ViewGene
Locus Name:
GeneID:
Location:

GeneID:2203
TrivialName: PA14_08460
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_08460
PAO1 Ortholog Locus:PA0660
Sequence Length:969
Protein Length:322
Start: 725237
Stop: 724269
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG04049.1|AE004501_1 (AE004501) hypothetical protein [Pseudomonas aeruginosa PAO1]
Identities = 321/322 (99%), Positives = 321/322 (99%)
Sequence:
ATGTCCTTGCCCGCCCTGCTCGACCAACGCCTGCGACTGCCGATGGTGGCCGCGCCGATGTTCCTGGTATCC
AACCCGCAGTTGGTGCTGGCCTGCTGCCGCAGCGGCATCGTCGGCAGCTTCCCGGCGCTCAACCAGCGCGAG
AGCAGCGGCTTCGAGGGTTGGCTGGAGGAAATCGGGGCCGGCCTGCAAGCGCTGGACAATCCCGCGCCCTAT
GCGGTGAACCTGATCGTCCACAACACCAATCCGCGGTTGCAGGCCGACCTGCGGATCTGCGTCGAGCACCGC
GTACCGATCGTCATCACCAGCCTCGGCGCAGTGCGCGAAGTGGTAGACGCTGTGCATAGCTACGGCGGCCTG
GTATTCCACGACGTCACCACCCGCCGCCACGCCGAAAAGGCCGCCGAGGCCGGGGTCGACGGCCTGATCGCG
GTGGCGGCCGGCGCGGGCGGCCACGCCGGGACCTGGAGCCCTTTCGCGCTGGTCGCGGAAATCCGCCAGTTC
TTCGACAAGACTCTGCTGCTGGCCGGCTGCATCAACCACGGCCACGAGATTCTCGCCGCACAGATGCTCGGC
GCCGACCTGGCCTATCTCGGCACCCGCTTCATCGCCACCCGCGAGAGCAATGCCTCCGACGCCTACAAACAG
ATGATCCTGGAGGCACGCGCCGCCGACATCGTGCACACCCCGGCGGTGTCCGGCGTACCGGCCAGCTTCATG
CGGCAAAGCCTGGAGGCCGCCGGCTACGATCTGAAGCGCCTGACCGACAAGGCAGACATGAACTACGGCGAG
AAACTCAAGCCGGTGAACGACGAGGCCAAGGCCTGGAAGACCGTGTGGTCCGCCGGCCAGGGCGCGGGCAAC
ATAGACGACTTGCCCGGCGTCGACGAGTTGGTCGCCCGCCTCGACCGGGAATATCGAGACGCCCTGCGGAAC
ATGGATCGCCTGGCCAACCGCTGGCCGCGCTGA
Translation:
MSLPALLDQRLRLPMVAAPMFLVSNPQLVLACCRSGIVGSFPALNQRESSGFEGWLEEIGAGLQALDNPAPY
AVNLIVHNTNPRLQADLRICVEHRVPIVITSLGAVREVVDAVHSYGGLVFHDVTTRRHAEKAAEAGVDGLIA
VAAGAGGHAGTWSPFALVAEIRQFFDKTLLLAGCINHGHEILAAQMLGADLAYLGTRFIATRESNASDAYKQ
MILEARAADIVHTPAVSGVPASFMRQSLEAAGYDLKRLTDKADMNYGEKLKPVNDEAKAWKTVWSAGQGAGN
IDDLPGVDELVARLDREYRDALRNMDRLANRWPR*
AnnotationID:2211GeneID:2203
AnnotatorUID: diggins
Modification Date/Time: 2005-03-29 18:06:57
Gene Name:
Confidence Code:3
GeneProduct:putative 2-nitropropane dioxygenase
Cell Localization:()
Synonyms:PA0660
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(20) Putative enzymes
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG2070,pfam03060
Secondary Functional Category(ies):biosynthesis of cofactors, prosthetic groups and carriers
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAL53702.1| (AE009682) 2-NITROPROPANE DIOXYGENASE [Brucella melitensis 16M]
          Length = 339

 Score =  318 bits (814), Expect = 4e-86
 Identities = 167/315 (53%), Positives = 222/315 (70%), Gaps = 7/315 (2%)

>gb|AAN34006.1|AE014577_7 (AE014577) 2-nitropropane dioxygenase family protein [Brucella suis
           1330]
          Length = 322

 Score =  318 bits (814), Expect = 4e-86
 Identities = 167/315 (53%), Positives = 222/315 (70%), Gaps = 7/315 (2%)

>gb|AAP86144.1| (AY305378) putative 2-nitropropane dioxygenase [Ralstonia eutropha]
          Length = 325

 Score =  303 bits (776), Expect = 9e-82
 Identities = 170/324 (52%), Positives = 218/324 (67%), Gaps = 16/324 (4%)










 
Structural Features:
COG2070, Dioxygenases related to 2-nitropropane dioxygenase
CD-Length = 336 residues,  99.4% aligned
Score =  249 bits (638), Expect = 3e-67 
Identities = 114/336 (33%), Positives = 156/336 (46%), Gaps = 21/336 (6%)

pfam03060, NPD, 2-nitropropane dioxygenase
CD-Length = 325 residues,  95.4% aligned
Score =  123 bits (310), Expect = 3e-29
Identities = 87/317 (27%), Positives = 127/317 (40%), Gaps = 35/317 (11%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4409




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21555




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:34608




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2203_cdd.html


    		




 
URL:









           
Homologs By Global Alignment 

           

       



     
Gene ID:2203
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3750
                           PA0660_tr
                           translation of PA0660
                           322
                           99.68
                           99.68
                           0
                           1653.0
     

     
     
115714
                           gb|AAG04049.1|AE004501_1
                           (AE004501) hypothetical protein PA0660 [Pseudomonas aeruginosa PAO1]
                           322
                           99.68
                           99.68
                           0
                           1653.0
     

     
     
115715
                           gb|AAN66060.1|AE016234_13
                           (AE016775) conserved hypothetical protein [Pseudomonas putida KT2440]
                           322
                           78.26
                           84.78
                           4
                           1279.0
     

     
     
115716
                           emb|CAD13783.1|
                           (AL646058) CONSERVED HYPOTHETICAL PROTEIN [Ralstonia solanacearum]
                           338
                           52.66
                           67.75
                           27
                           837.0
     

     
     
115728
                           gb|AAP86144.1|
                           (AY305378) putative 2-nitropropane dioxygenase [Ralstonia eutropha]
                           338
                           51.77
                           65.97
                           29
                           796.0
     

     






      

          
GC ORFID: 45205How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA0660Glimmer Score: 

          
Start: 6526580Stop: 6525612

          
Length: 969


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA0660 translation of PA0660Homolog Bit Score 641.0

          
Other Homologs:  t_PA0660 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCTTGCCCGCCCTGCTCGACCAACGCCTGCGACTGCCGATGGTGGCCGCGCCGATGTTCCTGGTATCC 
AACCCGCAGTTGGTGCTGGCCTGCTGCCGCAGCGGCATCGTCGGCAGCTTCCCGGCGCTCAACCAGCGCGAG 
AGCAGCGGCTTCGAGGGTTGGCTGGAGGAAATCGGGGCCGGCCTGCAAGCGCTGGACAATCCCGCGCCCTAT 
GCGGTGAACCTGATCGTCCACAACACCAATCCGCGGTTGCAGGCCGACCTGCGGATCTGCGTCGAGCACCGC 
GTACCGATCGTCATCACCAGCCTCGGCGCAGTGCGCGAAGTGGTAGACGCTGTGCATAGCTACGGCGGCCTG 
GTATTCCACGACGTCACCACCCGCCGCCACGCCGAAAAGGCCGCCGAGGCCGGGGTCGACGGCCTGATCGCG 
GTGGCGGCCGGCGCGGGCGGCCACGCCGGGACCTGGAGCCCTTTCGCGCTGGTCGCGGAAATCCGCCAGTTC 
TTCGACAAGACTCTGCTGCTGGCCGGCTGCATCAACCACGGCCACGAGATTCTCGCCGCACAGATGCTCGGC 
GCCGACCTGGCCTATCTCGGCACCCGCTTCATCGCCACCCGCGAGAGCAATGCCTCCGACGCCTACAAACAG 
ATGATCCTGGAGGCACGCGCCGCCGACATCGTGCACACCCCGGCGGTGTCCGGCGTACCGGCCAGCTTCATG 
CGGCAAAGCCTGGAGGCCGCCGGCTACGATCTGAAGCGCCTGACCGACAAGGCAGACATGAACTACGGCGAG 
AAACTCAAGCCGGTGAACGACGAGGCCAAGGCCTGGAAGACCGTGTGGTCCGCCGGCCAGGGCGCGGGCAAC 
ATAGACGACTTGCCCGGCGTCGACGAGTTGGTCGCCCGCCTCGACCGGGAATATCGAGACGCCCTGCGGAAC 
ATGGATCGCCTGGCCAACCGCTGGCCGCGCTGA