ViewGene
Locus Name:
GeneID:
Location:

GeneID:2412
TrivialName: PA14_18260
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18260
PAO1 Ortholog Locus:PA3561
Sequence Length:945
Protein Length:314
Start: 1566589
Stop: 1567533
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06949.1|AE004777_2 (AE004777) 1-phosphofructokinase [Pseudomonas aeruginosa PAO1]
Identities = 312/314 (99%), Positives = 313/314 (99%)
Sequence:
ATGGCGCGGATCCTGACGATTACCCTGAATCCGGCGCTGGACCTCACCGTGCGCCTGCGCCAGCTGGAACTG
GGGGCGGTTAACCGCAGCGAGGCGGTGCTGACCCAGGCCGCCGGCAAGGGCCTGAACGTCGCCCAGGTGCTT
GCCGACCTCGGCCATCGGTTGACGGTCAGCGGCTTCCTCGGCGAAGACAACCAGCCGCCGTTCACCGCCATG
TTCCAGCGTCGTGGCTTCGCCGATGCGTTCGTCCGGGTGCCGGGCGAAACCCGCAGCAACATCAAGCTGGCC
GAGCGCGGCGGGCGCGTCAGCGACCTCAACGGGCCGGGCCCGGAGGCCGACGAGCGAGCCCAGGCGGCGTTG
CTCGAACGCCTCGACCGGCTTGCCGGGGAGCACGAGCTGGCGGTGGTCGCCGGCAGCCTGCCGCGCGGGGTC
GAGCCGGAATGGCTGGGCGAACTGCTGCGGCGCTTGCGGCGCCTCGGCCTGAAGGTCGCCTTCGACAGCAGC
GGCGCGGCCCTGCGCGAAGGTGTGAAGGCTGCGCCGTGGATGATCAAGCCGAACGTCGAGGAACTGGCCGAC
CTCTGCTCGGCGCCGATGGATGACCTGGCCGCACAGCGCCAGGCGGCCGAACGCCTGCGCCATGACGGGGTA
GGCCAGGTGGTGATTTCCCAGGGCGCCGCGGGGGTCAACTGGTTCGCCGCCGAGGGTGCCTGGCAGGCGCGG
CCGCCGGCGGTGGACGTGGCCAGCACGGTGGGCGCCGGCGATTCCCTGCTGGCCGGCATGCTGCACGGCCTG
GCCAGCGGCTGGCCGGCCGAGCGGGTGCTGCGTCAGGCCACCGCCATCGCCAGCCTGGCGGTGACCCAGTTC
GAGTTCGGCATCGGCGATCCGCAGCGCCTGGCGCGGATCGAAGCGGGCGTCGTGGTGCAGCCATTGGTCGAG
GAGGCCTGA
Translation:
MARILTITLNPALDLTVRLRQLELGAVNRSEAVLTQAAGKGLNVAQVLADLGHRLTVSGFLGEDNQPPFTAM
FQRRGFADAFVRVPGETRSNIKLAERGGRVSDLNGPGPEADERAQAALLERLDRLAGEHELAVVAGSLPRGV
EPEWLGELLRRLRRLGLKVAFDSSGAALREGVKAAPWMIKPNVEELADLCSAPMDDLAAQRQAAERLRHDGV
GQVVISQGAAGVNWFAAEGAWQARPPAVDVASTVGAGDSLLAGMLHGLASGWPAERVLRQATAIASLAVTQF
EFGIGDPQRLARIEAGVVVQPLVEEA*
AnnotationID:2420GeneID:2412
AnnotatorUID: diggins
Modification Date/Time: 2005-07-10 11:11:40
Gene Name:fruK
Confidence Code:2
GeneProduct:1-phosphofructokinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(27) Transport of small molecules
Alternate Gene Product Name:fructose-1-phosphate kinase
Functional Category Confidence Code:5
COGs:COG1105,pfam00294
Secondary Functional Category(ies):central intermediary metabolism
EC Number:2.7.1.56
Status:ACTIVE
Pathway:
Fructose and mannose metabolism
Homology:
>gb|AAO54489.1| (AE016859) 1-phosphofructokinase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 313

 Score =  389 bits (999), Expect = e-107
 Identities = 200/313 (63%), Positives = 241/313 (76%)

>gb|AAO07171.1|AE016808_191 (AE016808) Fructose-1-phosphate kinase [Vibrio vulnificus CMCP6]
          Length = 325

 Score =  253 bits (646), Expect = 1e-66
 Identities = 141/309 (45%), Positives = 195/309 (63%), Gaps = 2/309 (0%)
Structural Features:
COG1105, FruK, Fructose-1-phosphate kinase and related fructose-6-phosphate 
kinase (PfkB) 
[Carbohydrate transport and metabolism]
CD-Length = 310 residues, 100.0% aligned
Score =  349 bits (896), Expect = 7e-97
Identities = 143/310 (46%), Positives = 183/310 (59%), Gaps = 1/310 (0%)

pfam00294, PfkB, pfkB family carbohydrate kinase
CD-Length = 298 residues,  92.6% aligned
Score =  118 bits (296), Expect = 2e-27
Identities = 81/283 (28%), Positives = 113/283 (39%), Gaps = 17/283 (6%)
Genomic Context:






   
Comment:
other possible gene name: fpk


















    ReferenceID:4827




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21973




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31983




  
Author/Investigator(s):
        





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2412_cdd.html


    		




 
URL:

















    ReferenceID:34150




  
Author/Investigator(s):
        Wu LF, Reizer A, Reizer J, Cai B, Tomich JM, Saier MH Jr.





  
Title:
        Nucleotide sequence of the Rhodobacter capsulatus fruK gene, which encodes fructose-1-phosphate kinase: evidence for a kinase superfamily including both phosphofructokinases of Escherichia coli.




  
PubMed:
        1850730


MedLine:
        




    
Source:
J Bacteriol. 1991 May;173(10):3117-27.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1850730

















    ReferenceID:34151




  
Author/Investigator(s):
        Orchard LM, Kornberg HL.





  
Title:
        Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus.




  
PubMed:
        1981619


MedLine:
        




    
Source:
Proc R Soc Lond B Biol Sci. 1990 Nov 22;242(1304):87-90.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1981619









           
Homologs By Global Alignment 

           

       



     
Gene ID:2412
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4063
                           PA3561_tr
                           translation of PA3561
                           314
                           99.36
                           99.68
                           0
                           1566.0
     

     
     
121902
                           gb|AAG06949.1|AE004777_2
                           (AE004777) 1-phosphofructokinase [Pseudomonas aeruginosa PAO1]
                           314
                           99.36
                           99.68
                           0
                           1566.0
     

     
     
121903
                           gb|AAO54489.1|
                           (AE016859) 1-phosphofructokinase [Pseudomonas syringae pv. tomato str. DC3000]
                           314
                           63.69
                           76.75
                           1
                           999.0
     

     
     
121904
                           gb|AAN66419.1|AE016269_6
                           (AE016777) 1-phosphofructokinase [Pseudomonas putida KT2440]
                           318
                           58.17
                           71.69
                           7
                           905.0
     

     
     
121913
                           emb|CAA37896.1|
                           (X53948) 1-phosphofructokinase [Escherichia coli]
                           319
                           45.14
                           58.30
                           12
                           616.0
     

     






      

          
GC ORFID: 45414How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3561Glimmer Score: 

          
Start: 3387347Stop: 3386403

          
Length: 945


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3561 translation of PA3561Homolog Bit Score 607.0

          
Other Homologs:  psyr_15may02_Scaffold15_revised_gene2495 (ORF: BLASTX 392.0), t_PA3561 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCGCGGATCCTGACGATTACCCTGAATCCGGCGCTGGACCTCACCGTGCGCCTGCGCCAGCTGGAACTG 
GGGGCGGTTAACCGCAGCGAGGCGGTGCTGACCCAGGCCGCCGGCAAGGGCCTGAACGTCGCCCAGGTGCTT 
GCCGACCTCGGCCATCGGTTGACGGTCAGCGGCTTCCTCGGCGAAGACAACCAGCCGCCGTTCACCGCCATG 
TTCCAGCGTCGTGGCTTCGCCGATGCGTTCGTCCGGGTGCCGGGCGAAACCCGCAGCAACATCAAGCTGGCC 
GAGCGCGGCGGGCGCGTCAGCGACCTCAACGGGCCGGGCCCGGAGGCCGACGAGCGAGCCCAGGCGGCGTTG 
CTCGAACGCCTCGACCGGCTTGCCGGGGAGCACGAGCTGGCGGTGGTCGCCGGCAGCCTGCCGCGCGGGGTC 
GAGCCGGAATGGCTGGGCGAACTGCTGCGGCGCTTGCGGCGCCTCGGCCTGAAGGTCGCCTTCGACAGCAGC 
GGCGCGGCCCTGCGCGAAGGTGTGAAGGCTGCGCCGTGGATGATCAAGCCGAACGTCGAGGAACTGGCCGAC 
CTCTGCTCGGCGCCGATGGATGACCTGGCCGCACAGCGCCAGGCGGCCGAACGCCTGCGCCATGACGGGGTA 
GGCCAGGTGGTGATTTCCCAGGGCGCCGCGGGGGTCAACTGGTTCGCCGCCGAGGGTGCCTGGCAGGCGCGG 
CCGCCGGCGGTGGACGTGGCCAGCACGGTGGGCGCCGGCGATTCCCTGCTGGCCGGCATGCTGCACGGCCTG 
GCCAGCGGCTGGCCGGCCGAGCGGGTGCTGCGTCAGGCCACCGCCATCGCCAGCCTGGCGGTGACCCAGTTC 
GAGTTCGGCATCGGCGATCCGCAGCGCCTGGCGCGGATCGAAGCGGGCGTCGTGGTGCAGCCATTGGTCGAG 
GAGGCCTGA