ViewGene
Locus Name:
GeneID:
Location:

GeneID:2385
TrivialName: PA14_18610
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_18610
PAO1 Ortholog Locus:PA3537
Sequence Length:918
Protein Length:305
Start: 1599101
Stop: 1598184
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06925.1|AE004774_3 (AE004774) ornithine carbamoyltransferase, anabolic [Pseudomonas aeruginosa PAO1]
Identities = 305/305 (100%), Positives = 305/305 (100%)
Sequence:
ATGAGCGTACGGCACTTTCTCTCGTTTATGGATTACAGCCCCGAAGAGCTGATCGGTCTGATCCGCCGCGGC
AGCGAGCTGAAGGACCTGCGCAACCGAGGCGTGCTCTACGAGCCTCTGAAGAGCCGGGTCCTGGGCATGGTC
TTCGAGAAGGCTTCGACCCGTACCCGCCTGTCCTTCGAAGCCGGGATGATCCAGCTCGGCGGCCAGGCGATC
TTCCTCTCCCCGCGCGACACCCAGCTCGGCCGCGGCGAGCCGATCGGCGACAGCGCCCGGGTCATGTCGCGG
ATGCTCGACGGCGTGATGATCCGCACCTTCGCCCACGCCACCCTGACGGAATTCGCCGCGCACTCGAAGGTG
CCGGTGATCAACGGCCTGTCCGACGACCTGCACCCCTGCCAGTTGCTCGCCGACATGCAGACCTTCCACGAG
CACCGCGGCAGCATCCAGGGCAAGACCGTGGCCTGGATCGGCGACGGCAACAACATGTGCAACAGCTATATA
GAAGCGGCGCTGAAGTTCGACTTCCAGCTGCGCGTGGCCTGCCCCGAGGGCTACGAGCCGAAGGCCGAGTTC
GTCGCCCTTGCCGGCGACCGCCTGCGCGTCGTCCGCGACCCGCGCGAGGCGGTGGCCGGCGCGCACCTGGTG
AGCACCGACGTGTGGGCCTCGATGGGCCAGGAAGACGAAGCCGCCGCGCGCATCGCCCTGTTCCGTCCCTAC
CAGGTGAACGCCGCGTTGCTCGATGGGGCCGCCGACGATGTACTATTCATGCACTGCCTGCCGGCCCACCGC
GGCGAGGAAATCAGCGAAGAGTTGCTGGACGACCCGCGCTCGGTAGCCTGGGACCAGGCAGAGAACCGTCTG
CACGCGCAGAAGGCCCTCCTCGAATTGCTGATCGAACACGCCCATTACGCCTGA
Translation:
MSVRHFLSFMDYSPEELIGLIRRGSELKDLRNRGVLYEPLKSRVLGMVFEKASTRTRLSFEAGMIQLGGQAI
FLSPRDTQLGRGEPIGDSARVMSRMLDGVMIRTFAHATLTEFAAHSKVPVINGLSDDLHPCQLLADMQTFHE
HRGSIQGKTVAWIGDGNNMCNSYIEAALKFDFQLRVACPEGYEPKAEFVALAGDRLRVVRDPREAVAGAHLV
STDVWASMGQEDEAAARIALFRPYQVNAALLDGAADDVLFMHCLPAHRGEEISEELLDDPRSVAWDQAENRL
HAQKALLELLIEHAHYA*
AnnotationID:2393GeneID:2385
AnnotatorUID: diggins
Modification Date/Time: 2005-04-19 17:05:54
Gene Name:argF
Confidence Code:1
GeneProduct:ornithine carbamoyltransferase, anabolic
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:aspartate/ornithine carbamoyltransferase
Functional Category Confidence Code:5
COGs:pfam02729,pfam00185,COG0078,COG0540
Secondary Functional Category(ies):
EC Number:2.1.3.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57620.1| (AE016871) ornithine carbamoyltransferase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 306

 Score =  503 bits (1294), Expect = e-142
 Identities = 248/299 (82%), Positives = 272/299 (90%)

>gb|AAN66704.1|AE016298_8 (AE016778) ornithine carbamoyltransferase [Pseudomonas putida
           KT2440]
          Length = 306

 Score =  485 bits (1248), Expect = e-136
 Identities = 237/304 (77%), Positives = 268/304 (88%)

>gb|AAB89987.1| (AE001017) ornithine carbamoyltransferase (argF) [Archaeoglobus
           fulgidus DSM 4304]
          Length = 307

 Score =  308 bits (789), Expect = 3e-83
 Identities = 154/300 (51%), Positives = 212/300 (70%), Gaps = 3/300 (1%)






 
Structural Features:
pfam02729, OTCace_N, Aspartate/ornithine carbamoyltransferase, 
carbamoyl-P binding domain.
CD-Length = 143 residues,  99.3% aligned
Score =  193 bits (493), Expect = 2e-50
Identities = 68/142 (47%), Positives = 87/142 (61%)

COG0078, ArgF, Ornithine carbamoyltransferase 
[Amino acid transport and metabolism]
CD-Length = 310 residues,  99.0% aligned
Score =  432 bits (1113), Expect = 2e-122
Identities = 162/308 (52%), Positives = 212/308 (68%), Gaps = 9/308 (2%)

COG0540, PyrB, Aspartate carbamoyltransferase, catalytic chain 
[Nucleotide biosythesis and metabolism]
CD-Length = 316 residues,  96.2% aligned
Score =  150 bits (381), Expect = 2e-37
Identities = 93/308 (30%), Positives = 148/308 (48%), Gaps = 16/308 (5%)

pfam00185, OTCace, Aspartate/ornithine carbamoyltransferase,
Asp/Orn binding domain.
CD-Length = 160 residues, 100.0% aligned
Score =  171 bits (434), Expect = 1e-43
Identities = 79/160 (49%), Positives = 101/160 (63%), Gaps = 9/160 (5%)







   
Genomic Context:






   
Comment:


















    ReferenceID:4773




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21919




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32027




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2385_cdd.html


    		




 
URL:

















    ReferenceID:34124




  
Author/Investigator(s):
        toh Y, Soldati L, Stalon V, Falmagne P, Terawaki Y, Leisinger T, Haas D.





  
Title:
        Anabolic ornithine carbamoyltransferase of Pseudomonas aeruginosa: nucleotide sequence and transcriptional control of the argF structural gene.




  
PubMed:
        3131308


MedLine:
        




    
Source:
J Bacteriol. 1988 Jun;170(6):2725-34.

Erratum in:
    * J Bacteriol 1990 Apr;172(4):2199.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3131308

















    ReferenceID:34125




  
Author/Investigator(s):
        Haas D, Holloway BW, Schambock A, Leisinger T.





  
Title:
        The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa.




  
PubMed:
        408599


MedLine:
        




    
Source:
Mol Gen Genet. 1977 Jul 7;154(1):7-22.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=408599









           
Homologs By Global Alignment 

           

       



     
Gene ID:2385
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4031
                           PA3537_tr
                           translation of PA3537
                           305
                           99.99
                           99.99
                           0
                           1573.0
     

     
     
121126
                           gb|AAG06925.1|AE004774_3
                           (AE004774) ornithine carbamoyltransferase, anabolic [Pseudomonas aeruginosa PAO1]
                           305
                           99.99
                           99.99
                           0
                           1573.0
     

     
     
121127
                           gb|AAA25720.1|
                           (M19939) ornithine carbamoyltransferase (EC 2.1.3.3) [Pseudomonas aeruginosa]
                           305
                           98.68
                           99.34
                           0
                           1559.0
     

     
     
121128
                           gb|AAO57620.1|
                           (AE016871) ornithine carbamoyltransferase [Pseudomonas syringae pv. tomato str. DC3000]
                           306
                           81.04
                           88.88
                           1
                           1288.0
     

     
     
121129
                           emb|CAA54264.1|
                           (X76945) orthinine carbamoyltransferase [Pseudomonas syringae]
                           306
                           80.39
                           89.21
                           1
                           1288.0
     

     






      

          
GC ORFID: 45387How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3537Glimmer Score: 

          
Start: 3354833Stop: 3355750

          
Length: 918


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3537 translation of PA3537Homolog Bit Score 610.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6123 (ORF: BLASTX 505.0), t_PA3537 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGTACGGCACTTTCTCTCGTTTATGGATTACAGCCCCGAAGAGCTGATCGGTCTGATCCGCCGCGGC 
AGCGAGCTGAAGGACCTGCGCAACCGAGGCGTGCTCTACGAGCCTCTGAAGAGCCGGGTCCTGGGCATGGTC 
TTCGAGAAGGCTTCGACCCGTACCCGCCTGTCCTTCGAAGCCGGGATGATCCAGCTCGGCGGCCAGGCGATC 
TTCCTCTCCCCGCGCGACACCCAGCTCGGCCGCGGCGAGCCGATCGGCGACAGCGCCCGGGTCATGTCGCGG 
ATGCTCGACGGCGTGATGATCCGCACCTTCGCCCACGCCACCCTGACGGAATTCGCCGCGCACTCGAAGGTG 
CCGGTGATCAACGGCCTGTCCGACGACCTGCACCCCTGCCAGTTGCTCGCCGACATGCAGACCTTCCACGAG 
CACCGCGGCAGCATCCAGGGCAAGACCGTGGCCTGGATCGGCGACGGCAACAACATGTGCAACAGCTATATA 
GAAGCGGCGCTGAAGTTCGACTTCCAGCTGCGCGTGGCCTGCCCCGAGGGCTACGAGCCGAAGGCCGAGTTC 
GTCGCCCTTGCCGGCGACCGCCTGCGCGTCGTCCGCGACCCGCGCGAGGCGGTGGCCGGCGCGCACCTGGTG 
AGCACCGACGTGTGGGCCTCGATGGGCCAGGAAGACGAAGCCGCCGCGCGCATCGCCCTGTTCCGTCCCTAC 
CAGGTGAACGCCGCGTTGCTCGATGGGGCCGCCGACGATGTACTATTCATGCACTGCCTGCCGGCCCACCGC 
GGCGAGGAAATCAGCGAAGAGTTGCTGGACGACCCGCGCTCGGTAGCCTGGGACCAGGCAGAGAACCGTCTG 
CACGCGCAGAAGGCCCTCCTCGAATTGCTGATCGAACACGCCCATTACGCCTGA