ViewGene
Locus Name:
GeneID:
Location:

GeneID:2429
TrivialName: PA14_37530
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_37530
PAO1 Ortholog Locus:PA2086
Sequence Length:903
Protein Length:300
Start: 3340853
Stop: 3339951
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05474.1|AE004636_5 (AE004636) probable epoxide hydrolase [Pseudomonas aeruginosa PAO1]
Identities = 295/300 (98%), Positives = 296/300 (98%)
Sequence:
ATGAGCACCGATCCGTTGCTGTCCGGCTTCGACTACCTGACCCTGCACACCTCCGCCGCGCGCCTCCGCGTC
GCCGTGAAGGGCAGCGGCCCGCCGCTGCTGCTGTTGCATGGCTATCCGCAGACCCACCTGGCCTGGCACCGC
ATCGCTCCGCGCCTGGCCGAGGACTACAGCGTGGTCCTCGCCGACCTGCGCGGCTACGGCGAATCGCGGGCG
CTGGACGAGGAGGGCGCCGACTATTCCAAGGCCGCGCTGGCCCGCGACCAACTGGAAACGATGGGCCAGCTC
GGCTTCGAACGCTTCACGGTGATCGGCCACGATCGCGGCGCGCGGGTCGGCTATCGCCTGGCGCTGGACCAT
CCGCAGGCGGTCGCGGCCTTCGTCTCGCTGACCGTGGTGCCGATCCTCGACAACTGGGCCGCGGTGAACAAG
GCCTTCGCCCTCAACGCCTACCACTGGTTCCTCCTCGCCCAGCCGTACGACCTGCCGGAGCGCCTGATCGGC
GCCGATCCGGAGCACTTCCTCGACTACACCCTGCGACGCATGGCCCAGGGCCGCGACATCTACCACCCGCAG
GCGCTGGCAAGCTACCGCCGGGCGTTCCGCGACCCCGCCGTGCGCCATGCGATGTGCGAGGACTACCGGGCC
GCGGTGGGCGTCGATGCCGACGCCGACCAGGCCGATCGCGACGCCGGCCGGCGCCTGCAATGCCCGGTCCAG
GTGCTCTGGCAGGAACGCCCCTACGCCGCCGGGCAGCATCCGCTGGAAATCTGGAAGACCTGGGCCGGGCAG
GTCGAGGGCGCCGCCATCGGCGCCAGCCACATGCTCCCCGAAGACGCGCCGGACGCGGTGCTGGAGCACCTG
CTGGGGTTTCTCGCCAGCCACCGGGAGGCGCTCCGATGA
Translation:
MSTDPLLSGFDYLTLHTSAARLRVAVKGSGPPLLLLHGYPQTHLAWHRIAPRLAEDYSVVLADLRGYGESRA
LDEEGADYSKAALARDQLETMGQLGFERFTVIGHDRGARVGYRLALDHPQAVAAFVSLTVVPILDNWAAVNK
AFALNAYHWFLLAQPYDLPERLIGADPEHFLDYTLRRMAQGRDIYHPQALASYRRAFRDPAVRHAMCEDYRA
AVGVDADADQADRDAGRRLQCPVQVLWQERPYAAGQHPLEIWKTWAGQVEGAAIGASHMLPEDAPDAVLEHL
LGFLASHREALR*
AnnotationID:2437GeneID:2429
AnnotatorUID: diggins
Modification Date/Time: 2005-03-03 07:07:48
Gene Name:
Confidence Code:3
GeneProduct:putative hydrolase
Cell Localization:()
Synonyms:PA2086
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0596,pfam00561
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL45985.1| (AE008952) hydrolase [Agrobacterium tumefaciens str. C58 (U.
           Washington)]
          Length = 307

 Score =  275 bits (704), Expect = 2e-73
 Identities = 138/269 (51%), Positives = 177/269 (65%), Gaps = 2/269 (0%)

>dbj|BAB49078.1| (AP002998) probable epoxide hydrolase [Mesorhizobium loti]
          Length = 300

 Score =  228 bits (581), Expect = 3e-59
 Identities = 122/289 (42%), Positives = 157/289 (54%), Gaps = 7/289 (2%)

>emb|CAB62690.1| (AL939107) putative hydrolase. [Streptomyces coelicolor A3(2)]
          Length = 298

 Score =  216 bits (551), Expect = 1e-55
 Identities = 116/285 (40%), Positives = 164/285 (57%), Gaps = 4/285 (1%)






 
Structural Features:
COG0596, MhpC, Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)
CD-Length = 282 residues,  94.7% aligned
Score = 80.9 bits (197), Expect = 2e-16
Identities = 76/276 (27%), Positives = 99/276 (35%), Gaps = 14/276 (5%)

pfam00561, Abhydrolase_1, alpha/beta hydrolase fold. 
CD-Length = 224 residues,  98.2% aligned
Score = 66.0 bits (160), Expect = 6e-12
Identities = 45/231 (19%), Positives = 74/231 (32%), Gaps = 12/231 (5%)







   
Genomic Context:






   
Comment:


















    ReferenceID:4861




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:22007




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32620




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2429_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2429
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4084
                           PA2086_tr
                           translation of PA2086
                           300
                           98.33
                           98.66
                           0
                           1560.0
     

     
     
122321
                           gb|AAG05474.1|AE004636_5
                           (AE004636) probable epoxide hydrolase [Pseudomonas aeruginosa PAO1]
                           300
                           98.33
                           98.66
                           0
                           1560.0
     

     
     
122322
                           gb|AAL45985.1|
                           (AE008952) hydrolase [Agrobacterium tumefaciens str. C58 (U. Washington)]
                           308
                           45.45
                           59.41
                           9
                           696.0
     

     
     
122323
                           gb|AAK90672.1|
                           (AE007899) AGR_pAT_427p [Agrobacterium tumefaciens str. C58 (Cereon)]
                           308
                           45.45
                           59.41
                           9
                           696.0
     

     
     
122324
                           emb|CAE26606.1|
                           (BX572596) possible epoxide hydrolase [Rhodopseudomonas palustris CGA009]
                           309
                           43.04
                           59.22
                           16
                           678.0
     

     






      

          
GC ORFID: 45431How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2086Glimmer Score: 

          
Start: 1613061Stop: 1613963

          
Length: 903


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2086 translation of PA2086Homolog Bit Score 605.0

          
Other Homologs:  t_PA2086 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCACCGATCCGTTGCTGTCCGGCTTCGACTACCTGACCCTGCACACCTCCGCCGCGCGCCTCCGCGTC 
GCCGTGAAGGGCAGCGGCCCGCCGCTGCTGCTGTTGCATGGCTATCCGCAGACCCACCTGGCCTGGCACCGC 
ATCGCTCCGCGCCTGGCCGAGGACTACAGCGTGGTCCTCGCCGACCTGCGCGGCTACGGCGAATCGCGGGCG 
CTGGACGAGGAGGGCGCCGACTATTCCAAGGCCGCGCTGGCCCGCGACCAACTGGAAACGATGGGCCAGCTC 
GGCTTCGAACGCTTCACGGTGATCGGCCACGATCGCGGCGCGCGGGTCGGCTATCGCCTGGCGCTGGACCAT 
CCGCAGGCGGTCGCGGCCTTCGTCTCGCTGACCGTGGTGCCGATCCTCGACAACTGGGCCGCGGTGAACAAG 
GCCTTCGCCCTCAACGCCTACCACTGGTTCCTCCTCGCCCAGCCGTACGACCTGCCGGAGCGCCTGATCGGC 
GCCGATCCGGAGCACTTCCTCGACTACACCCTGCGACGCATGGCCCAGGGCCGCGACATCTACCACCCGCAG 
GCGCTGGCAAGCTACCGCCGGGCGTTCCGCGACCCCGCCGTGCGCCATGCGATGTGCGAGGACTACCGGGCC 
GCGGTGGGCGTCGATGCCGACGCCGACCAGGCCGATCGCGACGCCGGCCGGCGCCTGCAATGCCCGGTCCAG 
GTGCTCTGGCAGGAACGCCCCTACGCCGCCGGGCAGCATCCGCTGGAAATCTGGAAGACCTGGGCCGGGCAG 
GTCGAGGGCGCCGCCATCGGCGCCAGCCACATGCTCCCCGAAGACGCGCCGGACGCGGTGCTGGAGCACCTG 
CTGGGGTTTCTCGCCAGCCACCGGGAGGCGCTCCGATGA