ViewGene
Locus Name:
GeneID:
Location:

GeneID:2225
TrivialName: PA14_72510
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_72510
PAO1 Ortholog Locus:PA5495
Sequence Length:951
Protein Length:316
Start: 6459838
Stop: 6460788
Strand: +
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08880.1|AE004962_4 (AE004962) homoserine kinase [Pseudomonas aeruginosa PAO1]
Identities = 314/316 (99%), Positives = 315/316 (99%)
Sequence:
ATGTCGGTGTTCACCCCACTCGAACGCTCGACCCTGGAAGCCTTCCTCGCCCCCTACGACCTGGGCCGCCTG
CGGGATTTCCGTGGCATCGCCGAAGGCTCCGAGAACAGCAACTTCTTCGTCAGCCTGGAACACGGCGAGTTC
GTCCTGACCCTGGTCGAGCGCGGGCCGGTGCAGGACCTGCCGTTCTTCATCGAACTGCTCGACGTGCTCCAC
GAGGACGGCCTGCCGGTACCCTACGCCCTGCGCACCCGCGACGGCGAGGCCCTGCGCCGTCTCGAAGGCAAG
CCGGCGCTGCTGCAACCGCGCCTGGCCGGCCGCCACGAGCGGCAGCCGAACGCCCACCACTGCCAGGAAGTG
GGCGATCTGCTCGGCCACCTTCACGCCGCCACCCGCGGGCGCATCCTCGAACGCCCCAGCGACCGTGGCCTG
CCCTGGATGCTGGAACAAGGTGCCAACCTCGCCCCGCGACTACCGGAACAGGCCCGCGCCCTGCTCGCCCCG
GCCCTGGCGGAGATCGCCGCGCTGGATGCCGAGCGCCCTGCGCTGCCGCGCGCCAACCTGCATGCCGACCTG
TTCCGCGACAACGTGCTGTTCGACGGCCCGCACCTGGCCGGCCTGATCGACTTCTACAACGCCTGCTCCGGC
TGGATGCTCTACGACCTGGCGATCACCCTGAACGACTGGTGCTCCAATGCCGACGGCAGCCTGGACCCGGCC
CGCGCCCGCGCCCTGCTCGCCGCCTACGCCAACCGCCGGCCGTTCACCGCGCTGGAAGCCGAGCACTGGCCG
AGCATGCTGCGGGTCGCCTGCGTGCGCTTCTGGCTGTCGCGACTGATCGCCGCCGAGGCGTTCGCCGGACAG
GACGTGCTGATCCATGATCCGGCGGAGTTCGAGATGCGCCTGGCGCAACGGCAGAACGTGGAGATTCATTTG
CCGTTTGCGTTGTAG
Translation:
MSVFTPLERSTLEAFLAPYDLGRLRDFRGIAEGSENSNFFVSLEHGEFVLTLVERGPVQDLPFFIELLDVLH
EDGLPVPYALRTRDGEALRRLEGKPALLQPRLAGRHERQPNAHHCQEVGDLLGHLHAATRGRILERPSDRGL
PWMLEQGANLAPRLPEQARALLAPALAEIAALDAERPALPRANLHADLFRDNVLFDGPHLAGLIDFYNACSG
WMLYDLAITLNDWCSNADGSLDPARARALLAAYANRRPFTALEAEHWPSMLRVACVRFWLSRLIAAEAFAGQ
DVLIHDPAEFEMRLAQRQNVEIHLPFAL*
AnnotationID:2233GeneID:2225
AnnotatorUID: diggins
Modification Date/Time: 2005-03-21 08:08:55
Gene Name:thrB
Confidence Code:1
GeneProduct:homoserine kinase
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(2) Amino acid biosynthesis and metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2334
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65755.1|AE016201_7 (AE016774) homoserine kinase [Pseudomonas putida KT2440]
          Length = 316

 Score =  456 bits (1173), Expect = e-128
 Identities = 223/316 (70%), Positives = 257/316 (81%)

>gb|AAO53891.1| (AE016857) homoserine kinase [Pseudomonas syringae pv. tomato str.
           DC3000]
          Length = 316

 Score =  452 bits (1164), Expect = e-127
 Identities = 224/316 (70%), Positives = 252/316 (79%)








 
Structural Features:
COG2334, COG2334, Putative homoserine kinase type II (protein kinase fold)
CD-Length = 331 residues,  97.9% aligned
Score =  224 bits (573), Expect = 9e-60
Identities = 125/327 (38%), Positives = 154/327 (47%), Gaps = 14/327 (4%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4453




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21599




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:33250




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2225_cdd.html


    		




 
URL:

















    ReferenceID:35598




  
Author/Investigator(s):
        Clepet C, Borne F, Krishnapillai V, Baird C, Patte JC, Cami B.





  
Title:
        Isolation, organization and expression of the Pseudomonas aeruginosa threonine genes.




  
PubMed:
        1333566


MedLine:
        




    
Source:
Mol Microbiol. 1992 Nov;6(21):3109-19.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1333566









           
Homologs By Global Alignment 

           

       



     
Gene ID:2225
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3780
                           PA5495_tr
                           translation of PA5495
                           316
                           99.36
                           99.68
                           0
                           1646.0
     

     
     
116600
                           gb|AAG08880.1|AE004962_4
                           (AE004962) homoserine kinase [Pseudomonas aeruginosa PAO1]
                           316
                           99.36
                           99.68
                           0
                           1646.0
     

     
     
116601
                           emb|CAA46169.1|
                           (X65034) homoserine kinase [Pseudomonas aeruginosa]
                           336
                           73.21
                           74.70
                           40
                           1202.0
     

     
     
116603
                           gb|AAO53891.1|
                           (AE016857) homoserine kinase [Pseudomonas syringae pv. tomato str. DC3000]
                           316
                           70.88
                           79.74
                           0
                           1164.0
     

     
     
116602
                           gb|AAN65755.1|AE016201_7
                           (AE016774) homoserine kinase [Pseudomonas putida KT2440]
                           316
                           70.56
                           81.32
                           0
                           1173.0
     

     






      

          
GC ORFID: 45227How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5495Glimmer Score: 

          
Start: 5723527Stop: 5724477

          
Length: 951


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TAGTruncated Stop: 

          
Homolog: t_PA5495 translation of PA5495Homolog Bit Score 638.0

          
Other Homologs:  psyr_15may02_Scaffold2_revised_gene3186 (ORF: BLASTX 457.0), t_PA5495 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCGGTGTTCACCCCACTCGAACGCTCGACCCTGGAAGCCTTCCTCGCCCCCTACGACCTGGGCCGCCTG 
CGGGATTTCCGTGGCATCGCCGAAGGCTCCGAGAACAGCAACTTCTTCGTCAGCCTGGAACACGGCGAGTTC 
GTCCTGACCCTGGTCGAGCGCGGGCCGGTGCAGGACCTGCCGTTCTTCATCGAACTGCTCGACGTGCTCCAC 
GAGGACGGCCTGCCGGTACCCTACGCCCTGCGCACCCGCGACGGCGAGGCCCTGCGCCGTCTCGAAGGCAAG 
CCGGCGCTGCTGCAACCGCGCCTGGCCGGCCGCCACGAGCGGCAGCCGAACGCCCACCACTGCCAGGAAGTG 
GGCGATCTGCTCGGCCACCTTCACGCCGCCACCCGCGGGCGCATCCTCGAACGCCCCAGCGACCGTGGCCTG 
CCCTGGATGCTGGAACAAGGTGCCAACCTCGCCCCGCGACTACCGGAACAGGCCCGCGCCCTGCTCGCCCCG 
GCCCTGGCGGAGATCGCCGCGCTGGATGCCGAGCGCCCTGCGCTGCCGCGCGCCAACCTGCATGCCGACCTG 
TTCCGCGACAACGTGCTGTTCGACGGCCCGCACCTGGCCGGCCTGATCGACTTCTACAACGCCTGCTCCGGC 
TGGATGCTCTACGACCTGGCGATCACCCTGAACGACTGGTGCTCCAATGCCGACGGCAGCCTGGACCCGGCC 
CGCGCCCGCGCCCTGCTCGCCGCCTACGCCAACCGCCGGCCGTTCACCGCGCTGGAAGCCGAGCACTGGCCG 
AGCATGCTGCGGGTCGCCTGCGTGCGCTTCTGGCTGTCGCGACTGATCGCCGCCGAGGCGTTCGCCGGACAG 
GACGTGCTGATCCATGATCCGGCGGAGTTCGAGATGCGCCTGGCGCAACGGCAGAACGTGGAGATTCATTTG 
CCGTTTGCGTTGTAG