ViewGene
Locus Name:
GeneID:
Location:

GeneID:2334
TrivialName: PA14_57720
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57720
PAO1 Ortholog Locus:PA4443
Sequence Length:918
Protein Length:305
Start: 5141115
Stop: 5140198
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07831.1|AE004858_9 (AE004858) ATP sulfurylase small subunit [Pseudomonas aeruginosa PAO1]
Identities = 305/305 (100%), Positives = 305/305 (100%)
Sequence:
ATGGTCGACAAACTGACGCACCTGAAACAGCTGGAGGCGGAAAGCATCCACATCATCCGCGAGGTGGCCGCC
GAGTTCGATAACCCGGTGATGCTGTACTCGATCGGCAAGGATTCCGCGGTCATGCTGCACCTGGCCCGCAAG
GCCTTCTTCCCCGGCAAGCTGCCCTTCCCGGTGATGCACGTGGACACCCGCTGGAAATTCCAGGAGATGTAC
AGGTTCCGCGATCGGATGGTCGAGGAAATGGGCCTGGACCTGATCACCCACGTCAACCCGGACGGCGTCGCC
CAGGGCATCAACCCGTTCACCCACGGCAGCGCCAAGCACACCGATGTGATGAAGACCGAGGGACTCAAGCAG
GCCCTGGACAAGTACGGTTTCGACGCTGCCTTCGGCGGTGCGCGCCGCGACGAGGAGAAGTCGCGGGCCAAG
GAACGGGTCTATTCGTTCCGCGACAGCAAGCACCGCTGGGACCCGAAGAACCAGCGTCCCGAGCTGTGGAAC
ATCTACAACGGCAAGGTGAAGAAGGGCGAGTCGATCCGCGTCTTCCCGCTGTCCAACTGGACCGAGCTGGAC
ATCTGGCAATACATCTACCTGGAAGGCATCCCGATCGTCCCGCTGTACTTCGCCGCCGAGCGCGAGGTTATC
GAGAAGAACGGCACGCTGATCATGATCGACGACGAGCGCATCCTCGAGCATCTCTCTGACGAAGAGAAAGCC
CGCATCGAGAAGCGCATGGTGCGCTTCCGTACCCTCGGCTGCTACCCGCTCACCGGCGCGGTCGAGTCCAGC
GCCACCACGCTGCCGGAAATCATCCAGGAAATGCTCCTGACGCGTACTTCCGAACGCCAGGGCCGGGTCATC
GACCATGACCAGGCCGGTTCGATGGAAGAAAAGAAACGTCAGGGCTATTTCTGA
Translation:
MVDKLTHLKQLEAESIHIIREVAAEFDNPVMLYSIGKDSAVMLHLARKAFFPGKLPFPVMHVDTRWKFQEMY
RFRDRMVEEMGLDLITHVNPDGVAQGINPFTHGSAKHTDVMKTEGLKQALDKYGFDAAFGGARRDEEKSRAK
ERVYSFRDSKHRWDPKNQRPELWNIYNGKVKKGESIRVFPLSNWTELDIWQYIYLEGIPIVPLYFAAEREVI
EKNGTLIMIDDERILEHLSDEEKARIEKRMVRFRTLGCYPLTGAVESSATTLPEIIQEMLLTRTSERQGRVI
DHDQAGSMEEKKRQGYF*
AnnotationID:2342GeneID:2334
AnnotatorUID: diggins
Modification Date/Time: 2005-05-02 21:09:09
Gene Name:cysD
Confidence Code:1
GeneProduct: ATP sulfurylase small subunit
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name: sulfate adenylate transferase subunit 2
Functional Category Confidence Code:5
COGs:COG0175,pfam01507
Secondary Functional Category(ies):
EC Number:2.7.7.4
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57882.1| (AE016872) sulfate adenylate transferase, subunit 2 [Pseudomonas
           syringae pv. tomato str. DC3000]
          Length = 305

 Score =  592 bits (1527), Expect = e-169
 Identities = 287/305 (94%), Positives = 298/305 (97%)


>gb|AAN66927.1|AE016321_3 (AE016778) sulfate adenylyltransferase, subunit 2 [Pseudomonas
           putida KT2440]
          Length = 305

 Score =  586 bits (1510), Expect = e-167
 Identities = 284/305 (93%), Positives = 297/305 (97%)






 
Structural Features:
COG0175, CysH, 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase 
(PAPS reductase)/FAD synthetase and related enzymes 
[Amino acid transport and metabolism / Coenzyme metabolism]
CD-Length = 261 residues,  93.9% aligned
Score =  226 bits (577), Expect = 6e-60
Identities = 118/308 (38%), Positives = 144/308 (46%), Gaps = 71/308 (23%)

pfam01507, PAPS_reduct, Phosphoadenosine phosphosulfate 
reductase family.
CD-Length = 197 residues,  91.9% aligned
Score =  203 bits (518), Expect = 5e-53
Identities = 87/199 (43%), Positives = 109/199 (54%), Gaps = 18/199 (9%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4671




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21817




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31482




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2334_cdd.html


    		




 
URL:

















    ReferenceID:32829




  
Author/Investigator(s):
        Leyh TS, Vogt TF, Suo Y.





  
Title:
        The DNA sequence of the sulfate activation locus from Escherichia coli K-12.




  
PubMed:
        1316900


MedLine:
        




    
Source:
J Biol Chem. 1992 May 25;267(15):10405-10.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1316900

















    ReferenceID:32830




  
Author/Investigator(s):
        Tei H, Murata K, Kimura A.





  
Title:
        Molecular cloning of the cys genes (cysC, cysD, cysH, cysI, cysJ, and cysG) responsible for cysteine biosynthesis in Escherichia coli K-12.




  
PubMed:
        2158792


MedLine:
        




    
Source:
Biotechnol Appl Biochem. 1990 Apr;12(2):212-6.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2158792

















    ReferenceID:32831




  
Author/Investigator(s):
        Malo MS, Loughlin RE.





  
Title:
        Promoter elements and regulation of expression of the cysD gene of Escherichia coli K-12.




  
PubMed:
        2185135


MedLine:
        




    
Source:
Gene. 1990 Mar 1;87(1):127-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2185135









           
Homologs By Global Alignment 

           

       



     
Gene ID:2334
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3967
                           PA4443_tr
                           translation of PA4443
                           305
                           99.99
                           99.99
                           0
                           1600.0
     

     
     
120375
                           gb|AAG07831.1|AE004858_9
                           (AE004858) ATP sulfurylase small subunit [Pseudomonas aeruginosa PAO1]
                           305
                           99.99
                           99.99
                           0
                           1600.0
     

     
     
120376
                           gb|AAC46386.1|
                           (AF035608) ATP sulfurylase small subunit [Pseudomonas aeruginosa]
                           305
                           99.99
                           99.99
                           0
                           1600.0
     

     
     
120377
                           gb|AAO57882.1|
                           (AE016872) sulfate adenylate transferase, subunit 2 [Pseudomonas syringae pv. tomato str. DC3000]
                           305
                           94.09
                           97.70
                           0
                           1527.0
     

     
     
120378
                           gb|AAN66927.1|AE016321_3
                           (AE016778) sulfate adenylyltransferase, subunit 2 [Pseudomonas putida KT2440]
                           305
                           93.11
                           97.37
                           0
                           1510.0
     

     






      

          
GC ORFID: 45336How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4443Glimmer Score: 

          
Start: 4404794Stop: 4403877

          
Length: 918


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4443 translation of PA4443Homolog Bit Score 620.0

          
Other Homologs:  psyr_15may02_Scaffold12_revised_gene2023 (ORF: BLASTX 592.0), t_PA4443 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGTCGACAAACTGACGCACCTGAAACAGCTGGAGGCGGAAAGCATCCACATCATCCGCGAGGTGGCCGCC 
GAGTTCGATAACCCGGTGATGCTGTACTCGATCGGCAAGGATTCCGCGGTCATGCTGCACCTGGCCCGCAAG 
GCCTTCTTCCCCGGCAAGCTGCCCTTCCCGGTGATGCACGTGGACACCCGCTGGAAATTCCAGGAGATGTAC 
AGGTTCCGCGATCGGATGGTCGAGGAAATGGGCCTGGACCTGATCACCCACGTCAACCCGGACGGCGTCGCC 
CAGGGCATCAACCCGTTCACCCACGGCAGCGCCAAGCACACCGATGTGATGAAGACCGAGGGACTCAAGCAG 
GCCCTGGACAAGTACGGTTTCGACGCTGCCTTCGGCGGTGCGCGCCGCGACGAGGAGAAGTCGCGGGCCAAG 
GAACGGGTCTATTCGTTCCGCGACAGCAAGCACCGCTGGGACCCGAAGAACCAGCGTCCCGAGCTGTGGAAC 
ATCTACAACGGCAAGGTGAAGAAGGGCGAGTCGATCCGCGTCTTCCCGCTGTCCAACTGGACCGAGCTGGAC 
ATCTGGCAATACATCTACCTGGAAGGCATCCCGATCGTCCCGCTGTACTTCGCCGCCGAGCGCGAGGTTATC 
GAGAAGAACGGCACGCTGATCATGATCGACGACGAGCGCATCCTCGAGCATCTCTCTGACGAAGAGAAAGCC 
CGCATCGAGAAGCGCATGGTGCGCTTCCGTACCCTCGGCTGCTACCCGCTCACCGGCGCGGTCGAGTCCAGC 
GCCACCACGCTGCCGGAAATCATCCAGGAAATGCTCCTGACGCGTACTTCCGAACGCCAGGGCCGGGTCATC 
GACCATGACCAGGCCGGTTCGATGGAAGAAAAGAAACGTCAGGGCTATTTCTGA