ViewGene
Locus Name:
GeneID:
Location:

GeneID:2419
TrivialName: PA14_02450
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_02450
PAO1 Ortholog Locus:
Sequence Length:1119
Protein Length:372
Start: 220837
Stop: 221955
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAN62244.1|AF440524_31 (AF440524) putative proton-translocating NAD(P) transhydrogenase [Pseudomonas aeruginosa]
Identities = 328/373 (87%), Positives = 350/373 (93%), Gaps = 1/373 (0%)
Sequence:
GTGCAGATCGGTGTCCCCCTCGAGACCCATGCCGGTGAGACGCGGGTTTCCGCCACCCCGGAAACCGTCAAG
AAGCTGATCGGCCAAGGCCACCAGGTGATCGTCCAGAGCGGCGCCGGCGTCAACGCCAGCCAGCCGGACAGT
GCCTATGAAGCCGCCGGCGCGACGATCGGCAGCGCCGCCGAGGCCTTCGGCGCCGACCTGGTGCTGAAGGTG
GTCGCGCCCAGCGCCGCCGAGCTGGCCCAGATGAAAAGCGGTGCCGTGCTGGTCGGCATGCTCAACCCCTTC
GACAACGAGAACATCGCCCGCATGGCCGAGCGCGGCATCACCGCCTTCGCCCTCGAGGCCGCGCCGCGTACC
TCGCGGGCGCAGAGCCTGGATGTGCTCTCCTCGCAGGCCAACATCGCCGGCTACAAGGCGGTGATGCTCGCC
GCCAACCACTACCCGCGCTTCATGCCGATGCTGATGACCGCCGCCGGCACCGTGAAGGCCGCCCGCGTGCTG
ATCCTCGGCGCCGGCGTCGCCGGCCTGCAGGCGATCGCCACGGCCAAGCGCCTGGGCGCGGTGATCGAAGCT
TCCGACGTGCGTCCGGCGGTGAAGGAGCAGATCGAGTCGCTCGGCGCCAAGTTCGTCGACGTGCCCTACGAG
ACCGACGAAGAGCGCGAGTGCGCCGAAGGCGTCGGCGGCTATGCGCGGCCGATGCCGGCCTCGTGGATGGAG
CGCCAGGCCAAGGCCGTGCACGAGCGCGCCAAGCAGTCGGACATCGTCATCACTACCGCGCTGATCCCCGGC
CGCAAGGCGCCGACGCTGCTGCACGAGGCGACCGTGGCGGAGATGAAGCCGGGCTCGGTGGTCATCGACCTG
GCCGCCGCCCAGGGCGGCAACTGCCCGCTGACCGAGGCCGACCAGGTCGTGGTCCGGCACGGCGTGACCATC
GTCGGCCACAGCAACCTGGCCGCGCTGGTACCGGCGGACGCCTCGGCGCTATACGCGCGCAACATCCTCGAC
TTCCTCAAGCTCACCCTGAACGCCGAAGGCTTCAGCGTGAACCTCGAGGACGACATCGTCGCCGCCTGCCTG
ATGTGCCGCGACGGCCAGGCGGTGCGCAAGAACGGCTGA
Translation:
MQIGVPLETHAGETRVSATPETVKKLIGQGHQVIVQSGAGVNASQPDSAYEAAGATIGSAAEAFGADLVLKV
VAPSAAELAQMKSGAVLVGMLNPFDNENIARMAERGITAFALEAAPRTSRAQSLDVLSSQANIAGYKAVMLA
ANHYPRFMPMLMTAAGTVKAARVLILGAGVAGLQAIATAKRLGAVIEASDVRPAVKEQIESLGAKFVDVPYE
TDEERECAEGVGGYARPMPASWMERQAKAVHERAKQSDIVITTALIPGRKAPTLLHEATVAEMKPGSVVIDL
AAAQGGNCPLTEADQVVVRHGVTIVGHSNLAALVPADASALYARNILDFLKLTLNAEGFSVNLEDDIVAACL
MCRDGQAVRKNG*
AnnotationID:2427GeneID:2419
AnnotatorUID: danlee
Modification Date/Time: 2006-03-16 16:04:42
Gene Name:
Confidence Code:3
GeneProduct:putative NAD(P) transhydrogenase, subunit alpha part 1
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:pntA N-terminus
Functional Category Confidence Code:5
COGs:COG3288,pfam05222
Secondary Functional Category(ies):amino acid transport and metabolism
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD16437.1| (AL646071) PROBABLE NAD(P) TRANSHYDROGENASE (ALPHA SUBUNIT PART 1)
           OXIDOREDUCTASE PROTEIN [Ralstonia solanacearum]
          Length = 379

 Score =  568 bits (1463), Expect = e-161
 Identities = 291/370 (78%), Positives = 328/370 (88%), Gaps = 1/370 (0%)

>emb|CAE43169.1| (BX640419) NAD(P) transhydrogenase, subunit alpha part 1
           [Bordetella pertussis]
          Length = 372

 Score =  510 bits (1313), Expect = e-144
 Identities = 261/372 (70%), Positives = 307/372 (82%), Gaps = 1/372 (0%)

>emb|CAE32443.1| (BX640442) NAD(P) transhydrogenase, subunit alpha part 1
           [Bordetella bronchiseptica]
          Length = 372

 Score =  509 bits (1312), Expect = e-144
 Identities = 261/372 (70%), Positives = 306/372 (82%), Gaps = 1/372 (0%)








 
Structural Features:
COG3288, PntA, NAD/NADP transhydrogenase alpha subunit 
[Energy production and conversion]
CD-Length = 356 residues, 100.0% aligned
Score =  418 bits (1076), Expect = 5e-118
Identities = 200/366 (54%), Positives = 255/366 (69%), Gaps = 11/366 (3%)

pfam05222, AlaDh_PNT_N, Alanine dehydrogenase/PNT, N-terminal domain.
CD-Length = 136 residues, 100.0% aligned
Score =  129 bits (327), Expect = 4e-31
Identities = 64/136 (47%), Positives = 83/136 (61%), Gaps = 3/136 (2%)







   
Genomic Context:






   
Comment:
Matches 3' end of PA0195 (Identities = 371/372 (99%), Positives = 372/372 (99%) for first 372 
amino acids of PA0195).


















    ReferenceID:14583




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21987




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33562




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2419_cdd.html


    		




 
URL:

















    ReferenceID:35481




  
Author/Investigator(s):
        Williams R, Cotton NP, Thomas CM, Jackson JB.





  
Title:
        Cloning and sequencing of the genes for the proton-translocating nicotinamide nucleotide transhydrogenase from Rhodospirillum rubrum and the implications for the domain structure of the enzyme.




  
PubMed:
        8075801


MedLine:
        




    
Source:
Microbiology. 1994 Jul;140 ( Pt 7):1595-604.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8075801









           
Homologs By Global Alignment 

           

       



     
Gene ID:2419
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
122044
                           gb|AAN62244.1|AF440524_31
                           (AF440524) putative proton-translocating NAD(P) transhydrogenase [Pseudomonas aeruginosa]
                           404
                           81.18
                           86.63
                           32
                           1638.0
     

     
     
122045
                           emb|CAD16437.1|
                           (AL646071) PROBABLE NAD(P) TRANSHYDROGENASE (ALPHA SUBUNIT PART 1) OXIDOREDUCTASE PROTEIN [Ralstonia solanacearum]
                           379
                           76.51
                           86.54
                           7
                           1458.0
     

     
     
122046
                           emb|CAE43169.1|
                           (BX640419) NAD(P) transhydrogenase, subunit alpha part 1 [Bordetella pertussis] [Bordetella pertussis Tohama I]
                           373
                           69.70
                           82.30
                           2
                           1311.0
     

     
     
122047
                           emb|CAE32443.1|
                           (BX640442) NAD(P) transhydrogenase, subunit alpha part 1 [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           373
                           69.70
                           82.03
                           2
                           1310.0
     

     
     
122048
                           emb|CAE37794.1|
                           (BX640430) NAD(P) transhydrogenase, subunit alpha part 1 [Bordetella parapertussis]
                           373
                           69.70
                           82.03
                           2
                           1310.0
     

     






      

          
GC ORFID: 45421How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: psyr_15may02_Scaffold2_revised_gene2883Glimmer Score: 

          
Start: 6022178Stop: 6023296

          
Length: 1119


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: psyr_15may02_Scaffold2_revised_gene2883 related to: gi|15600764|ref|NP_248885.1| (NC_002516) NAD/NADP transhydrogenase, NAD(H)-binding dI subunit [Pseudomonas aeruginosa]Homolog Bit Score 607.0

          
Other Homologs:  t_PA0195 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGCAGATCGGTGTCCCCCTCGAGACCCATGCCGGTGAGACGCGGGTTTCCGCCACCCCGGAAACCGTCAAG 
AAGCTGATCGGCCAAGGCCACCAGGTGATCGTCCAGAGCGGCGCCGGCGTCAACGCCAGCCAGCCGGACAGT 
GCCTATGAAGCCGCCGGCGCGACGATCGGCAGCGCCGCCGAGGCCTTCGGCGCCGACCTGGTGCTGAAGGTG 
GTCGCGCCCAGCGCCGCCGAGCTGGCCCAGATGAAAAGCGGTGCCGTGCTGGTCGGCATGCTCAACCCCTTC 
GACAACGAGAACATCGCCCGCATGGCCGAGCGCGGCATCACCGCCTTCGCCCTCGAGGCCGCGCCGCGTACC 
TCGCGGGCGCAGAGCCTGGATGTGCTCTCCTCGCAGGCCAACATCGCCGGCTACAAGGCGGTGATGCTCGCC 
GCCAACCACTACCCGCGCTTCATGCCGATGCTGATGACCGCCGCCGGCACCGTGAAGGCCGCCCGCGTGCTG 
ATCCTCGGCGCCGGCGTCGCCGGCCTGCAGGCGATCGCCACGGCCAAGCGCCTGGGCGCGGTGATCGAAGCT 
TCCGACGTGCGTCCGGCGGTGAAGGAGCAGATCGAGTCGCTCGGCGCCAAGTTCGTCGACGTGCCCTACGAG 
ACCGACGAAGAGCGCGAGTGCGCCGAAGGCGTCGGCGGCTATGCGCGGCCGATGCCGGCCTCGTGGATGGAG 
CGCCAGGCCAAGGCCGTGCACGAGCGCGCCAAGCAGTCGGACATCGTCATCACTACCGCGCTGATCCCCGGC 
CGCAAGGCGCCGACGCTGCTGCACGAGGCGACCGTGGCGGAGATGAAGCCGGGCTCGGTGGTCATCGACCTG 
GCCGCCGCCCAGGGCGGCAACTGCCCGCTGACCGAGGCCGACCAGGTCGTGGTCCGGCACGGCGTGACCATC 
GTCGGCCACAGCAACCTGGCCGCGCTGGTACCGGCGGACGCCTCGGCGCTATACGCGCGCAACATCCTCGAC 
TTCCTCAAGCTCACCCTGAACGCCGAAGGCTTCAGCGTGAACCTCGAGGACGACATCGTCGCCGCCTGCCTG 
ATGTGCCGCGACGGCCAGGCGGTGCGCAAGAACGGCTGA