ViewGene
Locus Name:
GeneID:
Location:

GeneID:2326
TrivialName: PA14_60470
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_60470
PAO1 Ortholog Locus:PA4569
Sequence Length:969
Protein Length:322
Start: 5389330
Stop: 5390298
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07957.1|AE004870_8 (AE004870) octaprenyl-diphosphate synthase [Pseudomonas aeruginosa PAO1]
Identities = 322/322 (100%), Positives = 322/322 (100%)
Sequence:
ATGCAACCCCAGGCCTTCTACCGCGTGGTGGCGGAAGATTTCACCGCCGTCGACGGGATCATCCGTCGCCAA
CTCACCTCCCGCGTTCCCCTGGTGGAAAAGATCGGCGACTACATCATCTCCGCCGGCGGCAAGCGCCTGCGT
CCGCTGCTGGTCCTGCTCGCCGGCAAGACCCTGGGCTACAAGGGCGACGACCTCTGCCTGCTGGCCGCCACC
ATCGAATTCCTGCACACCTCCACTCTGCTGCACGACGACGTCGTCGACGCCTCCGGCCTGCGCCGCGGCCGC
TCCACCGCCAATGCCCTGTGGGGCAACGCGCCGAGCGTGCTGGTAGGCGACTTCCTTTATGCCCGCTCCTTC
GAAATGATGGTCGAGCTGGGTTCCATGCCGGTCATGCGCATCATATCCCAGGCCACCCGGGTGATCGCCGAG
GGCGAAGTGCTGCAATTGTCCAAGGTACGCGACGCCAGCACTACCGAAGAGACCTACATGGAAGTGATCCGC
GGCAAGACCGCGATGCTTTTCGAGGCCTCGACCCACAGCGCCGCCGCGCTGTGCCAGGCCGGCGAGGAACAG
TCCGAGGCACTGCGCCGCTTCGGCGACTACCTGGGAATCGCCTTCCAACTGGTGGACGACCTGCTCGACTAC
CGCGGCGACGCCGCGACCCTCGGCAAGAACGTCGGCGACGACCTCGCCGAAGGCAAGCCCACCCTGCCGCTG
ATCGTCACCATGCGCGACGGCACCGAGGAGCAGGCCGCGCTGGTACGCAAGGCCATCCAGCAAGGCGGCAGC
CAGGACCTGGAGAGCGTCTGTGCCGCCGTCGAGGCTGCCGGCGCCCTGGACTATACCGCCAACCTCGCCCGC
GACTACGCCGCCCGCGCCATCGCCTGCCTGGATACGCTGCCGGACAACGAATACCGCTCCGCCCTGGTCGAA
CTCAGCGAATTCGCTGTCGCCCGTACCCACTGA
Translation:
MQPQAFYRVVAEDFTAVDGIIRRQLTSRVPLVEKIGDYIISAGGKRLRPLLVLLAGKTLGYKGDDLCLLAAT
IEFLHTSTLLHDDVVDASGLRRGRSTANALWGNAPSVLVGDFLYARSFEMMVELGSMPVMRIISQATRVIAE
GEVLQLSKVRDASTTEETYMEVIRGKTAMLFEASTHSAAALCQAGEEQSEALRRFGDYLGIAFQLVDDLLDY
RGDAATLGKNVGDDLAEGKPTLPLIVTMRDGTEEQAALVRKAIQQGGSQDLESVCAAVEAAGALDYTANLAR
DYAARAIACLDTLPDNEYRSALVELSEFAVARTH*
AnnotationID:2334GeneID:2326
AnnotatorUID: diggins
Modification Date/Time: 2005-05-05 08:08:50
Gene Name:ispB
Confidence Code:2
GeneProduct:octylprenyl diphosphate synthase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0142,pfam00348
Secondary Functional Category(ies):
EC Number:2.5.1.-
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54338.1| (AE016858) octylprenyl diphosphate synthase [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 322

 Score =  534 bits (1376), Expect = e-151
 Identities = 272/322 (84%), Positives = 299/322 (92%)

>gb|AAN82385.1|AE016767_145 (AE016767) Octaprenyl-diphosphate synthase [Escherichia coli
           CFT073]
          Length = 323

 Score =  324 bits (831), Expect = 4e-88
 Identities = 168/321 (52%), Positives = 218/321 (67%), Gaps = 1/321 (0%)






 
Structural Features:
COG0142, IspA, Geranylgeranyl pyrophosphate synthase 
[Coenzyme metabolism]
CD-Length = 322 residues,  99.4% aligned
Score =  289 bits (741), Expect = 6e-79
Identities = 126/324 (38%), Positives = 190/324 (58%), Gaps = 11/324 (3%)

pfam00348, polyprenyl_synt, Polyprenyl synthetase.
CD-Length = 260 residues,  98.1% aligned
Score =  217 bits (555), Expect = 2e-57
Identities = 105/258 (40%), Positives = 148/258 (57%), Gaps = 9/258 (3%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4655




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21801




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31644




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2326_cdd.html


    		




 
URL:

















    ReferenceID:33205




  
Author/Investigator(s):
        Okada K, Minehira M, Zhu X, Suzuki K, Nakagawa T, Matsuda H, Kawamukai M.





  
Title:
        The ispB gene encoding octaprenyl diphosphate synthase is essential for growth of Escherichia coli.




  
PubMed:
        9139929


MedLine:
        




    
Source:
J Bacteriol. 1997 May;179(9):3058-60.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9139929

















    ReferenceID:33206




  
Author/Investigator(s):
        Asai K, Fujisaki S, Nishimura Y, Nishino T, Okada K, Nakagawa T, Kawamukai M, Matsuda H.





  
Title:
        The identification of Escherichia coli ispB (cel) gene encoding the octaprenyl diphosphate synthase.




  
PubMed:
        8037730


MedLine:
        




    
Source:
Biochem Biophys Res Commun. 1994 Jul 15;202(1):340-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8037730









           
Homologs By Global Alignment 

           

       



     
Gene ID:2326
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3956
                           PA4569_tr
                           translation of PA4569
                           322
                           99.99
                           99.99
                           0
                           1603.0
     

     
     
120221
                           gb|AAG07957.1|AE004870_8
                           (AE004870) octaprenyl-diphosphate synthase [Pseudomonas aeruginosa PAO1]
                           322
                           99.99
                           99.99
                           0
                           1603.0
     

     
     
120222
                           gb|AAO54338.1|
                           (AE016858) octylprenyl diphosphate synthase [Pseudomonas syringae pv. tomato str. DC3000]
                           322
                           84.47
                           92.85
                           0
                           1376.0
     

     
     
120223
                           gb|AAD47627.1|
                           (AF153713) octylprenyl diphosphate synthase-like protein [Pseudomonas sp. BG33R]
                           322
                           83.54
                           91.92
                           0
                           1364.0
     

     
     
120224
                           gb|AAN66312.1|AE016259_10
                           (AE016776) octylprenyl diphosphate synthase [Pseudomonas putida KT2440]
                           322
                           82.91
                           90.68
                           0
                           1342.0
     

     






      

          
GC ORFID: 45328How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4569Glimmer Score: 

          
Start: 4653010Stop: 4653978

          
Length: 969


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4569 translation of PA4569Homolog Bit Score 622.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3569 (ORF: BLASTX 535.0), t_PA4569 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCAACCCCAGGCCTTCTACCGCGTGGTGGCGGAAGATTTCACCGCCGTCGACGGGATCATCCGTCGCCAA 
CTCACCTCCCGCGTTCCCCTGGTGGAAAAGATCGGCGACTACATCATCTCCGCCGGCGGCAAGCGCCTGCGT 
CCGCTGCTGGTCCTGCTCGCCGGCAAGACCCTGGGCTACAAGGGCGACGACCTCTGCCTGCTGGCCGCCACC 
ATCGAATTCCTGCACACCTCCACTCTGCTGCACGACGACGTCGTCGACGCCTCCGGCCTGCGCCGCGGCCGC 
TCCACCGCCAATGCCCTGTGGGGCAACGCGCCGAGCGTGCTGGTAGGCGACTTCCTTTATGCCCGCTCCTTC 
GAAATGATGGTCGAGCTGGGTTCCATGCCGGTCATGCGCATCATATCCCAGGCCACCCGGGTGATCGCCGAG 
GGCGAAGTGCTGCAATTGTCCAAGGTACGCGACGCCAGCACTACCGAAGAGACCTACATGGAAGTGATCCGC 
GGCAAGACCGCGATGCTTTTCGAGGCCTCGACCCACAGCGCCGCCGCGCTGTGCCAGGCCGGCGAGGAACAG 
TCCGAGGCACTGCGCCGCTTCGGCGACTACCTGGGAATCGCCTTCCAACTGGTGGACGACCTGCTCGACTAC 
CGCGGCGACGCCGCGACCCTCGGCAAGAACGTCGGCGACGACCTCGCCGAAGGCAAGCCCACCCTGCCGCTG 
ATCGTCACCATGCGCGACGGCACCGAGGAGCAGGCCGCGCTGGTACGCAAGGCCATCCAGCAAGGCGGCAGC 
CAGGACCTGGAGAGCGTCTGTGCCGCCGTCGAGGCTGCCGGCGCCCTGGACTATACCGCCAACCTCGCCCGC 
GACTACGCCGCCCGCGCCATCGCCTGCCTGGATACGCTGCCGGACAACGAATACCGCTCCGCCCTGGTCGAA 
CTCAGCGAATTCGCTGTCGCCCGTACCCACTGA