ViewGene
Locus Name:
GeneID:
Location:

GeneID:2401
TrivialName: PA14_34440
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_34440
PAO1 Ortholog Locus:PA2337
Sequence Length:906
Protein Length:301
Start: 3063556
Stop: 3062651
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05725.1|AE004660_1 (AE004660) transcriptional regulator MtlR [Pseudomonas aeruginosa PAO1]
Identities = 301/301 (100%), Positives = 301/301 (100%)
Sequence:
ATGCCCAGAGCCAGCAAAGTCACCGATCCGTCCTACGAGCTGATGGACGACCACGAAGGCCATTCGCTGATC
TACCGCCAGCACGGCTTCCCCAGCCCGCTGGTGCGCTGGCACTTCCACAAGGAATACGAACTGCACCTGATC
GTCGCCAGCTCCGGCAAGGTATTCATCGGCGACTACATCGGCAACTTCGCCCCCGACACCCTGTTCCTCACC
GGCCCCAACCTGCCGCACAACTGGATCAGCCAGGTCGAGCCCGACGAGGTGGTGGCCCGGCGCGACATGCTG
GTGAACTTCACCGACGAGGTGCTGGAGGACGGCAGCGGCGTATTCTCCGAGCTGAACCGCCTGGCCCCGCTG
CTGGCGCGGGCGCGCTACGGCATCGAGTTCCGCGACCCGGCGCTGATCGGCGAAAGCCGGCGACTGTTGCAA
CGCATCGCCGACAGCGGCGGCATGACCCGCCTGGGATACTTTTTTATCCTCATGGAACAGCTCGCCGAATGC
GAGGACTACCAGTTGCTCTCCACCGTCACGTCCTCGCAACTGGCCGACGAGCACAACGTCGAACGGATCAAC
CGGGCGGTGGACTACATCTTCCAGCACTACGCCCAGGACCTCACCCAGGAGCAGGTCGCCGCCCACCTGGGC
ATGACCCCCACCTACTTCTCGCGCTTCTTCAAGCAGGCCGCCGGGCGCGGCTTCGTCGAGTTCGTCAACCGC
CTGCGCATCAGCAAGTCCTGCGAGCTGCTGGCCCGCAGCGAGCTGCCGGTGACCGAGGTGTGCTTCGAGTCG
GGCTTCAGCAACCTGTCCAATTTCAACCGGCGCTTCCAGCAGCTCAAGGGCATGACGCCCACCGGCTACCGG
CGCCTGGTGACCCAGCGGCTGACCGAGCAGAACCTGCTCTGA
Translation:
MPRASKVTDPSYELMDDHEGHSLIYRQHGFPSPLVRWHFHKEYELHLIVASSGKVFIGDYIGNFAPDTLFLT
GPNLPHNWISQVEPDEVVARRDMLVNFTDEVLEDGSGVFSELNRLAPLLARARYGIEFRDPALIGESRRLLQ
RIADSGGMTRLGYFFILMEQLAECEDYQLLSTVTSSQLADEHNVERINRAVDYIFQHYAQDLTQEQVAAHLG
MTPTYFSRFFKQAAGRGFVEFVNRLRISKSCELLARSELPVTEVCFESGFSNLSNFNRRFQQLKGMTPTGYR
RLVTQRLTEQNLL*
AnnotationID:2409GeneID:2401
AnnotatorUID: diggins
Modification Date/Time: 2005-03-04 15:03:54
Gene Name:mtlR
Confidence Code:2
GeneProduct: transcriptional regulator MtlR
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(25) Transcriptional regulators
Alternate Gene Product Name:activator protein
Functional Category Confidence Code:5
COGs:COG2207,smart00342
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC34292.1| (AF047527) activator protein [Pseudomonas fluorescens]
          Length = 301

 Score =  463 bits (1191), Expect = e-130
 Identities = 223/300 (74%), Positives = 255/300 (85%)

>gb|AAO56209.1| (AE016865) transcriptional activator MltR [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 301

 Score =  449 bits (1156), Expect = e-126
 Identities = 219/300 (73%), Positives = 252/300 (84%)








 
Structural Features:
COG2207, AraC, AraC-type DNA-binding domain-containing proteins 
[Transcription]
CD-Length = 127 residues,  95.3% aligned
Score = 96.1 bits (238), Expect = 5e-21
Identities = 40/121 (33%), Positives = 73/121 (60%)

smart00342, HTH_ARAC, helix_turn_helix, arabinose operon control protein
CD-Length = 84 residues, 100.0% aligned
Score = 89.9 bits (223), Expect = 3e-19
Identities = 31/84 (36%), Positives = 51/84 (60%)







   
Genomic Context:






   
Comment:


















    ReferenceID:4805




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21951




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32463




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2401_cdd.html


    		




 
URL:

















    ReferenceID:34467




  
Author/Investigator(s):
        Figge RM, Ramseier TM, Saier MH Jr.





  
Title:
        The mannitol repressor (MtlR) of Escherichia coli.




  
PubMed:
        8300537


MedLine:
        




    
Source:
J Bacteriol. 1994 Feb;176(3):840-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8300537

















    ReferenceID:34468




  
Author/Investigator(s):
        Henstra SA, Tolner B, ten Hoeve Duurkens RH, Konings WN, Robillard GT.





  
Title:
        Cloning, expression, and isolation of the mannitol transport protein from the thermophilic bacterium Bacillus stearothermophilus.




  
PubMed:
        8824601


MedLine:
        




    
Source:
J Bacteriol. 1996 Oct;178(19):5586-91.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8824601

















    ReferenceID:34469




  
Author/Investigator(s):
        Brunker P, Hils M, Altenbuchner J, Mattes R.





  
Title:
        The mannitol utilization genes of Pseudomonas fluorescens are regulated by an activator: cloning, nucleotide sequence and expression of the mtlR gene.




  
PubMed:
        9666063


MedLine:
        




    
Source:
Gene. 1998 Jul 17;215(1):19-27.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9666063

















    ReferenceID:34470




  
Author/Investigator(s):
        Otte S, Lengeler JW.





  
Title:
        The mtl genes and the mannitol-1-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026.




  
PubMed:
        11164312


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2001 Jan 15;194(2):221-7.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11164312









           
Homologs By Global Alignment 

           

       



     
Gene ID:2401
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4052
                           PA2337_tr
                           translation of PA2337
                           301
                           99.99
                           99.99
                           0
                           1568.0
     

     
     
121675
                           gb|AAG05725.1|AE004660_1
                           (AE004660) transcriptional regulator MtlR [Pseudomonas aeruginosa PAO1]
                           301
                           99.99
                           99.99
                           0
                           1568.0
     

     
     
121676
                           gb|AAC34292.1|
                           (AF047527) activator protein [Pseudomonas fluorescens]
                           301
                           74.08
                           84.71
                           0
                           1190.0
     

     
     
121677
                           gb|AAO56209.1|
                           (AE016865) transcriptional activator MltR [Pseudomonas syringae pv. tomato str. DC3000]
                           301
                           72.75
                           83.72
                           0
                           1155.0
     

     






      

          
GC ORFID: 45403How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2337Glimmer Score: 

          
Start: 1890360Stop: 1891265

          
Length: 906


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2337 translation of PA2337Homolog Bit Score 608.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3064 (ORF: BLASTX 448.0), t_PA2337 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCCAGAGCCAGCAAAGTCACCGATCCGTCCTACGAGCTGATGGACGACCACGAAGGCCATTCGCTGATC 
TACCGCCAGCACGGCTTCCCCAGCCCGCTGGTGCGCTGGCACTTCCACAAGGAATACGAACTGCACCTGATC 
GTCGCCAGCTCCGGCAAGGTATTCATCGGCGACTACATCGGCAACTTCGCCCCCGACACCCTGTTCCTCACC 
GGCCCCAACCTGCCGCACAACTGGATCAGCCAGGTCGAGCCCGACGAGGTGGTGGCCCGGCGCGACATGCTG 
GTGAACTTCACCGACGAGGTGCTGGAGGACGGCAGCGGCGTATTCTCCGAGCTGAACCGCCTGGCCCCGCTG 
CTGGCGCGGGCGCGCTACGGCATCGAGTTCCGCGACCCGGCGCTGATCGGCGAAAGCCGGCGACTGTTGCAA 
CGCATCGCCGACAGCGGCGGCATGACCCGCCTGGGATACTTTTTTATCCTCATGGAACAGCTCGCCGAATGC 
GAGGACTACCAGTTGCTCTCCACCGTCACGTCCTCGCAACTGGCCGACGAGCACAACGTCGAACGGATCAAC 
CGGGCGGTGGACTACATCTTCCAGCACTACGCCCAGGACCTCACCCAGGAGCAGGTCGCCGCCCACCTGGGC 
ATGACCCCCACCTACTTCTCGCGCTTCTTCAAGCAGGCCGCCGGGCGCGGCTTCGTCGAGTTCGTCAACCGC 
CTGCGCATCAGCAAGTCCTGCGAGCTGCTGGCCCGCAGCGAGCTGCCGGTGACCGAGGTGTGCTTCGAGTCG 
GGCTTCAGCAACCTGTCCAATTTCAACCGGCGCTTCCAGCAGCTCAAGGGCATGACGCCCACCGGCTACCGG 
CGCCTGGTGACCCAGCGGCTGACCGAGCAGAACCTGCTCTGA