ViewGene
Locus Name:
GeneID:
Location:

GeneID:2234
TrivialName: PA14_24070
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_24070
PAO1 Ortholog Locus:PA3097
Sequence Length:1002
Protein Length:333
Start: 2089662
Stop: 2090663
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06485.1|AE004734_3 (AE004734) general secretion pathway protein K [Pseudomonas aeruginosa PAO1]
Identities = 321/333 (96%), Positives = 327/333 (98%)
Sequence:
ATGAGGCGCGGGCAGAGCGGCGTCGCGCTGATTACCGTGCTGCTGGTGGTGGCGGTGGTGACCATCGTCTGC
GCCGGGCTGATCATCCGCCAGCAGTTGGCGATACGCAGCAGCGCCAACCAGTTGCACGTGCGCCAGGCCTGG
CACTATGCGCTGGGCGGCGAACGCCTGGCGGAAGCGGTGCTGCGCCGCGACCTGCGCCAGGGCGGCGAGAAT
ACCCGCGAGCCGGTGGACCACCTGGGCGAGGCCTGGGCGCGACCGATGACGCCGTTCAAACTGGACGACGGC
GGCGAGCTGCGGGTGCGGATCGAGGACCCCAGCGGGCGTTTCAACCTCAACGGACTGGTGCGCAAGCGCAAG
GTCAAGCCGGATTCGGTGAAGCAGTTCCGTCGCCTGCTGGCCACCCTGGGGATGAAGGAAGAGATCGTTCAG
GGCTTGCCGGACCGGCTGGCCGACTGGCTCGACGCCGACCAGAATCCGCAGGGCGAGCAAGGCGCCGAGGAC
AACCAGTACCTGCTGGAGGCGCCGGCCTACCGCGCCGCCAACCGCAGTTTCAAGGACGTGTCCGAGCTGCGC
CTGCTGAAATTGTCGGAAGCCGACTATCGACGCCTGCTGCCGTTCGTCAGCGCCTTGCCCGAGGATGCGCCG
CTGAACGTGAACACCGCCAGCGTGCCGGTGCTGGCCGCCATGTTCGAGATCGATCCGGGACAGGCGGAAAAC
ATCGTGGCGGCTCGTGGGCGAGAAGGCTTCCAGGAGAAGACTGAGTTCAGCAAGCACCTGAACGAGTTGGGA
TCAAAGGCAAGTAACGTCAACTTTGCCGTCGGCACCCGCTACTTCCAGGTGATCAGCGAGGTCAGCCTGGGC
GACCGCCGGCAGGTGCTGGTGAGTACCTTGCAGCGGGGCAAGGACGGCAAGATACGCGTGATGGCCCGCGAC
ATGGGGCAGGGCGGCCTGCCCATCCCGTCGACGGGCGGCGACGATTGGAAGAAGGACGAGCGATGA
Translation:
MRRGQSGVALITVLLVVAVVTIVCAGLIIRQQLAIRSSANQLHVRQAWHYALGGERLAEAVLRRDLRQGGEN
TREPVDHLGEAWARPMTPFKLDDGGELRVRIEDPSGRFNLNGLVRKRKVKPDSVKQFRRLLATLGMKEEIVQ
GLPDRLADWLDADQNPQGEQGAEDNQYLLEAPAYRAANRSFKDVSELRLLKLSEADYRRLLPFVSALPEDAP
LNVNTASVPVLAAMFEIDPGQAENIVAARGREGFQEKTEFSKHLNELGSKASNVNFAVGTRYFQVISEVSLG
DRRQVLVSTLQRGKDGKIRVMARDMGQGGLPIPSTGGDDWKKDER*
AnnotationID:2242GeneID:2234
AnnotatorUID: diggins
Modification Date/Time: 2005-04-12 20:08:17
Gene Name:xcpX
Confidence Code:1
GeneProduct:general secretion pathway protein K
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(19) Protein secretion/export apparatus
Alternate Gene Product Name:Type II secretory pathway, component PulK
Functional Category Confidence Code:5
COGs:pfam03934,COG3156
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAC83362.1| (AF092918) outer membrane secretion protein X [Pseudomonas
           alcaligenes]
          Length = 317

 Score =  296 bits (757), Expect = 2e-79
 Identities = 171/319 (53%), Positives = 210/319 (65%), Gaps = 14/319 (4%)

>gb|AAM35591.1| (AE011699) type II secretion system protein K [Xanthomonas
           axonopodis pv. citri str. 306]
          Length = 329

 Score =  142 bits (359), Expect = 2e-33
 Identities = 113/323 (34%), Positives = 170/323 (52%), Gaps = 24/323 (7%)






 
Structural Features:
pfam03934, GspK, General secretion pathway protein K.
CD-Length = 306 residues, 100.0% aligned
Score =  331 bits (851), Expect = 5e-92
Identities = 145/313 (46%), Positives = 186/313 (59%), Gaps = 12/313 (3%)

COG3156, PulK, Type II secretory pathway, component PulK 
[Intracellular trafficking and secretion]
CD-Length = 323 residues, 100.0% aligned
Score =  294 bits (755), Expect = 6e-81
Identities = 137/330 (41%), Positives = 183/330 (55%), Gaps = 18/330 (5%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4471




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21617




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32427




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2234_cdd.html


    		




 
URL:

















    ReferenceID:33452




  
Author/Investigator(s):
        de Groot A, Gerritse G, Tommassen J, Lazdunski A, Filloux A.





  
Title:
        Molecular organization of the xcp gene cluster in Pseudomonas putida: absence of an xcpX (gspK) homologue.




  
PubMed:
        9889311


MedLine:
        




    
Source:
Gene. 1999 Jan 8;226(1):35-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9889311

















    ReferenceID:33453




  
Author/Investigator(s):
        Filloux A, Michel G, Bally M.





  
Title:
        GSP-dependent protein secretion in gram-negative bacteria: the Xcp system of Pseudomonas aeruginosa.




  
PubMed:
        9818381


MedLine:
        




    
Source:
FEMS Microbiol Rev. 1998 Sep;22(3):177-98.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9818381

















    ReferenceID:33454




  
Author/Investigator(s):
        Bleves S, Voulhoux R, Michel G, Lazdunski A, Tommassen J, Filloux A.





  
Title:
        The secretion apparatus of Pseudomonas aeruginosa: identification of a fifth pseudopilin, XcpX (GspK family).




  
PubMed:
        9466253


MedLine:
        




    
Source:
Mol Microbiol. 1998 Jan;27(1):31-40.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9466253









           
Homologs By Global Alignment 

           

       



     
Gene ID:2234
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3792
                           PA3097_tr
                           translation of PA3097
                           333
                           96.39
                           98.19
                           0
                           1641.0
     

     
     
116947
                           gb|AAG06485.1|AE004734_3
                           (AE004734) general secretion pathway protein K [Pseudomonas aeruginosa PAO1]
                           333
                           96.39
                           98.19
                           0
                           1641.0
     

     
     
116948
                           emb|CAA44539.1|
                           (X62666) secretion protein XcpX [Pseudomonas aeruginosa]
                           333
                           96.39
                           98.19
                           0
                           1641.0
     

     
     
116949
                           gb|AAC83362.1|
                           (AF092918) outer membrane secretion protein X [Pseudomonas alcaligenes]
                           335
                           51.94
                           64.47
                           20
                           766.0
     

     






      

          
GC ORFID: 45236How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3097Glimmer Score: 

          
Start: 2864269Stop: 2863268

          
Length: 1002


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3097 translation of PA3097Homolog Bit Score 636.0

          
Other Homologs:  t_PA3097 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGAGGCGCGGGCAGAGCGGCGTCGCGCTGATTACCGTGCTGCTGGTGGTGGCGGTGGTGACCATCGTCTGC 
GCCGGGCTGATCATCCGCCAGCAGTTGGCGATACGCAGCAGCGCCAACCAGTTGCACGTGCGCCAGGCCTGG 
CACTATGCGCTGGGCGGCGAACGCCTGGCGGAAGCGGTGCTGCGCCGCGACCTGCGCCAGGGCGGCGAGAAT 
ACCCGCGAGCCGGTGGACCACCTGGGCGAGGCCTGGGCGCGACCGATGACGCCGTTCAAACTGGACGACGGC 
GGCGAGCTGCGGGTGCGGATCGAGGACCCCAGCGGGCGTTTCAACCTCAACGGACTGGTGCGCAAGCGCAAG 
GTCAAGCCGGATTCGGTGAAGCAGTTCCGTCGCCTGCTGGCCACCCTGGGGATGAAGGAAGAGATCGTTCAG 
GGCTTGCCGGACCGGCTGGCCGACTGGCTCGACGCCGACCAGAATCCGCAGGGCGAGCAAGGCGCCGAGGAC 
AACCAGTACCTGCTGGAGGCGCCGGCCTACCGCGCCGCCAACCGCAGTTTCAAGGACGTGTCCGAGCTGCGC 
CTGCTGAAATTGTCGGAAGCCGACTATCGACGCCTGCTGCCGTTCGTCAGCGCCTTGCCCGAGGATGCGCCG 
CTGAACGTGAACACCGCCAGCGTGCCGGTGCTGGCCGCCATGTTCGAGATCGATCCGGGACAGGCGGAAAAC 
ATCGTGGCGGCTCGTGGGCGAGAAGGCTTCCAGGAGAAGACTGAGTTCAGCAAGCACCTGAACGAGTTGGGA 
TCAAAGGCAAGTAACGTCAACTTTGCCGTCGGCACCCGCTACTTCCAGGTGATCAGCGAGGTCAGCCTGGGC 
GACCGCCGGCAGGTGCTGGTGAGTACCTTGCAGCGGGGCAAGGACGGCAAGATACGCGTGATGGCCCGCGAC 
ATGGGGCAGGGCGGCCTGCCCATCCCGTCGACGGGCGGCGACGATTGGAAGAAGGACGAGCGATGA