ViewGene
Locus Name:
GeneID:
Location:

GeneID:2356
TrivialName: PA14_66260
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_66260
PAO1 Ortholog Locus:PA5013
Sequence Length:924
Protein Length:307
Start: 5904267
Stop: 5903344
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08398.1|AE004913_13 (AE004913) branched-chain amino acid transferase [Pseudomonas aeruginosa PAO1]
Identities = 307/307 (100%), Positives = 307/307 (100%)
Sequence:
ATGTCGATGGCCGATCGTGATGGCGTGATCTGGTATGACGGTGAACTGGTGCAGTGGCGCGACGCGACCACG
CACGTGCTGACCCATACCCTGCACTATGGAATGGGCGTGTTCGAGGGCGTGCGCGCCTACGACACCCCGCAG
GGCACGGCGATCTTCCGCCTGCAGGCGCACACTGACCGGCTGTTCGACTCCGCGCACATCATGAACATGCAG
ATCCCGTACAGCCGCGACGAGATCAACGAGGCGACCCGCGCCGCCGTGCGCGAGAACAACCTGGAAAGCGCC
TATATCCGCCCGATGGTGTTCTACGGAAGCGAAGGCATGGGCCTGCGCGCCAGCGGCCTGAAGGTCCATGTG
ATCATCGCCGCCTGGAGCTGGGGCGCCTACATGGGCGAGGAAGCCCTGCAGCAAGGCATCAAGGTGCGCACC
AGTTCCTTCACCCGCCACCACGTCAACATCTCGATGACCCGCGCCAAGTCCAACGGCGCCTACATCAACTCG
ATGCTGGCCCTCCAGGAAGCGATCTCCGGCGGCGCCGACGAGGCCATGATGCTCGATCCGGAAGGCTATGTG
GCCGAAGGCTCCGGCGAGAACATCTTCATCATCAAGGATGGCGTGATCTACACCCCGGAAGTCACCGCCTGC
CTGAACGGCATCACTCGTAACACTATCCTGACCCTGGCCGCCGAACACGGTTTTAAACTGGTCGAGAAGCGC
ATCACCCGCGACGAGGTGTACATCGCCGACGAGGCCTTCTTCACCGGCACTGCCGCGGAAGTCACGCCGATC
CGCGAAGTGGACGGTCGCAAGATCGGCGCCGGCCGCCGTGGCCCGGTCACCGAAAAGCTGCAGAAAGCCTAT
TTCGACCTGGTCAGCGGCAAGACCGAGGCCCACGCCGAGTGGCGTACCCTGGTCAAGTAA
Translation:
MSMADRDGVIWYDGELVQWRDATTHVLTHTLHYGMGVFEGVRAYDTPQGTAIFRLQAHTDRLFDSAHIMNMQ
IPYSRDEINEATRAAVRENNLESAYIRPMVFYGSEGMGLRASGLKVHVIIAAWSWGAYMGEEALQQGIKVRT
SSFTRHHVNISMTRAKSNGAYINSMLALQEAISGGADEAMMLDPEGYVAEGSGENIFIIKDGVIYTPEVTAC
LNGITRNTILTLAAEHGFKLVEKRITRDEVYIADEAFFTGTAAEVTPIREVDGRKIGAGRRGPVTEKLQKAY
FDLVSGKTEAHAEWRTLVK*
AnnotationID:2364GeneID:2356
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 09:09:55
Gene Name:ilvE
Confidence Code:2
GeneProduct:branched-chain amino acid transferase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:cd00449,pfam01063,COG0115
Secondary Functional Category(ies):central intermediary metabolism
EC Number:2.6.1.42
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAD85804.1| (BX321862) Aminotransferases class-IV [Nitrosomonas europaea ATCC
           19718]
          Length = 307

 Score =  430 bits (1105), Expect = e-120
 Identities = 205/302 (67%), Positives = 246/302 (81%)

>emb|CAE34349.1| (BX640449) branched-chain amino acid aminotransferase [Bordetella
           bronchiseptica]
          Length = 306

 Score =  422 bits (1084), Expect = e-117
 Identities = 202/306 (66%), Positives = 245/306 (80%)

>emb|CAD14097.1| (AL646060) PROBABLE BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE
           PROTEIN [Ralstonia solanacearum]
          Length = 309

 Score =  414 bits (1063), Expect = e-115
 Identities = 199/306 (65%), Positives = 246/306 (80%), Gaps = 2/306 (0%)








 
Structural Features:
cd00449, PLPDE_IV, PyridoxaL 5'-Phosphate Dependent Enzymes class IV (PLPDE_IV).
CD-Length = 279 residues,  99.6% aligned
Score =  284 bits (729), Expect = 7e-78
Identities = 122/281 (43%), Positives = 176/281 (62%), Gaps = 5/281 (1%)

pfam01063, Aminotran_4, Aminotransferase class IV.
CD-Length = 276 residues, 100.0% aligned
Score =  266 bits (681), Expect = 3e-72
Identities = 121/280 (43%), Positives = 170/280 (60%), Gaps = 7/280 (2%)

COG0115, IlvE, Branched-chain amino acid 
aminotransferase/4-amino-4-deoxychorismate lyase 
[Amino acid transport and metabolism / Coenzyme metabolism]
CD-Length = 284 residues, 100.0% aligned
Score =  266 bits (682), Expect = 2e-72
Identities = 123/293 (41%), Positives = 175/293 (59%), Gaps = 12/293 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4715




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21861




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35060




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2356_cdd.html


    		




 
URL:

















    ReferenceID:35869




  
Author/Investigator(s):
        Kuramitsu S, Ogawa T, Ogawa H, Kagamiyama H.





  
Title:
        Branched-chain amino acid aminotransferase of Escherichia coli: nucleotide sequence of the ilvE gene and the deduced amino acid sequence.




  
PubMed:
        3897211


MedLine:
        




    
Source:
J Biochem (Tokyo). 1985 Apr;97(4):993-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=3897211









           
Homologs By Global Alignment 

           

       



     
Gene ID:2356
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3998
                           PA5013_tr
                           translation of PA5013
                           307
                           99.99
                           99.99
                           0
                           1590.0
     

     
     
120769
                           gb|AAG08398.1|AE004913_13
                           (AE004913) branched-chain amino acid transferase [Pseudomonas aeruginosa PAO1]
                           307
                           99.99
                           99.99
                           0
                           1590.0
     

     
     
120770
                           gb|AAC33172.1|
                           (U63816) unknown [Pseudomonas aeruginosa]
                           307
                           93.15
                           94.13
                           0
                           1468.0
     

     
     
120771
                           emb|CAD85804.1|
                           (BX321862) Aminotransferases class-IV [Nitrosomonas europaea ATCC 19718]
                           307
                           66.77
                           80.13
                           0
                           1099.0
     

     
     
120772
                           emb|CAE34349.1|
                           (BX640449) branched-chain amino acid aminotransferase [Bordetella bronchiseptica] [Bordetella bronchiseptica RB50]
                           307
                           65.79
                           79.80
                           1
                           1084.0
     

     






      

          
GC ORFID: 45358How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5013Glimmer Score: 

          
Start: 5167950Stop: 5167027

          
Length: 924


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA5013 translation of PA5013Homolog Bit Score 617.0

          
Other Homologs:  t_PA5013 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGATGGCCGATCGTGATGGCGTGATCTGGTATGACGGTGAACTGGTGCAGTGGCGCGACGCGACCACG 
CACGTGCTGACCCATACCCTGCACTATGGAATGGGCGTGTTCGAGGGCGTGCGCGCCTACGACACCCCGCAG 
GGCACGGCGATCTTCCGCCTGCAGGCGCACACTGACCGGCTGTTCGACTCCGCGCACATCATGAACATGCAG 
ATCCCGTACAGCCGCGACGAGATCAACGAGGCGACCCGCGCCGCCGTGCGCGAGAACAACCTGGAAAGCGCC 
TATATCCGCCCGATGGTGTTCTACGGAAGCGAAGGCATGGGCCTGCGCGCCAGCGGCCTGAAGGTCCATGTG 
ATCATCGCCGCCTGGAGCTGGGGCGCCTACATGGGCGAGGAAGCCCTGCAGCAAGGCATCAAGGTGCGCACC 
AGTTCCTTCACCCGCCACCACGTCAACATCTCGATGACCCGCGCCAAGTCCAACGGCGCCTACATCAACTCG 
ATGCTGGCCCTCCAGGAAGCGATCTCCGGCGGCGCCGACGAGGCCATGATGCTCGATCCGGAAGGCTATGTG 
GCCGAAGGCTCCGGCGAGAACATCTTCATCATCAAGGATGGCGTGATCTACACCCCGGAAGTCACCGCCTGC 
CTGAACGGCATCACTCGTAACACTATCCTGACCCTGGCCGCCGAACACGGTTTTAAACTGGTCGAGAAGCGC 
ATCACCCGCGACGAGGTGTACATCGCCGACGAGGCCTTCTTCACCGGCACTGCCGCGGAAGTCACGCCGATC 
CGCGAAGTGGACGGTCGCAAGATCGGCGCCGGCCGCCGTGGCCCGGTCACCGAAAAGCTGCAGAAAGCCTAT 
TTCGACCTGGTCAGCGGCAAGACCGAGGCCCACGCCGAGTGGCGTACCCTGGTCAAGTAA