ViewGene
Locus Name:
GeneID:
Location:

GeneID:2241
TrivialName: PA14_45800
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_45800
PAO1 Ortholog Locus:PA1443
Sequence Length:972
Protein Length:323
Start: 4071677
Stop: 4070706
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04832.1|AE004574_3 (AE004574) flagellar motor switch protein FliM [Pseudomonas aeruginosa PAO1]
Identities = 323/323 (100%), Positives = 323/323 (100%)
Sequence:
ATGGCCGTGCAAGATCTGCTTTCCCAGGATGAAATCGACGCGCTGTTGCACGGCGTCGACGATGGCCTGGTG
GAAACCGAAGTCGAGGCGACGCCGGGATCGGTCAAGTCCTATGACCTGACCAGCCAGGACCGCATCGTCCGG
GGCCGCATGCCGACCCTGGAGATGATCAACGAGCGTTTCGCCCGCTACACCCGCATCAGCATGTTCAACCTG
CTGCGCCGTTCGGCCGACGTCGCGGTCGGCGGCGTGCAGGTGATGAAGTTCGGCGAGTACGTGCACTCGCTG
TACGTTCCCACCAGCCTCAACCTGGTGAAGATGAAGCCGCTGCGCGGCACCGCGCTGTTCATCCTCGACGCC
AAGCTGGTGTTCAAGCTGGTGGACAACTTCTTCGGCGGCGACGGCCGCCACGCCAAGATCGAGGGCCGCGAG
TTCACCCCGACCGAACTGCGGGTGGTGCGGATGGTGCTGGAGCAGGCCTTCGTCGATCTGAAGGAAGCCTGG
CAGGCGGTGCTGGAGATGAACTTCGAGTACGTCAACTCGGAGGTCAACCCGGCCATGGCCAACATCGTCAGT
CCCAGCGAGGTGGTGGTGGTCTCCACCTTCCACATCGAACTGGACGGCGGTGGCGGCGACCTGCACATCACC
ATGCCCTATTCGATGATCGAGCCGATCCGCGAGATGCTCGACGCCGGTTTCCAGTCCGACCACGACGACCAG
GACGAGCGCTGGATCAAGGCCCTGCGCGAGGACGTGCTGGACGTCCAGGTACCGCTCGGCGCCACCGTGGTC
CGCCGCCAGCTCAAGCTGCGCGACATCCTGCACATGCAGCCGGGCGACGTGATCCCGGTGGAGATGCCGGAA
CACATGGTGATGCGCGCCAACGGCGTGCCCGCCTTCAAGGTCAAGCTGGGCGCCCACAAGGGCAACCTGGCG
CTACAGATTCTCGAAGCGGTCGAGCGCTCGCGCTGA
Translation:
MAVQDLLSQDEIDALLHGVDDGLVETEVEATPGSVKSYDLTSQDRIVRGRMPTLEMINERFARYTRISMFNL
LRRSADVAVGGVQVMKFGEYVHSLYVPTSLNLVKMKPLRGTALFILDAKLVFKLVDNFFGGDGRHAKIEGRE
FTPTELRVVRMVLEQAFVDLKEAWQAVLEMNFEYVNSEVNPAMANIVSPSEVVVVSTFHIELDGGGGDLHIT
MPYSMIEPIREMLDAGFQSDHDDQDERWIKALREDVLDVQVPLGATVVRRQLKLRDILHMQPGDVIPVEMPE
HMVMRANGVPAFKVKLGAHKGNLALQILEAVERSR*
AnnotationID:2249GeneID:2241
AnnotatorUID: diggins
Modification Date/Time: 2005-02-24 09:09:22
Gene Name:fliM
Confidence Code:2
GeneProduct:flagellar motor switch protein FliM
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(16) Motility & Attachment
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1868,pfam02154
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN69936.1|AE016632_7 (AE016790) flagellar motor switch protein FliM [Pseudomonas putida
           KT2440]
          Length = 322

 Score =  581 bits (1497), Expect = e-165
 Identities = 285/321 (88%), Positives = 308/321 (95%)

>gb|AAN56220.1|AE015759_12 (AE015759) flagellar motor switch protein FliM [Shewanella
           oneidensis MR-1]
          Length = 343

 Score =  422 bits (1086), Expect = e-117
 Identities = 198/320 (61%), Positives = 266/320 (83%), Gaps = 1/320 (0%)

>gb|AAO10342.1|AE016803_129 (AE016803) Polar flagellar switch protein [Vibrio vulnificus CMCP6]
          Length = 347

 Score =  407 bits (1046), Expect = e-113
 Identities = 194/319 (60%), Positives = 258/319 (80%), Gaps = 2/319 (0%)






 
Structural Features:
COG1868, FliM, Flagellar motor switch protein [Cell motility and secretion]
CD-Length = 332 residues,  96.7% aligned
Score =  364 bits (935), Expect = 1e-101
Identities = 151/322 (46%), Positives = 198/322 (61%), Gaps = 4/322 (1%)

pfam02154, FliM, Flagellar motor switch protein FliM.
CD-Length = 192 residues, 100.0% aligned
Score =  241 bits (617), Expect = 7e-65
Identities = 89/192 (46%), Positives = 126/192 (65%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4485




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21631




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:37363




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2241_cdd.html


    		




 
URL:

















    ReferenceID:37986




  
Author/Investigator(s):
        Hardham JM, Frye JG, Stamm LV.





  
Title:
        Identification and sequences of the Treponema pallidum fliM', fliY, fliP, fliQ, fliR and flhB' genes.




  
PubMed:
        8529894


MedLine:
        




    
Source:
Gene. 1995 Dec 1;166(1):57-64.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8529894

















    ReferenceID:37987




  
Author/Investigator(s):
        Zuberi AR, Bischoff DS, Ordal GW.





  
Title:
        Nucleotide sequence and characterization of a Bacillus subtilis gene encoding a flagellar switch protein.




  
PubMed:
        1898932


MedLine:
        




    
Source:
J Bacteriol. 1991 Jan;173(2):710-9.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1898932

















    ReferenceID:37988




  
Author/Investigator(s):
        Yu J, Shapiro L.





  
Title:
        Early Caulobacter crescentus genes fliL and fliM are required for flagellar gene expression and normal cell division.




  
PubMed:
        1315735


MedLine:
        




    
Source:
J Bacteriol. 1992 May;174(10):3327-38.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=1315735









           
Homologs By Global Alignment 

           

       



     
Gene ID:2241
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3800
                           PA1443_tr
                           translation of PA1443
                           323
                           99.99
                           99.99
                           0
                           1638.0
     

     
     
116993
                           gb|AAG04832.1|AE004574_3
                           (AE004574) flagellar motor switch protein FliM [Pseudomonas aeruginosa PAO1]
                           323
                           99.99
                           99.99
                           0
                           1638.0
     

     
     
116994
                           gb|AAD56294.1|
                           (AF031418) FliM [Pseudomonas putida]
                           323
                           88.23
                           95.35
                           1
                           1496.0
     

     
     
116995
                           gb|AAN69936.1|AE016632_7
                           (AE016790) flagellar motor switch protein FliM [Pseudomonas putida KT2440]
                           323
                           88.23
                           95.35
                           1
                           1496.0
     

     
     
116996
                           gb|AAO55487.1|
                           (AE016862) flagellar motor switch protein FliM [Pseudomonas syringae pv. tomato str. DC3000]
                           323
                           85.13
                           94.11
                           1
                           1459.0
     

     






      

          
GC ORFID: 45243How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA1443Glimmer Score: 

          
Start: 882232Stop: 883203

          
Length: 972


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA1443 translation of PA1443Homolog Bit Score 635.0

          
Other Homologs:  psyr_15may02_Scaffold8_revised_gene7928 (ORF: BLASTX 566.0), t_PA1443 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGGCCGTGCAAGATCTGCTTTCCCAGGATGAAATCGACGCGCTGTTGCACGGCGTCGACGATGGCCTGGTG 
GAAACCGAAGTCGAGGCGACGCCGGGATCGGTCAAGTCCTATGACCTGACCAGCCAGGACCGCATCGTCCGG 
GGCCGCATGCCGACCCTGGAGATGATCAACGAGCGTTTCGCCCGCTACACCCGCATCAGCATGTTCAACCTG 
CTGCGCCGTTCGGCCGACGTCGCGGTCGGCGGCGTGCAGGTGATGAAGTTCGGCGAGTACGTGCACTCGCTG 
TACGTTCCCACCAGCCTCAACCTGGTGAAGATGAAGCCGCTGCGCGGCACCGCGCTGTTCATCCTCGACGCC 
AAGCTGGTGTTCAAGCTGGTGGACAACTTCTTCGGCGGCGACGGCCGCCACGCCAAGATCGAGGGCCGCGAG 
TTCACCCCGACCGAACTGCGGGTGGTGCGGATGGTGCTGGAGCAGGCCTTCGTCGATCTGAAGGAAGCCTGG 
CAGGCGGTGCTGGAGATGAACTTCGAGTACGTCAACTCGGAGGTCAACCCGGCCATGGCCAACATCGTCAGT 
CCCAGCGAGGTGGTGGTGGTCTCCACCTTCCACATCGAACTGGACGGCGGTGGCGGCGACCTGCACATCACC 
ATGCCCTATTCGATGATCGAGCCGATCCGCGAGATGCTCGACGCCGGTTTCCAGTCCGACCACGACGACCAG 
GACGAGCGCTGGATCAAGGCCCTGCGCGAGGACGTGCTGGACGTCCAGGTACCGCTCGGCGCCACCGTGGTC 
CGCCGCCAGCTCAAGCTGCGCGACATCCTGCACATGCAGCCGGGCGACGTGATCCCGGTGGAGATGCCGGAA 
CACATGGTGATGCGCGCCAACGGCGTGCCCGCCTTCAAGGTCAAGCTGGGCGCCCACAAGGGCAACCTGGCG 
CTACAGATTCTCGAAGCGGTCGAGCGCTCGCGCTGA