ViewGene
Locus Name:
GeneID:
Location:

GeneID:2226
TrivialName: PA14_03770
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_03770
PAO1 Ortholog Locus:PA0288
Sequence Length:957
Protein Length:318
Start: 337768
Stop: 338724
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG03677.1|AE004466_11 (AE004466) agmatinase [Pseudomonas aeruginosa PAO1]
Identities = 317/318 (99%), Positives = 317/318 (99%)
Sequence:
ATGTCGAACGACCATCCGCAGCCCCTCGACGCCGCCGAGATTCCACGCTTCGCCGGTATCCCGACCTTCATG
CGCCTGCCGGCGTTCACCGACCCGGCGGCGCTGCAGGTCGGCCTGATCGGCGTGCCCTGGGACGGCGGCACC
ACCAACCGCGCCGGTGCCCGCCATGGCCCGCGCGAGGTGCGCAACCTGTCCAGCCTGATGCGCAAGGTGCAT
CACGTCAGCCGCATTGCGCCCTACGACCTGGTGCGGGTCGGCGACCTCGGTGATGCGCCGGTGAATCCCATC
GACCTGCTCGATTCGCTGCGCCGCATCGAAGGCTTCTATCGCCAGGTCCATGCCGCCGGTACCCTGCCGCTG
TCGGTCGGTGGCGACCACCTGGTGACCCTGCCGATCTTTCGCGCCCTGGGTCGCGAGCGGCCGCTGGGCATG
GTGCATTTCGATGCGCATTCCGATACCAACGACCGCTACTTCGGCGACAACCCCTACACCCACGGCACGCCA
TTCCGCCGGGCCATCGAGGAAGGTCTGCTCGACCCGCTGCGCACGGTGCAGATCGGCATTCGTGGCTCGGTG
TATTCGCCGGACGACGACGCTTTCGCCCGCGAGTGCGGCATCCGCGTGATCCACATGGAAGAGTTCGTCGAA
CTGGGCGTCGAGGCGACCCTGGCCGAAGCCCGGCGGGTGGTGGGCGACGGGCCGACCTATGTGAGTTTCGAC
GTCGACGTGCTCGACCCGGCCTTCGCGCCTGGCACCGGCACCCCGGAGATCGGCGGCATGACCAGCCTCCAG
GCCCAGCAACTGGTCCGTGGCCTGCGCGGGCTGGACCTGGTCGGCGCCGACGTGGTGGAAGTCTCGCCGCCG
TTCGATGTCGGCGGCGCCACGGCGTTGGTGGGGGCGACCATGATGTTCGAACTGCTCTGCCTGCTTGCCGAG
TCGGCCGCGCGAAGCGCCTGA
Translation:
MSNDHPQPLDAAEIPRFAGIPTFMRLPAFTDPAALQVGLIGVPWDGGTTNRAGARHGPREVRNLSSLMRKVH
HVSRIAPYDLVRVGDLGDAPVNPIDLLDSLRRIEGFYRQVHAAGTLPLSVGGDHLVTLPIFRALGRERPLGM
VHFDAHSDTNDRYFGDNPYTHGTPFRRAIEEGLLDPLRTVQIGIRGSVYSPDDDAFARECGIRVIHMEEFVE
LGVEATLAEARRVVGDGPTYVSFDVDVLDPAFAPGTGTPEIGGMTSLQAQQLVRGLRGLDLVGADVVEVSPP
FDVGGATALVGATMMFELLCLLAESAARSA*
AnnotationID:2234GeneID:2226
AnnotatorUID: diggins
Modification Date/Time: 2005-03-24 06:06:04
Gene Name:speB1
Confidence Code:2
GeneProduct:agmatinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0010,pfam00491
Secondary Functional Category(ies):
EC Number:3.5.3.11
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN70097.1|AE016649_5 (AE016791) agmatinase, putative [Pseudomonas putida KT2440]
          Length = 320

 Score =  309 bits (791), Expect = 2e-83
 Identities = 151/305 (49%), Positives = 208/305 (68%), Gaps = 2/305 (0%)

>dbj|BAB51449.1| (AP003005) agmatinase [Mesorhizobium loti]
          Length = 331

 Score =  271 bits (693), Expect = 4e-72
 Identities = 146/307 (47%), Positives = 203/307 (66%), Gaps = 13/307 (4%)








 
Structural Features:
COG0010, SpeB, Arginase/agmatinase/formimionoglutamate hydrolase, arginase family 
[Amino acid transport and metabolism]
CD-Length = 305 residues,  99.7% aligned
Score =  241 bits (616), Expect = 9e-65
Identities = 135/307 (43%), Positives = 184/307 (59%), Gaps = 11/307 (3%)

pfam00491, Arginase, Arginase family. 
CD-Length = 295 residues,  97.6% aligned
Score =  188 bits (478), Expect = 9e-49
Identities = 105/298 (35%), Positives = 151/298 (50%), Gaps = 36/298 (12%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4455




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21601




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33641




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2226_cdd.html


    		




 
URL:

















    ReferenceID:35438




  
Author/Investigator(s):
        Szumanski MB, Boyle SM.





  
Title:
        Analysis and sequence of the speB gene encoding agmatine ureohydrolase, a putrescine biosynthetic enzyme in Escherichia coli.




  
PubMed:
        2153656


MedLine:
        




    
Source:
J Bacteriol. 1990 Feb;172(2):538-47.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=2153656









           
Homologs By Global Alignment 

           

       



     
Gene ID:2226
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3781
                           PA0288_tr
                           translation of PA0288
                           318
                           99.68
                           99.68
                           0
                           1645.0
     

     
     
116613
                           gb|AAG03677.1|AE004466_11
                           (AE004466) agmatinase [Pseudomonas aeruginosa PAO1]
                           318
                           99.68
                           99.68
                           0
                           1645.0
     

     
     
116615
                           gb|AAG04810.1|AE004571_9
                           (AE004571) guanidinobutyrase [Pseudomonas aeruginosa PAO1]
                           322
                           48.13
                           67.08
                           7
                           794.0
     

     
     
3782
                           PA1421_tr
                           translation of PA1421
                           322
                           47.82
                           67.08
                           7
                           790.0
     

     
     
116614
                           gb|AAN70097.1|AE016649_5
                           (AE016791) agmatinase, putative [Pseudomonas putida KT2440]
                           323
                           47.05
                           65.01
                           8
                           789.0
     

     






      

          
GC ORFID: 45228How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0288Glimmer Score: 

          
Start: 6139109Stop: 6140065

          
Length: 957


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0288 translation of PA0288Homolog Bit Score 638.0

          
Other Homologs:  t_PA1421 (ORF: BLASTX 307.0), t_PA0288 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGAACGACCATCCGCAGCCCCTCGACGCCGCCGAGATTCCACGCTTCGCCGGTATCCCGACCTTCATG 
CGCCTGCCGGCGTTCACCGACCCGGCGGCGCTGCAGGTCGGCCTGATCGGCGTGCCCTGGGACGGCGGCACC 
ACCAACCGCGCCGGTGCCCGCCATGGCCCGCGCGAGGTGCGCAACCTGTCCAGCCTGATGCGCAAGGTGCAT 
CACGTCAGCCGCATTGCGCCCTACGACCTGGTGCGGGTCGGCGACCTCGGTGATGCGCCGGTGAATCCCATC 
GACCTGCTCGATTCGCTGCGCCGCATCGAAGGCTTCTATCGCCAGGTCCATGCCGCCGGTACCCTGCCGCTG 
TCGGTCGGTGGCGACCACCTGGTGACCCTGCCGATCTTTCGCGCCCTGGGTCGCGAGCGGCCGCTGGGCATG 
GTGCATTTCGATGCGCATTCCGATACCAACGACCGCTACTTCGGCGACAACCCCTACACCCACGGCACGCCA 
TTCCGCCGGGCCATCGAGGAAGGTCTGCTCGACCCGCTGCGCACGGTGCAGATCGGCATTCGTGGCTCGGTG 
TATTCGCCGGACGACGACGCTTTCGCCCGCGAGTGCGGCATCCGCGTGATCCACATGGAAGAGTTCGTCGAA 
CTGGGCGTCGAGGCGACCCTGGCCGAAGCCCGGCGGGTGGTGGGCGACGGGCCGACCTATGTGAGTTTCGAC 
GTCGACGTGCTCGACCCGGCCTTCGCGCCTGGCACCGGCACCCCGGAGATCGGCGGCATGACCAGCCTCCAG 
GCCCAGCAACTGGTCCGTGGCCTGCGCGGGCTGGACCTGGTCGGCGCCGACGTGGTGGAAGTCTCGCCGCCG 
TTCGATGTCGGCGGCGCCACGGCGTTGGTGGGGGCGACCATGATGTTCGAACTGCTCTGCCTGCTTGCCGAG 
TCGGCCGCGCGAAGCGCCTGA