ViewGene
Locus Name:
GeneID:
Location:

GeneID:2253
TrivialName: PA14_25640
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_25640
PAO1 Ortholog Locus:PA2969
Sequence Length:981
Protein Length:326
Start: 2241175
Stop: 2242155
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06357.1|AE004723_1 (AE004723) fatty acid biosynthesis protein PlsX [Pseudomonas aeruginosa PAO1]
Identities = 326/326 (100%), Positives = 326/326 (100%)
Sequence:
ATGGGTGGGGACTTCGGTCCCCACTGCGTTGTTCCGGCCTCTATTGCCTTCCTGGCCGAGAATTCCTCTTCG
CAACTGATCCTCGTCGGTCAGCCTGCCCTCCTCGAAGAACTCCTTTCCCGCTATCCGTCGCTCGACCGGCAG
CGCCTGCGCGTGCATGCGGCCAGCGAAGTGATAGGTTCAGACGAACGTCCCTCCCAGGCCTTGCGTGGCAAG
CCGGACGCCTCCATGCGCGTGGCGCTGGAGCTGGTCCGCGACGGCCGGGCGCACGCGTGCGTCAGCGCCGGC
AATACCGGGGCGTTGATGGCGCTGTCGCGGCACCTGCTGAAGACCCTGCCCGGTATCGACCGCCCTGCGATG
GTGACGGCGGTTCCGACCGAGAAGGGGCATTGTCATCTGCTCGACCTCGGCGCCAACGTGGATTGCAGTGCC
GAGCATCTCTACCAGTTCGCGGTGATGGGCGCGGTGGCGGCAGAGGCCTTGGGCTGCGTCTCGCCCCGGGTC
GCCCTGCTCAACGTGGGGACCGAGGAGATCAAGGGCAACCAGCAGGTCAAGCTGGCGGCGAGCCTGTTGCAG
AAGGCGCAGGGGTTGAATTTCAGCGGATATATAGAGGGCGATGGCCTGTATCGCGGCGAGGCCGACGTGGTG
GTCTGCGACGGTTTCGTCGGCAACATCCTGCTGAAGGCCAGCGAAGGCCTGGCTGCCATGGTGTCGGCGCGG
ATCGAACAGCGCTTCCGCGATGGACTGGCGGCGAAGCTGGTCGGTGCGCTGGCGTTGCCGCTGCTGCGCCGG
CTGCGCGGCGATATCGCTCCGGCACGCTATAACGGCGCCAGTTTCCTCGGATTGCAGGGGATAGTGGTGAAA
AGCCATGGGTCTGCCGCCGAGGAGGGTTTCCAGAGCGCCTTGCGTCGCGCGGCACTGGAAGTCCGGGAAAAC
CTGCCGCAACGATTGCATGGCCGTCTCGAGCATCTGCTGCTCTAA
Translation:
MGGDFGPHCVVPASIAFLAENSSSQLILVGQPALLEELLSRYPSLDRQRLRVHAASEVIGSDERPSQALRGK
PDASMRVALELVRDGRAHACVSAGNTGALMALSRHLLKTLPGIDRPAMVTAVPTEKGHCHLLDLGANVDCSA
EHLYQFAVMGAVAAEALGCVSPRVALLNVGTEEIKGNQQVKLAASLLQKAQGLNFSGYIEGDGLYRGEADVV
VCDGFVGNILLKASEGLAAMVSARIEQRFRDGLAAKLVGALALPLLRRLRGDIAPARYNGASFLGLQGIVVK
SHGSAAEEGFQSALRRAALEVRENLPQRLHGRLEHLLL*
AnnotationID:2261GeneID:2253
AnnotatorUID: danlee
Modification Date/Time: 2006-01-31 14:02:37
Gene Name:plsX
Confidence Code:2
GeneProduct:fatty acid/phospholipid synthesis protein
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0416,pfam02504
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN67530.1|AE016381_7 (AE016781) fatty acid/phospholipid synthesis protein PlsX
           [Pseudomonas putida KT2440]
          Length = 336

 Score =  488 bits (1257), Expect = e-137
 Identities = 243/325 (74%), Positives = 287/325 (88%)

>gb|AAO57302.1| (AE016869) fatty acid/phospholipid synthesis protein PlsX
           [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 330

 Score =  454 bits (1169), Expect = e-127
 Identities = 232/322 (72%), Positives = 269/322 (83%)

>gb|AAO90040.1| (AE016961) fatty acid/phospholipid synthesis protein PlsX [Coxiella
           burnetii RSA 493]
          Length = 343

 Score =  313 bits (801), Expect = 1e-84
 Identities = 169/331 (51%), Positives = 232/331 (70%), Gaps = 9/331 (2%)








 
Structural Features:
COG0416, PlsX, Fatty acid/phospholipid biosynthesis enzyme [Lipid metabolism]

pfam02504, FA_synthesis, Fatty acid synthesis protein.








   
Genomic Context:






   
Comment:


















    ReferenceID:4509




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21655




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36081




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2253_cdd.html


    		




 
URL:

















    ReferenceID:36443




  
Author/Investigator(s):
        Kutchma AJ, Hoang TT, Schweizer HP.





  
Title:
        Characterization of a Pseudomonas aeruginosa fatty acid biosynthetic gene cluster: purification of acyl carrier protein (ACP) and malonyl-coenzyme A:ACP transacylase (FabD).




  
PubMed:
        10464226


MedLine:
        




    
Source:
J Bacteriol. 1999 Sep;181(17):5498-504.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10464226









           
Homologs By Global Alignment 

           

       



     
Gene ID:2253
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3832
                           PA2969_tr
                           translation of PA2969
                           326
                           99.99
                           99.99
                           0
                           1632.0
     

     
     
117726
                           gb|AAG06357.1|AE004723_1
                           (AE004723) fatty acid biosynthesis protein PlsX [Pseudomonas aeruginosa PAO1]
                           326
                           99.99
                           99.99
                           0
                           1632.0
     

     
     
117727
                           gb|AAN67530.1|AE016381_7
                           (AE016781) fatty acid/phospholipid synthesis protein PlsX [Pseudomonas putida KT2440]
                           336
                           72.32
                           85.41
                           10
                           1254.0
     

     
     
117728
                           gb|AAO57302.1|
                           (AE016869) fatty acid/phospholipid synthesis protein PlsX [Pseudomonas syringae pv. tomato str. DC3000]
                           330
                           70.30
                           81.81
                           4
                           1167.0
     

     
     
117730
                           gb|AAO90040.1|
                           (AE016961) fatty acid/phospholipid synthesis protein PlsX [Coxiella burnetii RSA 493]
                           346
                           48.55
                           67.05
                           23
                           809.0
     

     






      

          
GC ORFID: 45255How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2969Glimmer Score: 

          
Start: 2712750Stop: 2711770

          
Length: 981


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAATruncated Stop: 

          
Homolog: t_PA2969 translation of PA2969Homolog Bit Score 633.0

          
Other Homologs:  psyr_15may02_Scaffold13_revised_gene2160 (ORF: BLASTX 462.0), t_PA2969 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGGTGGGGACTTCGGTCCCCACTGCGTTGTTCCGGCCTCTATTGCCTTCCTGGCCGAGAATTCCTCTTCG 
CAACTGATCCTCGTCGGTCAGCCTGCCCTCCTCGAAGAACTCCTTTCCCGCTATCCGTCGCTCGACCGGCAG 
CGCCTGCGCGTGCATGCGGCCAGCGAAGTGATAGGTTCAGACGAACGTCCCTCCCAGGCCTTGCGTGGCAAG 
CCGGACGCCTCCATGCGCGTGGCGCTGGAGCTGGTCCGCGACGGCCGGGCGCACGCGTGCGTCAGCGCCGGC 
AATACCGGGGCGTTGATGGCGCTGTCGCGGCACCTGCTGAAGACCCTGCCCGGTATCGACCGCCCTGCGATG 
GTGACGGCGGTTCCGACCGAGAAGGGGCATTGTCATCTGCTCGACCTCGGCGCCAACGTGGATTGCAGTGCC 
GAGCATCTCTACCAGTTCGCGGTGATGGGCGCGGTGGCGGCAGAGGCCTTGGGCTGCGTCTCGCCCCGGGTC 
GCCCTGCTCAACGTGGGGACCGAGGAGATCAAGGGCAACCAGCAGGTCAAGCTGGCGGCGAGCCTGTTGCAG 
AAGGCGCAGGGGTTGAATTTCAGCGGATATATAGAGGGCGATGGCCTGTATCGCGGCGAGGCCGACGTGGTG 
GTCTGCGACGGTTTCGTCGGCAACATCCTGCTGAAGGCCAGCGAAGGCCTGGCTGCCATGGTGTCGGCGCGG 
ATCGAACAGCGCTTCCGCGATGGACTGGCGGCGAAGCTGGTCGGTGCGCTGGCGTTGCCGCTGCTGCGCCGG 
CTGCGCGGCGATATCGCTCCGGCACGCTATAACGGCGCCAGTTTCCTCGGATTGCAGGGGATAGTGGTGAAA 
AGCCATGGGTCTGCCGCCGAGGAGGGTTTCCAGAGCGCCTTGCGTCGCGCGGCACTGGAAGTCCGGGAAAAC 
CTGCCGCAACGATTGCATGGCCGTCTCGAGCATCTGCTGCTCTAA