ViewGene
Locus Name:
GeneID:
Location:

GeneID:2261
TrivialName: PA14_30280
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_30280
PAO1 Ortholog Locus:PA2616
Sequence Length:951
Protein Length:316
Start: 2622443
Stop: 2621493
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06004.1|AE004690_4 (AE004690) thioredoxin reductase 1 [Pseudomonas aeruginosa PAO1]
Identities = 315/316 (99%), Positives = 315/316 (99%)
Sequence:
ATGAGTGAAGTCAAGCATTCGCGCCTCATCATCCTGGGCTCCGGTCCGGCCGGCTATACCGCCGCCGTGTAC
GCCGCACGCGCCAATCTCAAGCCAGTGGTGATCACCGGCATCCAGCCAGGCGGCCAGCTGACCACCACCACC
GAAGTCGACAACTGGCCGGGCGACGTCGAAGGCCTTACCGGCCCGGCGCTGATGACCCGCATGCAGCAGCAC
GCCGAGCGTTTCGACACCGAGATCGTCTACGACCACATCCACACCGCCGAGTTGCAACAGCGCCCCTTCACA
CTGAAGGGCGATAGTGGCACTTATACCTGCGATGCATTGATCATTGCCACCGGTGCCTCCGCGCAATACCTG
GGCATGTCGTCGGAAGAAGCCTTCATGGGCAAGGGCGTTTCCGCCTGCGCCACCTGCGACGGCTTCTTCTAC
CGCAACCAGGTGGTCTGCGTGGTCGGCGGCGGCAATACCGCCGTCGAGGAAGCGCTGTACCTGGCCAACATC
GCCAAGGAAGTCCACCTGATCCACCGTCGCGACAAGCTGCGTTCGGAGAAAATCCTCCAGGACAAGCTGTTC
GACAAGGCCGAGAACGGCAACGTGCGCCTGCACTGGAACACCACCCTGGACGAGGTGCTGGGCGACGCCAGC
GGCGTCACCGGCGTACGCCTGAAGAGCACCATCGACGGCAGCACCAGCGAACTGTCGCTGGCCGGCGTGTTC
ATCGCCATCGGCCACAAGCCGAACACCGACCTGTTCCAGGGCCAACTGGAAATGCGCGACGGCTACCTGCGG
ATCCACGGCGGCAGCGAAGGCAACGCCACCCAGACCAGCATCGAAGGCGTGTTCGCCGCCGGCGACGTGGCC
GACCACGTCTACCGCCAGGCCATCACTTCCGCCGGCGCCGGCTGCATGGCCGCGCTGGATGCCGAAAAATAC
CTCGACGACCATTGA
Translation:
MSEVKHSRLIILGSGPAGYTAAVYAARANLKPVVITGIQPGGQLTTTTEVDNWPGDVEGLTGPALMTRMQQH
AERFDTEIVYDHIHTAELQQRPFTLKGDSGTYTCDALIIATGASAQYLGMSSEEAFMGKGVSACATCDGFFY
RNQVVCVVGGGNTAVEEALYLANIAKEVHLIHRRDKLRSEKILQDKLFDKAENGNVRLHWNTTLDEVLGDAS
GVTGVRLKSTIDGSTSELSLAGVFIAIGHKPNTDLFQGQLEMRDGYLRIHGGSEGNATQTSIEGVFAAGDVA
DHVYRQAITSAGAGCMAALDAEKYLDDH*
AnnotationID:2269GeneID:2261
AnnotatorUID: diggins
Modification Date/Time: 2005-04-29 11:11:42
Gene Name:trxB1
Confidence Code:2
GeneProduct:thioredoxin reductase 1
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(18) Nucleotide biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0492,pfam00070
Secondary Functional Category(ies):
EC Number:1.6.4.5
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN55343.1|AE015671_10 (AE015671) thioredoxin reductase [Shewanella oneidensis MR-1]
          Length = 317

 Score =  495 bits (1275), Expect = e-139
 Identities = 238/314 (75%), Positives = 276/314 (87%)

>dbj|BAC59514.1| (AP005077) thioredoxin reductase [Vibrio parahaemolyticus]
          Length = 319

 Score =  493 bits (1270), Expect = e-139
 Identities = 238/314 (75%), Positives = 272/314 (86%)

>gb|AAM86353.1|AE013883_7 (AE013883) thioredoxin reductase [Yersinia pestis KIM]
          Length = 320

 Score =  488 bits (1255), Expect = e-137
 Identities = 239/314 (76%), Positives = 268/314 (85%)








 
Structural Features:
COG0492, TrxB, Thioredoxin reductase 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 305 residues,  99.0% aligned
Score =  344 bits (883), Expect = 1e-95
Identities = 160/314 (50%), Positives = 214/314 (68%), Gaps = 14/314 (4%)

pfam00070, Pyr_redox, Pyridine nucleotide-disulphide oxidoreductase. 
CD-Length = 288 residues, 100.0% aligned
Score =  179 bits (454), Expect = 6e-46
Identities = 88/298 (29%), Positives = 136/298 (45%), Gaps = 18/298 (6%)








   
Genomic Context:






   
Comment:


















    ReferenceID:4525




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21671




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33180




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2261_cdd.html


    		




 
URL:

















    ReferenceID:34551




  
Author/Investigator(s):
        Lubbers M, Andreesen JR.





  
Title:
        Components of glycine reductase from Eubacterium acidaminophilum. Cloning, sequencing and identification of the genes for thioredoxin reductase, thioredoxin and selenoprotein PA.




  
PubMed:
        8223622


MedLine:
        




    
Source:
Eur J Biochem. 1993 Oct 15;217(2):791-8.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8223622

















    ReferenceID:34552




  
Author/Investigator(s):
        Kreimer S, Sohling B, Andreesen JR.





  
Title:
        Two closely linked genes encoding thioredoxin and thioredoxin reductase in Clostridium litorale.




  
PubMed:
        9297471


MedLine:
        




    
Source:
Arch Microbiol. 1997 Oct;168(4):328-37.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9297471









           
Homologs By Global Alignment 

           

       



     
Gene ID:2261
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3847
                           PA2616_tr
                           translation of PA2616
                           316
                           99.68
                           99.68
                           0
                           1631.0
     

     
     
117947
                           gb|AAG06004.1|AE004690_4
                           (AE004690) thioredoxin reductase 1 [Pseudomonas aeruginosa PAO1]
                           316
                           99.68
                           99.68
                           0
                           1631.0
     

     
     
117950
                           gb|AAF94341.1|
                           (AE004198) thioredoxin reductase [Vibrio cholerae O1 biovar eltor str. N16961]
                           318
                           75.15
                           84.90
                           2
                           1264.0
     

     
     
117948
                           gb|AAN55343.1|AE015671_10
                           (AE015671) thioredoxin reductase [Shewanella oneidensis MR-1]
                           317
                           75.07
                           87.06
                           1
                           1272.0
     

     
     
117951
                           gb|AAM86353.1|AE013883_7
                           (AE013883) thioredoxin reductase [Yersinia pestis KIM]
                           320
                           74.68
                           83.75
                           4
                           1251.0
     

     






      

          
GC ORFID: 45263How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2616Glimmer Score: 

          
Start: 2331483Stop: 2332433

          
Length: 951


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2616 translation of PA2616Homolog Bit Score 632.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene4346 (ORF: BLASTX 482.0), t_PA0849 (ORF: BLASTX 474.0), t_PA2616 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGTGAAGTCAAGCATTCGCGCCTCATCATCCTGGGCTCCGGTCCGGCCGGCTATACCGCCGCCGTGTAC 
GCCGCACGCGCCAATCTCAAGCCAGTGGTGATCACCGGCATCCAGCCAGGCGGCCAGCTGACCACCACCACC 
GAAGTCGACAACTGGCCGGGCGACGTCGAAGGCCTTACCGGCCCGGCGCTGATGACCCGCATGCAGCAGCAC 
GCCGAGCGTTTCGACACCGAGATCGTCTACGACCACATCCACACCGCCGAGTTGCAACAGCGCCCCTTCACA 
CTGAAGGGCGATAGTGGCACTTATACCTGCGATGCATTGATCATTGCCACCGGTGCCTCCGCGCAATACCTG 
GGCATGTCGTCGGAAGAAGCCTTCATGGGCAAGGGCGTTTCCGCCTGCGCCACCTGCGACGGCTTCTTCTAC 
CGCAACCAGGTGGTCTGCGTGGTCGGCGGCGGCAATACCGCCGTCGAGGAAGCGCTGTACCTGGCCAACATC 
GCCAAGGAAGTCCACCTGATCCACCGTCGCGACAAGCTGCGTTCGGAGAAAATCCTCCAGGACAAGCTGTTC 
GACAAGGCCGAGAACGGCAACGTGCGCCTGCACTGGAACACCACCCTGGACGAGGTGCTGGGCGACGCCAGC 
GGCGTCACCGGCGTACGCCTGAAGAGCACCATCGACGGCAGCACCAGCGAACTGTCGCTGGCCGGCGTGTTC 
ATCGCCATCGGCCACAAGCCGAACACCGACCTGTTCCAGGGCCAACTGGAAATGCGCGACGGCTACCTGCGG 
ATCCACGGCGGCAGCGAAGGCAACGCCACCCAGACCAGCATCGAAGGCGTGTTCGCCGCCGGCGACGTGGCC 
GACCACGTCTACCGCCAGGCCATCACTTCCGCCGGCGCCGGCTGCATGGCCGCGCTGGATGCCGAAAAATAC 
CTCGACGACCATTGA