ViewGene
Locus Name:
GeneID:
Location:

GeneID:2243
TrivialName: PA14_13500
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_13500
PAO1 Ortholog Locus:PA3896
Sequence Length:978
Protein Length:325
Start: 1161378
Stop: 1160401
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07283.1|AE004807_2 (AE004807) probable 2-hydroxyacid dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 323/325 (99%), Positives = 324/325 (99%)
Sequence:
ATGAAAAAGAACGTCTTCGTCTTCAGTCGCCTCGCCCCCGAGCATCTCGAACGCCTGCAACGCCAGTTCAAC
GTCCGCGTGCTCGAACCGAAACAGGGCGACATCGACGCCCAGTACGCCGCCGCCCTGCCCGATACCCACGGC
ATGATCGGCGTCGGCCGGCCGCTCGGCGCCAGGCAACTGGAACAGGCGAAGCAGCTGGAAGTCATCTCCAGC
GTCTCGGTCGGCTACGACAACTATGACCTGGACTACCTGAACCGCCGCGGCATCGCCCTCACCAACACGCCC
GACGTCCTCACCGAGACCACCGCCGACCTCGGCTTCGCCCTGCTGATCAGCGCCGCGCGACGGGTCGCCGAA
CTGGACGCCTGGGTCAAGGCCGGGAACTGGAAGCGCACCGTCGATGCCCCGCAGTTCGGCACCGATGTGCAT
GGCAAGAAACTCGGCATCCTCGGCCTCGGCCGGATCGGCGCGGCCATCGCCCGGCGCGGCCGTTTCGGCTTC
GGCATGCAGGTGCTCTACCACGGCAACAACCGCAAGCCGGAACTGGAACAGGAACTCGGCGCGCGCTTCCTC
GGCTTCGACGAACTGCTCGGCGAGGCCGACTTCGTCTGCGTGGTGGTGCCGCTGGGAGCGCAGACCCGGCGG
TTGATCGGCGCGCGCGAACTGGGCCTGATGAAGCCCTCGGCGATCCTGGTCAACGTCGCCCGCGGCCAGGTG
GTCGACGAGGCCGCGCTGGTCGCCGCCCTGCGGGAGAAACGCATCCTCGGCGCCGGCCTGGATGTCTACGAG
AAGGAACCGCTGGCCGAATCGCCGCTGTTCGCCCTGGACAACGTGGTGACCCTGCCGCACATCGGCTCGGCC
ACCCACGAGACCCGCCGGGCCATGGCCGAGCGGGCGCTGCAGAACTTCGAGGCGGCCCTGCGCGGCGAGCGG
CCGCTGGACCTGGTCAATCCGCAGGTGTGGCGACGCGGCTGA
Translation:
MKKNVFVFSRLAPEHLERLQRQFNVRVLEPKQGDIDAQYAAALPDTHGMIGVGRPLGARQLEQAKQLEVISS
VSVGYDNYDLDYLNRRGIALTNTPDVLTETTADLGFALLISAARRVAELDAWVKAGNWKRTVDAPQFGTDVH
GKKLGILGLGRIGAAIARRGRFGFGMQVLYHGNNRKPELEQELGARFLGFDELLGEADFVCVVVPLGAQTRR
LIGARELGLMKPSAILVNVARGQVVDEAALVAALREKRILGAGLDVYEKEPLAESPLFALDNVVTLPHIGSA
THETRRAMAERALQNFEAALRGERPLDLVNPQVWRRG*
AnnotationID:2251GeneID:2243
AnnotatorUID: diggins
Modification Date/Time: 2005-04-25 20:08:21
Gene Name:
Confidence Code:3
GeneProduct:putative D-isomer specific 2-hydroxyacid dehydroge
Cell Localization:()
Synonyms:PA3896
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(12) Energy metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs: COG1052,COG0111
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO54742.1| (AE016860) D-isomer specific 2-hydroxyacid dehydrogenase family
           protein [Pseudomonas syringae pv. tomato str. DC3000]
          Length = 324

 Score =  451 bits (1161), Expect = e-126
 Identities = 223/323 (69%), Positives = 262/323 (81%)


>gb|AAN68980.1|AE016530_3 (AE016786) 2-ketogluconate 6-phosphate reductase [Pseudomonas
           putida KT2440]
          Length = 320

 Score =  351 bits (900), Expect = 4e-96
 Identities = 184/319 (57%), Positives = 227/319 (71%), Gaps = 1/319 (0%)






 
Structural Features:
COG1052, LdhA, Lactate dehydrogenase and related dehydrogenases 
[Energy production and conversion / Coenzyme metabolism]
CD-Length = 324 residues, 100.0% aligned
Score =  320 bits (822), Expect = 2e-88
Identities = 155/326 (47%), Positives = 199/326 (61%), Gaps = 8/326 (2%)

COG0111, SerA, Phosphoglycerate dehydrogenase and related dehydrogenases 
[Amino acid transport and metabolism]
CD-Length = 324 residues,  97.2% aligned
Score =  252 bits (644), Expect = 1e-67
Identities = 127/320 (39%), Positives = 174/320 (54%), Gaps = 7/320 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4489




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21635




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:31670




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2243_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2243
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3802
                           PA3896_tr
                           translation of PA3896
                           325
                           99.38
                           99.69
                           0
                           1637.0
     

     
     
117059
                           gb|AAG07283.1|AE004807_2
                           (AE004807) probable 2-hydroxyacid dehydrogenase [Pseudomonas aeruginosa PAO1]
                           325
                           99.38
                           99.69
                           0
                           1637.0
     

     
     
117060
                           gb|AAO54742.1|
                           (AE016860) D-isomer specific 2-hydroxyacid dehydrogenase family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           325
                           68.61
                           80.61
                           1
                           1159.0
     

     
     
117061
                           gb|AAN66885.1|AE016317_2
                           (AE016778) D-isomer specific 2-hydroxyacid dehydrogenase family protein [Pseudomonas putida KT2440]
                           325
                           68.00
                           78.46
                           1
                           1136.0
     

     
     
117063
                           gb|AAK37650.1|
                           (AF012100) KguD [Pseudomonas aeruginosa]
                           329
                           56.83
                           72.34
                           5
                           925.0
     

     






      

          
GC ORFID: 45245How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3896Glimmer Score: 

          
Start: 3792560Stop: 3793537

          
Length: 978


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3896 translation of PA3896Homolog Bit Score 635.0

          
Other Homologs:  psyr_15may02_Scaffold3_revised_gene5011 (ORF: BLASTX 452.0), t_PA2263 (ORF: BLASTX 359.0), t_PA3896 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAAAAAGAACGTCTTCGTCTTCAGTCGCCTCGCCCCCGAGCATCTCGAACGCCTGCAACGCCAGTTCAAC 
GTCCGCGTGCTCGAACCGAAACAGGGCGACATCGACGCCCAGTACGCCGCCGCCCTGCCCGATACCCACGGC 
ATGATCGGCGTCGGCCGGCCGCTCGGCGCCAGGCAACTGGAACAGGCGAAGCAGCTGGAAGTCATCTCCAGC 
GTCTCGGTCGGCTACGACAACTATGACCTGGACTACCTGAACCGCCGCGGCATCGCCCTCACCAACACGCCC 
GACGTCCTCACCGAGACCACCGCCGACCTCGGCTTCGCCCTGCTGATCAGCGCCGCGCGACGGGTCGCCGAA 
CTGGACGCCTGGGTCAAGGCCGGGAACTGGAAGCGCACCGTCGATGCCCCGCAGTTCGGCACCGATGTGCAT 
GGCAAGAAACTCGGCATCCTCGGCCTCGGCCGGATCGGCGCGGCCATCGCCCGGCGCGGCCGTTTCGGCTTC 
GGCATGCAGGTGCTCTACCACGGCAACAACCGCAAGCCGGAACTGGAACAGGAACTCGGCGCGCGCTTCCTC 
GGCTTCGACGAACTGCTCGGCGAGGCCGACTTCGTCTGCGTGGTGGTGCCGCTGGGAGCGCAGACCCGGCGG 
TTGATCGGCGCGCGCGAACTGGGCCTGATGAAGCCCTCGGCGATCCTGGTCAACGTCGCCCGCGGCCAGGTG 
GTCGACGAGGCCGCGCTGGTCGCCGCCCTGCGGGAGAAACGCATCCTCGGCGCCGGCCTGGATGTCTACGAG 
AAGGAACCGCTGGCCGAATCGCCGCTGTTCGCCCTGGACAACGTGGTGACCCTGCCGCACATCGGCTCGGCC 
ACCCACGAGACCCGCCGGGCCATGGCCGAGCGGGCGCTGCAGAACTTCGAGGCGGCCCTGCGCGGCGAGCGG 
CCGCTGGACCTGGTCAATCCGCAGGTGTGGCGACGCGGCTGA