ViewGene
Locus Name:
GeneID:
Location:

GeneID:2368
TrivialName: PA14_27100
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_27100
PAO1 Ortholog Locus:PA2862
Sequence Length:936
Protein Length:311
Start: 2355101
Stop: 2354166
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06250.1|AE004712_10 (AE004712) lactonizing lipase precursor [Pseudomonas aeruginosa PAO1]
Identities = 311/311 (100%), Positives = 311/311 (100%)
Sequence:
ATGAAGAAGAAGTCTCTGCTCCCCCTCGGCCTGGCCATCGGCCTCGCCTCTCTCGCTGCCAGCCCTCTGATC
CAGGCCAGCACCTACACCCAGACCAAATACCCCATCGTGCTGGCCCACGGCATGCTCGGCTTCGACAACATC
CTCGGGGTCGACTACTGGTTCGGCATTCCCAGCGCCTTGCGCCGTGACGGTGCCCAGGTCTACGTCACCGAA
GTCAGCCAGTTGGACACCTCGGAAGTCCGCGGCGAGCAGTTGCTGCAACAGGTGGAGGAAATCGTCGCCCTC
AGCGGCCAGCCCAAGGTCAACCTGATCGGCCACAGCCACGGCGGGCCGACCATCCGCTACGTCGCCGCCGTA
CGTCCCGACCTGATCGCTTCCGCCACCAGCGTCGGCGCCCCGCACAAGGGTTCGGACACCGCCGACTTCCTG
CGCCAGATCCCTCCGGGTTCGGCCGGCGAGGCAGTCCTCTCCGGGCTGGTCAACAGCCTCGGCGCGCTGATC
AGCTTCCTTTCCAGCGGCAGCACCGGTACGCAGAATTCACTGGGCTCGCTGGAGTCGCTGAACAGCGAGGGT
GCCGCGCGCTTCAACGCCAAGTACCCGCAGGGCATCCCCACCTCGGCCTGCGGCGAAGGCGCCTACAAGGTC
AACGGCGTGAGCTATTACTCCTGGAGCGGTTCCTCGCCGCTGACCAACTTCCTCGATCCGAGCGACGCCTTC
CTCGGCGCCTCGTCGCTGACCTTCAAGAACGGCACCGCCAACGACGGCCTGGTCGGCACCTGCAGTTCGCAC
CTGGGCATGGTGATCCGCGACAACTACCGGATGAACCACCTGGACGAGGTGAACCAGGTCTTCGGCCTCACC
AGCCTGTTCGAGACCAGCCCGGTCAGCGTCTACCGCCAGCACGCCAACCGCCTGAAGAACGCCAGCCTGTAG
Translation:
MKKKSLLPLGLAIGLASLAASPLIQASTYTQTKYPIVLAHGMLGFDNILGVDYWFGIPSALRRDGAQVYVTE
VSQLDTSEVRGEQLLQQVEEIVALSGQPKVNLIGHSHGGPTIRYVAAVRPDLIASATSVGAPHKGSDTADFL
RQIPPGSAGEAVLSGLVNSLGALISFLSSGSTGTQNSLGSLESLNSEGAARFNAKYPQGIPTSACGEGAYKV
NGVSYYSWSGSSPLTNFLDPSDAFLGASSLTFKNGTANDGLVGTCSSHLGMVIRDNYRMNHLDEVNQVFGLT
SLFETSPVSVYRQHANRLKNASL*
AnnotationID:2376GeneID:2368
AnnotatorUID: diggins
Modification Date/Time: 2005-04-07 06:06:43
Gene Name:lipA
Confidence Code:1
GeneProduct:lactonizing lipase precursor
Cell Localization:()
Synonyms:libA
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(23) Secreted Factors (toxins, enzymes, alginate)
Alternate Gene Product Name:triacylglycerol lipase
Functional Category Confidence Code:5
COGs:COG1075
Secondary Functional Category(ies):
EC Number:3.1.1.3
Status:ACTIVE
Pathway:






 
Homology:
>dbj|BAA01035.1| (D10166) lipase precursor [Pseudomonas sp.]
          Length = 311

 Score =  608 bits (1567), Expect = e-173
 Identities = 308/311 (99%), Positives = 309/311 (99%)

>gb|AAM14701.1| (AY091666) LipA [Pseudomonas mendocina]
          Length = 311

 Score =  473 bits (1218), Expect = e-133
 Identities = 238/311 (76%), Positives = 266/311 (85%), Gaps = 3/311 (0%)

>gb|AAF96133.1| (AE004362) lactonizing lipase [Vibrio cholerae]
          Length = 339

 Score =  363 bits (932), Expect = e-100
 Identities = 184/312 (58%), Positives = 228/312 (73%), Gaps = 6/312 (1%)






 
Structural Features:
COG1075, LipA, Predicted acetyltransferases and hydrolases with the 
alpha/beta hydrolase fold
CD-Length = 336 residues,  92.3% aligned
Score =  219 bits (559), Expect = 4e-58
Identities = 109/319 (34%), Positives = 146/319 (45%), Gaps = 20/319 (6%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4739




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21885




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36204




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2368_cdd.html


    		




 
URL:

















    ReferenceID:36407




  
Author/Investigator(s):
        Wohlfarth S, Hoesche C, Strunk C, Winkler UK.





  
Title:
        Molecular genetics of the extracellular lipase of Pseudomonas aeruginosa PAO1.




  
PubMed:
        1512563


MedLine:
        




    
Source:
J Gen Microbiol. 1992 Jul;138 ( Pt 7):1325-35.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1512563









           
Homologs By Global Alignment 

           

       



     
Gene ID:2368
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
4010
                           PA2862_tr
                           translation of PA2862
                           311
                           99.99
                           99.99
                           0
                           1580.0
     

     
     
120985
                           gb|AAG06250.1|AE004712_10
                           (AE004712) lactonizing lipase precursor [Pseudomonas aeruginosa PAO1]
                           311
                           99.99
                           99.99
                           0
                           1580.0
     

     
     
120986
                           emb|CAA44997.1|
                           (X63390) triacylglycerol lipase [Pseudomonas aeruginosa]
                           311
                           99.99
                           99.99
                           0
                           1580.0
     

     
     
120987
                           dbj|BAA09135.1|
                           (D50587) lipase [Pseudomonas aeruginosa]
                           311
                           99.35
                           99.99
                           0
                           1578.0
     

     
     
120988
                           gb|AAF64156.1|AF237723_1
                           (AF237723) triacylglycerol acylhydrolase [Pseudomonas aeruginosa]
                           311
                           99.35
                           99.99
                           0
                           1576.0
     

     






      

          
GC ORFID: 45370How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2862Glimmer Score: 

          
Start: 2598825Stop: 2599760

          
Length: 936


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA2862 translation of PA2862Homolog Bit Score 613.0

          
Other Homologs:  t_PA4813 (ORF: BLASTX 325.0), t_PA2862 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAGAAGAAGTCTCTGCTCCCCCTCGGCCTGGCCATCGGCCTCGCCTCTCTCGCTGCCAGCCCTCTGATC 
CAGGCCAGCACCTACACCCAGACCAAATACCCCATCGTGCTGGCCCACGGCATGCTCGGCTTCGACAACATC 
CTCGGGGTCGACTACTGGTTCGGCATTCCCAGCGCCTTGCGCCGTGACGGTGCCCAGGTCTACGTCACCGAA 
GTCAGCCAGTTGGACACCTCGGAAGTCCGCGGCGAGCAGTTGCTGCAACAGGTGGAGGAAATCGTCGCCCTC 
AGCGGCCAGCCCAAGGTCAACCTGATCGGCCACAGCCACGGCGGGCCGACCATCCGCTACGTCGCCGCCGTA 
CGTCCCGACCTGATCGCTTCCGCCACCAGCGTCGGCGCCCCGCACAAGGGTTCGGACACCGCCGACTTCCTG 
CGCCAGATCCCTCCGGGTTCGGCCGGCGAGGCAGTCCTCTCCGGGCTGGTCAACAGCCTCGGCGCGCTGATC 
AGCTTCCTTTCCAGCGGCAGCACCGGTACGCAGAATTCACTGGGCTCGCTGGAGTCGCTGAACAGCGAGGGT 
GCCGCGCGCTTCAACGCCAAGTACCCGCAGGGCATCCCCACCTCGGCCTGCGGCGAAGGCGCCTACAAGGTC 
AACGGCGTGAGCTATTACTCCTGGAGCGGTTCCTCGCCGCTGACCAACTTCCTCGATCCGAGCGACGCCTTC 
CTCGGCGCCTCGTCGCTGACCTTCAAGAACGGCACCGCCAACGACGGCCTGGTCGGCACCTGCAGTTCGCAC 
CTGGGCATGGTGATCCGCGACAACTACCGGATGAACCACCTGGACGAGGTGAACCAGGTCTTCGGCCTCACC 
AGCCTGTTCGAGACCAGCCCGGTCAGCGTCTACCGCCAGCACGCCAACCGCCTGAAGAACGCCAGCCTGTAG