ViewGene
Locus Name:
GeneID:
Location:

GeneID:2197
TrivialName: PA14_20950
AnnotatorUID:
Modification Date/Time:2006-04-06 13:01:23
LocusName:PA14_20950
PAO1 Ortholog Locus:PA3333
Sequence Length:993
Protein Length:330
Start: 1804538
Stop: 1803546
Strand: -
Type:
ChromosomeID:2
Status: ACTIVE
Frame Discrepancy: FALSE
Missense Discrepancy:FALSE
Comments:
Homology: gb|AAG06721.1|AE004755_8 (AE004755) 3-oxoacyl-[acyl-carrier-protein] synthase III [Pseudomonas aeruginosa PAO1]
Identities = 330/330 (100%), Positives = 330/330 (100%)
Sequence:
ATGCCGCGCGCCGCCGTGGTCTGCGGCCTGGGCAGCTACCTGCCCGAGGCCGTGCTCAGCAACGACATGCTC
GCCGCCGAGCTGGACACTTCCGACGCCTGGATCAGCAGCCGCACCGGCGTGCGCCAGCGGCATATCGCCGGC
GACCTCGGCAGCGGCGACCTGGCCCTGCGGGCGGCCTCCGCCGCGCTCGCCTCGGCGGGGCTGGAGCGAGTC
GACGCGGTGGTGCTGGCAACCAGCACCGGCGACTTCTGCTGCCCGGCCACCGCGCCCAGGGTCGCGGCGCGC
CTGGGGTTGGTCGGCGCGCTCGCGTTCGACCTGTCCGCCGCCTGCACCGGCTTCGTCTACGGCCTGGCCAGC
GTCGGCTCGCTGATCAGCGCCGGGCTGGCGGACAGCGCGCTGCTGGTCGGGGTGGACACTTTCAGCCATACC
CTCGACCCCGCCGATCGCTCGACCCGCGCACTGTTCGGCGACGGCGCCGGAGCGGTGGTGCTGCGTGCCGGC
GATGCCGAGGAAGAAGGCGCGCTGCTGGCCTTCGACCTCGGCAGCGACGGCCACCAGTTCGACCTGCTGATG
ACCCCCGCCGTGAGTCGCGCCGAACGCAGTTCCGGACAGGCCTCCAACTACTTCCGGATGGACGGCAAGGCA
GTGTTCGGCCAGGCGGTGACGCAGATGAGCGACTCGGTGCGGCGGGTGCTCGACCGGGTCGGCTGGCAAGCT
TCGGACCTCCATCACCTGGTCCCGCACCAGGCCAACACACGCATTCTCGCGGCGGTCGCCGACCAGCTCGAC
CTTCCCGTCGAGCGAGTGGTGAGCAACATCGCCGAGGTGGGCAATACCGTCGCCGCCTCGATTCCCCTGGCC
CTGGCCCACGGCCTGCGCCAAGGCATCCTGCGCGACGGCGGCAACATGGTCCTCACCGGTTTCGGTGCCGGA
CTGACCTGGGGTTCGGTCGCCCTGCGCTGGCCGAAGATCGTTCCGACAATGGACTGA
Translation:
MPRAAVVCGLGSYLPEAVLSNDMLAAELDTSDAWISSRTGVRQRHIAGDLGSGDLALRAASAALASAGLERV
DAVVLATSTGDFCCPATAPRVAARLGLVGALAFDLSAACTGFVYGLASVGSLISAGLADSALLVGVDTFSHT
LDPADRSTRALFGDGAGAVVLRAGDAEEEGALLAFDLGSDGHQFDLLMTPAVSRAERSSGQASNYFRMDGKA
VFGQAVTQMSDSVRRVLDRVGWQASDLHHLVPHQANTRILAAVADQLDLPVERVVSNIAEVGNTVAASIPLA
LAHGLRQGILRDGGNMVLTGFGAGLTWGSVALRWPKIVPTMD*
AnnotationID:2205GeneID:2197
AnnotatorUID: diggins
Modification Date/Time: 2005-04-15 09:09:01
Gene Name:fabH2
Confidence Code:2
GeneProduct:3-oxoacyl-[acyl-carrier-protein] synthase III
Cell Localization:()
Synonyms:
Cell Localization Confidence Code:5
MolecularFunction:
Functional Category:(13) Fatty acid and phospholipid metabolism
Alternate Gene Product Name:
Functional Category Confidence Code:5
COGs:COG0332
Secondary Functional Category(ies):
EC Number:2.3.1.41
Status:ACTIVE
Pathway:






 
Homology:
>emb|CAB82875.1| (AJ276673) putative condensing enzyme [Streptomyces coelicolor
           A3(2)]
          Length = 332

 Score =  326 bits (836), Expect = 1e-88
 Identities = 171/329 (51%), Positives = 220/329 (66%), Gaps = 4/329 (1%)

emb|CAC36759.1| (AL590464) putative acyl carrier protein synthase, MmyC
           [Streptomyces coelicolor A3(2)]
          Length = 332

 Score =  326 bits (836), Expect = 1e-88
 Identities = 171/329 (51%), Positives = 220/329 (66%), Gaps = 4/329 (1%)

>dbj|BAC70001.1| (AP005030) putative 3-oxoacyl-ACP synthase III [Streptomyces
           avermitilis MA-4680]
          Length = 335

 Score =  312 bits (800), Expect = 2e-84
 Identities = 168/330 (50%), Positives = 221/330 (66%), Gaps = 5/330 (1%)






 
Structural Features:
COG0332, FabH, 3-oxoacyl-[acyl-carrier-protein]
CD-Length = 323 residues,  99.1% aligned
Score =  342 bits (880), Expect = 2e-95
Identities = 152/322 (47%), Positives = 200/322 (62%), Gaps = 5/322 (1%)






   
Genomic Context:






   
Comment:


















    ReferenceID:4397




  
Author/Investigator(s):
        GenNotator





  
Title:
        




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:BLASTP 







    
Data:


    		




 
URL:

















    ReferenceID:21543




  
Author/Investigator(s):
        GenNotator





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:RPSBLAST 







    
Data:


    		




 
URL:

















    ReferenceID:32231




  
Author/Investigator(s):
        





  
Title:
        CDD Search




  
PubMed:
        


MedLine:
        




    
Source:






    
Reference Type:Misc (text/plain)







    
Data:2197_cdd.html


    		




 
URL:

















    ReferenceID:34002




  
Author/Investigator(s):
        Kiatpapan P, Kobayashi H, Sakaguchi M, Ono H, Yamashita M, Kaneko Y, Murooka Y.





  
Title:
        Molecular characterization of Lactobacillus plantarum genes for beta-ketoacyl-acyl carrier protein synthase III (fabH) and acetyl coenzyme A carboxylase (accBCDA), which are essential for fatty acid biosynthesis.




  
PubMed:
        11133475


MedLine:
        




    
Source:
Appl Environ Microbiol. 2001 Jan;67(1):426-33.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=11133475

















    ReferenceID:34003




  
Author/Investigator(s):
        Zhang Y, Cronan JE Jr.





  
Title:
        Transcriptional analysis of essential genes of the Escherichia coli fatty acid biosynthesis gene cluster by functional replacement with the analogous Salmonella typhimurium gene cluster.




  
PubMed:
        9642179


MedLine:
        




    
Source:
J Bacteriol. 1998 Jul;180(13):3295-303.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=9642179

















    ReferenceID:34004




  
Author/Investigator(s):
        Tsay JT, Oh W, Larson TJ, Jackowski S, Rock CO.





  
Title:
        Isolation and characterization of the beta-ketoacyl-acyl carrier protein synthase III gene (fabH) from Escherichia coli K-12.




  
PubMed:
        1551888


MedLine:
        




    
Source:
J Biol Chem. 1992 Apr 5;267(10):6807-14.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1551888

















    ReferenceID:34005




  
Author/Investigator(s):
        Carty SM, Colbeau A, Vignais PM, Larson TJ.





  
Title:
        Identification of the rpmF-plsX-fabH genes of Rhodobacter capsulatus.




  
PubMed:
        8020746


MedLine:
        




    
Source:
FEMS Microbiol Lett. 1994 May 15;118(3):227-31.






    
Reference Type:Journal 







    
Data:


    		




 
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=8020746









           
Homologs By Global Alignment 

           

       



     
Gene ID:2197
           
Identity:

                               
                                   0%
                               
                                   10%
                               
                                   20%
                               
                                   30%
                               
                                   40%
                               
                                   50%
                               
                                   60%
                               
                                   70%
                               
                                   80%
                               
                                   90%
                               
                                   100%
                               
                             

                           

     
HomologID
                           Accession
                           Description
                           Length
                           PctIdentity
                           PctSimilarity
                           Gaps
                           Score
     

     
     
3743
                           PA3333_tr
                           translation of PA3333
                           330
                           99.99
                           99.99
                           0
                           1655.0
     

     
     
115517
                           gb|AAG06721.1|AE004755_8
                           (AE004755) 3-oxoacyl-[acyl-carrier-protein] synthase III [Pseudomonas aeruginosa PAO1]
                           330
                           99.99
                           99.99
                           0
                           1655.0
     

     
     
115522
                           emb|CAA16179.1|
                           (AL939125) 3-oxoacyl-[acyl-carrier-protein] synthase [Streptomyces coelicolor A3(2)]
                           338
                           52.95
                           65.97
                           11
                           830.0
     

     
     
115519
                           emb|CAC36759.1|
                           (AL590464) putative acyl carrier protein synthase, MmyC [Streptomyces coelicolor A3(2)]
                           334
                           51.19
                           65.86
                           6
                           837.0
     

     
     
115518
                           emb|CAB82875.1|
                           (AJ276673) putative condensing enzyme [Streptomyces coelicolor A3(2)]
                           334
                           51.19
                           65.86
                           6
                           837.0
     

     






      

          
GC ORFID: 45199How Found: BLASTX

          
GC_TrimmedSeqID: 69Blast Result ID

          
Subject Sequence Name: t_PA3333Glimmer Score: 

          
Start: 3149394Stop: 3150386

          
Length: 993


          
Start Codon: ATGTruncated Start:

          
Stop Codon: TGATruncated Stop: 

          
Homolog: t_PA3333 translation of PA3333Homolog Bit Score 642.0

          
Other Homologs:  t_PA3333 (ORF: GLIMMER)

          
GC ORF Sequence

           
ATGCCGCGCGCCGCCGTGGTCTGCGGCCTGGGCAGCTACCTGCCCGAGGCCGTGCTCAGCAACGACATGCTC 
GCCGCCGAGCTGGACACTTCCGACGCCTGGATCAGCAGCCGCACCGGCGTGCGCCAGCGGCATATCGCCGGC 
GACCTCGGCAGCGGCGACCTGGCCCTGCGGGCGGCCTCCGCCGCGCTCGCCTCGGCGGGGCTGGAGCGAGTC 
GACGCGGTGGTGCTGGCAACCAGCACCGGCGACTTCTGCTGCCCGGCCACCGCGCCCAGGGTCGCGGCGCGC 
CTGGGGTTGGTCGGCGCGCTCGCGTTCGACCTGTCCGCCGCCTGCACCGGCTTCGTCTACGGCCTGGCCAGC 
GTCGGCTCGCTGATCAGCGCCGGGCTGGCGGACAGCGCGCTGCTGGTCGGGGTGGACACTTTCAGCCATACC 
CTCGACCCCGCCGATCGCTCGACCCGCGCACTGTTCGGCGACGGCGCCGGAGCGGTGGTGCTGCGTGCCGGC 
GATGCCGAGGAAGAAGGCGCGCTGCTGGCCTTCGACCTCGGCAGCGACGGCCACCAGTTCGACCTGCTGATG 
ACCCCCGCCGTGAGTCGCGCCGAACGCAGTTCCGGACAGGCCTCCAACTACTTCCGGATGGACGGCAAGGCA 
GTGTTCGGCCAGGCGGTGACGCAGATGAGCGACTCGGTGCGGCGGGTGCTCGACCGGGTCGGCTGGCAAGCT 
TCGGACCTCCATCACCTGGTCCCGCACCAGGCCAACACACGCATTCTCGCGGCGGTCGCCGACCAGCTCGAC 
CTTCCCGTCGAGCGAGTGGTGAGCAACATCGCCGAGGTGGGCAATACCGTCGCCGCCTCGATTCCCCTGGCC 
CTGGCCCACGGCCTGCGCCAAGGCATCCTGCGCGACGGCGGCAACATGGTCCTCACCGGTTTCGGTGCCGGA 
CTGACCTGGGGTTCGGTCGCCCTGCGCTGGCCGAAGATCGTTCCGACAATGGACTGA