ViewGene
Locus Name:
GeneID:
Location:

GeneID:2218
TrivialName: PA14_71140
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_71140
PAO1 Ortholog Locus:PA5386
Sequence Length:966
Protein Length:321
Start: 6337766
Stop: 6336801
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG08771.1|AE004951_5 (AE004951) probable 3-hydroxyacyl-CoA dehydrogenase [Pseudomonas aeruginosa PAO1]
Identities = 321/321 (100%), Positives = 321/321 (100%)
Sequence:
ATGTCCTTCGTCACCGAGATCAAGACCTTCGCCGCCCTCGGTAGCGGCGTCATCGGCAGCGGCTGGATCGCC
CGCGCCCTGGCCCACGGCCTCGACGTCGTCGCCTGGGATCCGGCGCCGGGCGCCGAAGCGGCGTTGCGCGCG
CGCGTCGCCAATGCCTGGCCGGCCTTGCGGAAACAGGGCCTGGCGCCCGGCGCGGCGCAGGAACGCCTGCGC
TTCGTCGCGAGCATCGAGGAATGCGTGGGCGACGCCGATTTCATCCAGGAGAGCGCCCCCGAGCGGCTCGAC
CTGAAGCTCGACCTGCACGCCAGGATCAGCGCCGCGGCGCGGCCCGACGTGCTGATCGGTTCCTCCACCTCG
GGCCTGCTGCCCAGCGAGTTCTACGCCGAGGCGAGCCACCCCGAGCGCTGCCTGGTCGGCCACCCCTTCAAT
CCGGTCTACCTGCTGCCGCTGGTGGAGGTGGTCGGCGGCGAACGCACCGCTGCCGAGGCCGTGCGGGCGGCG
ATGCGGGTCTACGAGTCGCTGGGCATGCGTCCGCTGCACGTACGCAAGGAAGTGCCCGGCTTCATCGCCGAC
CGCTTGCTCGAGGCGCTCTGGCGCGAGGCGCTGCACCTGGTCAACGACGGCGTGGCCACGACCGGCGAGATC
GACGATGCGATCCGCTTCGGCGCCGGCCTGCGCTGGTCGTTCATGGGCACCTTCCTGACCTACACCCTGGCC
GGTGGCAACGCCGGCATGCGCCACTTCATGGCGCAGTTCGGCCCGGCGCTGCAATTGCCCTGGACCTACCTG
CCGGCGCCGGAACTCACCGAAGCCCTGATCGACCGGGTGGTGGAGGGCACCGCCGAGCAGCAGGGCGCGCGC
AGCATCGCCGAGCTCGAGCGCTACCGCGACGACTGCCTGCTGGCGGTGCTCGGGGCGATCAGGGAAACCAAG
GCCCGGCACGGCTTCGCCTTCGCCGAGTGA
Translation:
MSFVTEIKTFAALGSGVIGSGWIARALAHGLDVVAWDPAPGAEAALRARVANAWPALRKQGLAPGAAQERLR
FVASIEECVGDADFIQESAPERLDLKLDLHARISAAARPDVLIGSSTSGLLPSEFYAEASHPERCLVGHPFN
PVYLLPLVEVVGGERTAAEAVRAAMRVYESLGMRPLHVRKEVPGFIADRLLEALWREALHLVNDGVATTGEI
DDAIRFGAGLRWSFMGTFLTYTLAGGNAGMRHFMAQFGPALQLPWTYLPAPELTEALIDRVVEGTAEQQGAR
SIAELERYRDDCLLAVLGAIRETKARHGFAFAE*
AnnotationID:2226GeneID:2218
AnnotatorUID: diggins
Modification Date/Time: 2005-03-17 08:08:55
Gene Name:
Confidence Code:3
GeneProduct:putative 3-hydroxyacyl-CoA dehydrogenase
Cell Localization:()
Synonyms:PA5386
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG1250,pfam02737
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAN65933.1|AE016221_5 (AE016775) 3-hydroxyacyl-CoA dehydrogenase family protein
           [Pseudomonas putida KT2440]
          Length = 321

 Score =  555 bits (1429), Expect = e-157
 Identities = 268/321 (83%), Positives = 297/321 (92%)

dbj|BAC12953.1| (AP004596) 3-hydroxyacyl-CoA dehydrogenase [Oceanobacillus
           iheyensis HTE831]
          Length = 307

 Score =  341 bits (875), Expect = 3e-93
 Identities = 167/304 (54%), Positives = 218/304 (71%)






 
Structural Features:
COG1250, FadB, 3-hydroxyacyl-CoA dehydrogenase [Lipid metabolism]
CD-Length = 307 residues,  95.4% aligned
Score =  224 bits (573), Expect = 1e-59
Identities = 106/299 (35%), Positives = 144/299 (48%), Gaps = 10/299 (3%)

pfam02737, 3HCDH_N, 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain.
CD-Length = 185 residues,  97.3% aligned
Score =  118 bits (297), Expect = 9e-28
Identities = 59/181 (32%), Positives = 91/181 (50%), Gaps = 5/181 (2%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4439




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21585




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34708




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2218_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2218
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3768
                           PA5386_tr
                           translation of PA5386
                           321
                           99.99
                           99.99
                           0
                           1647.0
     

     
     
115922
                           gb|AAG08771.1|AE004951_5
                           (AE004951) probable 3-hydroxyacyl-CoA dehydrogenase [Pseudomonas aeruginosa PAO1]
                           321
                           99.99
                           99.99
                           0
                           1647.0
     

     
     
115923
                           gb|AAN65933.1|AE016221_5
                           (AE016775) 3-hydroxyacyl-CoA dehydrogenase family protein [Pseudomonas putida KT2440]
                           321
                           83.48
                           92.52
                           0
                           1429.0
     

     
     
115924
                           dbj|BAC12953.1|
                           (AP004596) 3-hydroxyacyl-CoA dehydrogenase [Oceanobacillus iheyensis HTE831]
                           321
                           52.02
                           67.91
                           14
                           869.0
     

     
     
115925
                           emb|CAC37899.1|
                           (AL939127) putative 3-hydroxyacyl-CoA dehydrogenase [Streptomyces coelicolor A3(2)]
                           325
                           51.69
                           63.69
                           11
                           803.0
     

     






      

          
GC ORFID: 45220How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA5386Glimmer Score: 

          
Start: 5601455Stop: 5600490

          
Length: 966


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA5386 translation of PA5386Homolog Bit Score 639.0

          
Other Homologs:  psyr_15may02_Scaffold7_revised_gene7151 (ORF: BLASTX 544.0), t_PA5386 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCTTCGTCACCGAGATCAAGACCTTCGCCGCCCTCGGTAGCGGCGTCATCGGCAGCGGCTGGATCGCC 
CGCGCCCTGGCCCACGGCCTCGACGTCGTCGCCTGGGATCCGGCGCCGGGCGCCGAAGCGGCGTTGCGCGCG 
CGCGTCGCCAATGCCTGGCCGGCCTTGCGGAAACAGGGCCTGGCGCCCGGCGCGGCGCAGGAACGCCTGCGC 
TTCGTCGCGAGCATCGAGGAATGCGTGGGCGACGCCGATTTCATCCAGGAGAGCGCCCCCGAGCGGCTCGAC 
CTGAAGCTCGACCTGCACGCCAGGATCAGCGCCGCGGCGCGGCCCGACGTGCTGATCGGTTCCTCCACCTCG 
GGCCTGCTGCCCAGCGAGTTCTACGCCGAGGCGAGCCACCCCGAGCGCTGCCTGGTCGGCCACCCCTTCAAT 
CCGGTCTACCTGCTGCCGCTGGTGGAGGTGGTCGGCGGCGAACGCACCGCTGCCGAGGCCGTGCGGGCGGCG 
ATGCGGGTCTACGAGTCGCTGGGCATGCGTCCGCTGCACGTACGCAAGGAAGTGCCCGGCTTCATCGCCGAC 
CGCTTGCTCGAGGCGCTCTGGCGCGAGGCGCTGCACCTGGTCAACGACGGCGTGGCCACGACCGGCGAGATC 
GACGATGCGATCCGCTTCGGCGCCGGCCTGCGCTGGTCGTTCATGGGCACCTTCCTGACCTACACCCTGGCC 
GGTGGCAACGCCGGCATGCGCCACTTCATGGCGCAGTTCGGCCCGGCGCTGCAATTGCCCTGGACCTACCTG 
CCGGCGCCGGAACTCACCGAAGCCCTGATCGACCGGGTGGTGGAGGGCACCGCCGAGCAGCAGGGCGCGCGC 
AGCATCGCCGAGCTCGAGCGCTACCGCGACGACTGCCTGCTGGCGGTGCTCGGGGCGATCAGGGAAACCAAG 
GCCCGGCACGGCTTCGCCTTCGCCGAGTGA