ViewGene
Locus Name:
GeneID:
Location:

GeneID:2266
TrivialName: PA14_64810
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_64810
PAO1 Ortholog Locus:PA4905
Sequence Length:954
Protein Length:317
Start: 5775527
Stop: 5776480
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08290.1|AE004903_8 (AE004903) vanillate O-demethylase oxidoreductase [Pseudomonas aeruginosa PAO1]
Identities = 309/317 (97%), Positives = 312/317 (98%)
Sequence:
ATGATCGAGGTCATCGTCGGCGCCATCCGCCTGGAGGCGCAGGATATCCACAGCTTCGAACTGTTCCGGGCC
GACGGGGCGGCCCTGCCGTCCTTCGAGCCGGGCGCACACATCGACCTGCACCTGCCCAACGGCCTGGTCCGC
CAGTACTCCCTCTGCGGCCCCGCCGAACGGCCGCGCCACTACCGTATCGCCGTACTCCGCTGCCGCGACTCG
CGCGGCGGCTCGGCCACGCTGCACGCCGAACTGCGGGTCGGCCAGCGGCTGCGCATCGGCGAGCCGCGCAAT
CTCTTCCCGCTGTCGCCGGAGCCCGGTCCGCGCCTGCTGTTCGCCGGCGGCATCGGCATCACGCCGCTGCTG
GCGATGGCCGAACGCCTGGCCCGCGATGGCGCCAACTTCCAGTTGCACTACTGCGCGCATTCCGCCGAGCGC
GCGGCCTTCGTCGACTATCTTGACCGGTGCGCCTTCGCCGACCGCGTGCACTGCCACTTCGACCACGGCGAC
AGCAGCCGCCGCGCGGACCTTCGCGCCCTGCTGGCGGCCAGTCCACGCGACGCCCAGCTATACCTTTGCGGG
CCGGCGGGCTTCATGCAGTGGATCGAAGAAAGCGCCCGCGAACTGGGCTGGGAAGCAAGCCGCTTGCATCGC
GAGCACTTCGCCGCCGCGCCACGGGACGCCAATGCGGACGGCACCTTCGAAGTGCAGTTGGCGAGCAACGGG
GCGCTGATCCGGGTCGCCGCCGGGCAGACCGTGCTGGCGGCCCTGCGCGAAGCCGGGGTGGACCTGCCGGCC
TCCTGCGAGCAGGGCATCTGCGGCACCTGTCTGACCCGCGTGCTGGACGGCGAGCCGGAGCATCGCGACCTC
TACCTCAGCGAAGAGGAGCAGGCCGCCAACGATTGCTTCACGCCCTGCTGTTCGCGTTCGCGCAGCCCACGG
CTGGTGCTGGATCTCTAG
Translation:
MIEVIVGAIRLEAQDIHSFELFRADGAALPSFEPGAHIDLHLPNGLVRQYSLCGPAERPRHYRIAVLRCRDS
RGGSATLHAELRVGQRLRIGEPRNLFPLSPEPGPRLLFAGGIGITPLLAMAERLARDGANFQLHYCAHSAER
AAFVDYLDRCAFADRVHCHFDHGDSSRRADLRALLAASPRDAQLYLCGPAGFMQWIEESARELGWEASRLHR
EHFAAAPRDANADGTFEVQLASNGALIRVAAGQTVLAALREAGVDLPASCEQGICGTCLTRVLDGEPEHRDL
YLSEEEQAANDCFTPCCSRSRSPRLVLDL*
AnnotationID:2274GeneID:2266
AnnotatorUID: diggins
Modification Date/Time: 2005-04-26 07:07:34
Gene Name:vanB
Confidence Code:2
GeneProduct:vanillate O-demethylase oxidoreductase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(5) Carbon compound catabolism
Alternate Gene Product Name:putative flavodoxin reductase
Functional Category Confidence Code:5
COGs:COG1018
Secondary Functional Category(ies):energy metabolism
EC Number:1.14.13.-
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAB93957.1| (AJ245887) reductase-subunit oxygenase [Pseudomonas fluorescens]
          Length = 317

 Score =  368 bits (944), Expect = e-101
 Identities = 186/317 (58%), Positives = 235/317 (74%), Gaps = 1/317 (0%)

>emb|CAA72288.1| (Y11521) reductase-subunit oxygenase [Pseudomonas sp.]
          Length = 317

 Score =  360 bits (925), Expect = 5e-99
 Identities = 179/317 (56%), Positives = 224/317 (70%)

>gb|AAN69333.1|AE016570_10 (AE016788) vanillate O-demethylase oxidoreductase [Pseudomonas
           putida KT2440]
          Length = 316

 Score =  356 bits (913), Expect = 1e-97
 Identities = 179/317 (56%), Positives = 223/317 (70%), Gaps = 1/317 (0%)








 
Structural Features:
COG1018, Hmp, Flavodoxin reductases (ferredoxin-NADPH reductases) family 1 
[Energy production and conversion]
CD-Length = 266 residues,  98.5% aligned
Score =  214 bits (545), Expect = 2e-56
Identities = 96/265 (36%), Positives = 125/265 (47%), Gaps = 12/265 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4535




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21681




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:35155




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2266_cdd.html


    		




 
URL:

















    ReferenceID:35930




  
Author/Investigator(s):
        Segura A, Bunz PV, D'Argenio DA, Ornston LN.





  
Title:
        Genetic analysis of a chromosomal region containing vanA and vanB, genes required for conversion of either ferulate or vanillate to protocatechuate in Acinetobacter.




  
PubMed:
        10348863


MedLine:
        




    
Source:
J Bacteriol. 1999 Jun;181(11):3494-504.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10348863

















    ReferenceID:35933




  
Author/Investigator(s):
        Brunel F, Davison J.





  
Title:
        Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase.




  
PubMed:
        3170489


MedLine:
        




    
Source:
J Bacteriol. 1988 Oct;170(10):4924-30.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=3170489









           
Homologs By Global Alignment 

           

       



     
Gene ID:2266
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3854
                           PA4905_tr
                           translation of PA4905
                           317
                           97.47
                           98.42
                           0
                           1630.0
     

     
     
118282
                           gb|AAG08290.1|AE004903_8
                           (AE004903) vanillate O-demethylase oxidoreductase [Pseudomonas aeruginosa PAO1]
                           317
                           97.47
                           98.42
                           0
                           1630.0
     

     
     
118283
                           emb|CAB93957.1|
                           (AJ245887) reductase-subunit oxygenase [Pseudomonas fluorescens]
                           318
                           58.49
                           73.89
                           2
                           946.0
     

     
     
118284
                           emb|CAA72288.1|
                           (Y11521) reductase-subunit oxygenase [Pseudomonas sp.]
                           317
                           56.46
                           70.66
                           0
                           925.0
     

     
     
118285
                           gb|AAN69333.1|AE016570_10
                           (AE016788) vanillate O-demethylase oxidoreductase [Pseudomonas putida KT2440]
                           317
                           56.46
                           70.34
                           1
                           915.0
     

     






      

          
GC ORFID: 45268How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4905Glimmer Score: 

          
Start: 5039208Stop: 5040161

          
Length: 954


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA4905 translation of PA4905Homolog Bit Score 632.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene311 (ORF: BLASTX 355.0), t_PA3498 (ORF: BLASTX 246.0), t_PA4905 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGATCGAGGTCATCGTCGGCGCCATCCGCCTGGAGGCGCAGGATATCCACAGCTTCGAACTGTTCCGGGCC 
GACGGGGCGGCCCTGCCGTCCTTCGAGCCGGGCGCACACATCGACCTGCACCTGCCCAACGGCCTGGTCCGC 
CAGTACTCCCTCTGCGGCCCCGCCGAACGGCCGCGCCACTACCGTATCGCCGTACTCCGCTGCCGCGACTCG 
CGCGGCGGCTCGGCCACGCTGCACGCCGAACTGCGGGTCGGCCAGCGGCTGCGCATCGGCGAGCCGCGCAAT 
CTCTTCCCGCTGTCGCCGGAGCCCGGTCCGCGCCTGCTGTTCGCCGGCGGCATCGGCATCACGCCGCTGCTG 
GCGATGGCCGAACGCCTGGCCCGCGATGGCGCCAACTTCCAGTTGCACTACTGCGCGCATTCCGCCGAGCGC 
GCGGCCTTCGTCGACTATCTTGACCGGTGCGCCTTCGCCGACCGCGTGCACTGCCACTTCGACCACGGCGAC 
AGCAGCCGCCGCGCGGACCTTCGCGCCCTGCTGGCGGCCAGTCCACGCGACGCCCAGCTATACCTTTGCGGG 
CCGGCGGGCTTCATGCAGTGGATCGAAGAAAGCGCCCGCGAACTGGGCTGGGAAGCAAGCCGCTTGCATCGC 
GAGCACTTCGCCGCCGCGCCACGGGACGCCAATGCGGACGGCACCTTCGAAGTGCAGTTGGCGAGCAACGGG 
GCGCTGATCCGGGTCGCCGCCGGGCAGACCGTGCTGGCGGCCCTGCGCGAAGCCGGGGTGGACCTGCCGGCC 
TCCTGCGAGCAGGGCATCTGCGGCACCTGTCTGACCCGCGTGCTGGACGGCGAGCCGGAGCATCGCGACCTC 
TACCTCAGCGAAGAGGAGCAGGCCGCCAACGATTGCTTCACGCCCTGCTGTTCGCGTTCGCGCAGCCCACGG 
CTGGTGCTGGATCTCTAG