ViewGene
Locus Name:
GeneID:
Location:

GeneID:2275
TrivialName: PA14_41900
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_41900
PAO1 Ortholog Locus:PA1752
Sequence Length:948
Protein Length:315
Start: 3742163
Stop: 3741216
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG05141.1|AE004601_6 (AE004601) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 314/315 (99%), Positives = 314/315 (99%)
Sequence:
ATGGATTCAAAGCAGCAACGGATCGGCGTCATCGGTACGGGAGCCATCGGCGGCTTCTATGGATTGATGCTG
GCGCATGCCGGTCACGACGTGCATTTTCTCCTGCGCAGCGAGTTCGAGGCGGTCAATCGCGCCGGACTCAGC
CTGAATAGTGCGGTGCATGGCTTCCGGCGCCTGGCGCCGGTCCAGGCCTACCATTCGGCGCAGGACATGCCG
CCCTGCGACTGGTTGCTGGTGGGCGCCAAGACCACCGGCAATCACGAACTGGCACCCCTGATCCGCGCAGCG
GCGGCGCCGGGTGCCAAGGTTCTGCTGCTGCAGAACGGTCTGGGCGTGGAGGAGCGGCTGCGGCCGTTGCTG
CCGGAAAGCCTGCATCTGCTGGGCGGCCTCTGCTTCATCTGTGTGCATCGCGGCGAACCGGGCGTGATCGAG
CACCAGGCCTACGGCGGAGTGAATCTCGGCTATCATTCCGGACCGGCCGACGAGCGACGCCGGCGCGAGATC
GTCGAGGAGGGTGCCGCGCTGTTCCGCGAGTCCGGGCTGGAGTCCACGGCGATGCCCGACCTGGAGCAGGCG
CGCTGGCAGAAGCTGGTCTGGAACATCCCCTACAACGGGCTGTCGGTATTGCTGAAGAGCAGCACCGCGCCG
CTGATGGCCAACGCGGACAGTCGGTCGCTGATCGAGGCGATCATGGAGGAAGTCATCGGTGCTGCCGGCGCC
TGCGGCTTCATCCTGCCTGAGGGCTATGCGGACCAACTGTTGGCGGCGACCGAGCGGATGCCCGACTATCGG
CCGAGCATGTACCACGACTTCGCCCATGGGCGGCCACTGGAGCTGGCGGCGATCTATGCCGCGCCCCTGGCC
CGCGCTGCTGCTGCGGGCTACCGGATGCCCAGGGTCGAGGCGCTGCACCAGGCGTTGCGCTTCCTCGAAGCG
CAACCGCGCTGA
Translation:
MDSKQQRIGVIGTGAIGGFYGLMLAHAGHDVHFLLRSEFEAVNRAGLSLNSAVHGFRRLAPVQAYHSAQDMP
PCDWLLVGAKTTGNHELAPLIRAAAAPGAKVLLLQNGLGVEERLRPLLPESLHLLGGLCFICVHRGEPGVIE
HQAYGGVNLGYHSGPADERRRREIVEEGAALFRESGLESTAMPDLEQARWQKLVWNIPYNGLSVLLKSSTAP
LMANADSRSLIEAIMEEVIGAAGACGFILPEGYADQLLAATERMPDYRPSMYHDFAHGRPLELAAIYAAPLA
RAAAAGYRMPRVEALHQALRFLEAQPR*
AnnotationID:2283GeneID:2275
AnnotatorUID: diggins
Modification Date/Time: 2005-05-11 06:06:35
Gene Name:panE
Confidence Code:2
GeneProduct:ketopantoate reductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:2-dehydropantoate 2-reductase
Functional Category:(8) Central intermediary metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1893
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67938.1|AE016426_3 (AE016782) ketopantoate reductase [Pseudomonas putida KT2440]
          Length = 315

 Score =  420 bits (1079), Expect = e-117
 Identities = 213/315 (67%), Positives = 246/315 (78%), Gaps = 2/315 (0%)

>gb|AAO55790.1| (AE016864) 2-dehydropantoate 2-reductase [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 323

 Score =  402 bits (1034), Expect = e-111
 Identities = 198/306 (64%), Positives = 237/306 (77%), Gaps = 1/306 (0%)







 
Structural Features:
COG1893, ApbA, Ketopantoate reductase [Coenzyme metabolism]
CD-Length = 307 residues,  99.0% aligned
Score =  250 bits (641), Expect = 1e-67
Identities = 112/312 (35%), Positives = 154/312 (49%), Gaps = 10/312 (3%)








   
Genomic Context:






   
Comment:


















    ReferenceID:4553




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21699




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37171




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2275_cdd.html


    		




 
URL:

















    ReferenceID:38130




  
Author/Investigator(s):
        Elischewski F, Puhler A, Kalinowski J.





  
Title:
        Pantothenate production in Escherichia coli K12 by enhanced expression of the panE gene encoding ketopantoate reductase.




  
PubMed:
        10553653


MedLine:
        




    
Source:
J Biotechnol. 1999 Oct 8;75(2-3):135-46.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10553653

















    ReferenceID:38131




  
Author/Investigator(s):
        Frodyma ME, Downs D.





  
Title:
        The panE gene, encoding ketopantoate reductase, maps at 10 minutes and is allelic to apbA in Salmonella typhimurium.




  
PubMed:
        9721324


MedLine:
        




    
Source:
J Bacteriol. 1998 Sep;180(17):4757-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9721324

















    ReferenceID:38132




  
Author/Investigator(s):
        Matak-Vinkovic D, Vinkovic M, Saldanha SA, Ashurst JL, von Delft F, Inoue T, Miguel RN, Smith AG, Blundell TL, Abell C.





  
Title:
        Crystal structure of Escherichia coli ketopantoate reductase at 1.7 A resolution and insight into the enzyme mechanism.




  
PubMed:
        11724562


MedLine:
        




    
Source:
Biochemistry. 2001 Dec 4;40(48):14493-500.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11724562









           
Homologs By Global Alignment 

           

       



     
Gene ID:2275
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3869
                           PA1752_tr
                           translation of PA1752
                           315
                           99.68
                           99.68
                           0
                           1625.0
     

     
     
118396
                           gb|AAG05141.1|AE004601_6
                           (AE004601) hypothetical protein PA1752 [Pseudomonas aeruginosa PAO1]
                           315
                           99.68
                           99.68
                           0
                           1625.0
     

     
     
118397
                           gb|AAN67938.1|AE016426_3
                           (AE016782) ketopantoate reductase [Pseudomonas putida KT2440]
                           317
                           67.19
                           77.60
                           4
                           1083.0
     

     
     
118398
                           gb|AAO55790.1|
                           (AE016864) 2-dehydropantoate 2-reductase [Pseudomonas syringae pv. tomato str. DC3000]
                           323
                           61.60
                           74.30
                           8
                           1035.0
     

     
     
118400
                           gb|AAR36055.1|
                           (AE017216) 2-dehydropantoate 2-reductase [Geobacter sulfurreducens PCA]
                           318
                           43.39
                           56.28
                           19
                           596.0
     

     






      

          
GC ORFID: 45277How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1752Glimmer Score: 

          
Start: 1211746Stop: 1212693

          
Length: 948


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1752 translation of PA1752Homolog Bit Score 630.0

          
Other Homologs:  t_PA1752 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGATTCAAAGCAGCAACGGATCGGCGTCATCGGTACGGGAGCCATCGGCGGCTTCTATGGATTGATGCTG 
GCGCATGCCGGTCACGACGTGCATTTTCTCCTGCGCAGCGAGTTCGAGGCGGTCAATCGCGCCGGACTCAGC 
CTGAATAGTGCGGTGCATGGCTTCCGGCGCCTGGCGCCGGTCCAGGCCTACCATTCGGCGCAGGACATGCCG 
CCCTGCGACTGGTTGCTGGTGGGCGCCAAGACCACCGGCAATCACGAACTGGCACCCCTGATCCGCGCAGCG 
GCGGCGCCGGGTGCCAAGGTTCTGCTGCTGCAGAACGGTCTGGGCGTGGAGGAGCGGCTGCGGCCGTTGCTG 
CCGGAAAGCCTGCATCTGCTGGGCGGCCTCTGCTTCATCTGTGTGCATCGCGGCGAACCGGGCGTGATCGAG 
CACCAGGCCTACGGCGGAGTGAATCTCGGCTATCATTCCGGACCGGCCGACGAGCGACGCCGGCGCGAGATC 
GTCGAGGAGGGTGCCGCGCTGTTCCGCGAGTCCGGGCTGGAGTCCACGGCGATGCCCGACCTGGAGCAGGCG 
CGCTGGCAGAAGCTGGTCTGGAACATCCCCTACAACGGGCTGTCGGTATTGCTGAAGAGCAGCACCGCGCCG 
CTGATGGCCAACGCGGACAGTCGGTCGCTGATCGAGGCGATCATGGAGGAAGTCATCGGTGCTGCCGGCGCC 
TGCGGCTTCATCCTGCCTGAGGGCTATGCGGACCAACTGTTGGCGGCGACCGAGCGGATGCCCGACTATCGG 
CCGAGCATGTACCACGACTTCGCCCATGGGCGGCCACTGGAGCTGGCGGCGATCTATGCCGCGCCCCTGGCC 
CGCGCTGCTGCTGCGGGCTACCGGATGCCCAGGGTCGAGGCGCTGCACCAGGCGTTGCGCTTCCTCGAAGCG 
CAACCGCGCTGA