ViewGene
Locus Name:
GeneID:
Location:

GeneID:2256
TrivialName: PA14_05310
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_05310
PAO1 Ortholog Locus:PA0407
Sequence Length:954
Protein Length:317
Start: 464122
Stop: 463169
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03796.1|AE004478_1 (AE004478) glutathione synthetase [Pseudomonas aeruginosa PAO1]
Identities = 317/317 (100%), Positives = 317/317 (100%)
Sequence:
ATGAGCGTACGCCTCGGGATCGTCATGGACCCCATCGCGCGCATCAACTTCAAGAAGGACAGCTCGCTCGCC
ATGCTGCTGGCTGCCCAGGCCCGCGGCTGGTCGCTGTTCTACATGGAACAGCAGGACCTCTACCAGAAGGCC
GGCGTCGCGCGTGGACGCATGCGCCCGCTGAAGGTCTTCAACGATGCCTCCCGCTGGTTCGAACTGGAAGCG
GAAAGCGACCAACCGCTGCACGAACTGGACGTGATCCTGATGCGCAAGGACCCGCCCTTCGATAACGAATTC
GTCTATTCGACCTACCTGCTGGAACAGGCCGAGCGTGCCGGCGCCCTGGTGGTGAACCGGCCGCAGAGCCTG
CGCGACTGCAACGAGAAGTTCTTCGCCACGCAGTTCACCCAGTGCACTCCGCCGACGATGGTCAGCCGGCGA
TCCGATATTCTGCGCGAGTTTGCCGCCGAGCATCGTGACATCATTCTCAAACCCCTGGACGGAATGGGCGGT
TCGTCGATATTTCGTCACCGCGAAGGCGACCCGAACCTCTCGGTGATCCTCGAGACCCTCACCCAGCATGGC
AGCCAGCAGATCATGGCGCAGCGCTATCTGCCGGAAATCAAGGACGGCGACAAACGCATCCTGATGATCGAC
GGCGAACCGGTGCCCTACTGCCTGGCGCGCATCCCCGCCCAGGGCGAGACCCGCGGCAACCTCGCCGCCGGC
GGCCGCGGCGTGGCCCAGCCGCTGAGCGAGCGCGACCGCTGGATCGCCGCCGAGGTCGGCCCGCACCTGCGC
GAGCGCGGCCTGCTGTTCGTCGGCCTCGACGTGATCGGCGATTATCTGACGGAAATCAACGTCACCAGCCCG
ACCTGCATCCGCGAGATCGACCAGGCCTTCGACACCCGCATCGGCGACAAGCTGATGGACGCCATCGCCGCC
CAGCTGGCTGCCCGCTGA
Translation:
MSVRLGIVMDPIARINFKKDSSLAMLLAAQARGWSLFYMEQQDLYQKAGVARGRMRPLKVFNDASRWFELEA
ESDQPLHELDVILMRKDPPFDNEFVYSTYLLEQAERAGALVVNRPQSLRDCNEKFFATQFTQCTPPTMVSRR
SDILREFAAEHRDIILKPLDGMGGSSIFRHREGDPNLSVILETLTQHGSQQIMAQRYLPEIKDGDKRILMID
GEPVPYCLARIPAQGETRGNLAAGGRGVAQPLSERDRWIAAEVGPHLRERGLLFVGLDVIGDYLTEINVTSP
TCIREIDQAFDTRIGDKLMDAIAAQLAAR*
AnnotationID:2264GeneID:2256
AnnotatorUID: diggins
Modification Date/Time: 2005-03-25 08:08:31
Gene Name:gshB
Confidence Code:2
GeneProduct:glutathione synthetase"
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(4) Biosynthesis of cofactors, prosthetic groups and carriers
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0189,pfam02955,pfam02951
Secondary Functional Category(ies):amino acid biosynthesis and metabolims
EC Number:6.3.2.3
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70559.1|AE016698_7 (AE016792) glutathione synthetase [Pseudomonas putida KT2440]
          Length = 317

 Score =  521 bits (1342), Expect = e-147
 Identities = 263/317 (82%), Positives = 279/317 (88%)

>gb|AAO58463.1| (AE016874) glutathione synthetase [Pseudomonas syringae pv. tomato
           str. DC3000]
          Length = 319

 Score =  513 bits (1322), Expect = e-145
 Identities = 253/317 (79%), Positives = 280/317 (88%)

>gb|AAM86871.1|AE013933_8 (AE013933) glutathione synthetase [Yersinia pestis KIM]
          Length = 319

 Score =  413 bits (1062), Expect = e-115
 Identities = 207/314 (65%), Positives = 244/314 (77%), Gaps = 1/314 (0%)






 
Structural Features:
COG0189, RimK, Glutathione synthase/Ribosomal protein S6 modification enzyme 
(glutaminyl transferase) 
[Coenzyme metabolism / Translation, ribosomal structure and biogenesis]
CD-Length = 318 residues,  99.7% aligned
Score =  278 bits (711), Expect = 7e-76
Identities = 136/326 (41%), Positives = 186/326 (57%), Gaps = 21/326 (6%)

pfam02955, GSH-S_ATP, Prokaryotic glutathione synthetase, ATP-binding domain.
CD-Length = 181 residues,  96.1% aligned
Score =  140 bits (355), Expect = 1e-34
Identities = 65/190 (34%), Positives = 82/190 (43%), Gaps = 24/190 (12%)

pfam02951, GSH-S_N, Prokaryotic glutathione synthetase, N-terminal domain.
CD-Length = 128 residues, 100.0% aligned
Score =  128 bits (323), Expect = 8e-31
Identities = 48/128 (37%), Positives = 72/128 (56%), Gaps = 9/128 (7%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4515




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21661




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33734




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2256_cdd.html


    		




 
URL:

















    ReferenceID:35379




  
Author/Investigator(s):
        Okumura N, Masamoto K, Wada H.





  
Title:
        The gshB gene in the cyanobacterium Synechococcus sp. PCC 7942 encodes a functional glutathione synthetase.




  
PubMed:
        9308172


MedLine:
        




    
Source:
Microbiology. 1997 Sep;143 ( Pt 9):2883-90.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9308172









           
Homologs By Global Alignment 

           

       



     
Gene ID:2256
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3840
                           PA0407_tr
                           translation of PA0407
                           317
                           99.99
                           99.99
                           0
                           1633.0
     

     
     
117804
                           gb|AAG03796.1|AE004478_1
                           (AE004478) glutathione synthetase [Pseudomonas aeruginosa PAO1]
                           317
                           99.99
                           99.99
                           0
                           1633.0
     

     
     
117805
                           gb|AAN70559.1|AE016698_7
                           (AE016792) glutathione synthetase [Pseudomonas putida KT2440]
                           317
                           82.96
                           88.01
                           0
                           1342.0
     

     
     
117806
                           gb|AAO58463.1|
                           (AE016874) glutathione synthetase [Pseudomonas syringae pv. tomato str. DC3000]
                           319
                           79.31
                           87.77
                           2
                           1322.0
     

     
     
117808
                           emb|CAC89778.1|
                           (AJ414145) glutathione synthetase [Yersinia pestis CO92]
                           320
                           64.68
                           76.56
                           4
                           1063.0
     

     






      

          
GC ORFID: 45258How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0407Glimmer Score: 

          
Start: 6265464Stop: 6264511

          
Length: 954


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0407 translation of PA0407Homolog Bit Score 633.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3709 (ORF: BLASTX 520.0), t_PA0407 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGCGTACGCCTCGGGATCGTCATGGACCCCATCGCGCGCATCAACTTCAAGAAGGACAGCTCGCTCGCC 
ATGCTGCTGGCTGCCCAGGCCCGCGGCTGGTCGCTGTTCTACATGGAACAGCAGGACCTCTACCAGAAGGCC 
GGCGTCGCGCGTGGACGCATGCGCCCGCTGAAGGTCTTCAACGATGCCTCCCGCTGGTTCGAACTGGAAGCG 
GAAAGCGACCAACCGCTGCACGAACTGGACGTGATCCTGATGCGCAAGGACCCGCCCTTCGATAACGAATTC 
GTCTATTCGACCTACCTGCTGGAACAGGCCGAGCGTGCCGGCGCCCTGGTGGTGAACCGGCCGCAGAGCCTG 
CGCGACTGCAACGAGAAGTTCTTCGCCACGCAGTTCACCCAGTGCACTCCGCCGACGATGGTCAGCCGGCGA 
TCCGATATTCTGCGCGAGTTTGCCGCCGAGCATCGTGACATCATTCTCAAACCCCTGGACGGAATGGGCGGT 
TCGTCGATATTTCGTCACCGCGAAGGCGACCCGAACCTCTCGGTGATCCTCGAGACCCTCACCCAGCATGGC 
AGCCAGCAGATCATGGCGCAGCGCTATCTGCCGGAAATCAAGGACGGCGACAAACGCATCCTGATGATCGAC 
GGCGAACCGGTGCCCTACTGCCTGGCGCGCATCCCCGCCCAGGGCGAGACCCGCGGCAACCTCGCCGCCGGC 
GGCCGCGGCGTGGCCCAGCCGCTGAGCGAGCGCGACCGCTGGATCGCCGCCGAGGTCGGCCCGCACCTGCGC 
GAGCGCGGCCTGCTGTTCGTCGGCCTCGACGTGATCGGCGATTATCTGACGGAAATCAACGTCACCAGCCCG 
ACCTGCATCCGCGAGATCGACCAGGCCTTCGACACCCGCATCGGCGACAAGCTGATGGACGCCATCGCCGCC 
CAGCTGGCTGCCCGCTGA