ViewGene
Locus Name:
GeneID:
Location:

GeneID:220
TrivialName: PA14_29350
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_29350
PAO1 Ortholog Locus:PA2688
Sequence Length:2241
Protein Length:746
Start: 2541670
Stop: 2539430
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06076.1|AE004697_1 (AE004697) Ferric enterobactin receptor, outer membrane protein PfeA precursor [Pseudomonas aeruginosa PAO1]
Identities = 744/746 (99%), Positives = 744/746 (99%)
Sequence:
ATGTCCTCACGCGCCCTTCCCGCCGTTCCCTTCCTGCTGCTGTCCAGTTGCCTGCTCACCAACGCCGTACAC
GCCGCAGGCCAGGGCGACGGCTCCGTCATCGAGCTGGGCGAGCAGACCGTGGTCGCCACCGCCCAGGAGGAA
ACCAAGCAGGCACCGGGGGTTTCCATCATCACCGCCGAGGACATCGCCAAGCGACCGCCGAGCAACGACCTG
TCGCAGATCATCCGGACCATGCCGGGGGTCAACCTGACCGGCAACAGCTCCAGCGGCCAGCGTGGCAACAAC
CGGCAGATCGACATCCGCGGCATGGGCCCGGAGAACACCCTGATCCTGGTCGACGGCAAGCCGGTCAGCTCG
CGCAACTCGGTGCGCTACGGCTGGCGCGGCGAGCGCGACAGCCGCGGCGACACCAACTGGGTGCCGGCCGAC
CAGGTCGAGCGCATCGAAGTGATCCGCGGCCCGGCGGCGGCGCGCTACGGCAACGGCGCGGCGGGCGGCGTG
GTGAACATCATCACCAAGCAGGCCGGCGCGGAAACCCACGGTAATCTCAGCGTCTACAGCAATTTCCCGCAA
CACAAGGCCGAAGGCGCCAGCGAACGGATGAGCTTCGGTCTCAACGGGCCGCTCACGGAAAACCTCAGCTAC
CGCGTCTACGGCAACATCGCCAAGACCGACTCGGACGACTGGGACATCAACGCCGGCCACGAATCCAACCGC
ACCGGCAAGCAGGCCGGCACCCTCCCCGCCGGTCGCGAAGGCGTGCGCAACAAGGACATCGACGGGCTGCTC
AGCTGGCGCCTGACGCCCGAGCAGACCCTCGAGTTCGAGGCCGGCTTCAGCCGCCAGGGCAACATCTACACC
GGCGACACGCAGAACACCAACAGCAACAACTACGTGAAGCAGATGCTCGGCCACGAGACCAACCGCATGTAC
CGCGAGACCTACTCGGTCACCCATCGCGGCGAATGGGACTTCGGCAGCTCGCTGGCCTACCTGCAGTACGAG
AAGACCCGCAACAGCCGGATCAACGAAGGCCTGGCCGGCGGCACCGAAGGTATCTTCGACCCCAACAACGCC
GGCTTCTACACCGCCACCCTGCGCGACCTGACCGCCCACGGCGAGGTCAACCTGCCGCTGCACCTGGGCTAC
GAGCAGACCCTGACCCTCGGCAGCGAGTGGACCGAGCAGAAGCTCGACGACCCCAGCTCCAACACCCAGAAC
ACCGAGGAAGGCGGCTCGATCCCCGGTCTCGCCGGAAAGAACCGCAGCAGCAGTTCCTCGGCGCGGATCTTC
TCGCTGTTCGCCGAGGACAACATCGAGCTGATGCCCGGCACCATGCTCACCCCAGGCCTGCGCTGGGACCAC
CACGACATCGTCGGCGACAACTGGAGTCCATCGCTGAACCTGTCCCACGCGCTCACCGAGCGGGTCACCCTG
AAGGCCGGTATCGCCCGCGCCTACAAGGCCCCCAACCTGTACCAGTTGAACCCCGACTACCTGCTCTACAGC
CGTGGCCAGGGTTGCTACGGGCAAAGCACCAGTTGCTACCTGCGCGGCAACGACGGCCTCAAGGCCGAGACC
AGCGTGAACAAGGAACTGGGCATCGAGTACAGCCACGACGGCCTGGTAGCGGGGCTGACCTACTTCCGCAAC
GACTACAAGAACAAGATCGAATCCGGCCTGTCGCCGGTCGACCACGCCAGCGGCGGCAAGGGCGACTACGCC
AACGCGGCGATCTACCAGTGGGAGAACGTGCCCAAGGCGGTGGTCGAGGGCCTCGAAGGCACCCTGACCCTG
CCCCTGGCCGACGGCCTGAAGTGGAGCAACAACTTCACCTACATGCTGCAATCGAAGAACAAGGAAACCGGC
GACGTGCTCTCGGTGACGCCGCGCTACACCCTCAACTCGATGCTCGACTGGCAGGCCACCGACGACCTCTCA
CTGCAAGCCACGGTCACCTGGTACGGCAAGCAGAAGCCGAAGAAATACGACTATCACGGCGACCGTGTCACC
GGCAGCGCCAACGACCAGCTCTCGCCCTACGCCATCGCCGGCCTCGGCGGCACCTATCGGTTGAGCAAGAAC
CTGAGCCTCGGCGCCGGCGTCGACAACCTGTTCGACAAGCGCCTGTTCCGCGCCGGCAATGCCCAGGGCGTG
GTCGGCATCGACGGGGCCGGCGCGGCGACCTACAACGAGCCCGGACGGACCTTCTATACCAGCCTGACCGCG
TCGTTCTGA
Translation:
MSSRALPAVPFLLLSSCLLTNAVHAAGQGDGSVIELGEQTVVATAQEETKQAPGVSIITAEDIAKRPPSNDL
SQIIRTMPGVNLTGNSSSGQRGNNRQIDIRGMGPENTLILVDGKPVSSRNSVRYGWRGERDSRGDTNWVPAD
QVERIEVIRGPAAARYGNGAAGGVVNIITKQAGAETHGNLSVYSNFPQHKAEGASERMSFGLNGPLTENLSY
RVYGNIAKTDSDDWDINAGHESNRTGKQAGTLPAGREGVRNKDIDGLLSWRLTPEQTLEFEAGFSRQGNIYT
GDTQNTNSNNYVKQMLGHETNRMYRETYSVTHRGEWDFGSSLAYLQYEKTRNSRINEGLAGGTEGIFDPNNA
GFYTATLRDLTAHGEVNLPLHLGYEQTLTLGSEWTEQKLDDPSSNTQNTEEGGSIPGLAGKNRSSSSSARIF
SLFAEDNIELMPGTMLTPGLRWDHHDIVGDNWSPSLNLSHALTERVTLKAGIARAYKAPNLYQLNPDYLLYS
RGQGCYGQSTSCYLRGNDGLKAETSVNKELGIEYSHDGLVAGLTYFRNDYKNKIESGLSPVDHASGGKGDYA
NAAIYQWENVPKAVVEGLEGTLTLPLADGLKWSNNFTYMLQSKNKETGDVLSVTPRYTLNSMLDWQATDDLS
LQATVTWYGKQKPKKYDYHGDRVTGSANDQLSPYAIAGLGGTYRLSKNLSLGAGVDNLFDKRLFRAGNAQGV
VGIDGAGAATYNEPGRTFYTSLTASF*
AnnotationID:230GeneID:220
AnnotatorUID: diggins
Modification Date/Time: 2005-03-07 18:06:23
Gene Name:pfeA
Confidence Code:1
GeneProduct:ferric enterobactin receptor
Cell Localization:()
Synonyms:fepA
Cell Localization Confidence Code:5
MolecularFunction:ferrienterobactin receptor precursor
Functional Category:(15) Membrane proteins
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG4771,cd01347,pfam00593
Secondary Functional Category(ies):transport of small molecules
EC Number:
Status:ACTIVE
Pathway:






 
Homology:
>gb|AAO69884.1| (AE016841) ferrienterobactin receptor precursor [Salmonella
           enterica subsp. enterica serovar Typhi Ty2]
          Length = 751

 Score =  929 bits (2400), Expect = 0.0
 Identities = 457/721 (63%), Positives = 557/721 (77%), Gaps = 18/721 (2%)

>gb|AAL19536.1| (AE008723) outer membrane porin, receptor for ferric enterobactin
           (enterochelin) and colicins B and D [Salmonella
           typhimurium LT2]
          Length = 751

 Score =  927 bits (2397), Expect = 0.0
 Identities = 457/721 (63%), Positives = 557/721 (77%), Gaps = 18/721 (2%)

>gb|AAN79144.1|AE016757_48 (AE016757) Ferrienterobactin receptor precursor [Escherichia coli
           CFT073]
          Length = 746

 Score =  902 bits (2331), Expect = 0.0
 Identities = 442/720 (61%), Positives = 546/720 (75%), Gaps = 17/720 (2%)






 
Structural Features:
COG4771, FepA, Outer membrane receptor for ferrienterochelin and colicins 
[Inorganic ion transport and metabolism]
CD-Length = 699 residues,  99.9% aligned
Score =  700 bits (1806), Expect = 0.0
Identities = 395/749 (52%), Positives = 492/749 (65%), Gaps = 54/749 (7%)

cd01347, ligand_gated_channel, TonB dependent/Ligand-Gated channel
CD-Length = 672 residues,  99.9% aligned
Score =  254 bits (649), Expect = 3e-68

pfam00593, TonB_dep_Rec, TonB dependent receptor.
CD-Length = 712 residues,  99.9% aligned
Score =  225 bits (574), Expect = 1e-59
Identities = 206/763 (26%), Positives = 300/763 (39%), Gaps = 118/763 (15%)







   
Genomic Context:






   
Comment:


















    ReferenceID:443




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:17585




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:36331




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:220_cdd.html


    		




 
URL:

















    ReferenceID:36363




  
Author/Investigator(s):
        Dean CR, Poole K.





  
Title:
        Cloning and characterization of the ferric enterobactin receptor gene (pfeA) of Pseudomonas aeruginosa.




  
PubMed:
        8419284


MedLine:
        




    
Source:
J Bacteriol. 1993 Jan;175(2):317-24.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=8419284









           
Homologs By Global Alignment 

           

       



     
Gene ID:220
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
385
                           PA2688_tr
                           translation of PA2688
                           746
                           99.73
                           99.73
                           0
                           3911.0
     

     
     
22360
                           gb|AAG06076.1|AE004697_1
                           (AE004697) Ferric enterobactin receptor, outer membrane protein PfeA precursor [Pseudomonas aeruginosa PAO1]
                           746
                           99.73
                           99.73
                           0
                           3911.0
     

     
     
22361
                           gb|AAA25928.1|
                           (M98033) ferric enterobactin receptor [Pseudomonas aeruginosa]
                           746
                           99.73
                           99.73
                           0
                           3911.0
     

     
     
22362
                           gb|AAO69884.1|
                           (AE016841) ferrienterobactin receptor precursor [Salmonella enterica subsp. enterica serovar Typhi Ty2]
                           773
                           60.02
                           73.22
                           49
                           2422.0
     

     
     
22363
                           emb|CAD05061.1|
                           (AL627267) ferrienterobactin receptor precursor [Salmonella enterica subsp. enterica serovar Typhi]
                           773
                           60.02
                           73.22
                           49
                           2422.0
     

     






      

          
GC ORFID: 43222How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA2688Glimmer Score: 

          
Start: 2412256Stop: 2414496

          
Length: 2241


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA2688 translation of PA2688Homolog Bit Score 1511.0

          
Other Homologs:  t_PA0931 (ORF: BLASTX 837.0), t_PA2688 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGTCCTCACGCGCCCTTCCCGCCGTTCCCTTCCTGCTGCTGTCCAGTTGCCTGCTCACCAACGCCGTACAC 
GCCGCAGGCCAGGGCGACGGCTCCGTCATCGAGCTGGGCGAGCAGACCGTGGTCGCCACCGCCCAGGAGGAA 
ACCAAGCAGGCACCGGGGGTTTCCATCATCACCGCCGAGGACATCGCCAAGCGACCGCCGAGCAACGACCTG 
TCGCAGATCATCCGGACCATGCCGGGGGTCAACCTGACCGGCAACAGCTCCAGCGGCCAGCGTGGCAACAAC 
CGGCAGATCGACATCCGCGGCATGGGCCCGGAGAACACCCTGATCCTGGTCGACGGCAAGCCGGTCAGCTCG 
CGCAACTCGGTGCGCTACGGCTGGCGCGGCGAGCGCGACAGCCGCGGCGACACCAACTGGGTGCCGGCCGAC 
CAGGTCGAGCGCATCGAAGTGATCCGCGGCCCGGCGGCGGCGCGCTACGGCAACGGCGCGGCGGGCGGCGTG 
GTGAACATCATCACCAAGCAGGCCGGCGCGGAAACCCACGGTAATCTCAGCGTCTACAGCAATTTCCCGCAA 
CACAAGGCCGAAGGCGCCAGCGAACGGATGAGCTTCGGTCTCAACGGGCCGCTCACGGAAAACCTCAGCTAC 
CGCGTCTACGGCAACATCGCCAAGACCGACTCGGACGACTGGGACATCAACGCCGGCCACGAATCCAACCGC 
ACCGGCAAGCAGGCCGGCACCCTCCCCGCCGGTCGCGAAGGCGTGCGCAACAAGGACATCGACGGGCTGCTC 
AGCTGGCGCCTGACGCCCGAGCAGACCCTCGAGTTCGAGGCCGGCTTCAGCCGCCAGGGCAACATCTACACC 
GGCGACACGCAGAACACCAACAGCAACAACTACGTGAAGCAGATGCTCGGCCACGAGACCAACCGCATGTAC 
CGCGAGACCTACTCGGTCACCCATCGCGGCGAATGGGACTTCGGCAGCTCGCTGGCCTACCTGCAGTACGAG 
AAGACCCGCAACAGCCGGATCAACGAAGGCCTGGCCGGCGGCACCGAAGGTATCTTCGACCCCAACAACGCC 
GGCTTCTACACCGCCACCCTGCGCGACCTGACCGCCCACGGCGAGGTCAACCTGCCGCTGCACCTGGGCTAC 
GAGCAGACCCTGACCCTCGGCAGCGAGTGGACCGAGCAGAAGCTCGACGACCCCAGCTCCAACACCCAGAAC 
ACCGAGGAAGGCGGCTCGATCCCCGGTCTCGCCGGAAAGAACCGCAGCAGCAGTTCCTCGGCGCGGATCTTC 
TCGCTGTTCGCCGAGGACAACATCGAGCTGATGCCCGGCACCATGCTCACCCCAGGCCTGCGCTGGGACCAC 
CACGACATCGTCGGCGACAACTGGAGTCCATCGCTGAACCTGTCCCACGCGCTCACCGAGCGGGTCACCCTG 
AAGGCCGGTATCGCCCGCGCCTACAAGGCCCCCAACCTGTACCAGTTGAACCCCGACTACCTGCTCTACAGC 
CGTGGCCAGGGTTGCTACGGGCAAAGCACCAGTTGCTACCTGCGCGGCAACGACGGCCTCAAGGCCGAGACC 
AGCGTGAACAAGGAACTGGGCATCGAGTACAGCCACGACGGCCTGGTAGCGGGGCTGACCTACTTCCGCAAC 
GACTACAAGAACAAGATCGAATCCGGCCTGTCGCCGGTCGACCACGCCAGCGGCGGCAAGGGCGACTACGCC 
AACGCGGCGATCTACCAGTGGGAGAACGTGCCCAAGGCGGTGGTCGAGGGCCTCGAAGGCACCCTGACCCTG 
CCCCTGGCCGACGGCCTGAAGTGGAGCAACAACTTCACCTACATGCTGCAATCGAAGAACAAGGAAACCGGC 
GACGTGCTCTCGGTGACGCCGCGCTACACCCTCAACTCGATGCTCGACTGGCAGGCCACCGACGACCTCTCA 
CTGCAAGCCACGGTCACCTGGTACGGCAAGCAGAAGCCGAAGAAATACGACTATCACGGCGACCGTGTCACC 
GGCAGCGCCAACGACCAGCTCTCGCCCTACGCCATCGCCGGCCTCGGCGGCACCTATCGGTTGAGCAAGAAC 
CTGAGCCTCGGCGCCGGCGTCGACAACCTGTTCGACAAGCGCCTGTTCCGCGCCGGCAATGCCCAGGGCGTG 
GTCGGCATCGACGGGGCCGGCGCGGCGACCTACAACGAGCCCGGACGGACCTTCTATACCAGCCTGACCGCG 
TCGTTCTGA