ViewGene
Locus Name:
GeneID:
Location:

GeneID:2345
TrivialName: PA14_47440
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_47440
PAO1 Ortholog Locus:PA1296
Sequence Length:933
Protein Length:310
Start: 4227598
Stop: 4226666
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04685.1|AE004559_4 (AE004559) probable 2-hydroxyacid dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 308/310 (99%), Positives = 308/310 (99%)
Sequence:
ATGCGCTTGCTGATTCTCGAACGTGACCACGCTCTCTATGCCGCGCTGCTGATGGCGGCCGACCCCAGCCTG
AAGGTGGTGGCGGGGGACGACCCCCTGCAACTCATCGACGCCGCCAGCGAGTGCTCGATATGGCTCGGCCAG
CCCGACCTGGTGGCGCAGATGCTGCGCCAGGGCGTGCATCCGGTCTGGGTGCAGTCGACCTGGGCGGGCATC
ACGCCGCTGCTGGCCGCCGACCTGCCGAAGGACTACAGCCTGACACGCGCGGTAGGCATCTTCGGCCAGGTG
ATGAGCGAATACCTGCTGACCTACATGCTCGCCCACGAGCGCCAGTTCCTCGGTCGCCTGGCCAGCCAGGTC
GGTTCGCAATGGGATAGCCGCACGCCTGGCGGGTTGCGTGGCCGGCAGGCGGTGATCGTGGGTACCGGGGAG
ATCGGCCAGGCGGTGGCGCATACCCTGTCCGGCTTCGGCATGGACCTGACCGGGGTGGCGAAGAATCCGCGT
TCGCTGGTGCCGTTCAACCGCATGGGCTCGCTCGACGACCTCGGCCGCCTGGTGGAGACGGCCGACTACCTG
ATCAACCTGCTGCCCGACACCCCCGATACCCACGACATCTACGACCGTGCGCTGTTCGCCCGGCTCAAATCC
ACGGCGCTGTTCATCAACGCCGGGCGTGGCGTCGCGGTGGTGGACGCCGACCTGGTGGCGGCGCTGGAGAAC
AACCAGCTGGCCGGCGCGGTGATCGACGTCTGCCGCGAAGAGCCGCTGCCGGCCAACCACCTGTTCTGGCAT
ACCCCGCGCCTCTTGCTGACCGGCCACACCGCCGCGCCGACCCTGCCGGGGGCGATGGTCGAACTGTTCCGC
GACAACCTCGCGCGCTTCTGGGCGGGCACCGCCATGCGTGGCGAGGTGGACTTCGCTCGCGGCTACTGA
Translation:
MRLLILERDHALYAALLMAADPSLKVVAGDDPLQLIDAASECSIWLGQPDLVAQMLRQGVHPVWVQSTWAGI
TPLLAADLPKDYSLTRAVGIFGQVMSEYLLTYMLAHERQFLGRLASQVGSQWDSRTPGGLRGRQAVIVGTGE
IGQAVAHTLSGFGMDLTGVAKNPRSLVPFNRMGSLDDLGRLVETADYLINLLPDTPDTHDIYDRALFARLKS
TALFINAGRGVAVVDADLVAALENNQLAGAVIDVCREEPLPANHLFWHTPRLLLTGHTAAPTLPGAMVELFR
DNLARFWAGTAMRGEVDFARGY*
AnnotationID:2353GeneID:2345
AnnotatorUID: diggins
Modification Date/Time: 2005-05-25 13:01:31
Gene Name:
Confidence Code:3
GeneProduct:putative 2-hydroxyacid dehydrogenase
Cell Localization:()
Synonyms:PA1296
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0111,pfam02826
Secondary Functional Category(ies):energy metabolism
EC Number:1.1.1.272
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70162.1|AE016655_7 (AE016791) D-isomer specific 2-hydroxyacid dehydrogenase family
           protein [Pseudomonas putida KT2440]
          Length = 310

 Score =  379 bits (973), Expect = e-104
 Identities = 192/310 (61%), Positives = 227/310 (73%)

>gb|AAO57386.1| (AE016870) D-isomer specific 2-hydroxyacid dehydrogenase family
           protein [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 310

 Score =  368 bits (945), Expect = e-101
 Identities = 187/310 (60%), Positives = 225/310 (72%)

>gb|AAN53665.1|AE015505_8 (AE015505) D-isomer specific 2-hydroxyacid dehydrogenase family
           protein [Shewanella oneidensis MR-1]
          Length = 311

 Score =  189 bits (480), Expect = 2e-47
 Identities = 112/313 (35%), Positives = 172/313 (54%), Gaps = 9/313 (2%)







 
Structural Features:
COG0111, SerA, Phosphoglycerate dehydrogenase and related dehydrogenases 
[Amino acid transport and metabolism]
CD-Length = 324 residues,  99.7% aligned
Score =  205 bits (524), Expect = 4e-54
Identities = 95/323 (29%), Positives = 147/323 (45%), Gaps = 13/323 (4%)

pfam02826, 2-Hacid_dh_C, D-isomer specific 2-hydroxyacid dehydrogenase, 
NAD binding domain.
CD-Length = 184 residues,  98.9% aligned
Score =  102 bits (257), Expect = 3e-23
Identities = 51/183 (27%), Positives = 82/183 (44%), Gaps = 9/183 (4%)







   
Genomic Context:






   
Comment:


















    ReferenceID:4693




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21839




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37499




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2345_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2345
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3984
                           PA1296_tr
                           translation of PA1296
                           310
                           99.35
                           99.35
                           0
                           1592.0
     

     
     
120644
                           gb|AAG04685.1|AE004559_4
                           (AE004559) probable 2-hydroxyacid dehydrogenase [Pseudomonas aeruginosa PAO1]
                           310
                           99.35
                           99.35
                           0
                           1592.0
     

     
     
120645
                           gb|AAN70162.1|AE016655_7
                           (AE016791) D-isomer specific 2-hydroxyacid dehydrogenase family protein [Pseudomonas putida KT2440]
                           310
                           61.93
                           73.22
                           0
                           973.0
     

     
     
120646
                           gb|AAO57386.1|
                           (AE016870) D-isomer specific 2-hydroxyacid dehydrogenase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           310
                           60.32
                           72.58
                           0
                           945.0
     

     






      

          
GC ORFID: 45347How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1296Glimmer Score: 

          
Start: 726311Stop: 727243

          
Length: 933


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1296 translation of PA1296Homolog Bit Score 617.0

          
Other Homologs:  psyr_15may02_Scaffold10_revised_gene1737 (ORF: BLASTX 368.0), t_PA1296 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGCTTGCTGATTCTCGAACGTGACCACGCTCTCTATGCCGCGCTGCTGATGGCGGCCGACCCCAGCCTG 
AAGGTGGTGGCGGGGGACGACCCCCTGCAACTCATCGACGCCGCCAGCGAGTGCTCGATATGGCTCGGCCAG 
CCCGACCTGGTGGCGCAGATGCTGCGCCAGGGCGTGCATCCGGTCTGGGTGCAGTCGACCTGGGCGGGCATC 
ACGCCGCTGCTGGCCGCCGACCTGCCGAAGGACTACAGCCTGACACGCGCGGTAGGCATCTTCGGCCAGGTG 
ATGAGCGAATACCTGCTGACCTACATGCTCGCCCACGAGCGCCAGTTCCTCGGTCGCCTGGCCAGCCAGGTC 
GGTTCGCAATGGGATAGCCGCACGCCTGGCGGGTTGCGTGGCCGGCAGGCGGTGATCGTGGGTACCGGGGAG 
ATCGGCCAGGCGGTGGCGCATACCCTGTCCGGCTTCGGCATGGACCTGACCGGGGTGGCGAAGAATCCGCGT 
TCGCTGGTGCCGTTCAACCGCATGGGCTCGCTCGACGACCTCGGCCGCCTGGTGGAGACGGCCGACTACCTG 
ATCAACCTGCTGCCCGACACCCCCGATACCCACGACATCTACGACCGTGCGCTGTTCGCCCGGCTCAAATCC 
ACGGCGCTGTTCATCAACGCCGGGCGTGGCGTCGCGGTGGTGGACGCCGACCTGGTGGCGGCGCTGGAGAAC 
AACCAGCTGGCCGGCGCGGTGATCGACGTCTGCCGCGAAGAGCCGCTGCCGGCCAACCACCTGTTCTGGCAT 
ACCCCGCGCCTCTTGCTGACCGGCCACACCGCCGCGCCGACCCTGCCGGGGGCGATGGTCGAACTGTTCCGC 
GACAACCTCGCGCGCTTCTGGGCGGGCACCGCCATGCGTGGCGAGGTGGACTTCGCTCGCGGCTACTGA