ViewGene
Locus Name:
GeneID:
Location:

GeneID:2398
TrivialName: PA14_00190
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_00190
PAO1 Ortholog Locus:PA0018
Sequence Length:945
Protein Length:314
Start: 21034
Stop: 20090
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03408.1|AE004441_9 (AE004441) methionyl-tRNA formyltransferase [Pseudomonas aeruginosa PAO1]
Identities = 310/314 (98%), Positives = 312/314 (99%)
Sequence:
ATGAGCCAAGCATTGCGCATCGTCTTCGCCGGAACCCCGGAATTCGCCGCCGAGCATCTCAAGGCCCTGCTC
GACACCCCACATCGGATCGTCGCCGTCTACACCCAGCCCGACCGGCCGGCCGGCCGCGGGCAGAAACTGATG
CCCAGCGCGGTGAAGAGCCTGGCCCTGGAGCATGGCCTACCGGTCATGCAGCCGCAGAGCCTGCGCAATGCC
GAGGCCCAGGCGGAGCTGGCGGCGCTGCGCGCGGACCTGATGGTGGTGGTCGCCTATGGCCTGATCCTGCCC
CAGGCGGTACTCGATATCCCGCGCCTGGGCTGCATCAACAGCCACGCCTCGCTGCTGCCGCGCTGGCGCGGC
GCCGCGCCGATCCAGCGCGCGGTGGAAGCCGGCGACGCGGAGAGCGGCGTCACCGTGATGCAGATGGAAGCA
GGGCTCGACACCGGCCCGATGCTGCTCAAGGTGAGCACGCCGATTTCCGCCGCGGACACCGGCGGCAGCCTG
CACGACCGGCTCGCCGCGCTCGGCCCGAAAGCGGTGGTCGAAGCCATCGCCGGCCTGGCCGCCGGCACCCTG
CATGGCGAGGACCAGGACGACGCCCTGGCCACCTACGCGCACAAGCTGAACAAGGACGAGGCACGCCTCGAC
TGGAGCCGTCCGGCCGTCGAACTGGAGCGCCAGGTCCGCGCCTTCACCCCCTGGCCGGTCTGCCACACCAGC
CTCGCCGATGCGCCGCTGAAAGTCCTCGGTGCCAGCCTGGGGCAGGGCAGCGGGGCGCCCGGAACCATCCTC
GAGGCCAGCCGCGACGGCCTGCTGGTCGCCTGCGGCGAAGGCGCCCTGCGCCTGACCCGCCTGCAAGTGCCG
GGCGGCAAGCCACTGGCCTTCGCCGACCTCTACAACAGCCGTCGCGAGCAATTCGCCACCGGCCAGGTGCTC
GGCCAATGA
Translation:
MSQALRIVFAGTPEFAAEHLKALLDTPHRIVAVYTQPDRPAGRGQKLMPSAVKSLALEHGLPVMQPQSLRNA
EAQAELAALRADLMVVVAYGLILPQAVLDIPRLGCINSHASLLPRWRGAAPIQRAVEAGDAESGVTVMQMEA
GLDTGPMLLKVSTPISAADTGGSLHDRLAALGPKAVVEAIAGLAAGTLHGEDQDDALATYAHKLNKDEARLD
WSRPAVELERQVRAFTPWPVCHTSLADAPLKVLGASLGQGSGAPGTILEASRDGLLVACGEGALRLTRLQVP
GGKPLAFADLYNSRREQFATGQVLGQ*
AnnotationID:2406GeneID:2398
AnnotatorUID: diggins
Modification Date/Time: 2005-03-22 11:11:04
Gene Name:fmt
Confidence Code:2
GeneProduct:methionyl-tRNA formyltransferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(26) Translation, post-translational modification, degradation
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0223
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:2.1.2.9
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO53732.1| (AE016856) methionyl-tRNA formyltransferase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 314

 Score =  508 bits (1307), Expect = e-143
 Identities = 252/314 (80%), Positives = 280/314 (89%)

>gb|AAN65701.1|AE016196_12 (AE016774) methionyl-tRNA formyltransferase [Pseudomonas putida
           KT2440]
          Length = 310

 Score =  504 bits (1297), Expect = e-142
 Identities = 250/310 (80%), Positives = 280/310 (90%)

>emb|CAA45207.1| (X63666) methionyl-tRNA formyltransferase [Escherichia coli]
          Length = 315

 Score =  365 bits (938), Expect = e-100
 Identities = 189/314 (60%), Positives = 234/314 (74%), Gaps = 2/314 (0%)








 
Structural Features:
COG0223, Fmt, Methionyl-tRNA formyltransferase 
[Translation, ribosomal structure and biogenesis]
CD-Length = 307 residues,  98.0% aligned
Score =  398 bits (1024), Expect = 5e-112
Identities = 167/301 (55%), Positives = 211/301 (70%), Gaps = 2/301 (0%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4799




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21945




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33382




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2398_cdd.html


    		




 
URL:

















    ReferenceID:35524




  
Author/Investigator(s):
        Komatsuzawa H, Sugai M, Ohta K, Fujiwara T, Nakashima S, Suzuki J, Lee CY, Suginaka H.





  
Title:
        Cloning and characterization of the fmt gene which affects the methicillin resistance level and autolysis in the presence of triton X-100 in methicillin-resistant Staphylococcus aureus.




  
PubMed:
        9371333


MedLine:
        




    
Source:
Antimicrob Agents Chemother. 1997 Nov;41(11):2355-61.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9371333









           
Homologs By Global Alignment 

           

       



     
Gene ID:2398
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4049
                           PA0018_tr
                           translation of PA0018
                           314
                           98.72
                           99.36
                           0
                           1571.0
     

     
     
121596
                           gb|AAG03408.1|AE004441_9
                           (AE004441) methionyl-tRNA formyltransferase [Pseudomonas aeruginosa PAO1]
                           314
                           98.72
                           99.36
                           0
                           1571.0
     

     
     
121597
                           gb|AAC26787.1|
                           (AF073952) methionyl-tRNA formyltransferase [Pseudomonas aeruginosa]
                           314
                           98.72
                           99.36
                           0
                           1571.0
     

     
     
121598
                           gb|AAO53732.1|
                           (AE016856) methionyl-tRNA formyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           314
                           80.25
                           89.17
                           0
                           1307.0
     

     
     
121599
                           gb|AAN65701.1|AE016196_12
                           (AE016774) methionyl-tRNA formyltransferase [Pseudomonas putida KT2440]
                           314
                           79.61
                           89.17
                           4
                           1297.0
     

     






      

          
GC ORFID: 45400How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0018Glimmer Score: 

          
Start: 5822371Stop: 5821427

          
Length: 945


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0018 translation of PA0018Homolog Bit Score 609.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene4099 (ORF: BLASTX 505.0), t_PA0018 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCCAAGCATTGCGCATCGTCTTCGCCGGAACCCCGGAATTCGCCGCCGAGCATCTCAAGGCCCTGCTC 
GACACCCCACATCGGATCGTCGCCGTCTACACCCAGCCCGACCGGCCGGCCGGCCGCGGGCAGAAACTGATG 
CCCAGCGCGGTGAAGAGCCTGGCCCTGGAGCATGGCCTACCGGTCATGCAGCCGCAGAGCCTGCGCAATGCC 
GAGGCCCAGGCGGAGCTGGCGGCGCTGCGCGCGGACCTGATGGTGGTGGTCGCCTATGGCCTGATCCTGCCC 
CAGGCGGTACTCGATATCCCGCGCCTGGGCTGCATCAACAGCCACGCCTCGCTGCTGCCGCGCTGGCGCGGC 
GCCGCGCCGATCCAGCGCGCGGTGGAAGCCGGCGACGCGGAGAGCGGCGTCACCGTGATGCAGATGGAAGCA 
GGGCTCGACACCGGCCCGATGCTGCTCAAGGTGAGCACGCCGATTTCCGCCGCGGACACCGGCGGCAGCCTG 
CACGACCGGCTCGCCGCGCTCGGCCCGAAAGCGGTGGTCGAAGCCATCGCCGGCCTGGCCGCCGGCACCCTG 
CATGGCGAGGACCAGGACGACGCCCTGGCCACCTACGCGCACAAGCTGAACAAGGACGAGGCACGCCTCGAC 
TGGAGCCGTCCGGCCGTCGAACTGGAGCGCCAGGTCCGCGCCTTCACCCCCTGGCCGGTCTGCCACACCAGC 
CTCGCCGATGCGCCGCTGAAAGTCCTCGGTGCCAGCCTGGGGCAGGGCAGCGGGGCGCCCGGAACCATCCTC 
GAGGCCAGCCGCGACGGCCTGCTGGTCGCCTGCGGCGAAGGCGCCCTGCGCCTGACCCGCCTGCAAGTGCCG 
GGCGGCAAGCCACTGGCCTTCGCCGACCTCTACAACAGCCGTCGCGAGCAATTCGCCACCGGCCAGGTGCTC 
GGCCAATGA