimurium.




  
PubMed:
        7519593


MedLine:
        




    
Source:
J Bacteriol. 1994 Aug;176(16):4858-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=7519593









           
Homologs By Global Alignment 

           

       



     
Gene ID:2434
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4089
                           PA4397_tr
                           translation of PA4397
                           303
                           98.67
                           99.33
                           0
                           1559.0
     

     
     
122349
                           gb|AAG07785.1|AE004855_7
                           (AE004855) ketopantoate reductase [Pseudomonas aeruginosa PAO1]
                           303
                           98.67
                           99.33
                           0
                           1559.0
     

     
     
122350
                           gb|AAN66974.1|AE016325_10
                           (AE016779) 2-dehydropantoate 2-reductase, putative [Pseudomonas putida KT2440]
                           306
                           64.70
                           72.54
                           4
                           960.0
     

     
     
122351
                           gb|AAO57843.1|
                           (AE016871) 2-dehydropantoate 2-reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           306
                           62.09
                           71.24
                           3
                           935.0
     

     






      

          
GC ORFID: 45436How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4397Glimmer Score: 

          
Start: 4354268Stop: 4355179

          
Length: 912


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA4397 translation of PA4397Homolog Bit Score 605.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene315 (ORF: BLASTX 367.0), t_PA4397 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGACCTGGCATATCCTCGGCGCCGGTAGCCTCGGCAGCCTCTGGGCCGCGCGCCTCGGGCGCGCCGGCCTG 
CCCGTCCGCCTGATCCTGCGCGACCGCCAGCGCCTGCGCCGCTACCAACAGGCCGGCGGACTGAACCTGGTG 
GAAGACGGCCAGGCCAGCCTCTACCCGATCGCCGCGGAAACCCCGGACGGCGGGCAACCCATCCAGCGCCTG 
CTGCTGGCCTGCAAGGCCTACGACGCGGAGGAAGCCGCCTCCAGCGTGGCCCATCGCCTGGCCGGCAACGCC 
GAACTGCTGTTGCTGCAGAACGGCCTGGGCAGCCAGCAGGCGGTCGCCGCCCGCCTGCCGCGCAGCCGCTGC 
CTGTTCGCCTCCAGCACCGAAGGCGCGTTCCGCGATGGCGATTTCCGCGTGGTCTTCGCCGGCCGCGGGCAC 
ACCTGGCTCGGCGATCCGCGCGACACAAGCGCCCCCGCCTGGCTGGCGCAGCTGTCCCAGGCCGGGATCCCG 
CACAGCTGGAGCGACGACATCCTCGAACGCCTGTGGCGCAAGCTGGCCCTGAACTGCGCGATCAACCCGCTG 
ACCGTCCTCCACGATTGCCACAACGGCGGCCTGCGGCAGCACCCGGAAGAGATCGCCGCGCTTTGCGACGAA 
CTCGGCCAGTTGCTCCACGCCAGCGGCTACGACGCCGCCGCCAGGAGCCTGCTGGAGGATGTCCGGGCGGTG 
ATCGACGCCACCGCGGCCAACTATTCCTCGATGCATCAGGACGTCACCCGCGGCCGGCGCACGGAAATCGGC 
TACCTGCTCGGCTACGCCTGCCAGCACGGGCAGCGCCTGGGACTACCGTTGCCGCGCCTAGGAACCTTGCTC 
GCCCGCCTCCAGGCCCATCTGCGCCAGCGCGGTTTGCCCGACCGCTAA