ViewGene
Locus Name:
GeneID:
Location:

GeneID:2209
TrivialName: PA14_57190
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_57190
PAO1 Ortholog Locus:PA4400
Sequence Length:948
Protein Length:315
Start: 5095250
Stop: 5094303
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG07788.1|AE004855_10 (AE004855) probable pyrophosphohydrolase [Pseudomonas aeruginosa PAO1]
Identities = 313/315 (99%), Positives = 314/315 (99%)
Sequence:
GTGAAACGAGTACATGTCGCCGCGGCCGTGATTCGTGGCTCCGATGGCCGGGTGCTGATCGCCCGGCGGCCG
GAAGACAAGCATCAGGGCGGCCTGTGGGAGTTTCCCGGGGGCAAGGTGGAGGACGGCGAGCCGGTGCGCGCG
GCGTTGGCCCGTGAGCTGGAAGAGGAACTGGGCATCCGTGTCGAGCGGGCCCGGCCGCTGATCCAGGTTCGC
CACGACTATGCCGACAAGCACGTCCTGCTCGATGTCTGGGAGGTCGACGGGTTTTCCGGCGAAGCCCACGGC
GTCGAGGGCCAACCGCTGGCCTGGGTGGAGCCGCGCGAGCTGGCGGACTACGAGTTCCCCGCCGCCAACGCG
CCGATCGTCCAGGCCGCGCGCCTGCCGGCGCACTATCTGATCACCCCGGACGGCCTGGAGCCGGGCGAGCTG
ATCAGCGGCGTACGCAAGGCGGTGGAGGCGGGCATCCGCCTCATCCAGTTGCGCGCGCCGAACATGTTCAGT
CCCGAATACCGCGATCTCGCCATTGATATCCAGGGACTCTGCGCCGGCAAGGCGCAACTGATGCTGAAAGGC
CCGCTGGAGTGGCTGGGCGACTTCCCGGCTGCCGGTTGGCACCTGACCTCCGCTCAACTGCGCAAATACGCC
AGCGCCGGTCGGCCGTTCCCCGAAGGGCGCCTGCTGGCCGCCTCCTGCCACGACGCGGAGGAACTGGCCCTG
GCCGCCTCGATGGGAGTGGAGTTCGTCACCCTTTCGCCGGTACAGCCGACCGAGAGCCATCCCGGCGAGCCG
GCGCTGGGGTGGGACAAGGCCGCCGAACTGATCGCCGGCTTCAACCAGCCGGTCTACCTGCTGGGTGGCGTC
GGTCCGCAGCAAGCCGAGCAGGCTTGGGAGCATGGAGCCCAGGGCGTGGCGGGTATCCGTGCGTTCTGGCCG
GGCGGCCTTTGA
Translation:
MKRVHVAAAVIRGSDGRVLIARRPEDKHQGGLWEFPGGKVEDGEPVRAALARELEEELGIRVERARPLIQVR
HDYADKHVLLDVWEVDGFSGEAHGVEGQPLAWVEPRELADYEFPAANAPIVQAARLPAHYLITPDGLEPGEL
ISGVRKAVEAGIRLIQLRAPNMFSPEYRDLAIDIQGLCAGKAQLMLKGPLEWLGDFPAAGWHLTSAQLRKYA
SAGRPFPEGRLLAASCHDAEELALAASMGVEFVTLSPVQPTESHPGEPALGWDKAAELIAGFNQPVYLLGGV
GPQQAEQAWEHGAQGVAGIRAFWPGGL*
AnnotationID:2217GeneID:2209
AnnotatorUID: danlee
Modification Date/Time: 2006-01-31 15:03:26
Gene Name:
Confidence Code:2
GeneProduct:putative pyrophosphohydrolase
Cell Localization:()
Synonyms:PA4400, mutT
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(11) DNA replication, recombination, modification and repair
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0494, COG0352
Secondary Functional Category(ies):
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO57846.1| (AE016871) mutT/nudix family protein [Pseudomonas syringae pv.
           tomato str. DC3000]
          Length = 316

 Score =  487 bits (1253), Expect = e-137
 Identities = 234/312 (75%), Positives = 262/312 (83%)

>gb|AAN66971.1|AE016325_7 (AE016779) MutT/nudix family protein/thiamine-phosphate
           pyrophosphorylase, putative [Pseudomonas putida KT2440]
          Length = 314

 Score =  484 bits (1245), Expect = e-136
 Identities = 233/312 (74%), Positives = 262/312 (83%)







 
Structural Features:
N-Terminal Region:
COG0494, MutT, NTP pyrophosphohydrolases including oxidative damage repair enzymes [DNA replication, 
recombination, and repair / General function prediction only].
             CD-Length = 161 residues,  78.3% aligned
             Score = 40.4 bits (92), Expect = 3e-04

C-Terminal Region:
COG0352, ThiE, Thiamine monophosphate synthase [Coenzyme metabolism].
             CD-Length = 211 residues,  89.6% aligned
             Score =  147 bits (372), Expect = 2e-36






   
Genomic Context:






   
Comment:
The PAO1 and PA14 gene appears to be bifunctional.
The N-terminal part of the protein has been shown to be both necessary and sufficient 
for the 8-oxodGTP hydrolase activity of the E. coli mutT gene.


















    ReferenceID:14541




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21567




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:27729




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2209_cdd.html


    		




 
URL:

















    ReferenceID:38308




  
Author/Investigator(s):
        Oliver A, Sanchez JM, Blazquez J.





  
Title:
        Characterization of the GO system of Pseudomonas aeruginosa.




  
PubMed:
        12445642


MedLine:
        




    
Source:
FEMS Microbiol Lett. 2002 Nov 19;217(1):31-5.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12445642









           
Homologs By Global Alignment 

           

       



     
Gene ID:2209
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3755
                           PA4400_tr
                           translation of PA4400
                           315
                           99.36
                           99.68
                           0
                           1651.0
     

     
     
115783
                           gb|AAG07788.1|AE004855_10
                           (AE004855) probable pyrophosphohydrolase [Pseudomonas aeruginosa PAO1]
                           315
                           99.04
                           99.68
                           0
                           1648.0
     

     
     
115784
                           gb|AAO57846.1|
                           (AE016871) mutT/nudix family protein [Pseudomonas syringae pv. tomato str. DC3000]
                           316
                           73.73
                           83.22
                           1
                           1248.0
     

     
     
115785
                           gb|AAN66971.1|AE016325_7
                           (AE016779) MutT/nudix family protein/thiamine-phosphate pyrophosphorylase, putative [Pseudomonas putida KT2440]
                           315
                           73.65
                           83.17
                           1
                           1236.0
     

     
     
115786
                           gb|AAM40051.1|
                           (AE012173) bifunctional DGTP-pyrophosphohydrolase/thiamine phosphate synthase [Xanthomonas campestris pv. campestris str. ATCC 33913]
                           323
                           40.55
                           52.32
                           16
                           579.0
     

     






      

          
GC ORFID: 45211How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA4400Glimmer Score: 

          
Start: 4358926Stop: 4357979

          
Length: 948


          
Start Codon: GTGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA4400 translation of PA4400Homolog Bit Score 640.0

          
Other Homologs:  psyr_15may02_Scaffold1_revised_gene1139 (ORF: BLASTX 489.0), t_PA4400 (ORF: GLIMMER)

          
GC ORF Sequence

           
GTGAAACGAGTACATGTCGCCGCGGCCGTGATTCGTGGCTCCGATGGCCGGGTGCTGATCGCCCGGCGGCCG 
GAAGACAAGCATCAGGGCGGCCTGTGGGAGTTTCCCGGGGGCAAGGTGGAGGACGGCGAGCCGGTGCGCGCG 
GCGTTGGCCCGTGAGCTGGAAGAGGAACTGGGCATCCGTGTCGAGCGGGCCCGGCCGCTGATCCAGGTTCGC 
CACGACTATGCCGACAAGCACGTCCTGCTCGATGTCTGGGAGGTCGACGGGTTTTCCGGCGAAGCCCACGGC 
GTCGAGGGCCAACCGCTGGCCTGGGTGGAGCCGCGCGAGCTGGCGGACTACGAGTTCCCCGCCGCCAACGCG 
CCGATCGTCCAGGCCGCGCGCCTGCCGGCGCACTATCTGATCACCCCGGACGGCCTGGAGCCGGGCGAGCTG 
ATCAGCGGCGTACGCAAGGCGGTGGAGGCGGGCATCCGCCTCATCCAGTTGCGCGCGCCGAACATGTTCAGT 
CCCGAATACCGCGATCTCGCCATTGATATCCAGGGACTCTGCGCCGGCAAGGCGCAACTGATGCTGAAAGGC 
CCGCTGGAGTGGCTGGGCGACTTCCCGGCTGCCGGTTGGCACCTGACCTCCGCTCAACTGCGCAAATACGCC 
AGCGCCGGTCGGCCGTTCCCCGAAGGGCGCCTGCTGGCCGCCTCCTGCCACGACGCGGAGGAACTGGCCCTG 
GCCGCCTCGATGGGAGTGGAGTTCGTCACCCTTTCGCCGGTACAGCCGACCGAGAGCCATCCCGGCGAGCCG 
GCGCTGGGGTGGGACAAGGCCGCCGAACTGATCGCCGGCTTCAACCAGCCGGTCTACCTGCTGGGTGGCGTC 
GGTCCGCAGCAAGCCGAGCAGGCTTGGGAGCATGGAGCCCAGGGCGTGGCGGGTATCCGTGCGTTCTGGCCG 
GGCGGCCTTTGA