ViewGene
Locus Name:
GeneID:
Location:

GeneID:2353
TrivialName: PA14_58550
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_58550
PAO1 Ortholog Locus:PA4512
Sequence Length:900
Protein Length:299
Start: 5213357
Stop: 5214256
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07900.1|AE004865_1 (AE004865) lipopolysaccharide biosynthetic protein LpxO1 [Pseudomonas aeruginosa PAO1]
Identities = 297/299 (99%), Positives = 297/299 (99%)
Sequence:
ATGAAAGTTCTCATCCTGGCGGCCTATGCGCTGAGCATCCTCTATGTCCATTTTCGCGGCCGGGTACGCCAC
AAGCTGTCGCGCCAATTGAGCGACCACTCCAGCTTCCTCGCTCCGATCAACTGCCTGATGTACCTGTTCTCG
AAGGTAGCGAACCGCCCCTACCTGGCACCGCAGACCTTCCCCGAACTGCAGGTTCTGGTCGACAACTGGCAG
GCCATCCGCAGCGAGGCGCTGCACCTGCACGGCGCCGGCAACATCAAGCGTTCCGAACAGTACGACGATATC
GGTTTCAACTCGTTCTTCAAGAGCGGCTGGAAGCGCTTCTACCTGAAATGGTACGACGAGGCCCATCCCTCC
GCCGGACAACTCTGCCCGCAGACCACCGCCCTGTTGCGGCAGATTCCCTCGGTCAAGGCGGCGATGTTCGCC
GAACTGCCGCCGGGCTCGAAGCTGGTGCGACACCGCGATCCCTACGCCGGCTCGCTGCGCTTCCACCTGGGA
CTGTTCACTCCCAACGACCCGGCCTGCTTCATCGAGGTGGACGGCGAGCGCTACCACTGGCGCGACGGCGAG
GCGGTGGTGTTCGATGAAACCTTCATCCACTACGCGGAAAACGCCACCGAACACAACCGCATCATTCTCTTC
TGCGACATCGAGCGGCCGCTGACCCAGGGCTGGGCCACCGCCTTCAACCGCTGGTTCAGCCGCAACGTGATG
GCTGCCGCGGCCGCCCCGAACAACGCAGGCGACCGGACCGGCGGACTCAATCGGATCTTCGACAGCCTCTAC
AAGATCCGCCTGCAGGGCAAACGTTTGAAGAAGCGCAACCGCAAGCTCTACTACCTGCAGAAATGGGGCTTC
TTCGCCCTGGTACTGGCTTTCATCCTGTGGATCTGA
Translation:
MKVLILAAYALSILYVHFRGRVRHKLSRQLSDHSSFLAPINCLMYLFSKVANRPYLAPQTFPELQVLVDNWQ
AIRSEALHLHGAGNIKRSEQYDDIGFNSFFKSGWKRFYLKWYDEAHPSAGQLCPQTTALLRQIPSVKAAMFA
ELPPGSKLVRHRDPYAGSLRFHLGLFTPNDPACFIEVDGERYHWRDGEAVVFDETFIHYAENATEHNRIILF
CDIERPLTQGWATAFNRWFSRNVMAAAAAPNNAGDRTGGLNRIFDSLYKIRLQGKRLKKRNRKLYYLQKWGF
FALVLAFILWI*
AnnotationID:2361GeneID:2353
AnnotatorUID: danlee
Modification Date/Time: 2006-03-29 14:02:23
Gene Name:lpxO1
Confidence Code:2
GeneProduct:lipopolysaccharide biosynthetic protein LpxO1
Cell Localization:()
Synonyms:PA4512
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(7) Cell wall / LPS / capsule
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG3555,pfam05118
Secondary Functional Category(ies):posttranslational modification, protein turnover, chaperones
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAM39701.1| (AE012134) aspartyl/asparaginyl beta-hydroxylase [Xanthomonas
           campestris pv. campestris str. ATCC 33913]
          Length = 301

 Score =  382 bits (981), Expect = e-105
 Identities = 181/298 (60%), Positives = 225/298 (75%), Gaps = 1/298 (0%)

>gb|AAM35274.1| (AE011664) aspartyl-asparaginyl beta-hydroxylase [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 301

 Score =  382 bits (980), Expect = e-105
 Identities = 180/298 (60%), Positives = 226/298 (75%), Gaps = 1/298 (0%)






 
Structural Features:
COG3555, Aspartyl/asparaginyl beta-hydroxylase and related dioxygenases 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 291 residues, 100.0% aligned
Score =  337 bits (865), Expect = 2e-93
Identities = 141/293 (48%), Positives = 167/293 (56%), Gaps = 4/293 (1%)

pfam05118, Asp_Arg_Hydrox, Aspartyl/Asparaginyl beta-hydroxylase.
CD-Length = 163 residues,  99.4% aligned
Score =  214 bits (545), Expect = 3e-56
Identities = 85/163 (52%), Positives = 105/163 (64%), Gaps = 7/163 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4709




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21855




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31556




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2353_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2353
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3994
                           PA4512_tr
                           translation of PA4512
                           299
                           99.33
                           99.33
                           0
                           1590.0
     

     
     
120708
                           gb|AAG07900.1|AE004865_1
                           (AE004865) lipopolysaccharide biosynthetic protein LpxO1 [Pseudomonas aeruginosa PAO1]
                           299
                           99.33
                           99.33
                           0
                           1590.0
     

     
     
120709
                           gb|AAN68035.1|AE016436_3
                           (AE016783) conserved hypothetical protein [Pseudomonas putida KT2440]
                           300
                           68.66
                           80.00
                           2
                           1116.0
     

     
     
120711
                           gb|AAM39701.1|
                           (AE012134) aspartyl/asparaginyl beta-hydroxylase [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           301
                           60.13
                           75.08
                           2
                           983.0
     

     
     
120712
                           gb|AAM35274.1|
                           (AE011664) aspartyl-asparaginyl beta-hydroxylase [Xanthomonas axonopodis pv. citri str. 306]
                           301
                           59.80
                           75.41
                           2
                           982.0
     

     






      

          
GC ORFID: 45355How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4512Glimmer Score: 

          
Start: 4477036Stop: 4477935

          
Length: 900


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4512 translation of PA4512Homolog Bit Score 617.0

          
Other Homologs:  t_PA4512 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAGTTCTCATCCTGGCGGCCTATGCGCTGAGCATCCTCTATGTCCATTTTCGCGGCCGGGTACGCCAC 
AAGCTGTCGCGCCAATTGAGCGACCACTCCAGCTTCCTCGCTCCGATCAACTGCCTGATGTACCTGTTCTCG 
AAGGTAGCGAACCGCCCCTACCTGGCACCGCAGACCTTCCCCGAACTGCAGGTTCTGGTCGACAACTGGCAG 
GCCATCCGCAGCGAGGCGCTGCACCTGCACGGCGCCGGCAACATCAAGCGTTCCGAACAGTACGACGATATC 
GGTTTCAACTCGTTCTTCAAGAGCGGCTGGAAGCGCTTCTACCTGAAATGGTACGACGAGGCCCATCCCTCC 
GCCGGACAACTCTGCCCGCAGACCACCGCCCTGTTGCGGCAGATTCCCTCGGTCAAGGCGGCGATGTTCGCC 
GAACTGCCGCCGGGCTCGAAGCTGGTGCGACACCGCGATCCCTACGCCGGCTCGCTGCGCTTCCACCTGGGA 
CTGTTCACTCCCAACGACCCGGCCTGCTTCATCGAGGTGGACGGCGAGCGCTACCACTGGCGCGACGGCGAG 
GCGGTGGTGTTCGATGAAACCTTCATCCACTACGCGGAAAACGCCACCGAACACAACCGCATCATTCTCTTC 
TGCGACATCGAGCGGCCGCTGACCCAGGGCTGGGCCACCGCCTTCAACCGCTGGTTCAGCCGCAACGTGATG 
GCTGCCGCGGCCGCCCCGAACAACGCAGGCGACCGGACCGGCGGACTCAATCGGATCTTCGACAGCCTCTAC 
AAGATCCGCCTGCAGGGCAAACGTTTGAAGAAGCGCAACCGCAAGCTCTACTACCTGCAGAAATGGGGCTTC 
TTCGCCCTGGTACTGGCTTTCATCCTGTGGATCTGA