ViewGene
Locus Name:
GeneID:
Location:

GeneID:2397
TrivialName: PA14_57450
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57450
PAO1 Ortholog Locus:PA4420
Sequence Length:942
Protein Length:313
Start: 5119129
Stop: 5118188
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07808.1|AE004856_19 (AE004856) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 311/313 (99%), Positives = 311/313 (99%)
Sequence:
ATGAATGCCACCTACCGCCATATCACCGTTCTGCTCGAGGAGGCCGTTTCCGCACTGGCCCCGCGGGAGGAC
GGCTGCTATCTGGACGGTACCTTCGGAAGAGGGGGCCATAGTCGAGCCTTGCTCGAGAAACTGGGCACCGGG
GGACGCCTGCTCGGATTCGACAAAGATCCCCAGGCCATCCAGACAGGAAAAGCATTGGCGGCCGAAGACGGC
CGCTTCGTCATTGTGCAGCGCTCGTTCGCCGAACTGGGCGATGAGGTGCGCGCACGCGGTCTCGAAGGTCGG
GTCGATGGCGTCCTGCTCGACCTTGGCGTGTCGTCGCCGCAACTCGACGATCCCGAGCGCGGCTTCAGCTTC
CTCAACGATGGTCCCCTGGACATGCGCATGAATCCCGGCCAGGGCATCAGCGCAGCGGAGTTCATCGCCAAC
GCCACCGAAGAGGAAATCGCCCGGGTCTTCAAGGTATATGGCGAAGAGCGCTTCGCCAAGCGGATGGCCCGC
GCCATTGTGCAGCGCCGTCAGGAGCGGCCGTTCGAACGGACCGCTGATCTCGCCGAAGTGATCACGGTTGCC
AACCCCGCCTGGGAGAAGGGCAAGAATCCGGCGACCCGCGCATTCCAGGGCCTGCGCATCCATGTGAACAAT
GAATTGGGCGATCTCGAGCGCGGCCTTGACGCCGCGCTCGAATCGCTGGCCGTCGGCGGCCGCCTGGTGGTG
ATCAGTTTCCATTCCCTGGAAGACCGCATCGTCAAGCTGTTCATGCGCAAGCACGCCAAGGGCGAGGCGGAC
AACCTGCCGCGCGACCTGCCGATCCGTTCCAAGGTCTTCGAGCCGCGCCTGAAGCTGCTGGGCAAGCCGCAG
TACGCTTCGGAAGAAGAACTCAAGGCCAACCCGCGCTCGCGCAGCGCGGTCATGCGCGTGGCGGAGAAGCTG
AGATGA
Translation:
MNATYRHITVLLEEAVSALAPREDGCYLDGTFGRGGHSRALLEKLGTGGRLLGFDKDPQAIQTGKALAAEDG
RFVIVQRSFAELGDEVRARGLEGRVDGVLLDLGVSSPQLDDPERGFSFLNDGPLDMRMNPGQGISAAEFIAN
ATEEEIARVFKVYGEERFAKRMARAIVQRRQERPFERTADLAEVITVANPAWEKGKNPATRAFQGLRIHVNN
ELGDLERGLDAALESLAVGGRLVVISFHSLEDRIVKLFMRKHAKGEADNLPRDLPIRSKVFEPRLKLLGKPQ
YASEEELKANPRSRSAVMRVAEKLR*
AnnotationID:2405GeneID:2397
AnnotatorUID: diggins
Modification Date/Time: 2005-05-12 08:08:01
Gene Name:
Confidence Code:3
GeneProduct:putative methyltransferase
Cell Localization:()
Synonyms:mraW
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0275,pfam01795
Secondary Functional Category(ies):membrane proteins
EC Number:2.1.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57865.1| (AE016871) S-adenosyl-methyltransferase MraW [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 313

 Score =  503 bits (1294), Expect = e-142
 Identities = 253/313 (80%), Positives = 280/313 (89%)

>gb|AAG54386.1|AE005185_3 (AE005185) putative apolipoprotein [Escherichia coli O157:H7
           EDL933]
          Length = 313

 Score =  329 bits (843), Expect = 1e-89
 Identities = 176/315 (55%), Positives = 228/315 (72%), Gaps = 9/315 (2%)







 
Structural Features:
COG0275, Predicted S-adenosylmethionine-dependent methyltransferase 
involved in cell envelope biogenesis 
[Cell envelope biogenesis, outer membrane]
CD-Length = 314 residues,  99.7% aligned
Score =  419 bits (1079), Expect = 4e-118

pfam01795, Methyltransf_5, MraW methylase family. 
CD-Length = 310 residues,  99.7% aligned
Score =  368 bits (947), Expect = 7e-103
Identities = 167/312 (53%), Positives = 219/312 (70%), Gaps = 7/312 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4797




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21943




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31462




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2397_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2397
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4048
                           PA4420_tr
                           translation of PA4420
                           313
                           99.36
                           99.36
                           0
                           1570.0
     

     
     
121479
                           gb|AAG07808.1|AE004856_19
                           (AE004856) conserved hypothetical protein [Pseudomonas aeruginosa PAO1]
                           313
                           99.36
                           99.36
                           0
                           1570.0
     

     
     
121480
                           gb|AAN66952.1|AE016324_2
                           (AE016779) conserved hypothetical protein TIGR00006 [Pseudomonas putida KT2440]
                           315
                           81.90
                           89.84
                           2
                           1317.0
     

     
     
121481
                           gb|AAO57865.1|
                           (AE016871) S-adenosyl-methyltransferase MraW [Pseudomonas syringae pv. tomato str. DC3000]
                           313
                           80.83
                           89.45
                           0
                           1294.0
     

     
     
121491
                           emb|CAD16559.1|
                           (AL646072) CONSERVED HYPOTHETICAL PROTEIN [Ralstonia solanacearum]
                           324
                           56.48
                           70.98
                           16
                           841.0
     

     






      

          
GC ORFID: 45399How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4420Glimmer Score: 

          
Start: 4382808Stop: 4381867

          
Length: 942


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4420 translation of PA4420Homolog Bit Score 609.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene2038 (ORF: BLASTX 504.0), t_PA4420 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAATGCCACCTACCGCCATATCACCGTTCTGCTCGAGGAGGCCGTTTCCGCACTGGCCCCGCGGGAGGAC 
GGCTGCTATCTGGACGGTACCTTCGGAAGAGGGGGCCATAGTCGAGCCTTGCTCGAGAAACTGGGCACCGGG 
GGACGCCTGCTCGGATTCGACAAAGATCCCCAGGCCATCCAGACAGGAAAAGCATTGGCGGCCGAAGACGGC 
CGCTTCGTCATTGTGCAGCGCTCGTTCGCCGAACTGGGCGATGAGGTGCGCGCACGCGGTCTCGAAGGTCGG 
GTCGATGGCGTCCTGCTCGACCTTGGCGTGTCGTCGCCGCAACTCGACGATCCCGAGCGCGGCTTCAGCTTC 
CTCAACGATGGTCCCCTGGACATGCGCATGAATCCCGGCCAGGGCATCAGCGCAGCGGAGTTCATCGCCAAC 
GCCACCGAAGAGGAAATCGCCCGGGTCTTCAAGGTATATGGCGAAGAGCGCTTCGCCAAGCGGATGGCCCGC 
GCCATTGTGCAGCGCCGTCAGGAGCGGCCGTTCGAACGGACCGCTGATCTCGCCGAAGTGATCACGGTTGCC 
AACCCCGCCTGGGAGAAGGGCAAGAATCCGGCGACCCGCGCATTCCAGGGCCTGCGCATCCATGTGAACAAT 
GAATTGGGCGATCTCGAGCGCGGCCTTGACGCCGCGCTCGAATCGCTGGCCGTCGGCGGCCGCCTGGTGGTG 
ATCAGTTTCCATTCCCTGGAAGACCGCATCGTCAAGCTGTTCATGCGCAAGCACGCCAAGGGCGAGGCGGAC 
AACCTGCCGCGCGACCTGCCGATCCGTTCCAAGGTCTTCGAGCCGCGCCTGAAGCTGCTGGGCAAGCCGCAG 
TACGCTTCGGAAGAAGAACTCAAGGCCAACCCGCGCTCGCGCAGCGCGGTCATGCGCGTGGCGGAGAAGCTG 
AGATGA