ViewGene

Locus Name:
GeneID:
Location:

GeneID: 222

TrivialName: PA14_39010

AnnotatorUID:

Modification Date/Time: 2006-04-06 13:01:23

LocusName: PA14_39010

PAO1 Ortholog Locus: PA1973

Sequence Length: 2328

Protein Length: 775

Start: 3478818

Stop: 3476491

Strand: -

Type:

ChromosomeID: 2

Status: ACTIVE

Frame Discrepancy: FALSE

Missense Discrepancy: FALSE

Comments:
Homology: gb|AAG05361.1|AE004623_11 (AE004623) pyrroloquinoline quinone biosynthesis protein F [Pseudomonas aeruginosa PAO1] Identities = 755/775 (97%), Positives = 763/775 (98%)

Sequence:

ATGGATCGCCACGCCCAACACCACCCCAGAAGCCGCGCCTGCGTGCTGCCCAACGGCCTGCGCCTGCACCTG
GCGCATGATCCGGCGGCATCGCGCGCCGCTGCCTGGCTGCGGGTGGCGGCGGGCAGCCACGACGAGCCGAGC
GCGCATCCCGGCCTGGCCCACTTCCTCGAGCACCTGTCGTTTCTCGGTGGCGCGGCGTTTCCCGGCGACGAG
CGCCTGATGCCGTGGTTGCAGGTGTGCGGCGGCCAGGTCAACGCCAGCACCCGGGGCAGGACCACCGACTAT
TTCTTCGAGGTGACGGCGGAGCACCTTGGCGCCGGTCTGGCACGGCTGATCGACATGCTCGCCCGCCCCTTG
CTGGACATCGATGCGCAACGGCGCGAGCGCGAAGTGCTCGAGGCCGAATACCTGGCGCGCTCGGCCGACGAG
CAGACCCAGATCGACGCGGCGCTGGCCCTTGGCCTGCCCGCCGGACACCCCCTGCGGCGTTTCGCTGCCGGG
CGCCGCGACAGCCTGGCCCTGGAGAACGATGCGTTCCAGCGGGCCCTGCGCGAATTCCATGCCGCCCACTAT
CACGCCGGCAATTGCCAGCTATGGCTGCAAGGGCCGCAGACACTGGACGAGCTGGAGAGGCTGGCGCAGCGT
GCCTGCGCCGACCTGCCGGGGCGCGCGCCAGGCGCGAGTCCGCCGCCGCCGCCGTTGCTGCCCTTCGCCGGC
GAAGCGCTGGCGCTGCGCCTGCCGGGACCGCCGCGGCTGGTGCTGGGCTTTGCCCTCGACGCTTTGCGGGGG
GCCGACGAACAGACCCTGCTGGCGTTCGCCGAATTGCTCGGCGATCGCTCGCCGGGTGGGTTGCTGGCGGCA
CTCGGCGAACAGGGCCTGGGCGAATCGGTGGCGCTGCGGGTGGTTCATCGGGACGCGCGGCAGGCGCTCCTG
GCGTTGACCTTCGAACTGTTCGACGGCAGCGCGGCGGCGGCGCTGGAGGCGGCCTTTTTCGACTGGCTGGGC
GCCCTGCGCGACGATGCCGCCAGCTTGCTGGCGGCGCGCCAGCCGTTGCTGGCGGAGCCCACTGCGCCGCTG
GAGCGACTACGCCAGCGCGTTCTCGGCCTGCCGGCGGAGATTCGCCCGGCCTGTCTGGACGCGCTGCAAGCC
GACCGCTGCCTGCGGCTGCACCTGGACAGCGAACTCGACGGCGCCGAAGCGCGCTGGTCGGCGGGCTTCCGC
CTGAGCGTGGCGCCGGTTGCCGCAGCGCCGCCGCTGACCGCGCAGCGGCATGCCTGGCGCTTCGAACTGCCG
TTGCCGCCCAGTGCCGCCGCCGAGGGCGCGCTGTTCCTGCGCTGGCGCTTTCCCGGTGTTCCCGCACGGTCA
CGCTTCCTGGCGCTGCGCCAGGCGTTGCGTCCGCTGTGCGGCCAGGCGCGCCTGGGGGGAGTGGAGATGGGC
CTGGAGGCGCTCGGCGAAGACTGGTCGCTGAGCCTGCTCGGTCCGCGCGATCGCCTCGAGGCCGCCGTGCGC
ACGGCCCTGGCCCGGCTCCTCGCGGCGCCGCCCGACTGGCGCGCGAGCGGCGAGCGTCTGTCCTCCGCCGAG
CGGCGCCGCAGCGCCACTGGCCTGCCGATCCGGCAGTTGCTGGACGCCTTGCCCGGCCTACTCGGCGAGCCG
CTGGCGGAGGTCGACGACTGGCGGCGGACGCGCTGGGACGCCTTGGTCATGCAGGCCGCGATGCCTGATCCA
CGATGGATGCCCGGCCAGGCTGCCGGCGAGCGCCTGGCGCCTCTGCCGCCGAGGCCCGGACGGCATCGCCGC
GAACTGGCCGTGGACGGCGAGTCGGCGTTGCTGTTGTTCTGTCCGCTGCCCTCGCCGGAGGTGCCGATGGAG
GCGGCCTGGCGCTTGCTGGCGCGCCTGCACGAACCGGCCTTCCAGCGGCGCCTGCGCGACGAGCTGCAACTG
GGCTACGCGCTGTTCTGCGGCTTCCGCGAGGTCGGCGCCCGGCGCGGCCTGCTGTTCGCCGCGCAATCTCCG
CGTGCCTGCCCGGAACGCCTGCTGGAGCATATGGAGACCTTCCTCCAGCGCTCCGTCGAGGCGCTCGCGCAA
CTGCCGGCGCAACGCCTGGCCGGCCTGCGCGAAGCCCTCGCCGACGACCTGCGCCGAGCGCCGGGTAGCTTC
GCCGAGCGAGCACGGCGCGCCTGGGCGGAGCACCTTGGCGGTGGCGCCGGTCGCTCCCGGCTGCTCGCCGAG
GCCGCCCTCGGCCTGAGCGGCGACGACCTGCTCGCGGCCCAGGCCCGGTTGCTGGAGGCGCGCGGCGGCTGG
TGGGTGCTTTCCAGCCGGCGCTGA

Translation:

MDRHAQHHPRSRACVLPNGLRLHLAHDPAASRAAAWLRVAAGSHDEPSAHPGLAHFLEHLSFLGGAAFPGDE
RLMPWLQVCGGQVNASTRGRTTDYFFEVTAEHLGAGLARLIDMLARPLLDIDAQRREREVLEAEYLARSADE
QTQIDAALALGLPAGHPLRRFAAGRRDSLALENDAFQRALREFHAAHYHAGNCQLWLQGPQTLDELERLAQR
ACADLPGRAPGASPPPPPLLPFAGEALALRLPGPPRLVLGFALDALRGADEQTLLAFAELLGDRSPGGLLAA
LGEQGLGESVALRVVHRDARQALLALTFELFDGSAAAALEAAFFDWLGALRDDAASLLAARQPLLAEPTAPL
ERLRQRVLGLPAEIRPACLDALQADRCLRLHLDSELDGAEARWSAGFRLSVAPVAAAPPLTAQRHAWRFELP
LPPSAAAEGALFLRWRFPGVPARSRFLALRQALRPLCGQARLGGVEMGLEALGEDWSLSLLGPRDRLEAAVR
TALARLLAAPPDWRASGERLSSAERRRSATGLPIRQLLDALPGLLGEPLAEVDDWRRTRWDALVMQAAMPDP
RWMPGQAAGERLAPLPPRPGRHRRELAVDGESALLLFCPLPSPEVPMEAAWRLLARLHEPAFQRRLRDELQL
GYALFCGFREVGARRGLLFAAQSPRACPERLLEHMETFLQRSVEALAQLPAQRLAGLREALADDLRRAPGSF
AERARRAWAEHLGGGAGRSRLLAEAALGLSGDDLLAAQARLLEARGGWWVLSSRR*

AnnotationID:232 GeneID:222

AnnotatorUID: diggins

Modification Date/Time: 2005-04-28 16:04:34

Gene Name: pqqF

Confidence Code: 2

GeneProduct: pyrroloquinoline quinone biosynthesis protein F

Cell Localization: ()

Synonyms:

Cell Localization Confidence Code: 5

MolecularFunction:

Functional Category: (4) Biosynthesis of cofactors, prosthetic groups and carriers

Alternate Gene Product Name: putative periplasmic Zn-dependent peptidase

Functional Category Confidence Code: 5

COGs: COG1025

Secondary Functional Category(ies): posttranslational modification, protein turnover, chaperones

EC Number: 3.4.99.-

Status: ACTIVE

Pathway:

Homology:

>gb|AAO54057.1| (AE016857) coenzyme PQQ synthesis protein F [Pseudomonas syringae
           pv. tomato str. DC3000]
          Length = 779

 Score =  351 bits (901), Expect = 9e-96
 Identities = 277/791 (35%), Positives = 373/791 (47%), Gaps = 66/791 (8%)

>emb|CAA60730.1| (X87299) pyrroloquinoline quinone F biosynthesis [Pseudomonas
           fluorescens]
          Length = 829

 Score =  345 bits (884), Expect = 8e-94
 Identities = 291/816 (35%), Positives = 379/816 (46%), Gaps = 66/816 (8%)

Structural Features:

COG1025, Ptr, Secreted/periplasmic Zn-dependent peptidases, insulinase-like 
[Posttranslational modification, protein turnover, chaperones]
CD-Length = 937 residues,  95.3% aligned
Score =  705 bits (1821), Expect = 0.0
Identities = 244/895 (27%), Positives = 349/895 (38%), Gaps = 122/895 (13%)

Genomic Context:

Comment:

ReferenceID:447

Author/Investigator(s): GenNotator

Title:

PubMed:

MedLine:

Source:

Reference Type: BLASTP

Data:

URL:

ReferenceID:17589

Author/Investigator(s): GenNotator

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: RPSBLAST

Data:

URL:

ReferenceID:32751

Author/Investigator(s):

Title: CDD Search

PubMed:

MedLine:

Source:

Reference Type: Misc (text/plain)

Data: 222_cdd.html

URL:

ReferenceID:34363

Author/Investigator(s): Meulenberg JJ, Sellink E, Riegman NH, Postma PW.

Title: Nucleotide sequence and structure of the Klebsiella pneumoniae pqq operon.

PubMed: 1313537

MedLine:

Source:

Mol Gen Genet. 1992 Mar;232(2):284-94.

Reference Type: Journal

Data:

URL:	`http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1313537`

ReferenceID:34364

Author/Investigator(s): Springer AL, Ramamoorthi R, Lidstrom ME.

Title: Characterization and nucleotide sequence of pqqE and pqqF in Methylobacterium extorquens AM1.

PubMed: 8606199

MedLine:

Source:

J Bacteriol. 1996 Apr;178(7):2154-7.

Reference Type: Journal

Data:

URL:	`http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8606199`

Homologs By Global Alignment

Gene ID:222

Identity:

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

HomologID Accession Description Length PctIdentity PctSimilarity Gaps Score

388 PA1973_tr translation of PA1973 775 97.41 98.45 0 3893.0

22471 gb|AAG05361.1|AE004623_11 (AE004623) pyrroloquinoline quinone biosynthesis protein F [Pseudomonas aeruginosa PAO1] 775 97.41 98.45 0 3893.0

GC ORFID: `43224`	How Found: `BLASTX`
GC_TrimmedSeqID: `69`	Blast Result ID
Subject Sequence Name: `t_PA1973`	Glimmer Score:
Start: `1475093`	Stop: `1477420`
Length: `2328`
Start Codon: `ATG`	Truncated Start:
Stop Codon: `TGA`	Truncated Stop:
Homolog: `t_PA1973 translation of PA1973`	Homolog Bit Score `1504.0`
Other Homologs: `t_PA1973 (ORF: GLIMMER)`
GC ORF Sequence
ATGGATCGCCACGCCCAACACCACCCCAGAAGCCGCGCCTGCGTGCTGCCCAACGGCCTGCGCCTGCACCTG GCGCATGATCCGGCGGCATCGCGCGCCGCTGCCTGGCTGCGGGTGGCGGCGGGCAGCCACGACGAGCCGAGC GCGCATCCCGGCCTGGCCCACTTCCTCGAGCACCTGTCGTTTCTCGGTGGCGCGGCGTTTCCCGGCGACGAG CGCCTGATGCCGTGGTTGCAGGTGTGCGGCGGCCAGGTCAACGCCAGCACCCGGGGCAGGACCACCGACTAT TTCTTCGAGGTGACGGCGGAGCACCTTGGCGCCGGTCTGGCACGGCTGATCGACATGCTCGCCCGCCCCTTG CTGGACATCGATGCGCAACGGCGCGAGCGCGAAGTGCTCGAGGCCGAATACCTGGCGCGCTCGGCCGACGAG CAGACCCAGATCGACGCGGCGCTGGCCCTTGGCCTGCCCGCCGGACACCCCCTGCGGCGTTTCGCTGCCGGG CGCCGCGACAGCCTGGCCCTGGAGAACGATGCGTTCCAGCGGGCCCTGCGCGAATTCCATGCCGCCCACTAT CACGCCGGCAATTGCCAGCTATGGCTGCAAGGGCCGCAGACACTGGACGAGCTGGAGAGGCTGGCGCAGCGT GCCTGCGCCGACCTGCCGGGGCGCGCGCCAGGCGCGAGTCCGCCGCCGCCGCCGTTGCTGCCCTTCGCCGGC GAAGCGCTGGCGCTGCGCCTGCCGGGACCGCCGCGGCTGGTGCTGGGCTTTGCCCTCGACGCTTTGCGGGGG GCCGACGAACAGACCCTGCTGGCGTTCGCCGAATTGCTCGGCGATCGCTCGCCGGGTGGGTTGCTGGCGGCA CTCGGCGAACAGGGCCTGGGCGAATCGGTGGCGCTGCGGGTGGTTCATCGGGACGCGCGGCAGGCGCTCCTG GCGTTGACCTTCGAACTGTTCGACGGCAGCGCGGCGGCGGCGCTGGAGGCGGCCTTTTTCGACTGGCTGGGC GCCCTGCGCGACGATGCCGCCAGCTTGCTGGCGGCGCGCCAGCCGTTGCTGGCGGAGCCCACTGCGCCGCTG GAGCGACTACGCCAGCGCGTTCTCGGCCTGCCGGCGGAGATTCGCCCGGCCTGTCTGGACGCGCTGCAAGCC GACCGCTGCCTGCGGCTGCACCTGGACAGCGAACTCGACGGCGCCGAAGCGCGCTGGTCGGCGGGCTTCCGC CTGAGCGTGGCGCCGGTTGCCGCAGCGCCGCCGCTGACCGCGCAGCGGCATGCCTGGCGCTTCGAACTGCCG TTGCCGCCCAGTGCCGCCGCCGAGGGCGCGCTGTTCCTGCGCTGGCGCTTTCCCGGTGTTCCCGCACGGTCA CGCTTCCTGGCGCTGCGCCAGGCGTTGCGTCCGCTGTGCGGCCAGGCGCGCCTGGGGGGAGTGGAGATGGGC CTGGAGGCGCTCGGCGAAGACTGGTCGCTGAGCCTGCTCGGTCCGCGCGATCGCCTCGAGGCCGCCGTGCGC ACGGCCCTGGCCCGGCTCCTCGCGGCGCCGCCCGACTGGCGCGCGAGCGGCGAGCGTCTGTCCTCCGCCGAG CGGCGCCGCAGCGCCACTGGCCTGCCGATCCGGCAGTTGCTGGACGCCTTGCCCGGCCTACTCGGCGAGCCG CTGGCGGAGGTCGACGACTGGCGGCGGACGCGCTGGGACGCCTTGGTCATGCAGGCCGCGATGCCTGATCCA CGATGGATGCCCGGCCAGGCTGCCGGCGAGCGCCTGGCGCCTCTGCCGCCGAGGCCCGGACGGCATCGCCGC GAACTGGCCGTGGACGGCGAGTCGGCGTTGCTGTTGTTCTGTCCGCTGCCCTCGCCGGAGGTGCCGATGGAG GCGGCCTGGCGCTTGCTGGCGCGCCTGCACGAACCGGCCTTCCAGCGGCGCCTGCGCGACGAGCTGCAACTG GGCTACGCGCTGTTCTGCGGCTTCCGCGAGGTCGGCGCCCGGCGCGGCCTGCTGTTCGCCGCGCAATCTCCG CGTGCCTGCCCGGAACGCCTGCTGGAGCATATGGAGACCTTCCTCCAGCGCTCCGTCGAGGCGCTCGCGCAA CTGCCGGCGCAACGCCTGGCCGGCCTGCGCGAAGCCCTCGCCGACGACCTGCGCCGAGCGCCGGGTAGCTTC GCCGAGCGAGCACGGCGCGCCTGGGCGGAGCACCTTGGCGGTGGCGCCGGTCGCTCCCGGCTGCTCGCCGAG GCCGCCCTCGGCCTGAGCGGCGACGACCTGCTCGCGGCCCAGGCCCGGTTGCTGGAGGCGCGCGGCGGCTGG TGGGTGCTTTCCAGCCGGCGCTGA