ViewGene
Locus Name:
GeneID:
Location:

GeneID:2235
TrivialName: PA14_17910
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_17910
PAO1 Ortholog Locus:PA3586
Sequence Length:987
Protein Length:328
Start: 1538565
Stop: 1539551
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06974.1|AE004779_6 (AE004779) probable hydrolase [Pseudomonas aeruginosa PAO1]
Identities = 328/328 (100%), Positives = 328/328 (100%)
Sequence:
ATGCGTGCTTTCATCTTCCTGGCCGCCTTGTTGTGCAGCCTCTCCTCCTTTGCGGCGGCGCGTTGCGACGCA
CGCGTACCTACCGAGACGGTCGAACTGGGCGACGTGCGCCTGGCCTACCAGAGCATCGGCCGCGACAGCGAT
CCGGCCCTGCTGCTGGTGATGGGCCTGGGCGGGCAACTGATCCACTGGCCGGACGAGGTGGTCAGCGCCCTG
TGCGAGCAAGGCTTCCGGGTGATCCGCTACGACAACCGTGACGTCGGCCTGTCGGCCTGGAACGTGCCGGTG
CCCAGCTCGCGGCTGACCTACGAAGTGGTGCGTTATCGCCTCGGCCTGCCGGTCAGCGCGCCCTATACCCTC
ACCGACATGGCCGGCGATGCCCTGCATCTGCTCGACGCGCTGGATATCCCCCAGGCCCATGTGCTCGGCGCC
AGCATGGGCGGGATGATCGCCCAGCACATCGCCGACATGGCGCCGCAGCGCCTGCTCAGCCTGACCCTGGTG
ATGACCAGCTCCGGCGCCGAAGGCCTGCCGGCGCCCAGCGAGTCGCTGCTGCGCCTGCTGGCGCGGCGCGAG
GCGGCCAGCCGCGAGCAGGCGGTCGAGCAGCAGGCCGACCTGCTGGCGGCGCTGGGCAGCCCGGAAGTGCGC
GACGATCGCCAGCAGCTCCTGTTGCAGGCCGCACGTTCCTACGACCGCGCGTTCAACCCGGAAGGCGTGCAG
CGGCAATTGCTGGCAATCCTCGCCGAACCCAGCCGGGTGCCGCTGCTGAACCGTCTGCAGGTGCCGACCCTG
GTGATCCATGGCACCGCCGATCCGCTGCTGCCGGTGATGCACGGCGTGCACGTGGCGGCGCATATCCGTGGC
TCGGAGCTGAAGCTGATCCCCGGTCTCGCCCATCGCTTCCAGGAAGCCTTCAAGGAGCCGTTGCTGGCGGCG
GTGGTGCCTTACCTGAAGGCCCATCGGCAGGCCAGCGTCGTCCAGCTCTGA
Translation:
MRAFIFLAALLCSLSSFAAARCDARVPTETVELGDVRLAYQSIGRDSDPALLLVMGLGGQLIHWPDEVVSAL
CEQGFRVIRYDNRDVGLSAWNVPVPSSRLTYEVVRYRLGLPVSAPYTLTDMAGDALHLLDALDIPQAHVLGA
SMGGMIAQHIADMAPQRLLSLTLVMTSSGAEGLPAPSESLLRLLARREAASREQAVEQQADLLAALGSPEVR
DDRQQLLLQAARSYDRAFNPEGVQRQLLAILAEPSRVPLLNRLQVPTLVIHGTADPLLPVMHGVHVAAHIRG
SELKLIPGLAHRFQEAFKEPLLAAVVPYLKAHRQASVVQL*
AnnotationID:2243GeneID:2235
AnnotatorUID: diggins
Modification Date/Time: 2005-04-20 11:11:59
Gene Name:
Confidence Code:3
GeneProduct:putative hydrolase, alpha/beta fold family
Cell Localization:()
Synonyms:lipG
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:pfam00561,COG0596
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57634.1| (AE016871) hydrolase, alpha/beta fold family [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 330

 Score =  484 bits (1246), Expect = e-136
 Identities = 243/322 (75%), Positives = 279/322 (86%)

>gb|AAK76842.1|AE001438_95 (AE001438) Carboxyl esterase, a/b hydrolase [Clostridium
           acetobutylicum]
          Length = 299

 Score =  176 bits (447), Expect = 1e-43
 Identities = 103/290 (35%), Positives = 154/290 (53%), Gaps = 4/290 (1%)







 
Structural Features:
pfam00561, Abhydrolase_1, alpha/beta hydrolase fold. 
This catalytic domain is found in a very wide range of enzymes.
CD-Length = 224 residues,  94.6% aligned
Score = 85.6 bits (211), Expect = 7e-18
Identities = 70/233 (30%), Positives = 97/233 (41%), Gaps = 27/233 (11%)

COG0596, MhpC, Predicted hydrolases or acyltransferases
CD-Length = 282 residues, 100.0% aligned
Score = 83.6 bits (204), Expect = 3e-17






   
Genomic Context:






   
Comment:


















    ReferenceID:4473




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21619




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:38103




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2235_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2235
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3793
                           PA3586_tr
                           translation of PA3586
                           328
                           99.99
                           99.99
                           0
                           1641.0
     

     
     
116950
                           gb|AAG06974.1|AE004779_6
                           (AE004779) probable hydrolase [Pseudomonas aeruginosa PAO1]
                           328
                           99.99
                           99.99
                           0
                           1641.0
     

     
     
116951
                           gb|AAO57634.1|
                           (AE016871) hydrolase, alpha/beta fold family [Pseudomonas syringae pv. tomato str. DC3000]
                           330
                           74.24
                           85.75
                           2
                           1245.0
     

     
     
116952
                           gb|AAN66689.1|AE016297_3
                           (AE016778) hydrolase, alpha/beta fold family [Pseudomonas putida KT2440]
                           379
                           63.32
                           73.35
                           52
                           1224.0
     

     
     
116958
                           emb|CAA86927.1|
                           (Z46863) estB [Acinetobacter sp. ADP1]
                           338
                           36.98
                           52.95
                           36
                           471.0
     

     






      

          
GC ORFID: 45237How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3586Glimmer Score: 

          
Start: 3415371Stop: 3414385

          
Length: 987


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3586 translation of PA3586Homolog Bit Score 636.0

          
Other Homologs:  psyr_15may02_Scaffold5_revised_gene6256 (ORF: BLASTX 491.0), t_PA3586 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCGTGCTTTCATCTTCCTGGCCGCCTTGTTGTGCAGCCTCTCCTCCTTTGCGGCGGCGCGTTGCGACGCA 
CGCGTACCTACCGAGACGGTCGAACTGGGCGACGTGCGCCTGGCCTACCAGAGCATCGGCCGCGACAGCGAT 
CCGGCCCTGCTGCTGGTGATGGGCCTGGGCGGGCAACTGATCCACTGGCCGGACGAGGTGGTCAGCGCCCTG 
TGCGAGCAAGGCTTCCGGGTGATCCGCTACGACAACCGTGACGTCGGCCTGTCGGCCTGGAACGTGCCGGTG 
CCCAGCTCGCGGCTGACCTACGAAGTGGTGCGTTATCGCCTCGGCCTGCCGGTCAGCGCGCCCTATACCCTC 
ACCGACATGGCCGGCGATGCCCTGCATCTGCTCGACGCGCTGGATATCCCCCAGGCCCATGTGCTCGGCGCC 
AGCATGGGCGGGATGATCGCCCAGCACATCGCCGACATGGCGCCGCAGCGCCTGCTCAGCCTGACCCTGGTG 
ATGACCAGCTCCGGCGCCGAAGGCCTGCCGGCGCCCAGCGAGTCGCTGCTGCGCCTGCTGGCGCGGCGCGAG 
GCGGCCAGCCGCGAGCAGGCGGTCGAGCAGCAGGCCGACCTGCTGGCGGCGCTGGGCAGCCCGGAAGTGCGC 
GACGATCGCCAGCAGCTCCTGTTGCAGGCCGCACGTTCCTACGACCGCGCGTTCAACCCGGAAGGCGTGCAG 
CGGCAATTGCTGGCAATCCTCGCCGAACCCAGCCGGGTGCCGCTGCTGAACCGTCTGCAGGTGCCGACCCTG 
GTGATCCATGGCACCGCCGATCCGCTGCTGCCGGTGATGCACGGCGTGCACGTGGCGGCGCATATCCGTGGC 
TCGGAGCTGAAGCTGATCCCCGGTCTCGCCCATCGCTTCCAGGAAGCCTTCAAGGAGCCGTTGCTGGCGGCG 
GTGGTGCCTTACCTGAAGGCCCATCGGCAGGCCAGCGTCGTCCAGCTCTGA